• 한국지역사회생활과학회지
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• 제14권2호
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• pp.3-13
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• 2003

This paper studied the present condition of social welfare activities of Living Condition Improving Committee of Kimpo City in order to activate the social welfare services of women agricultural organizations. The main results are as follows; First, the general characteristics of the respondents in this study are forty or fifty years of age with above high school education with main income source of agriculture and middle class women living in agricultural communities more than 10 years. Second, considering the present and future planned activities of the Living Condition Improving Committee of Kimpo City this study finds that there is high expectation of helping poor people and joining voluntary services. Third, the members of the Living Condition Improving Committee of Kimpo City have actively participated in the local welfare service programs however, there is significant difference among people according to the age, living standard and joining period. Fourth, out of social welfare activities managed by the Living Condition Improving Committee of Kimpo City the welfare service activities for old people and local community are most active and the participants in the welfare service activities for women and the youngsters are small. Thus, according to this study the Living Condition Improving Committee of Kimpo City should consider the following three points in order to activate the social welfare service activities; First, as the members are expecting to expand the individual activities and to enhance the private improvement, the social welfare service activities should meet with the demands to improve self satisfaction of the participants. Second, new programs should be developed after reviewing the current programs in order to meet with the need of the members. Third, as the committee has not established a network system with government administrative organizations and relating institutions, and as its activities are concentrated in particular areas the committee should establish a new system to provide more efficient services in line with the cooperation with public organization and welfare resource suppliers.

• 한국미생물·생명공학회지
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• 제38권4호
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• pp.379-384
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• 2010

수집한 전통 장류에서 세포외효소(amylase, protease, cellulase, lipase and fibrinolytic enzyme) 분비능이 우수한 미생물을 분리한 후, 16S rRNA 유전자분석과 생리활성 특성을 조사하였다. amylase와 cellulase 분비능은 모든 선발균주에서 표준균주인 Bacillus subtilis KACC 10114보다 높게 나타났다. Protease 분비능은 D2-14균주를 제외한 모든 선발균주에서 활성이 있었으며, lipase 분비능은 D8-8과 K4-1을 제외한 균주에서 활성이 나타났다. 혈전용해 활성은 D8-2를 제외한 모든 선발균주에서 활성이 나타났으며, D8-8과 K4-1이 가장 높은 활성을 나타냈다. 선발균주인 K3-2, K4-1, K11-1-4, K11-1-6, K11-7, K12-3 와K14-9은 그람 양성세균과 그람 음성세균 그리고 효모에서도 높은 항균활성을 나타내었다. 간장 및 된장에서 분리한 균주들을 동정하기 위해 분자수준에서 16S rRNA 유전자 염기서열을 분석한 결과, 거의 Bacillius sp.로 나타났다. 발효과정에서 세포외효소 분비능과 생리활성이 우수한 미생물을 분리, 확보함으로써 전통 장류의 고급화 및 품질향상과 제조공정의 표준화가 가능할 것으로 여겨진다

• 동아시아식생활학회지
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• 제25권5호
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• pp.880-886
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• 2015

본 연구는 당뇨병, 콜레스테롤 저하작용, 면역조절효과, 관절염 치료 등의 효과가 있다고 알려진 마를 식품소재로써 활용하고자 국내 재배되고 있는 마 품종별 성분을 분석하고, 이용성을 조사하였다. 마 품종별 이화학적 특성 비교 결과, 유리당 함량(mg/g)은 단풍마(28.13) > 장마(24.98) > 산마(20.50) > 둥근마(20.40) 순으로 단풍마가 가장 높았으며, 유기산 함량(mg/g) 또한 단풍마(179.29) > 장마(112.51) > 산마(88.72) > 둥근마(19.23) 순으로 단풍마가 가장 높았다. 조사포닌의 함량(mg/g)은 단풍마(630.3) > 둥근마(66.3) > 장마(42.8) > 산마(38.9) 순으로 단풍마가 가장 높았다. 마의 항산화 활성 결과, 단풍마가 폴리페놀 함량과 DPPH radical 소거능이 높았다. 마의 품종별 성분 특성의 차이를 확인함으로써 마를 활용한 식품 소재화 및 제품 개발을 위한 기초 데이터 자료로 활용될 수 있을 것이라 사료된다.

• 한국환경농학회지
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• 제29권1호
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• pp.39-46
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• 2010

Environmental-friendly agriculture (EFA) is defined as the cultivation of crops with reduced amounts or without chemical-synthetic pesticides. Recently, the use of chemical pesticides has decreased significantly; therefore, we cultivated peppers following EFA- and conventional methods and compared their antioxidant activities. To accomplish this, the environmental-friendly cultivated peppers (EFPE) and conventionally cultivated peppers (CCPE) were extracted with 70% methanol and the effects of the extracts on the cell viability, intracellular ROS generation, lipid peroxidation and catalase activity of HepG2 cells were evaluated. EFPE showed a stronger protective effect against oxidative stress induced-cell death than that of CCPE. EFPE also reduced intracellular ROS generation (42.7% to 26.4%) following treatment with hydrogen peroxide more effectively than that of CCPE (24.2% to 6.3%). Furthermore, EFPE and CCPE showed protective effects against lipid peroxidation and induced catalase activity, although these effects were not statistically significant. Taken together, these results suggest that EFPE showed stronger antioxidant activities than CCPE, and thus represent evidence that EFA with biocontrol materials may improve the functional properties of crops and/or secondary metabolites with antioxidant activities when compared with conventional agricultural practices.

• 한국잠사곤충학회지
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• 제48권2호
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• pp.68-72
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• 2006

본 연구는 식물병원성곰팡이에 대한 곤충 혈액 및 유충체의 항곰팡이 활성을 조사하였다. 집누에, 장수풍뎅이, 흰점박이 꽃무지 모두 곰팡이 저해활성을 나타냈으며, 집누에의 계통간 비교에서는 1087계통의 활성이 가장 높았다. 또한, 혈액보다는 유충체를 이용한 경우가 더 강한 항곰팡이 활성을 나타냈다. 곤충의 항곰팡이 활성은 곰팡이균주에 따라 다르며, 본 연구에 사용된 3종은 A. panax, C. gloeosporioides 및 P. oryzae 균에 항곰팡이 활성을 가진다.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.69.1-69.1
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• 2008

• Journal of Microbiology and Biotechnology
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• 제22권2호
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• pp.264-269
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• 2012

Recently, we reported that the synthetic Coprisin analog peptide 9-mer dimer CopA3 (consisted of all-L amino acid sequence) was designed based on a defensin-like peptide, Coprisin isolated from Copris tripartitus. The 9-mer dimer CopA3 (L-CopA3) had antibacterial activity and induced apoptosis in human leukemia cells via a caspase-independent pathway. In this study, all of amino acid sequences of L-CopA3 were modified to all D-form amino acids (DCopA3) to develop a more effective antimicrobial peptide. We investigated whether D-CopA3 had antimicrobial activities against pathogenic microorganisms and pro-apoptotic effects in human leukemia cells (U937, Jurkat, and AML-2). The synthetic peptide D-CopA3 had antimicrobial activities against various pathogenic bacteria and yeast fungus with MIC values in the 4~64 ${\mu}M$ range. Moreover, D-CopA3 caused cell growth inhibition, and increased the chromosomal DNA fragmentation and the expression of inflammatory cytokines, TNF-${\alpha}$ and IL1-${\beta}$, transcripts in human leukemia cells. The all-D amino acid peptide DCopA3 proved as effective as the L-CopA3 reported previously. These results provide the basis for developing D-CopA3 as a new antibiotic peptide.

• 농촌지도와개발
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• 제4권1호
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• pp.269-281
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• 1997

This study intended to compare rural development programs between China and Thailand, focused on research and extension activities. In China, Agricultural Science Administration(ASC) took charges in agricultural research, and under the ASC there were 39 agricultural organizations throughout the country. There were distinctive differences as to condition of agricultural areas, under-developed area at middle and developing area by sea and river. To improve agricultural production in China, diffusion of new agricultural technology would be important along with supplying equipment such as soil test kits, plant nutrition analysis sets and vehcles for extension educators. In Thailand, under the Ministry of Agriculture & Cooperatives, there were five departments, seven divisions and eight national enterprises. Among them, Department of Agriculture was in charge of agricultural research while the Department of Agricultural Extension for extension services. Each department had sub-division and provincial organization including personnel management and budget. However, the relationships and consistency between the Department of Agriculture and the Department of Agricultural Extension needed to be improved for better cooperation of research and extension.

• 한국식품저장유통학회지
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• 제22권4호
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• pp.520-527
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• 2015

마늘의 냉동에 따른 생리활성을 변화를 분석함으로서생리활성 효능이 유지되는 최적의 냉동 온도를 선정하고자 냉동조건에 따른 마늘 추출물의 항산화 및 항균활성을 비교분석하였다. 마늘 추출물의 페놀 화합물 함량은 $6.91{\pm}0.69{\mu}gGAE/g$으로 냉동 조건(-20, -80, $-196^{\circ}C$)에 따라 큰 차이를 보이지 않았으나, 플라보노이드 함량은 $-80^{\circ}C$에서 $0.327{\pm}0.058{\mu}gQE/g$에 반해 $-20^{\circ}C$ 에서 $0.263{\pm}0.015{\mu}gQE/g$으로 소폭 감소를 나타내었다. DPPH 라디칼 소거능 분석에서는 2 mg/mL의 추출물에서 $4^{\circ}C$와 $-80^{\circ}C$에서 61%로 나타난 것에 비해 $-20^{\circ}C$와 $-196^{\circ}C$에서는 51%가량으로 10%정도 라디칼 소거능이 감소하는 경향을 나타내었고, 마늘 추출물의 SOD 유사활성은 $4^{\circ}C$ 대조군에 비해 냉동조건에서 전반적으로 증가 경향을 나타냈다. 아질산염 소거능은 pH 3.0 조건에서 18% 수준에서 소폭 증가가 나타난 반면 환원력 비교에서는 700 nm의 흡광도가 1.6 abs 수준에서 냉동조건에 따라 감소하는 경향을 나타내었고 $-80^{\circ}C$에서 감소폭이 가장 적게 나타났으나 유의적인 수준은 못 되었다. 항균활성 비교 실험에서는 마늘 추출물이 E. coli, S. typhimurium, V. vulnificus, S. aureus와B. cereus에 대해 높은 항균활성을 나타냈으며, 마늘의 냉동 조건에 따라서는 유의적 차이를 보이지 않았다. 이상의 결과를 종합하여 볼 때 마늘의 냉동후 추출물의 생리활성은 실험조건에 따른 변화 폭이 크지는 않지만 페놀 화합물과 플라보노이드 및 이에 의한 항산화 및 항균 활성이 안정적으로 유지되는 최적 온도는 $-80^{\circ}C$로서, 마늘의 냉동 저장 중 품질특성 뿐만 아니라 생리활성기능 유지라는 두 가지 측면에서 $-20^{\circ}C$ 관행 냉동보다는 저온 급속냉동이 필요하다고 판단된다.

• 한국식품영양학회지
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• 제32권6호
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• pp.630-635
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• 2019

The purpose of this study was to determine the content of polyphenols and flavonoids, vitamin C, and antioxidant activity for the extract from the Deodeok sprout. To accomplish this, the Deodeok sprout whole (CLS-W), above ground part (leaf, stem, CLS-L), and root (CLS-R) were individually extracted using 70% ethanol. The highest levels of total polyphenols and total flavonoids were observed in the Deodeok sprout extract CLS-L2. Similarly, antioxidant activities resulting in radical scavenging activities increased significantly in the extract of CLS-L2. In conclusion, these results indicate that Deodeok sprouts can be used as a viable, new natural antioxidant source.