• Journal of Crop Science and Biotechnology
• /
• 제10권3호
• /
• pp.193-200
• /
• 2007

Mungbean plants generally have a relatively close canopy, thus a large amount of self-shading can reduce yield due to poor light penetration. Modification of leaflet type can affect leaf canopy and could alter seed yield. Two multiple leaflet mutants were obtained from gamma-ray irradiation and used to study the mode of inheritance related to leaflet types and to evaluate their agronomic features. The cross between large-heptafoliate leaflet with small-pentafoliate leaflet mutants produce all $F_1$ plants with normal trifoliate leaflets. The $F_2$ plants segregated in leaflet size and leaflet number into a 9:3:3:1 ratio of large-trifoliate: large-heptafoliate: small-pentafoliate: small-heptafoliate plants, suggesting that independent loci control leaflet size and leaflet number. Regarding leaflet number, the $F_2$ population can be classified into normal-trifoliate, small-pentafoliate, large-heptafoliate, and small-heptafoliate at the dihybrid ratio of 9:3:3:1. The gene symbols $N_1,n_1$ and $N_2,n_2$ are proposed to represent leaflet number. Since no plant was found with large-pentafoliate leaflets, we hypothesize that the $N_2$ allele expresses pleiotropic effect on both leaflet number and leaflet size. Another possibility is that an additional locus with S and s alleles controls leaflet size and S is tightly linked with $N_2$. The effect of multifoliate leaflet on yield and yield components was evaluated in four mungbean families each with four leaflet isolines under three environments. Averaging across the families and environments, the normal-trifoliate and large-heptafoliate lines gave higher yield than small pentafoliate and heptafoliate ones. These two large leaflet lines also had higher leaf area per plant than the other multifoliate lines. Therefore, the mungbean lines with a greater leaf area, which were likely to intercept more sunlight, gave greater yield. Three AFLP markers that were found to be linked to number of leaflets per leaf, corresponded to the N1 allele of the smallpentafoliate parent.

• 한국육종학회지
• /
• 제40권1호
• /
• pp.39-47
• /
• 2008

1. Ds 삽입변이체 계통으로부터 제초제 저항성 1,874계통을 선발하고 농업적 주요 특성으로서 출수일수, 간장, 수수, 수장, 엽장 등 5가지 형질에 대하여 조사한 바, 조사된 5가지 형질에 대하여 원품종인 동진벼에 비하여 매우 다양한 변이폭을 보여주었다. 2. 농업적 유용성과 관련된 수장이 길고, 조기출수, 수수가 많은 변이체 뿐만 아니라 형태학적 변이를 보이는 twin seedling, dwarf, early heading, strip albino, liguleless 등 변이체가 다수 발견됨으로서 육종적 이용 및 유전자 기능해석을 위한 유용한 집단으로서 유용성을 보여주었다. 3. 서던분석 결과 벼 게놈상에서 Ds는 평균 2 copy로 전이되었으며 조직부위별로 GUS의 발현을 조사한 결과 잎, 뿌리 및 화기관등에서 약 3.9%가 발현되었다. 이 삽입변이체에서 나타난 다양한 변이형질의 주요 농업적 특성과 GUS 발현의 재현성을 위해 다음 세대의 전개를 통한 후대분석이 필요하다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.72-72
• /
• 2017

We found a new stay-green mutant from 'Pyeongwon' which is an early-maturing rice variety in Korea. The mutant showed green leaves after grain ripening period and it maintained higher SPAD value than wild type rice plant and original variety 'Pyeongwon'. The stay-green trait in rice, three genes have been identified up to date. The non-yellow coloring1 (NYC1) gene encodes a chloroplast-localized short-chain dehydrogenase/reductase (SDR) with three transmembrane domains. The non-yellow coloring3 (NYC3) gene encodes a plastid-localizing alpha/beta hydrolase-fold family protein with an esterase/lipase motif. The Sgr gene encodes a novel chloroplast protein and regulates the destabilization of the light-harvesting chlorophyll binding protein (LHCP) complexes of the thylakoid membranes, which is a prerequisite event for the degradation of chlorophylls and LHCPs during senescence. After sequencing the PCR products, we found a single nucleotide variation($A{\rightarrow}T$) in the NYC1 gene, which changes the amino acid lysine to methionine. The NYC1 gene encodes a short-chain dehydrogenase/reductase(SDR) protein. And we confirmed the co-segregation between SNP and stay-green trait from genotyping the progenies of the mutant.

• 한국작물학회지
• /
• 제24권2호
• /
• pp.9-16
• /
• 1979

1. 전형적인 조숙성 재래종의 특성은 월동전에는 잎이 담녹색 광엽 직입이며 성숙시에는 화모이 짧고 직수이며 입중이 가볍다. 또한 내한성이 약한 재내종도 상기 조숙성 품종과 같은 특성을 지니고 있다. 이는 한숙성과 약한 내한성간에는 밀접한 관계가 있기 때문에 당연한 것으로 생각된다. 2. 강한 내한성과 관련된 특성은 않지 않으나 대체로 월동전에 포복형이거나 반포복형이며 엽색은 녹색 또는 암녹색을 띠며 협엽, 장간, 당숙인 경향이 있다. 2. 강한 내한성과 관련된 특성은 많지 않으나 대체로 월동전에 포복형이거나 반포복형이며 엽색은 녹색 또는 암록색을 띠며 협엽, 장간, 중숙인 경향이 있다. 3. 조숙성 집단에서 내한성이 강한 품종의 선발은 대단히 어렵고, 또한 매년 합리적인 내한성 검정을 실시하기란 극히 곤란하나, 본 내항성과 관련된 형질연구에서 중생인 재래종을 이용하므로서 내한성이 강한 품종이 선발가능성을 보여주고 있다. 4. 중생이며 내한성이 강한 품종과 관련이 있는 것으로 여겨지는 특성은 장망, 장수, 장립, 장간, 늘어진잎, 중간지엽폭 및 장, 중간화경장, 월동전의 반포복형 및 중간엽폭 등이며, 중생이며 내한성이 약한 지엽이 넓고, 월동전에 잎이 직입, 담록색, 광엽 등의 특성이 있다.

• Mycobiology
• /
• 제46권1호
• /
• pp.72-78
• /
• 2018

The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker "SCL-18" consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.

• 한국육종학회지
• /
• 제40권4호
• /
• pp.414-421
• /
• 2008

We conducted a QTL analysis of agronomic traits using 117 $BC_3F_5$ and $BC_3F_6$ lines developed from a cross between Ilpumbyeo and Moroberekan. Genotypes of 117 $BC_3F_5$ lines were determined using 134 simple sequence repeat (SSR) markers. A total of 832 Moroberekan chromosome segments with 410 homozygous and 422 heterozygous, respectively, were detected, and the genetic distance of introgression segments ranged from 0.5 cm to 112.1 cm. A linkage map constructed using 134 SSR markers was employed to characterize quantitative trait loci (QTL). The 117 $BC_3F_5$ and $BC_3F_6$ lines were evaluated for seven agronomic traits at two locations in 2006 and 2007 and at one location in 2007. A total of 26 QTLs were identified for seven traits including days to heading, and the phenotypic variance explained by each QTL ranged from 9.2% to 24.2%. Moroberekan alleles contributed positive effects in the Ilpumbyeo background at eleven QTL loci including panicle length and spikelets per panicle. Five QTLs, two for days to heading and one each for culm length, panicle length and spikelets per panicle were consistently detected in every occasions indicating that these QTLs are stable. Among them, two QTLs, spp6 for spikelets per panicle and pl6 for paniclel length were localized in the similar region. Increase in spikelets per panicle at this locus might be due to the increase in panicle length, because both traits were associated with increase in spikelets per panicle and panicle length due to the presence of the Moroberekan allele. These Moroberekan QTLs might be useful in breeding programs to develop high-yielding cultivars.

• 한국작물학회지
• /
• 제66권2호
• /
• pp.130-137
• /
• 2021

본 실험은 지역 적응성 시험을 통한 도입 초당옥수수 자원의 농업 형질을 조사하고 혼합 선형 모형을 이용하여 조사 형질의 분산 구성요소를 추정하고 이를 통해 형질들의 유전력을 추정함으로써 신규 자원이 가진 육종재료로서의 가치를 평가하고, 이를 이용한 초당 옥수수 품종 육종 과정에서 선택할 수 있는 육종 및 선발 방법을 도모하고자 하였다. 1. 출웅기와 출사기를 제외한 모든 형질의 유전력은 낮게 추정되었는데 이러한 형질은 분산구성요소 중 유전분산이 낮게 추정되었다. 착수고율의 경우 품종과 지역 간의 유의한 상호작용효과로 인해 낮은 유전분산이 추정된 것으로 보인다. 2. 측정 형질의 낮은 유전력 추정치는 시험 재료가 모두 최신 상업용 품종이기 때문일 것으로 사료된다. 따라서 육종재료로서의 낮은 잠재력을 의미하는 것은 아니다. 3. 낮은 유전분산과 유의미한 상호작용 효과를 극복하고 선발 효율을 극대화 하기 위하여 교잡종의 직접적인 자가 수정을 통한 자식 계통 개발을 진행하기 보다 intermating을 통한 유전자 재조합을 먼저 유기하는 것이 바람직 할 것으로 보인다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.74-74
• /
• 2017

Wheat is one of the world's most important crop species. Recently, abnormal climate changes directly influence on the significant reduction of wheat productivity in the world. This threaten allow breeders to find new genetic resources. Wheat is one of the widely grown crops in the world. Individual cultivars / germplasm is adapted in that region where the climate is unique to each other. Therefore, introducing new genetic resources which was good in one place may better perform in another region. In this study, we evaluated germplasm in Korean environment and measured numerous agro-morphological characteristics. Information that are provided by the National Agrobiodiversity Center (Jeonju, Korea) and National Plant Germplasm System (Aberdeen, USA) were included in the analysis. Cluster analysis was performed using the unweight pair-group method of averages. The results of PCA indicated principal discriminatory characteristics of wheat landraces and varieties. Significant differences indicated high variability among the quantitative traits. Cluster analysis results showed that the groups were divided by geological climate condition. The preliminary evaluation of germplasms in Korean environment would help to develop wheat cultivars via providing useful genetic traits that are resided in alien germplasms.

• Journal of Plant Biotechnology
• /
• 제45권1호
• /
• pp.17-29
• /
• 2018

본 연구에서는 국내외에서 수집한 벼 294개 유전자원 핵심집단을 대상으로 벼의 지엽각 특성에 대한 조사를 수행하였고, GWAS를 이용하여 지엽각 연관 유전자를 추출 및 분석하였다. 표현형 데이터를 이용한 GWAS의 Manhattan plot 결과 분석을 통해, 각 집단에서 염색체를 대상으로 표현형과 통계적 유의성을 나타내 연관성을 보이는 SNP를 발굴하였다. 지엽각 관련 특성에 대하여 선행 연구된 QTL region과의 비교를 통하여 본 연구에서 발굴된 SNP간의 유의성을 조사한 결과, 지엽각과 유의성이 있는 SNP (S8-19815442)가 이미 확인된 QTL region에 위치하는 것으로 나타났으며, 후보유전자 Os08g31950 대해 연관 유전자 변이를 관찰하기 위해서 형질 특이적 품종군 간의 염기서열을 비교한 결과 1개의 지역에서 단일염기변이가 검출되었다. Os08g31950의 조직별 RNA의 상대적 발현량 수준을 비교한 결과, Os08g31950 유전자는 모든 조직에서 높은 발현량을 확인할 수 있었으며 조직별로 다양한 발현 양상을 관찰할 수 있었다. 또한, 모두 직립형 품종군에서 상대적으로 발현량이 높게 나타났으며 뿌리보다 잎에서의 발현율이 높게 나타났다. 본 연구를 통해 동정된 지엽각 연관 후보유전자 Os08g31950는 벼 생육 및 수량 증대에 이용할 수 있는 마커제작 및 육종의 기초자료가 될 것으로 기대된다.

• 한국작물학회지
• /
• 제64권3호
• /
• pp.246-268
• /
• 2019

Soybean [Glycine max (L.) Merr.] is a species of legume native to East Asia. The interactions between climatic conditions and genetic characteristics are known to affect the agricultural performance of soybean. Therefore, the present investigation was conducted to identify the main elements affecting the agricultural performances of 11 soybean varieties/lines from China [Harbin ($45^{\circ}12^{\prime}N$), Yanji ($42^{\circ}53^{\prime}N$), Dalian ($39^{\circ}30^{\prime}N$), Qingdao ($36^{\circ}26^{\prime}N$)] and the Republic of Korea [Suwon ($37^{\circ}16^{\prime}N$), and Jeonju ($35^{\circ}49^{\prime}N$)]. The days to flowering (DTF) of soybeans with the e1-nf and e1-as alleles and the E1e2e3e4 genotype, except in 'Keumgangkong', 'Tawonkong', and 'Duyoukong', were relatively short compared to those of soybeans with other alleles. Although DTF of the soybeans was highly correlated with all climatic conditions [negative: precipitation, average temperature (AVT), accumulated temperature; positive: day-length (DL)], days to maturity and 100-seed weight of the soybeans showed no significant correlation with any climatic conditions. The soybeans with a dominant Dt1 allele, except 'Tawonkong', had the longest stem length (STL). Moreover, STL of the soybeans grown in the test fields showed a positive correlation with only DL; however, the results of our chamber test that was conducted to complement the field tests showed that STL of soybean was positively affected by AVT and DL. Although soybean yield (YLD) showed positive correlations with latitude and DL (except L62-667, OT89-5, and OT89-6), the response of YLD to the climatic conditions was cultivar-specific. Our results show that DTF and STL of soybeans grown in six different latitudes are highly affected by DL, and AVT and genetic characteristic also affect DTF and STL.