• The Korean Journal of Mycology
• /
• v.18 no.2
• /
• pp.77-83
• /
• 1990

Factors influencing the fusion frequency of protoplasts were investigated with auxotrophic mutants of Pleurotus florida and Pleurotus ostreatus. Immediately after the polyethylene glycol (PEG) solution was added, the protoplasts adhered firmly and shrank. During the subsequent dilution with 0.6 M sucrose, the protoplasts regained their normal size and larger bodies were observed. Interspecific heterokaryons were obtained by fusion of the nutritionally complementing protoplasts. Hyphae of the heterokaryotic fusants formed true clamp connections. The optimum conditions were a total of 1 to 15 million protoplasts per ml, 30% polyethylene glycol 8000 solution with adjustment to pH 8.0 and 0.6 M sucrose stabilized regeneration medium. Other parameters such as $CA^{++}$, glycine, exposure time and temperature influenced mainly the viability of the protoplasts.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.18 no.1
• /
• pp.13-17
• /
• 2009

The caterpillar-shaped Chinese medicinal mushroom (DongChongXiaCao) looks like a worm in the winter and like a grass in the summer. The fruiting body has been regarded as popular folk or effective medicines used to treat human diseases such as asthma, bronchial and lung inflammation, and kidney disease. The fruiting bodies of Cordyceps militaris, C. pruinosa and Paecilomyces tenuipes that formed on the living silkworm (Bombyx mori) host were used in this examination. This study was carried out to investigate the soluble sugar, amino acid and fatty acid profiles in the fruiting-bodies. Soluble sugars such as glycerol, glucose, mannitol and sucrose were mainly found in the fruiting bodies of C. militaris, C. pruinosa and P. tenuipes. Total soluble sugar content was 29.23 mg/g in C. militaris, 8.61 mg/g in C. pruinosa and 24.00 mg/g 1in P. tenuipes on dry weight basis. Total free amino acid content was 14.09 mg/g 1in C. militaris, 34.60 mg/g in C. pruinosa and 17.09 mg/g in P. tenuipes. The content of oleic acid in fatty acids was above high more than 30% regardless of species.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2020.06a
• /
• pp.117-117
• /
• 2020

• Journal of Drive and Control
• /
• v.19 no.4
• /
• pp.46-53
• /
• 2022

RIn this study, a test tractor that could measure various types of agricultural operational loads was developed, and its performance was verified. This tractor could be used to measure the load generated during agricultural work and convert the related data into a database. A test tractor was developed using an 80-kW-rated load tractor, and it could measure various types of field test data, such as engine torque and rpm, wheel torque, PTO(power take-off) torque, hexometer, IMU/INS sensor, steering angle sensor, hydraulic pressure, and flow sensor data. To verify the developed test tractor, a verification test using an agriculture rotavator was performed. The test conditions were L1, L2, and L3 based on the tractor's main and sub-transmission stages, and stages 1 and 2 were selected as the PTO. In a comparison of the analyzed test data, similar tendencies in the test results of this research and other research (Kim's research) were seen. Through this, the developed test tractor was verified. In the future, we plan to conduct research on the tractor developed in this study using various attached working machines.

• Asian-Australasian Journal of Animal Sciences
• /
• v.2 no.3
• /
• pp.319-321
• /
• 1989

• Journal of Sericultural and Entomological Science
• /
• v.48 no.1
• /
• pp.16-20
• /
• 2006

To shorten the labor cost for the production of the fungus, Paecilomyces tenuipies, uncocooning mutant hybrid silkworms as a host was used. The yield of P. tenuipies was reduced when Nd (uncocooning mutant) was used as a host because Nd was smaller than the hybrid Baegokjam that has been used as a typical host. To increase the host weight, Nd was crossed with Jam l23 or Jam 124, and resulted in not only greater weighter but also higher yield of P. tenuipies. The optimum temperature for rearing Nd hybrid silkworms was $3^{\circ}C$ higher than standard temperature of rearing silkworms to increase the naked pupation rate. The optimum time for the infection of P. tenuipies was 12 h at $28.5^{\circ}C$ and 95% R.H., when $1{\times}10^7spores/ml$ of P. tenuipies were sprayed on the surface of the silkworm. The conditions were applicable to both spring-rearing and autumn-rearing.

• Journal of Korean Society of Rural Planning
• /
• v.9 no.3 s.20
• /
• pp.41-46
• /
• 2003

The study was focused on setting priority for future core technologies in rural development and resources using AHP. The technologies were derived by Delphi method. Evaluation criteria for the priority setting were decided as 'technology', 'market oriented', and 'public concerns' by council. The future core technologies were divided as four groups by importance and R&D level. Technologies in upper two groups were considered in the study. Group I had high importance and high R&D level. Group II had high importance and lower R&D level. Questionnaires were given to 9 specialists in agricultural resources. As the results, 'public concerns' was decided as a most important evaluation criterion. The most important technologies are "technology developing for animal feces and urine treatment, and restoration it to farm land" in group I, and "technology developing for agricultural by-products as energy source" in group II.

• Journal of Korea Technology Innovation Society
• /
• v.2 no.3
• /
• pp.107-117
• /
• 1999

This study was conducted to provide a improving direction of R&D investment for agricultural technology. The results is summerized as follows : 1) it is necessary to make a systematic master-plant of R&D investment for agricultural technology especially including management system 2) Reinforcement of researchers and investment related with agricultural technology might be im-portant factor 3) To clarify the roles by organization is necessary to efficient management for this is needed to efficient use of research resource 5) In the meeting pro-vice era a synthetic management of tech-nology developed by researchers and mutual exchange between research organization researcher is necessary. Finally Builing up a information delivery system of agricultural technology compatible to use the technology developed is recommended.

• The Korean Ginseng Research and Industry
• /
• v.13 no.1
• /
• pp.3-10
• /
• 2019

• Mycobiology
• /
• v.36 no.1
• /
• pp.50-54
• /
• 2008

This study examined the chemical composition of A. blasiliensis and the chemical structural properties of an immuno-stimulating polysaccharide. The amino acids, free sugars, and organic acids by HPLC and fatty acids by GC were analyzed. The immuno-stimulating substance from A. blasiliensis was extracted with hot water and purified by ethanol precipitation. It underwent ion exchange chromatography on DEAE-cellulose and gel filtration on Toyopearl HW 65F. Through GP-HPLC, the substance was found to be homogeneous. Its chemical structure was determined by $^{13}C-NMR$. Fatty acids, organic acids, and sugar alcohol composition consisted exclusively of linoleic acid, fumaric acid and mannitol, respectively. The amino acids were mainly glutamic acid, glycine, and arginine. By $^{13}C-NMR$ analysis, the immuno-stimulating substance was identified as ${\beta}-(1{\rightarrow}3)\;(1{\rightarrow}6)$-glucan, composed of a backbone with $(1{\rightarrow}3)$-linked D-glucopyranosyl residues branching a $(1{\rightarrow}6)$-linked D-glucopyranosyl residue. The ${\beta}$-glucan from A. blasiliensis showed pronounced immuno-stimulating activity on the antibody-production ability of B-lymphocytes by the hemolytic suspension assay. In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.