• The Plant Pathology Journal
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• v.36 no.4
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• pp.323-334
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• 2020

Lysin motif (LysM) proteins are reported to be necessary for the virulence and immune response suppression in many herbaceous plant pathogens, while far less is documented in woody plant pathogens. In this study, we preliminarily characterized the molecular function of a LysM protein LtLysM1 in woody plant pathogen Lasiodiplodia theobromae. Transcriptional profiles revealed that LtLysM1 is highly expressed at infectious stages, especially at 36 and 48 hours post inoculation. Amino acid sequence analyses revealed that LtLysM1 was a putative glycoprotein with 10 predicted N-glycosylation sites and one LysM domain. Pathogenicity tests showed that overexpressed transformants of LtLysM1 displayed increased virulence on grapevine shoots in comparison with that of wild type CSS-01s, and RNAi transformants of LtLysM1 exhibited significantly decreased lesion length when compared with that of wild type CSS-01s. Moreover, LtLysM1 was confirmed to be a secreted protein by a yeast signal peptide trap assay. Transient expression in Nicotiana benthamiana together with protein immunoblotting confirmed that LtLysM1 was an N-glycosylated protein. In contrast to previously reported LysM protein Slp1 and OsCEBiP, LtLysM1 molecule did not interact with itself based on yeast two hybrid and co-immunoprecipitation assays. These results indicate that LtLysM1 is a secreted protein and functions as a critical virulence factor during the disease symptom development in woody plants.

• Genes and Genomics
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• v.40 no.11
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• pp.1181-1197
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• 2018

Tropical plant rubber tree (Hevea brasiliensis) is the sole source of commercial natural rubber and low-temperature stress is the most important limiting factor for its cultivation. To characterize the gene expression profiles of H. brasiliensis under the cold stress and discover the key cold stress-induced genes. Three cDNA libraries, CT (control), LT2 (cold treatment at $4^{\circ}C$ for 2 h) and LT24 (cold treatment at $4^{\circ}C$ for 24 h) were constructed for RNA sequencing (RNA-Seq) and gene expression profiling. Quantitative real time PCR (qRT-PCR) was conducted to validate the RNA-Seq and gene differentially expression results. A total of 1457 and 2328 differentially expressed genes (DEGs) in LT2 and LT24 compared with CT were respectively detected. Most significantly enriched KEGG pathways included flavonoid biosynthesis, phenylpropanoid biosynthesis, plant hormone signal transduction, cutin, suberine and wax biosynthesis, Pentose and glucuronate interconversions, phenylalanine metabolism and starch and sucrose metabolism. A total of 239 transcription factors (TFs) were differentially expressed following 2 h or/and 24 h of cold treatment. Cold-response transcription factor families included ARR-B, B3, BES1, bHLH, C2H, CO-like, Dof, ERF, FAR1, G2-like, GRAS, GRF, HD-ZIP, HSF, LBD, MIKC-MADS, M-type MADS, MYB, MYB-related, NAC, RAV, SRS, TALE, TCP, Trihelix, WOX, WRKY, YABBY and ZF-HD. The genome-wide transcriptional response of rubber tree to the cold treatments were determined and a large number of DEGs were characterized including 239 transcription factors, providing important clues for further elucidation of the mechanisms of cold stress responses in rubber tree.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.1
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• pp.73-79
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• 2016

The objective was to evaluate the effect of fodder tree species (FTS) with condensed tannin contents: Cordia elaeagnoides, Platymiscium lasiocarpum, Vitex mollis, and Haematoxylon brasiletto, on in vitro methane ($CH_4$) production at 24 h post incubation. The analysis was performed using the in vitro gas production technique, with three levels of inclusion/species: 600, 800, and 1,000 mg and with 4 replicates/species/level of inclusion. The substrate was incubated at $39^{\circ}C$, and the gas and $CH_4$ production were recorded at 4, 8, 12, and 24 h post incubation. The data collected was analyzed through Pearson correlation, polinomial regression and fixed effects models. There were negative correlations between FTS-total gas volume (r = -0.40; p<0.001); FTS-volume of $CH_4$ produced (r = -0.40; p<0.001) and between the inclusion level-volume of $CH_4$ produced (r = -0.20; p<0.001). As well as a positive correlation between hours post incubation-total gas volume (r = 0.42; p<0.001) and between hours post incubation-volume of $CH_4$ produced (r = 0.48; p<0.001). The FTS: C. elaeagnoides, V. mollis, and H. brasiletto have potential, in the three inclusion levels analyzed, to reduce $CH_4$ emission on in vitro trials (>32.7%), taking into account the total $CH_4$ production at 24 h of the forage used as reference (Avena sativa). It's suggested that C. elaeagnoides-according to its crude protein, neutral detergent fiber, and condensed tannins content- is the best alternative within the FTS analyzed, for feeding ruminants and for the control of $CH_4$ emissions during the dry season.

• Journal of Practical Agriculture & Fisheries Research
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• v.26 no.1
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• pp.30-39
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• 2024

Korean arboretums where a large number of people are visiting could not do visitor's satisfaction in qualitative side and communication through education and event, although a lot of those things statistically have been doing so far. At this side, it needs an arboretum's segmentation: preparing to an adaptation strategy of a new paradigm, differentiated strategy, and problem solving strategy tied by a similar group for the Korean arboretum visitors. This research tries to classify several patterns with Korean arboretums by visitor's behavior and propose each type of subspecialized management plan after analyzing characteristic of supply and demand. The research for this tries to classify several patterns of Korean arboretum and suggest segmentalzed management plan by types after analyzing characteristics of aspects of demand and supply. By looking at the main patterning result of arboretum as a standard of the demand, there were four sorts of arboretums. In the first, it is the arboretum of intermediate type: not a clear characteristic. In the second, it is an arboretum of multipurpose resort type: strongly demanding motivational factors of a rest, health, access, low cost, experience, learning and sightseeing. In the third, it is an arboretum of theme tour type: a strong sightseeing motivational factor and demanding appreciate scenery. In the last, it is an arboretum of studying and admiring type: demanding a motivational factor of sightseeing landscape and plants, a strong experience and learning.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.9
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• pp.1237-1247
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• 2012

The objective of this study was to evaluate the predictions of dry matter intake (DMI) and average daily gain (ADG) of Vietnamese Yellow (Vang) purebred and crossbred (Vang with Red Sindhi or Brahman) bulls fed under Vietnamese conditions using two levels of solution (1 and 2) of the large ruminant nutrition system (LRNS) model. Animal information and feed chemical characterization were obtained from five studies. The initial mean body weight (BW) of the animals was 186, with standard deviation ${\pm}33.2$ kg. Animals were fed ad libitum commonly available feedstuffs, including cassava powder, corn grain, Napier grass, rice straw and bran, and minerals and vitamins, for 50 to 80 d. Adequacy of the predictions was assessed with the Model Evaluation System using the root of mean square error of prediction (RMSEP), accuracy (Cb), coefficient of determination ($r^2$), and mean bias (MB). When all treatment means were used, both levels of solution predicted DMI similarly with low precision ($r^2$ of 0.389 and 0.45 for level 1 and 2, respectively) and medium accuracy (Cb of 0.827 and 0.859, respectively). The LRNS clearly over-predicted the intake of one study. When this study was removed from the comparison, the precision and accuracy considerably increased for the level 1 solution. Metabolisable protein was limiting ADG for more than 68% of the treatment averages. Both levels differed regarding precision and accuracy. While level 1 solution had the least MB compared with level 2 (0.058 and 0.159 kg/d, respectively), the precision was greater for level 2 than level 1 (0.89 and 0.70, respectively). The accuracy (Cb) was similar between level 1 and level 2 (p = 0.8997; 0.977 and 0.871, respectively). The RMSEP indicated that both levels were on average under-or over-predicted by about 190 g/d, suggesting that even though the accuracy (Cb) was greater for level 1 compared to level 2, both levels are likely to wrongly predict ADG by the same amount. Our analyses indicated that the level 1 solution can predict DMI reasonably well for this type of animal, but it was not entirely clear if animals consumed at their voluntary intake and/or if the roughness of the diet decreased DMI. A deficit of ruminally-undegradable protein and/or a lack of microbial protein may have limited the performance of these animals. Based on these evaluations, the LRNS level 1 solution may be an alternative to predict animal performance when, under specific circumstances, the fractional degradation rates of the carbohydrate and protein fractions are not known.

• Journal of the Korea Furniture Society
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• v.19 no.1
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• pp.75-82
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• 2008

A series of the studies on the applied physical properties of main domestic species have been conducted last three years. Equilibrium moisture content(EMC) of Pinus koraiensis, Larix kaemferi, Pinus koraiensis were investigated. The experiments for sorption property were conducted with 20- and 80-mesh wood powder and resulted in their EMC's at various sorption conditions. Amount of moisture sorption and sorption speed of Larix kaemferi were shown the most large values and very fast among three Korean main coniferous wood.

• The Korean Journal of Soil Zoology
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• v.1 no.2
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• pp.95-101
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• 1996

A total of 101 species belonging to 76 genera under 42 families in Kwangreung coniferous forest (45th compartment) and 108 species belonging to 69 genera under 40 families in Kwangreung deciduous forest (44th compartment) was reported, respectively.

• Communications for Statistical Applications and Methods
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• v.10 no.1
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• pp.39-48
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• 2003

Quality in agriculture statistics at the international level is very important to all countries in the world. We investigate international organizations which produce worldwide agricultural statistics. The UN-FAO is one of the most important organization in agricultural statistics fields. We first introduce the Statistics division in FAO and present some considerations like production, reliability, quality of the agriculture data from my own experience as a consultant in FAO and Ministry of Agriculture and Forestry in Korea.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.5
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• pp.690-697
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• 2012

Approximately 67% of the total land area of the Korea is covered by forest. Eutrophication, defined as the enrichment of waters beyond natural levels, principally by the nutrient phosphorus (P), is a serious cause of concern at the present time. The contribution of forestry to P loading in catchment waters has not been intensively studied in Korea, but is potentially important because forests are often located in near-pristine environments. Phosphorus is retained by most mineral soils and, as a consequence, losses are usually negligible. However, it is much more mobile in organic soils where it can be relatively easily leached or lost through surface runoff, as these soils have a low capacity to retain free phosphate. This report has been prepared to study the influence of arable land used for paddy, upland, and forestry on water quality in the basin of Daechung reservoir.

• Journal of the Korean Society for Library and Information Science
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• v.29
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• pp.205-230
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• 1995

The publication of books on modern agriculture started under the influence of Silhak Thought developed from the late Chosun Dynasty. The common intention found in these publications is the objective to enrich the country through increased agricultural productivity and thus to secure national independence from the surrounding powers. The study reviews three different categories in this area; five general books on agriculture, three on agriculture related legal regulations, and ten books introducing techniques of commercial agriculture. The first category is comprehensive treatment of general agricultural content and the second is legal regulations which affected the contemporary agriculture. The third category is introductory books on commercial techniques aimed at accumulating wealth through agriculture. Silkworm cultivation occupies an important place in these publications owing to the active encouragement given by the Section of Silkworm Cultivation in the Ministry of Agriculture, Commerce and Industry. We can recognise the extensive involvement of Suh Pyung Sook, who served as the Section Chief of Silkworm Cultivation in 1904, by examining introductions and prefaces of many books in this category. The examples of general books on agriculture are 'New Agricultural Administration' (농정신편), 'General Introduction on Agiculture' (농업대요), 'New Textbook on Agricuture' (신찬농업교과서), 'Pragmatic Agriculture' (실리농방신편), Of these, 'New Agricultural Administration' (농정신편) is evaluated as the first publication on modern agriculture. It was written in 1881, and the first edition was published was published in 1901 with the second edition following in 1905. Examples of the second category are; 'The regulation on utilizing uncultivated state owned land' (국유미간지리용법) legislated and declared with the intention of expanding cultivated area, 'Mining and Forestry Regulation' (광임법규), 'Forest Land Regulation' (임야법령) to administer mining and forestry. Books on commercial agriculture take up the highest proportion in the agricultural publication of this period. These cover silkworm cultivation, chicken farming, vegetables and fruits. The books introduce Western techniques with more scientific and rational approach especially on Silkworm cultivation and chichen farming which had become increasingly significant as commercial agriculture from the late Chosun Dynasty.