• Asian-Australasian Journal of Animal Sciences
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• 제30권12호
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• pp.1739-1750
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• 2017

Objective: This experiment investigated meat color, myoglobin content, enzyme activities, and expression of genes associated with oxidative potential of pigs slaughtered at different growth stages. Methods: Sixty 4-week-old $Duroc{\times}Landrace{\times}Yorkshire$ pigs were assigned to 6 replicate groups, each containing 10 pigs. One pig from each group was sacrificed at day 35, 63, 98, and 161 to isolate longissimus dorsi and triceps muscles. Results: Meat color scores were higher in pigs at 35 d than those at 63 d and 98 d (p<0.05), and those at 98 d were lower than those at 161 d (p<0.05). The total myoglobin was higher on 161 d compared with those at 63 d and 98 d (p<0.05). Increase in the proportions of metmyoglobin and deoxymyoglobin and a decrease in oxymyoglobin were observed between days 35 and 161 (p<0.05). Meat color scores were correlated to the proportion of oxymyoglobin (r = 0.59, p<0.01), and negatively correlated with deoxymyoglobin and metmyoglobin content (r = -0.48 and -0.62, p<0.05). Malate dehydrogenase (MDH) activity at 35 d and 98 d was higher than that at 161 d (p<0.05). The highest lactate dehydrogenase/MDH ratio was achieved at 161 d (p<0.05). Calcineurin mRNA expression decreased at 35 d compared to that at 63 d and 98 d (p<0.05). Myocyte enhancer factor 2 mRNA results indicated a higher expression at 161 d than that at 63 d and 98 d (p<0.05). Conclusion: Porcine meat color, myoglobin content, enzyme activities, and genes associated with oxidative potential varied at different stages.

• 한국미생물·생명공학회지
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• 제38권4호
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• pp.379-384
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• 2010

수집한 전통 장류에서 세포외효소(amylase, protease, cellulase, lipase and fibrinolytic enzyme) 분비능이 우수한 미생물을 분리한 후, 16S rRNA 유전자분석과 생리활성 특성을 조사하였다. amylase와 cellulase 분비능은 모든 선발균주에서 표준균주인 Bacillus subtilis KACC 10114보다 높게 나타났다. Protease 분비능은 D2-14균주를 제외한 모든 선발균주에서 활성이 있었으며, lipase 분비능은 D8-8과 K4-1을 제외한 균주에서 활성이 나타났다. 혈전용해 활성은 D8-2를 제외한 모든 선발균주에서 활성이 나타났으며, D8-8과 K4-1이 가장 높은 활성을 나타냈다. 선발균주인 K3-2, K4-1, K11-1-4, K11-1-6, K11-7, K12-3 와K14-9은 그람 양성세균과 그람 음성세균 그리고 효모에서도 높은 항균활성을 나타내었다. 간장 및 된장에서 분리한 균주들을 동정하기 위해 분자수준에서 16S rRNA 유전자 염기서열을 분석한 결과, 거의 Bacillius sp.로 나타났다. 발효과정에서 세포외효소 분비능과 생리활성이 우수한 미생물을 분리, 확보함으로써 전통 장류의 고급화 및 품질향상과 제조공정의 표준화가 가능할 것으로 여겨진다

• Journal of Microbiology and Biotechnology
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• 제16권4호
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• pp.511-518
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• 2006

A gene cluster for cyclohexanone oxidation was cloned from Rhodococcus sp. TK6, which is capable of growth on cyclohexanone as the sole carbon source. The 9,185-bp DNA sequence analysis revealed seven potential open reading frames (ORFs), designated as ssd-chnR-chnD-chnC-chnB-chnE-partial pcd. The chnBCDE genes encode enzymes for the four-step conversion of cyclohexanone to adipic acid, catalyzed by cyclohexanone monooxygenase (ChnB), $\varepsilon-caprolactone$ hydrolase (ChnC), 6-hydroxyhexanoate dehydrogenase (ChnD), and 6-oxohexanoate dehydrogenase (ChnE). Furthermore, the presence of a regulatory element in the downstream region of the chnD gene supports the notion that chnR is a putative regulatory gene. Among them, the activity of ChnB was confirmed and characterized, following their expression and purification in Escherichia coli harboring the modified chnB gene (chnB gene with 6 successive codons for His at the 3' terminus).

• 한국축산시설환경학회지
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• 제14권1호
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• pp.47-52
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• 2008

1. 조사농장의 산란율은 하이라인, 이사브라운 모두 $85{\sim}93%$ 수준 이였으며, 농가자체 파란율은 하이라인 $1.5{\sim}2.2%$, 이사브라운 $1{\sim}1.4%$이었다. 2. 계란 이송단계별 파란발생비율은 집란벨트 4.7%> 엘리베이터 2.5%> 선란기 1.7%> 컨베이어 0.9% 순 이었다. 3. 집란벨트에서 엘리베이터로 이송 중 계란이송가이드를 설치할 경우 약 1%의 파란경감 효과가 있는 것으로 나타났다. 4. 하이라인 브라운은 주령이 높아지면서 파란율은 높아지고, 파각력은 낮아지는 것으로 나타났으며, 회귀계수 분석 유의성은 $R^2=0.7$로 파각력의 변화가 파란율에 영향을 미치는 것으로 분석되었다. 5. 이사브라운에서는 환우 전에 비하여 환우 후 파각력이 높아진 것으로 나타났다. 6. 난각 두께와 파각력, 그리고 접촉 후 파각변위와 파각력과의 관계에서 이들 함수의 변수간에 회귀관계는 유의성이 나타나지 않았다.

• 식물병연구
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• 제15권1호
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• pp.1-7
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• 2009

Viruses diagnosed on crops including rice plants from farmers or agricultural extension agencies cover the country were 11 species including Broad bean wilt virus 2 (BBWV2) in 2008. Tomato spotted wilt virus (TSWV) was the most important virus having the detection rate of 22.9%. Two viruses of Tomato yellow leaf curl virus (TYLCV) and Tobacco leaf curl virus (TLCV) inducing leaf yellow and curl diseases on tomatoes were occurred newly with the detection rate of 12.2% and 4.0%, respectively, in 2008. Rice stripe virus (RSV) was occurred on 869.5 ha mainly at Jindo and Haenam areas in Jeollanamdo province. At Jindo area, 12 plots were damaged severely with the infected hill rate of 83.8%. At the main production area of oriental melon at Seongju, almost all fruits from whole sale market at Seongju were infected with Cucumber green mottle mosaic virus (CGMMV) as the detection rate of 87%. The areas occurred TSWV in Korea were 25 totally from 2003 including 7 areas newly reported in 2008 including Naju in Jeoallanamdo. TSWV could be reduced as 0.1 % from 5.3% by covering insect proof net in vinyl house after chemical soil sterilization. Tomato yellow leaf curl disease was occurred on April in 2008 at Tongyoung area in Kyeongsangnamdo, and detected continuously at 13 areas, 7 in Kyeongsangnamdo, 4 in Jeollabukdo and 2 in Jejudo. Potato spindle tuber viroid (PSTVd) was occurred abruptly in a confined space of a civil breeding greenhouse and a cultivar evaluation field followed by disuse 17.4 M/T of potato tubers. No PSTVd was detected at 17 fields cultivated the related potatoes to the bred company by RT-PCR.

• Mycobiology
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• 제51권6호
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• pp.388-392
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• 2023

A yeast strain, designated as JAF-11^T, was isolated from flower of Prunus mume Sieb. et Zucc. in Gwangyang, Republic of Korea. Phylogenetic analysis showed that strain JAF-11^T was closely related to Neodothiora populina CPC 39399^T with 2.07 % sequence divergence (12 nucleotide substitutions and three gaps in 581 nucleotides) in the D1/D2 domain of the large subunit (LSU) rRNA gene, and Rhizosphaera macrospora CBS 208.79^T with 4.66 % sequence divergence (25 nucleotide substitutions and five gaps in 535 nucleotides) in the internal transcribed spacer (ITS) region. Further analysis based on the concatenated sequences of the D1/D2 domain of the LSU rRNA gene and the ITS region confirmed that strain JAF-11^T was well-separated from Neodothiora populina CPC 39399^T. In addition to the phylogenetic differences, strain JAF-11^T was distinguished from its closest species, Neodothiora populina CPC 39399^T and Rhizosphaera macrospora CBS 208.79^T belonging to the family Dothioraceae by its phenotypic characteristics, such as assimilation of carbon sources. Hence, the name Neodothiora pruni sp. nov. is proposed with type strain JAF-11^T (KACC 48808^T; MB 850034).

• 생물환경조절학회지
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• 제14권4호
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• pp.269-274
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• 2005

실파용으로 적정 양액농도를 구명하고자 수경재배용으로 선발한 '금장외대파'와 '토쿄구로파' 품종을 공시하여 담액식으로 하여 시험을 수행하였다. 양액종류 시험을 통해 선발된 야마자키 처방의 싹파 전용 양액$(NO_3^--N\;9.0,\;NH_4^+-N\;3.0,\;PO_4^{3-}-P\;6.0,\;K^+7.0,\;Ca^{2+}\;2.0,\;Mg^{2+}\;2.0,\;and\;SO_4^{2-}-S\;4.4me{\cdot}L^{-1})$을 이용하여 EC 0.6, 1.2, 1.8, $2.4dS{\cdot}m^{-1}$의 4수준으로 처리한 결과 초장, 구당 생체중과 건물중에 있어서 두 품종 모두 EC 1.2, 1.8, 2.4, $0.6dS{\cdot}m^{-1}$ 순으로 양호하였다. 최대 생체중을 위한 남장외대파와 '토쿄구로파'의 2차 회귀곡선식은 $y=-42.091x^2+171.79x+11.047 (R^2=0.8946,\; R=0.9458^*)$와 y=-50.069x2+157.58x+15.414$(R^2=0.9343,\;R=0.9692^{**})$ 이었고, 이에 따른 적정 양액농도는 각각 $1.68dS{\cdot}m^{-1}$과 $1.57dS{\cdot}m^{-1}$수준이었다. 따라서 실파 수경재배시 생육 초기에는 양액을 $1.2dS{\cdot}m^{-1}$수준의 저농도로 유지하고 정식 후 30일경인 생육 중기 이후에는 $1.6\~1.7dS{\cdot}m^{-1}$수준의 농도로 유지하는 것이 효율적이라 여겨진다.

• 한국기계가공학회지
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• 제20권6호
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• pp.92-99
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• 2021

The rotavator is attached to the three-point hitch at the rear of a tractor and uses the power take-off strength of the tractor to perform soil harrowing. During operation, the power transmitted to the gearbox of the rotavator varies with the soil characteristics and depth. These properties influence the reliability of the gearbox. In this study, actual load measurements and analyses were performed using a rotavator. In addition, the safety factor and fatigue life of the gearbox components were determined using the analysis results. Through analysis and tests, the contact pattern of the gear tooth surface was identified. The input power values of the gearbox were minimum and maximum at 54.5% and 84.5% of the tractor power, respectively. Based on the actual load analysis results, the strength and fatigue life of the gearbox components were satisfied. In addition, through the analysis and testing of the gear contact pattern, it was confirmed that a similar contact occurred. Through the analysis, the magnitude of the load acting on the tooth surface of the gear was confirmed.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.256-257
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• 2010

• Asian-Australasian Journal of Animal Sciences
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• 제27권2호
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• pp.179-186
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• 2014

This study aimed to investigate the diversity of the Butyrivibrio group bacteria in goat rumen and its response to garlic oil (GO) supplementation as revealed by molecular analysis of cloned 16S rRNA genes. Six wethers fitted with ruminal fistulas were assigned to two groups for a cross-over design with 28-d experimental period and 14-d interval. Goats were fed a basal diet without (control) or with GO ruminal infusion (0.8 g/d). Ruminal contents were used for DNA extraction collected before morning feeding on d 28. A total bacterial clone library was firstly constructed by nearly full-length 16S rRNA gene cloned sequences using universal primers. The resulting plasmids selected by Butyrivibrio-specific primers were used to construct a Butyrivibrio group-specific bacterial clone library. Butyrivibrio group represented 12.98% and 10.95% of total bacteria in control and GO group, respectively. In libraries, clones were classified to the genus Pseudobutyrivibrio, Butyrivibrio and others within the family Lachnospiraceae. Additionally, some specific clones were observed in GO group, being classified to the genus Ruminococcus and others within the family Ruminococcaceae. Based on the criterion that the similarity was 97% or greater with database sequences, there were 29.73% and 18.42% of clones identified as known isolates (i.e. B. proteoclasticus and Ps. ruminis) in control and GO groups, respectively. Further clones identified as B. fibrisolvens (5.41%) and R. flavefaciens (7.89%) were specifically found in control and GO groups, respectively. The majority of clones resembled Ps. ruminis (98% to 99% similarity), except for Lachnospiraceae bacteria (87% to 92% similarity) in the two libraries. The two clone libraries also appeared different in Shannon diversity index (control 2.47 and GO group 2.91). Our results indicated that the Butyrivibrio group bacteria had a complex community with considerable unknown species in the goat rumen.