• Journal of the Korean Institute of Landscape Architecture
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• v.32 no.4
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• pp.49-58
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• 2004

This study is aimed to define and classify GREEN TOURISM (GT) resources as the basis for rural sustainable development, and to analyze the current conditions of GT and applicable resources in Korea. For these purposes, a field survey about 24 rural villages in Japan and Korea is applied, accompanied by a questionnaire survey of 30 specialists, as well as a literature review. The results of this study are summarized as follows. The concept of GT has 3 important points: interchange between rural and urban residents, staying in villages, and residents' participation. GT resource are divided into 4 types: Agricultural, Ecological, Historical and Living resources in a space and activity program. In addition, the GT resources in Japan are more quantitatively abundant than in Korea, but Korean agricultural and ecological resources were more abundant relatively. According to the several methods above, 18 space and 14 activity programs were classified. Lastly, this study analyzed the applicable 32 Korean resources using the specialists' questionnaire survey. Among the space resources, ecological landscape, farm produce, farm-land, water, and historical landscape have higher applicability, but the applicability of sports, event, commemoration, and divine space are lower. Among the activity programs, farm produce, animal, plant, food&beverage, and living appliance have higher applicability.

• Journal of Korean Society of Rural Planning
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• v.4 no.1
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• pp.40-55
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• 1998

The objectives of this study are to 1)research the importance of direct trade of agricultural products between farmers and consummers, 2)invest the trade process of agricultural products, 3)survey the patterns of direct trade of agricultural products, 4)find out the situations of direct trade of agricultural products, 5)suggest the directions to improve the direct trade of agricultural products. The data were collected from Taegu city, Taechun city and Kyungsang poop do for this study. The major findings of this study to improve the direct trade of agricultural products between urban and rural are as follows; First, it is very important that producers and consummers have common sense to the direct trade between urban and rural. Second, local government related the trade of agricultural products should positively take parts in the direct trade of agricltural products between producers and consummers in administration and finance. Thirth, non-profit organizations above other organizations in urban and rural must lead the direct trade of agricultural products between urban and rural. Fourth, the markets for direct trade of agricultural products are to be opened according to basing on consummers'needs to agricultural products. Fifth, the engaged farmings between producers and consummers are to be more positive by significant relationship( promised brothers and sisters) between rural and urban. Sixth, people who moved to urban from rural should be finked out in each community(in rural), they can buy the agricultural products in the community that they were born. Seventh, The communities in rural must produce the specific agricultural products in each community.

• Journal of Microbiology and Biotechnology
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• v.24 no.10
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• pp.1421-1426
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• 2014

Although a large number of AroA enzymes (EPSPS: 5-enopyruvylshikimate-3-phosphate synthase) have been identified, cloned, and tested for glyphosate resistance, only two AroA variants, derived from Agrobacterium tumefaciens strain CP4 and Zea mays, have been utilized to produce the commercial glyphosate-resistant crops. Here, we have used a PCR-based twostep DNA synthesis method to synthesize an aroA gene ($aroA_{A.\;metalliredigens}$) from Alkaliphilus metalliredigens, encoding a new EPSPS. Furthermore, transgenic Arabidopsis with the new $aroA_{A.\;metalliredigens}$ gene was obtained to confirm the potential of the novel aroA gene in developing glyphosate-resistant crops.

• Journal of Plant Biotechnology
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• v.41 no.3
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• pp.107-115
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• 2014

Vegetable oils (triacylglycerols) produced mainly in seeds of plants are used for valuable foods that supply essential fatty acids for humans as well as industrial raw materials and biofuel production. As the demanding for vegetable oils has increased, plant metabolic engineering to produce triacylglycerols in biomass such as leaves has been considered and explored for alternative source of vegetable oils. Leaves are genetically programmed to supply the fixed carbon by photosynthesis to other organs for plant development and growth. Therefore, in order to produce and accumulate triacylglycerols in leaves, one should take account of multiple metabolic pathways such as carbon flux, competition of carbohydrate and fatty acid biosynthesis, and triacylglycerols turnover in leaves. The recent metabolic engineering strategy has showed potential in which the co-expression of three genes WRINKLED1, DGAT1, and OLEOSIN involved in the critical step for increasing the fatty acid synthesis, accumulating triacylglycerols, and protecting triacylglycerols, respectively produced higher amount of vegetable oils in leaves. Developing of genetically engineered plants producing vegetable oil in biomass at non-agricultural lands will be promising to the future success of the field.

• Korean Journal of Environmental Agriculture
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• v.40 no.4
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• pp.248-259
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• 2021

BACKGROUND: Contaminated water was a major source of food-borne pathogens in various recent fresh produce-related outbreaks. This study was conducted to investigate the microbial contamination level and correlations between the level of sanitary indicator bacteria and the detection ratio of pathogens in agricultural water by logistic regression analysis. METHODS AND RESULTS: Agricultural water was collected from 457 sites including surface water (n=300 sites) and groundwater (n=157 sites) in South Korea from 2018 to 2020. Sanitary indicator bacteria (total coliform, fecal coliform, and Escherichia coli) and food-borne pathogens (pathogenic E. coli, E. coli O157:H7, Salmonella spp., and Listeria monocytogenes) were analyzed. In surface water, the coliform, fecal coliform, and E. coli were 3.27±0.89 log CFU/100 mL, 1.90±1.19 log CFU/100 mL, and 1.39±1.26 log CFU/100 mL, respectively. For groundwater, three kinds of sanitary indicators ranged in the level from 0.09 - 0.57 log CFU/100 mL. Pathogenic E. coli, Salmonella and Listeria monocytogenes were detected from 3%-site, 1.5%- site, and 0.6%-site water samples, respectively. According to the results of correlations between the level of sanitary indicator bacteria and the detection ratio of pathogens by logistic regression analysis, the probability of pathogen detection increased individually by 1.45 and 1.34 times as each total coliform and E. coli concentration increased by 1 log CFU/100mL. The accuracy of the model was 70.4%, and sensitivity and specificity were 81.5% and 51.7%, respectively. CONCLUSION(S): The results indicate the need to manage the microbial risk of agricultural water to enhance the safety of fresh produce. In addition, logistic regression analysis is useful to analyze the correlation between the level of sanitary indicator bacteria and the detection ratio of pathogens in agricultural water.

• Food Science and Preservation
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• v.14 no.6
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• pp.695-701
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• 2007

This study was carried out to develop a standard methods of mechanized salt-inserting salting process for winter cabbage to produce kimchi. Mid or large scaled mechanized kimchi plants were surveyed to select dominant variables of the mechanized salting process. Salt concentration of salting water, salting time and dewatering time were selected as the dominant variables of the salting process. A series of laboratorial experiment was performed with winter cabbage to determine desirable conditions for the variables. A supplementary experiment with cabbages of the other seasons was executed also to compare the determined conditions each other. Finally the determined conditions of the salting process were tested by plant scale experiments with winter cabbage and proved their validity.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.10a
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• pp.118-119
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• 2003

Baculovirus occlusion bodies have been recently engineered to incorporate foreign protein such as the Bacillus thuringiensis (Bt) CrylAc protein for improvement of insecticidal activity. In this study, polyhedrin, cylAc, egfp and crylCa genes were fused to produce occlusion bodies that contain novel recombinant crystal protein by homologous recombination between cylAc and crylCa genes in insect cells. (omitted)

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.134.2-135
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• 2003

The plant-pathogenic species S. scabies, S. acidiscabies, and S. turgidiscabies cause the scab disease of potato and produce the phytotoxins, thaxtomins. necl, a gene conferring a necrogenic phenotype, is involved in pathogenicity and physically linked to the thaxtomin A biosynthetic genes. Identification of the pathogenic strains of Streptomyces from soil was performed through the polymerase chain reaction by using specific pathogenicity primer sets derived from the necl gene sequences of Streptomyces smbies. The DNA was extracted from soil using a bead-beating machine and modifications of the FastPrep system. The DNA was suitable for direct use in the PCR. The PCR products showed the bands of approximately 460 bp. This methods can be very usuful in identifying species responsible for scab diseases and studying on the ecology of plant-pathogenic Streptomyces spp.

• Journal of Agricultural Extension & Community Development
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• v.19 no.3
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• pp.729-755
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• 2012

The role of branding has been to differentiate products, but brands have been increasingly applied to organizational image too. The brand image holds a key position by the marketing system in this era. The main purpose of this research is to analyze the case for the development of farm brand. A in-depth interview was conducted to analyze the case for development of farm brand in connection with agricultural products and their business operation. The farms used various stories in the process of develop brand including farm history, cultivation process, product, management, relationship with consumers. The study present the findings divide into brand naming, image, positioning. The farms built trust by consumer relationship with the farm brand. The results of the study provide insights for agricultural marketers who intend to design brand stories that align with their produce.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.2-11
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• 2020

Assisted reproductive technologies (ART) merely depend on improving the oocyte maturation and their developmental competence to produce good quality embryos. Oocyte maturation passes through long and complex molecular steps starts from the early embryonic life and ends with sperm fertilization. Oocyte developmental competence can be attained by improving the nuclear and cytoplasmic mechanisms together with some epigenetic maturation. In this review, we highlight the cornerstones of oocyte maturation on both nuclear and cytoplasmic levels. Interfering or supporting these molecular mechanisms would help in the development of novel regulating agents for reproductive performance of humans and livestock species.