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Cloning and Characterization of a Gene Cluster for Cyclohexanone Oxidation in Rhodococcus sp. TK6  

Choi Jun-Ho (Department of Agricultural Chemistry, Kyungpook National University)
Kim Tae-Kang (Department of Agricultural Chemistry, Kyungpook National University)
Kim Young-Mog (Institute of Agricultural Science & Technology, Kyungpook National University)
Kim Won-Chan (Department of Agricultural Chemistry, Kyungpook National University)
Park Kunbawui (Department of Agricultural Chemistry, Kyungpook National University)
Rhee In-Koo (Department of Agricultural Chemistry, Kyungpook National University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.4, 2006 , pp. 511-518 More about this Journal
Abstract
A gene cluster for cyclohexanone oxidation was cloned from Rhodococcus sp. TK6, which is capable of growth on cyclohexanone as the sole carbon source. The 9,185-bp DNA sequence analysis revealed seven potential open reading frames (ORFs), designated as ssd-chnR-chnD-chnC-chnB-chnE-partial pcd. The chnBCDE genes encode enzymes for the four-step conversion of cyclohexanone to adipic acid, catalyzed by cyclohexanone monooxygenase (ChnB), $\varepsilon-caprolactone$ hydrolase (ChnC), 6-hydroxyhexanoate dehydrogenase (ChnD), and 6-oxohexanoate dehydrogenase (ChnE). Furthermore, the presence of a regulatory element in the downstream region of the chnD gene supports the notion that chnR is a putative regulatory gene. Among them, the activity of ChnB was confirmed and characterized, following their expression and purification in Escherichia coli harboring the modified chnB gene (chnB gene with 6 successive codons for His at the 3' terminus).
Keywords
Cyclohexanope oxidation; cyclohexanone monooxygenase; Rhodococcus sp. TK6;
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Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
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