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http://dx.doi.org/10.1080/12298093.2018.1454004

Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae  

Yao, Fang-Jie (College of Horticulture, Jilin Agricultural University)
Lu, Li-Xin (Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University)
Wang, Peng (Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University)
Fang, Ming (College of Horticulture, Jilin Agricultural University)
Zhang, You-Min (College of Horticulture, Jilin Agricultural University)
Chen, Ying (Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University)
Zhang, Wei-Tong (Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University)
Kong, Xiang-Hui (Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University)
Lu, Jia (College of Horticulture, Jilin Agricultural University)
Honda, Yoichi (Graduate School of Agriculture, Kyoto University)
Publication Information
Mycobiology / v.46, no.1, 2018 , pp. 72-78 More about this Journal
Abstract
The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker "SCL-18" consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.
Keywords
Auricularia auricula-judae; fruiting body pattern; sequence-related amplified polymorphism; sequence characterized amplified region;
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