• Korean Journal of Pharmacognosy
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• v.29 no.1
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• pp.13-17
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• 1998

The root of Aralia continentalis Kitagawa (Araliaceae) have been used as an analgesic and fever remedy, and for treatment of rheumatism in Chinese medicine, whereas the young leaves are used for ingredient of salad. Antioxidant activity of the young leaves of A. continentails was determined by measuring lipid peroxide produced when a mouse liver homogenate was exposed to the air at $37^{\circ}C$, using 2-thiobarbituric acid (TBA) and by evaluation the radical scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. Chromatographic separation of active fraction led to the isolation of six flavonoids, among which quercetin, hyperoside and kaempferol showed strong antioxidant activities, while 6"-O-acetyl astragalin, astragalin and trifolin were inactive. Adenosine, oleanolic acid 28-O-glucosyl ester and salsoloside C methyl ester isolated from the somewhat active BuOH fraction exhibited no antioxidant activities.

• Journal of Radiation Protection and Research
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• v.2 no.1
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• pp.31-37
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• 1977

Inactivation of the glutamic acid dehydrogenase and glucose-6-phosphate dehydrogenase enzyme-molecules in the Ehrlich ascites tumor cells of the mouse were studied. The above mentioned intracellular enzymemolecules were irradiated by the X-ray radiation under the condition of 65 kV, I Amp. under the atmosphere of nitrogen gases and by $4^{\circ}C$. Thereby, irradiation doses were 580 KR/min($error:{\pm}3%$). After irradiation, the cell homogentes were prepared through liquid air techniquese. There after, the activities of the enzymes were measured with photometric method given by O. Warburg and W. Christian. The dose effect curves of the activities of the two enzymes by the X-ray irradiation showed both exponential and the inactivation doses were $6,5.10^{0}\;and\;5,0.10^{6}$ R respectively. These results showed one side that the inactivation process of the intracelluar enzymemolecules was one hit reaction after target theory, and the other side that this inactivation process could not be the primary causes of the death through X-ray irradiation of the vertebrate animals, because of the high resistance of the intracellular protein molecules against X-ray irradiation. The one hit reaction by the inactivation process of the irradiated intracellular enzymemolecules was discussed.

• Archives of Pharmacal Research
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• v.22 no.2
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• pp.213-218
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• 1999

The antioxidant activity of Eriobotrya japonica was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and lipid peroxidation produced when mouse liver homogenate was exposed to the air at $37^{\circ}C$, using 2-thiobarbituric acid (TBA). The methanol extract and its factions of Eriobotrya japonica leaves showed strong antioxidant activity. The antioxidant activity of EtOAc and n-BuOH soluble fractions were stronger than the others, and were further purified by repeated silica gel, MCl gel CHP-20P, and Sephadex LH-20 column chromatography. Antioxidant chlorogenic acid, quercetin-3-sambubioside from n-BuOH fraction and methyl chlorogenate, kaempferol- and quercetin-3-rhamnosides, together with the inactive ursolic acid and$ 2{\alpha}$-hydroxyursolic acid from EtOAc fraction were isolated. Antioxidant flavonoids and chlorogenic acid also showed prominent inhibitory activity against free radical generation in dichlorofluorescein (DCF) method.

• Clinical and Experimental Reproductive Medicine
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• v.28 no.3
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• pp.183-190
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• 2001

Objective: Recently, microdissection of tissue sections has been used increasingly for the isolation of morphologically identified homogeneous cell populations, thus overcoming the obstacle of tissue complexity for the analysis cell-specific expression of macromolecules. The aim of the present study was to establish the minimal conditions required for the RNA extraction and amplification from the cells captured by the laser captured microdissection. Methods : Mouse ovaries were fixed and cut into serial sections (7 im thickness). Oocytes were captured by laser captured microdissection (LCM) method by using PixCell $II^{TM}$ system. The frozen sections were fixed in 70% ethanol and stained with hematoxylin and eosin, while the paraffin sections were stained with Multiple stain. Sections were dehydrated in graded alcohols followed by xylene and air-dried for 20 min prior to LCM. All reactions were performed in ribonuclease free solutions to prevent RNA degradation. After LCM, total RNA extraction from the captured oocytes was performed using the guanidinium isothiocyanate (GITC) solution, and subsequently evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) for glyceraldehyde-3-phosphate-dehydrogenase (GAPDH). Results: With the frozen sections, detection of the GAPDH mRNA expression in the number of captured 25 oocytes were not repeatable, but the expression was always detectable from 50 oocytes. With 25 oocytes, at least 27 PCR cycles were required, whereas with 50 oocytes, 21 cycles were enough to detect GA PDH expression. Amount of the primary cDNA required for RT-PCR was reduced down to at least 0.25 $\grave{i}$ l with 50 oocytes, thus the resting 19.75 il cDNA can be used for the testing other interested gene expression. Tissue-to-slide, tissue-to-tissue forces were very high in the paraffin sections, thus the greater number of cell procurement was required than the frozen sections. Conclusion: We have described a method for analyzing gene expression at the RNA level with the homogeneously microdissected cells from the small amount of tissues with complexity. We found that LCM coupled with RT-PCR could detect housekeeping gene expression in 50 oocytes captured. This technique can be easily applied for the study of gene expression with the small amount of tissues.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.6
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• pp.927-932
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• 1998

The antioxidant activity of Symphyocladia latiuscula was determined by measuring lipid peroxide produced when a mouse liver homogenate was exposed to the air at $37^{\circ}C$, using 2-thiobarbituric acid (TBA) and radical scavenging effect on 1,1 - diphenyl - 2 - picrylhydrazyl (DPPH) radical, and free radical generation inhibition by $ACF_2$(Hepatocyte). The methanol extract of S. latiuscula showed high antioxidant activity. And the methanol extract was fractionated with several solvents. With regard their fractions, the antioxidant activity were in the order of dichloromethane > hexane > butanol > ethyl acetate > water fraction. The dichloromethane fraction showed the strongest radical scavenging activity ($50\%$ inhibitory concentration[$IC_{50}$]=3,14 $\mu$g/ml), and strong inhibitory effect on the lipid peroxidation of the mouse liver homogenate, which was compared with lascorbic acid, inhibition effect was stronger than Lascorbic acid. The methanol extract of S. latiuscula and its dichlromethane soluble fraction also inhibited over $50\%$ at concentration of 0.2 mg/ml and 0.1 mg/ml on free radical generation of hepatocyte ($AC_2F$). While the water fraction was inactive in all the assay for antioxidant activity.

• Journal of the HCI Society of Korea
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• v.2 no.2
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• pp.27-34
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• 2007

In ubiquitous computing, there is increasing demand for ubiquitous displays that react to a user's actions. We propose a method of navigating pictures on an ambient display using implicit interactions. The ambient display can identify the user and measure how far away they are using an RFID reader and ultrasonic sensors. When the user is a long way from the display, it acts as a digital picture and does not attract attention. When the user comes within an appropriate range for interaction, the display shows pictures that are related to the user and provides quasi-3D navigation using the TIP(tour into the picture) method. In addition, menus can be manipulated directly on a touch-screen or remotely using an air mouse. In an emergency, LEDs around the display flash to alert the user.

• Journal of Embryo Transfer
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• v.8 no.2
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• pp.75-82
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• 1993

To investigate the effect of EDTA on the in vitro development of blastomeres isolated from 2, 4, and 8-cell embryos(termed 1 /2, 1 /4 and 1 /8 blastomeres, respectively) of ICR strain mice, those were cultured in vitro in 35 mm culture dishes containing NaHCO$_3$-BMOC-3 medium supplemented with 10, 50, 100, or 500 $\mu$M of EDTA at 37$^{\circ}C$ for 72hrs. under the atmosphere of 5% $CO_2$and 95% air. EDTA supplementation of 10, 50, or 100 $\mu$M to medium significantly(P<0.01) increased blastocyst formation rate compared with controls in 1 /2(58.3, 63.7, and 61.3% vs 21.6%), 1 /4(54.7, 57.5 and 62.2% vs. 2L3%), and 1 /8 blastomeres(46.2, 48.7, and 57.7% vs. 19.1%). Whereas, it was significantly(P<0.01) decreased to 4.5, 2.3, and 2.0% for 1 /2, 1 /4 and 1 /8 blastomeres, respectively by the EDTA supplementation of 500 M Both the nuclear number(P<0.05) and diameter of blastocysts(P<0.01) developed from balstomeres were significantly affected by the origin of blastomeres. The nuclear number of blastocysrs developed from 1/2, 1/4, and 1/8 blastomeres ranged 28.3i1.3, 24.18i1.2, and 19.84$\pm$0.9, respectively. And the diameter of those blastocysts was 87.2$\pm$1.1, 56.4$\pm$0.9, and 39.2$\geq$0.8 $\mu$M, respectively.

• Journal of Nutrition and Health
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• v.24 no.1
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• pp.12-19
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• 1991

The chronic effects of long-term ozone exposure and dietary factors on the lipid peroxidation were investigated in mouse lung and liver tissues. Eighteen groups of mice were exposed to ozone(0.25 or 0.50 ppm) or ambient air over an 18-month period. Within each esposure regimen. animals were fed diets containing different levels of antioxidants and unsaturated fat. Ozone exposure did not have an effect on the production of thiobarbituric acid-reactive substances in lung and liver or free malondialdehyde in the liver at all levels of dietary vitamin E. An inverse relationship between the level of vitamin I supplementation and the concentration of lipid peroxidation products was observed. Results indicate the possible adaptation of animals to long-term continuous ozone exposure by unknown mechanism and the effectiveness of dietary vitamin I at sufficient level(30ppm) to protect against tissue lipid peroxidation regardless of the degree of unsaturation of the dietary fat.

• Journal of Advanced Navigation Technology
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• v.17 no.2
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• pp.240-246
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• 2013

Airtouch technology to integrate the system in the way of information delivery devices, touch screen DID this study is to develop new ways of information delivery systems. Airtouch technology to design and implement a system that can be utilized to view the college campus announcements, education, information, and employment information, and store the remote operation and sharing content, the development of cloud services to sync content via smart technology implementation fusion DID systemto develop. Packs USB interface kinek because you may be used in connection with the information appliances, and low-cost product by leveraging the Kinect sensor, Airtouch technology implementation. Types of input devices paper Airtouch technology systems, the user's hand gestures alone can interact with information appliances, smart fusion system developed by DID by tracking the user's hand movements to manipulate the mouse pointer, and information through the user's hand gestures to command the unit so that you can make. Airtouch technology smart fusion DID system technology utilizing a ripple effect on other industries, such as the online education industry, advertising, information industry increases. Also, replace the existing interface device with the versatility of a wide range of technologies, usability is an infinite expansion.

• Korean Journal of Animal Reproduction
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• v.26 no.4
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• pp.353-360
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• 2002

Although successful pronuclear formation and apposition were seen in porcine oocytes following mouse sperm injection, little is known on the morphology of male and female pronuclei following sperm injection. The objective of this study is to describe the ultrastructure of porcine zygote following murine sperm injection in relation to the chronology of pronuclear S phase. At 40h ~ 44h following in vitro maturation, Cumulus cells were removed in TCM-HEPES with 0.1% hyaluronidase. Then, spermatozoa was injected into the cytoplasm of oocytes. After. injection, all oocytes were transferred to NCSU23 medium and cultured at 39$^{\circ}C$ under 5% $CO_2$ in air. Oocytes were fixed in 2% glutaraldehyde in Dulbeccos phosphate-buffered saline and observed by Transmission Electron Microscopy. Nuclear precursor bodies were observed in each pronucleus. A cluster of large and small granules was attached in the nucleolus precursor body. After the apposition of male and female chromatin, chromatin condensation was observed throughout the nucleoplasm and nucleolus precursor bodies and condensed chromatin in contact with clusters of small and large granules and the nuclear envelope were found in apposed pronuclear regions. These results suggest that non-species specific nuclear cytoplasmic interactions take place during pronuclear formation and apposition following sperm injection.