• 식물병연구
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• 제16권3호
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• pp.306-311
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• 2010

2009년 국내산 옥수수 19점과 벼 32점을 대상으로 Fusarium 오염 및 Fusarium 독소 오염을 조사하였다. 옥수수와 벼 시료의 Fusarium 오염률은 각각 54.9%와 8.2%로 확인되었으며, 종 특이 primer를 이용한 PCR 증폭결과 옥수수시료에서 분리된 506균주 중 58균주의 F.graminearum 추정균주(11.5%)와 354균주의 F. verticillioides 추정균주(70.0%)가 동정되었다. 또한 벼의 경우, 분리된 315균주 중 276균주(87.8%)가 F. graminearum으로 추정 되었으며, F. verticillioides 추정균주는 검출되지 않았다. LC 및 LC-MS를 이용한 Fusarium 독소(DON, NIV, ZEA, FB)의 자연발생량 조사 결과, DON과 ZEA이 각각 2개의 옥수수 시료에서만 기준치 이상 검출되었다. FB는 대부분의 옥수수 시료와 한 개의 벼 시료에서 검출 되었으나 모두 기준치 이하였다. 따라서 본 연구에서 사용된 2009년산 옥수수와 벼의 곰팡이독소 오염수준은 대부분 기준치 이하로 심각하지 않았다.

• The Plant Pathology Journal
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• 제33권5호
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• pp.499-507
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• 2017

In an attempt to develop a biological control agent against mycotoxigenic Fusarium species, we isolated Bacillus amyloliquefaciens strain DA12 from soil and explored its antimicrobial activities. DA12 was active against the growth of mycotoxigenic F. asiaticum, F. graminearum, F. proliferatum, and F. verticillioides both in vitro and in planta (maize). Further screening using dual culture extended the activity range of strain DA12 against other fungal pathogens including Botrytis cinerea, Colletotrichum coccodes, Endothia parasitica, Fusarium oxysporum, Raffaelea quercus-mongolicae, and Rhizoctonia solani. The butanol extract of the culture filtrate of B. amyloliquefaciens DA12 highly inhibited the germination of F. graminearum macroconidia with inhibition rate 83% at a concentration of $31.3{\mu}g/ml$ and 100% at a concentration of $250{\mu}g/ml$. The antifungal metabolite from the butanol extract was identified as iturin A by thin layer chromatography-bioautography. In addition, volatile organic compounds produced by DA12 were able to inhibit mycelial growth of various phytopathogenic fungi. The volatile compounds were identified as 2-heptanone, 5-methyl heptanone and 6-methyl heptanone by gas chromatography-mass spectrometry (GC-MS) analysis. These results indicate that the antagonistic activity of Bacillus amyloliquefaciens DA12 was attributable to iturin A and volatile heptanones, and the strain could be used as a biocontrol agent to reduce the development of Fusarium diseases and mycotoxin contamination of crops.

• 한국식품위생안전성학회지
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• 제29권3호
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• pp.181-188
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• 2014

본 논문은 황기재배 농가의 미생물학적 위해요소를 분석하였다. 충북 제천소재의 4곳의 농가를 선정하여 수확 후 처리 설비 (세척기, 세척수, 손수레, 선별 작업대, 건조선반, 결속기), 작업자의 손 및 수확 후 처리 단계별 황기(세척 전, 세척 후, 선별 후, 건조 후)로부터 샘플을 채취하여 위생지표세균과 병원성 미생물에 대한 오염도를 조사하였다. 수확 후 처리 설비의 총 호기성 세균과 대장균 군은 각각 0.93~4.86, 0.33~2.28 log CFU/$100cm^2$, mL의 수준으로 검출되었으며, 작업자의 손에 대한 미생물의 오염도는 5.43~6.11, 2.52~4.12 log CFU/Hand로 수확 후 처리설비보다 높게 검출되었다. 병원성 미생물에 대한 연구결과, B. cereus가 수확 후 처리 설비, 작업자의 손, 황기에서 각각 0.33~2.41 log CFU/$100cm^2$, mL, 1.48~3.27 log CFU/Hand, 0.67~3.65 log CFU/g으로 측정되었다. 정량분석에서는 S. aureus가 측정되지 않았지만, 정성분석에 의해 세척기, 작업대, 작업자의 손, 황기 (세척 전, 선별 후)에서 검출되었다. 본 연구에서 황기의 수확 후 처리 설비와 황기에서의 미생물학적 오염도를 측정한 결과, 개인 위생 및 작업시설의 위생상태를 청결하게 유지하여 교차오염을 방지해야 할 필요가 있는 것으로 판단된다.

• 한국정보통신학회논문지
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• 제16권5호
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• pp.1071-1076
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• 2012

안전농산물에 대한 관심이 많아진 요즘, 농산물의 안전성을 위한 시도가 활발히 이루어지고 있다. 그 중 하나가 농산물우수관리인증(GAP) 정보 서비스이다. 농산물우수관리인증 정보 서비스는 농산물의 생산단계에서 판매단계까지의 농산식품 안전관리체계를 구축하여 소비자에게 안전한 농산물을 공급하는 제도이다. 본 논문에서 제시하는 시스템은 사용자가 쉽게 사용 할 수 있도록 RFID(Radio Frequency Identification)를 이용하여 보다 쉽게 GAP 정보 서비스를 제공한다.

• 한국안전학회지
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• 제38권2호
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• pp.60-67
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• 2023

This study identified hazard exposures, health problems, and job satisfaction among elderly agricultural workers. A total of 1,340 agricultural workers (1,079 males and 261 females) were extracted from the 6^th Korean Working Conditions Survey data. Three age groups (A: 40s or below, B: 50s, and C: 60s or above) were statistically compared in hazard exposures, health problems, and job satisfaction. Elderly agricultural workers (60s or above) had significantly more exposure to ergonomic hazards, severe health problems, and lower job satisfaction than young agricultural workers. The study findings may help understand the characteristics of hazard exposures, health problems, and job satisfaction of elderly agricultural workers and will be useful for improving these conditions.

• 한국농공학회논문집
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• 제53권2호
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• pp.9-17
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• 2011

Based on the statistical annual report, there are 17,649 reservoirs are operating for the purpose of agricultural water supply in Korea. 58 % of entire agricultural reservoirs had been constructed before 1948 which indicate the termination of required service life and rest of those reservoirs have also exposed to the dam break risk by extreme flood event caused by current ongoing climate change. To prevent damages from dam failure accident of these risky small size dams, it is necessary to evaluate and manage the structural and hydrological safety of the reservoirs. In this study, a simplified evaluation method for hydrologic safety of dam is suggested by using Rational and Creager formula. Hydrologic safety of small scale dams has evaluated by calculating flood discharge capacity of the spillway and compares the results with design frequency of each reservoir. Applicability and stability of suggested simplified method have examined and reviewd by comparing the results from rainfall-runoff modeling with dam break simulation using HEC-HMS. Application results of developed methodology for three sample reservoirs show that simplified assessment method tends to calculate greater inflow to the reservoirs then HEC-HMS model which lead lowered hydrologic safety of reservoirs. Based on the results of application, it is expected that the developed methodology can be adapted as useful tool for small scale reservoir's hydrologic safety evaluation.

• 드라이브 ㆍ 컨트롤
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• 제20권3호
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• pp.42-49
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• 2023

In this study, the safety of fastening device for the agricultural by-product collector was evaluated according to the driving ground conditions by deriving the stress, static safety factor, and fatigue life using dynamic simulation. A 3D modeling of agricultural by-product collector was carried out, and simulation model was developed by applying the material properties. As a result of dynamic simulation, the magnitude of the maximum stress generated in the fastening device was the highest when driving on the flat off-road, followed by sloped pave-road and flat pave-road. Static safety factor and fatigue life were the highest when driving on the flat pave-road, followed by sloped pave-road and flat off-road. The safety of fastening device was confirmed that static safety factor was more than 1.0 and service life exceeded 9 years in all driving ground conditions.

• 한국농공학회논문집
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• 제60권1호
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• pp.121-131
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• 2018

Past climate change influences multiple environmental aspects, certain of which are specifically related to agricultural water resources such as water supply and demand. Changes on rainfall and hydrologic patterns can increases the occurrence of reservoir water shortage and affect the future availability of agricultural water resources. It is a main concern for sustainable development in agricultural water resources management to evaluate adaptation capability of water supply under the changing climate and farming methods in paddy field. The purpose of this study is an evaluation method of design frequency of drought and water supply safety for agricultural reservoirs to investigate evidence of climate change occurrences at a local scale. Thus, it is a recommended practice in the development of water supply management strategies on reservoir operation under changing climate and farming methods in paddy field.

• 한국산업보건학회지
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• 제31권4호
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• pp.385-395
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• 2021

Objectives: This study aimed to select items for technical guidelines through the guidelines of the Korean Occupational Safety and Health Agency (KOSHA) for agricultural workplaces. Methods: All 1,308 guidelines provided by KOSHA were reviewed and categorized using a Delphi technique questionnaire on their compatibility and urgency. Results: Among all the KOSHA guidelines, 100 items related to agricultural workplaces were selected. After that, two Delphi questionnaires were conducted and 46 items were finally selected. The average compatibility was calculated as 4.26, and urgency was 2.39. As a result of measuring the content validity of 46 items, six items were identified that were not relevant to agriculture. The final selected items were classified into four categories: Health examination and management, machinery standards, safety and health standard guides, and workplace environment management. Conclusions: The various risk factors at agricultural workplaces should be prevented and managed. It was shown that related technical guidelines or work standard manuals should be prepared. The technical guidelines of KOSHA will be provided as basic categories in the agricultural sector.

• 한국농공학회논문집
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• 제57권5호
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• pp.123-128
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• 2015

This study conducted a series of field experiments using soil conditioners, Polyacrylamide(PAM) and gypsum, to evaluate their effects in reducing sediment loss and surface runoff. In addition, the correction factors (K-alpha) for the erodibility factor (K) were determined to reflect the effects of PAM and PAM+gypsum in applying the USLE equation. Experimental erosion plots individually sized $10m^2$ (5 m long, 2 m wide and 1 m deep) have different slopes (10, 20 and 30%). Erosion plots were prepared for one control (C; no PAM and gypsum) and two treatments (P; PAM 20 kg/ha, PG; PAM 20 kg/ha+gypsum 3,000 kg/ha). The amounts of soil eroded and runoff were continuously monitored from July $1^{st}$ to Oct. $31^{st}$ in 2010 and compared to each other. The amount of sediment loss from a control plot was 399.2 ton/ha and the relative reduction of sediment loss were 11.4% and 33.4% for PAM-treated and PAM+gypsum treated plots, respectively. This study also determined the K-alpha factors in the USLE equation to account for the erosion control effectiveness of PAM and gypsum application. The K-alpha factors were calculated as 0.92 for PAM-treated plot and 0.69 for PAM+gypsum-treated plot. The findings of this study revealed that soil conditioners (PAM and gypsum) could play a significant role in controlling soil erosion. In addition, the modified USLE equation using the K-alpha could provide valuable information to make better decision on establishment of best management practice for soil erosion control in agriculture.