• Korean Journal of Plant Taxonomy
• /
• v.43 no.3
• /
• pp.165-167
• /
• 2013

Five taxa of the genus Ajuga L. (Lamiaceae) were known previously in Korea which were mainly distributed in lowland and lower montane areas at 50-1000 m elevation. We report an unrecorded taxon of the genus Ajuga in Korea, namely Ajuga nipponensis Makino. This taxon was discovered at Is. Nok, Boryeong-si, in Chungcheongnam Province. A. nipponensis is found to be distributed along roadsides near villages of the island. A. nipponensis was distinguishable from other Ajuga in Korea by following combination of characters: Stems erect or procumbent, arranged in a dense cluster, corolla whitish-pale pink, 10-12 mm long. This taxon was named 'Bun-hong-kkot-jo-gae-na-mul' in Korean based on color of the corolla. The key to the genus Ajuga in Korea is also provided.

• Korean Journal of Pharmacognosy
• /
• v.29 no.4
• /
• pp.318-322
• /
• 1998

Continuing to previous report, seven compounds were isolated from the aerial parts of Ajuga multiflora. The structures of them were established as Di-2-ethylhexyl phthalate (1), ursolic acid (2), sterol glucoside (3), 20-hydroxyecdysone (4), makisterone A (5). cyasterone (6) and apigenin 7-glucuronide (7), respectively.

• Korean Journal of Pharmacognosy
• /
• v.16 no.3
• /
• pp.160-164
• /
• 1985

Ajuga multiflora Bunge is a perennial herb in the family of Labiatae. The iridoid glycoside was isolated from the ethylacetate fraction and the n-butanol fraction of the plant. The structure of the substance was identified as 8-O-acetylharpagide by UV, IR, NMR spectra and several chemical reactions.

• Korean Journal of Pharmacognosy
• /
• v.29 no.2
• /
• pp.75-78
• /
• 1998

In the course of phytochemical studies for the aerial parts of Ajuga multiflora, one flavonoid and two iridoid glycosides were isolated and identified as apigenin (1), 8-O-acetylharpagide (2) and harpagide (3) on the basis of spectroscopic evidence.

• Korean Journal of Pharmacognosy
• /
• v.31 no.1
• /
• pp.72-76
• /
• 2000

This study was carried out to evaluate cytotoxic effects of Ajuga multiflora Bunge extracts on murine leukemia tumor $(P388D_1)$ cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. The comparison of $IC_{50}$ values of Ajuga multiflora Bunge extracts in L1210 and $P388D_1$ cell lines showed that their susceptibility to these extracts decreased in the following order: Adriamycin>methanol extract>chloroform extract>ethyl acetate extract>hexane extract>water extract by the MTT assay. In order to develop an antimicrobial agent, dried Ajuga multiflora Bunge was extracted with several solvents, and then antimicrobial activity was investigated. The minimal inhibitory concentration (MIC) of the extract against microorganisms were also examined. Antimicrobial activities of amocla and ketoconazole as references were compared to those of extracts of $H_2O$, n-hexane, chloroform, ethyl acetate and methanol. The antimicrobial activity of all extracts from the sample had growth inhibition activity against gram-negative bacteria, gram-positive bacteria and fungi $(MIC>200\;{\mu}g/ml)$. These results suggest that the methanol soluble extract of Ajuga multiflora Bunge may be a valuable choice for the studies on the treatment of murine leukemia tumor cell lines.

• 한국약용작물학회:학술대회논문집
• /
• 2007.05a
• /
• pp.263-264
• /
• 2007

• Journal of agriculture & life science
• /
• v.44 no.6
• /
• pp.61-67
• /
• 2010

Six evergreen ground-cover species including Ajuga reptans, Carex morrowii 'Variegata', Dianthus 'Night Star', Hedera helix 'Ivalace', Pachysandra terminalis, and Sedum rupestre, were tested for cold acclimation with three different light treatments (0, 30, and 70% of shading, respectively) to select potential candidates for over-wintering in central Korea. Among those species, Ajuga reptans, Carex morrowii 'Variegata', and Sedum rupestre performed well, which had more than 50% of survival rate in all light conditions. However, Dianthus 'Night Star' had less than 40% under any light treatment and the other two species, Hedera helix 'Ivalace' and Pachysandra terminalis, survived more than 40% only with 70% of shading condition. These results imply that those candidate species having less cold hardness in central Korea could have the potential to could over-winter as long as a proper light condition is provided.

• Asian Journal of Turfgrass Science
• /
• v.7 no.1
• /
• pp.19-30
• /
• 1993

Experiments were conducted to investigate propagation, shade tolerance, cold resistance and growth rate of evergreen ground cover plants ; Vinca minor K , Ajuga reptans L., Ophiopogon japonicus ker. f nanus hort , and Hedera helix.. The results were as follows : 1.It is convenient to use the growing case for rice seed to grow and transplant Vinca minor seed-ling. The most proper density of transplanting Vinea was 180 plants per 1m$^2$. At the end of growing season, the coverage came up to 90% The rooting ability of Hedera helix was best to cut from April to May (temperature 15~23˚C). The seedling length of Parthenocissus quinguefohlia were irregular and ranged from 5 cm of 200cm. 2. Ajuga reptans L., Ophiopogon j. and Vinca minor L. grew better under 50% light intensity than full sunlight. Particularly, under full sunlight and aestival high temperature, there were a few withering plants in Ajuga reptans L. , and V Vilica minor L. 3. Of Vinca minor K., Ajuga reptans L. , Ophiopogon japonicus ker. f. nanus hort. and Hedera helix. acclimatized in open field, where temperature was from -7~8˚C. There were few plants had damage in low temperature incubator to -16˚C after field acclimatization. 4.Tendrils of Parthenocissus quinguefolia strongly clung to the wire netting stone fence but intruded into the gap and cranny of the precast concrete fence and so clung to, so that it needed to install the bending net on the precast concrete fence.

• YAKHAK HOEJI
• /
• v.14 no.3_4
• /
• pp.51-53
• /
• 1970

Alcohol extracts of six plant namely Coix agrestis L$_{OUREIRO}$, Chloranthus Japonicus (Siebi) N$_{AKAI}$, Ajuga Spectabilis N$_{AKAI}$, Juglans mandsuria Max., Cirsium Macki var Ussuriense $K_{ITA}$ and Sasamorpha Purpurascense (Hack) N$_{AKAI}$ were subjected to the screening tests for anti-tumor activities against SN-36 Leukemia, Sarcoma 180 and Ehrlich ascites carcinoma. Of the six extracts, Ajuga Spectabilis N$_{AKAI}$ and Coix agrestis L$_{OUREIRO}$ were found to be potential in survival effect of tumor transplanted mice.

• Applied Biological Chemistry
• /
• v.41 no.4
• /
• pp.251-256
• /
• 1998

The analytical method of ${\beta}-ecdysone$, the insect moulting hormone, by high performance liquid chromatograph (HPLC) with UV detector was developed using boronic ester derivatization and applied to the extracts of Ajuga iva, Silene otites and Schistocerca egg. Derivatization of yield with methyl-, butyl-, and phenyl-boronate was completed under mild conditions with 20-hydroxyecdysone. The conversion ratios of boronate were estimated to be 70% in methylboronic acid, 89% in butylboronic acid and 93% in phenylboronic acid. Phenylboronate showed a high sensitivity and demonstrated an effective separation on HPLC. The optimum temperature and reaction time for derivative formation were $25^{\circ}C$ and 20 min. respectively. ${\beta}-Ecdysone$ was effectively identified in extracts of Ajuga iva, Silene otites and Schistocerca egg by the HPLC method.