• Applied Chemistry for Engineering
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• v.31 no.6
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• pp.612-623
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• 2020

This study was initiated to quantitatively evaluate the inhibitory effects of five heavy metals (Cd, Cu, Zn, Pb, Ni) on bacterial growth and phosphorus removal in the binary culture of Alcaligenes sp. plus Pseudomonas sp. IC50 values of Alcaligenes sp. plus Pseudomonas sp. for Cd, Cu, Zn, Pb, and Ni were 0.75, 10.93, 7.08, 13.30, and 15.78 mg/L, respectively. For the binary treatments of heavy metals, IC50 was the lowest in the treatment of Cd + Cu, whereas, it was the highest in the Ni + Pb treatment. The EC50 values for Cd, Cu, Zn, Pb, and Ni were 0.54, 11.08, 6.14, 9.33, and 13.81 mg/L, respectively. For the binary treatments of heavy metals, EC50 was the lowest in the Cd + Zn, whereas, the highest in the Zn + Ni. Based on both IC50 and EC50 values for the binary culture of bacteria with the binary mixtures of heavy metals, the most interactive effect was found to be antagonistic, though the only synergistic effect was found in Cu + Ni treatment. Therefore, our results can provide basic data on the toxic effects of heavy metals on the bacterial growth and phosphorus removal in the wastewater treatment process.

• 한국생태학회:학술대회논문집
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• 1998.05a
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• pp.122.1-122.1
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• 1998

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.6
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• pp.540-545
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• 2005

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3HB-co-3HV), copolyesters, with 3-hydroxyvalerate (3HV) contents ranging from 17 to 60 mol%, were produced by Alcaligenes sp. MT-16, and their biocompatibility evaluated by the growth of Chinese hamster ovary (CHO) cells and the adsorption of blood proteins and platelets onto their film surfaces. The number of CHO cells that adhered to and grew on these films was higher with increasing 3HV content. In contrast, the tendency for blood proteins and platelets to adhere to the copolyester surfaces significantly decreased with increasing 3HV content. Examination of the surface morphology using atomic force microscopy revealed that the surface roughness was an important factor in determining the biocompatibility of theses copolyesters. The results obtained in this study suggest that poly(3HB-co-3HV) copolyesters, with >30 mol% 3HV, may be useful in biocompatible biomedical applications.

• Textile Coloration and Finishing
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• v.7 no.1
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• pp.51-57
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• 1995

Poly-$\beta$-hydroxybutyrate(PHB) was biosynthesized using Alcaligenes sp. FL-027. Alcaligenes sp. FL-027 was cultivated by fed-batch methods, in order to promote cell growth and PHB accumulation with carbon source. The cells were first grown at 3$0^{\circ}C$ on the fermentor. The structure of biosynthesized PHB is investigated by the NMR, IR. The crystalline portions were identified through the use of DSC and X-ray diffractometer. The melting point was about 16$0^{\circ}C$ and the diffraction peaks of (020) and (110) were shown at 13$^{\circ}$ and 17$^{\circ}$, respectively.

• Journal of Microbiology and Biotechnology
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• v.9 no.6
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• pp.722-725
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• 1999

Filamentation-suppressed recombinant Escherichia coli strain harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes and the E. coli ftsZ gene was constructed and cultivated for the production of poly(3-hydroxybutyrate) [P(3HB)] with high concentration and high content. By the pH-stat fed-batch culture of this recombinant E. coli strain XL1-Blue(pJC5), the final cell concentration and P(3HB) concentration obtained in 44.25h were 172.2g cell dry weight/l and 141.9g P(3HB)/l, respectively, resulting in productivity of 3.21g P(3HB)/l-h. More importantly, the P(3HB) content obtained was 82.4 wt %, which was significantly higher than that obtained with the recombinant E. coli harboring only the PHA biosynthesis genes.

• Journal of Microbiology
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• v.34 no.3
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• pp.279-283
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• 1996

The weight average molecular weight of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesized by Alcaligenes sp. SH-69 was altered between $3.2$\times$10^5$ and $1.1$\times$10^6$ depending upon various culture conditions. It appeared that culture conditions favorable for the efficient production of copolyesters promoted the formation of higher molecular weight copolyesters. Polydispersity indices of isolated copolyesters were in the range of 1.5 to 2.5.

• Korean Journal of Microbiology
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• v.26 no.3
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• pp.247-255
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• 1988

Using the numerical clustering method, 14 clusters (containing three or more strains) among 231 bacterial isolates from St. 1,17 clusters among 252 isolates from St.2,17 clusters among 301 isolates from St.3, and 15 clusters among 260isolates from St.4 were found at the 70% similarity value. The predominant organisms were identified as genera Aeromonas, Vibrio, and Alcaligenes at St.1, Alcaligenes at St.2, Aeromonas, Vibrio, and Moraxella-Acinetobacter group at St.3, and Pseudomonas at St.4.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.716-716
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• 2003

• Proceedings of the Microbiological Society of Korea Conference
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• 2002.10a
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• pp.222.2-222.2
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• 2002

• Proceedings of the Zoological Society Korea Conference
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• 1996.10a
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• pp.196.2-196
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• 1996

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