• 한국균학회지
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• 제25권4호통권83호
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• pp.305-310
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• 1997

P. eryngii균의 톱밥 인공재배에 관한 연구를 수행한 결과 균사배양 조건에 관해서 얻은 결과를 요약하면 다음과 같다. P. eryngii균의 균사배양 최적배지는 Lilly배지에서 균사생장량이 가장 많았고, 균사배양 최적온도는 $25{\sim}30^{\circ}C$,최적 pH는 6.0이었다. 최적 탄소원은 단당류인 경우 glucose, 다당류에서는 dextrin 이었으며, 최적 농도는 glucose $3{\sim}4%$, dextrin은 5%수준에서 균체량이 가장 많았다. 또 최적 질소원은 유기태 질소인 casamino acid에서 균체량이 가장 많았으며 최적농도는 0.12%였다.

• Journal of Biosystems Engineering
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• 제25권6호
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• pp.481-488
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• 2000

This study was initiated in order to find alternative fuel substituting for light oil the most common fuel for heating greenhouse. The tire oil used in this research was produced by pyrolysis process, one of the final products besides steel string and carbon black in which waste tires as a form of chopped pieces broken by shredding machine are heated up to 200~30$0^{\circ}C$ with maximum restraining of oxygen supply. In order to justify light oil equivalent qualities in tire oil combustion characteristics were defined in the way of comparing kinetic viscosities in the wide range of temperature flame sizes and exhaust gas components in the various combustion conditions. We found that kinetic viscosity of tire oil was lower than light oil by 1 to 2 cSt in the temperature range showing better flowing mobility in the fuel line of the burner and no significant difference in flame size between the two oils in the all combustion treatments. However much more NO and SO$_2$ were detected from the exhaust gases of tire oil than light oil combustions. In fact tire oil contains more nitrogen and total sulfur, by 25 times and 40 times respectively than light oil according to the composition analysis. Tolerable limit for SO$_2$discharge amount defined by the national air pollution standards is under 540ppm so tire oil combustion satisfies the requirement though. It is desirable if sulfur and nitrogen filtering process shall be added in the tire oil production line. Except the exhaust gas components all greenhouse heating qualities of tire oil including hot air temperature are very identical to those of light oil.

• 한국농림기상학회:학술대회논문집
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• 한국농림기상학회 2018년도 하계 학술발표초록집
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• pp.115-116
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• 2018

• Mycobiology
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• 제45권2호
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• pp.110-113
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• 2017

Severe root rot was observed in fields of cabbages (Brassica oleracea L.) in 2015 in China. Cardinal symptoms of this disease included root rot and wilting leaves. A fungus was isolated from diseased tissues consistently. Based on the morphological features and molecular analysis of the ITS-5.8S rDNA and D1/D2 domain of the 28S rRNA gene, it was identified as Plectosphaerella cucumerina. This is the first report of P. cucumerina causing cabbage root rot in China and the world.

• Journal of Animal Science and Technology
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• 제46권1호
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• pp.1-6
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• 2004

본 연구는 MCIR$^*$3 allele의 돼지에 있어서 유전적 변이를 관찰하기 위하여 수행하였다. 일반적으로 흑모색 바탕에 백색반점이나 백색띠를 갖고 있는 돼지의 MCIR 유전자의 유전자형은 E$^{D2}$로 나타낸다. 우성 백색계통의 E$^P$ 유전자형은 우성 흑모색 계통의 E$^{D2}$ 유전자와 frameshift mutation 관계가 있다. 돼지 MCIR 전체 번역지역을 증폭하기 위하여 oligonucleotide primer률 제작하여 PCR을 수행 하였다. 그 결과 길이가 963${\sim}$966 base pairs인 돼지 MCIR 유전자의 전체번역지역을 포함하는 산물을 얻었다. 이들 번역부위의 염기서열 결정하고 이들을 Clusta1 W 프로그램을 이용하여 정렬한 결과 23번 코돈{nt68)에서 Hampshire와 제주 재래혹돈은 염기 시토신(cytosine)이 3 개 그리고 Birl‘shire의 경우 염기 시토신(cytosine)이 2개 결실되어 있었다. 그 외에 3개의 missense mutations과 하나의 frameshift mutation이 발견되었다.

• 농촌지도와개발
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• 제23권4호
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• pp.419-429
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• 2016

This study focuses on the policy priorities for training agro-healing experts. Training agro-healing experts is an important task that should boost local community in rural areas. In order to make an efficient decision making, the study analyses priority of relevant policies using the AHP(Analytic Hierarchy Process) method. According to the results, R&D comes out first. Human Resource Development(HRD), financial support, and the construction of legal system comes after R&D. In R&D, qualifications and guidelines for participants comes out the most significant issue. In the aspect of HRD, it is very important to develop and utilize regional personnel such as unemployed youth and retirees from related fields. As for financial support, funding for educational facilities (i.e. classrooms and farmland for practice) is needed. In case of legal system, it is desirable to introduce the recruiting experts system and qualification system certified by government.

• 한국환경농학회지
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• 제42권1호
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• pp.63-70
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• 2023

Golden apple snails (Pomacea canaliculat) (GAS) are widely used for weed control in rice cultivation. However, concerns on the ecological risk of invasive GAS species are increasing. This study aimed to evaluate the overall impacts of GAS on weed control, abundance of aquatic animals and insects, and rice yield, in comparison with that from other biocontrol agents such as loach (Misgurnus mizolepis) and catfish (Silurus asotus), which are alternatives for biological weed control in rice paddy cultivation. Field experiments included five treatments; control, herbicide, GAS, loach, and catfish. During the rice growth, weed appearance and biological abundance were monitored, and at harvest, the rice yield was determined. Weed control efficiency was the highest for GAS treatment (100%), followed by that for herbicide (95.8%), loach (57.5%), and catfish treatments (31.7%). Insect abundance was considerably decreased in GAS treatment due to heavy weed removal, which affects the habitat of aquatic animals and insects. The amount of rice yield (unit: kg 10 a^-1) was in the order of GAS (798.9) > loach (708.1) = herbicide (700.7) > catfish (629.4) > control (496.0). Therefore, considering the weed control efficiency, biological abudance, and rice yield, loach could be a potential alternative for biological weed control in organic rice farming. However, the activity of the biocontrol agents are susceptible to environmental conditions; therefore, further studies under different conditions are essential to confirm these findings.

• Applied Biological Chemistry
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• 제46권3호
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• pp.246-250
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• 2003

양하(Zingiber mioga Roscoe) 근경의 에탄을 추출물을 재료로 Staphylococcus aureus, Bacillus subtilis 및 B. cereus에 대한 activity-guided fractionation을 실시하여 항균성 물질 세가지준 얻었다. 항균력실험 결과, 화합물 I과 111은 세가지균주 모두에 활성이 있었고, 화합물 II에서는 B. subtilis과 B. cereus에서만 활성이 나타났다. 그 중에서 가장 활성이 강한 화합물 I을 Bioscreen C로 optical density(600 nm)를 측정하여 증식억제실험을 한 결과 10 ppm 처리시 B. subtilis과 B. cereus에서 72시간동안 강한 증식억제효과를 나타내었으며, S. aureus에서는 25 ppm 처리시 72시간동안 완전증식억제효과를 나타내었다. $1^H-NMR$, ${13}^C-NMR$, DEPT,$1^H-1^H$ COSY, HMQC, HMBC 및 IR 스펙트럼 등을 분석한 결과 화합물 I, II, III은 labdane-type diterpene인 $(E)-8{\beta}(17)-epoxylabd-12-ene-15,16-dial(C_{20}H_{30}O_3,\;MW=318)$, $galanolactone(C_{20}H_{30}O_3,\;MW=318)$ 그리고 galanal A($C_{20}H_{30}O_3,\;MW=318$)로 각각 동정되었으며, 이들은 양하의 근경에서는 처음 분리된 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권3호
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• pp.340-350
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• 2007

The objective of this study was to determine whether peroxisome proliferator-activated receptor ${\gamma}$(PPAR${\gamma}$ is involved in the regulation of weaning to estrus of primiparous sows. Twelve sows composed of 6 groups of 2 full-sibs in a similar age (325.2 d), body weight (BW; 152.4 kg) and backfat thickness (BFT; 27.0 mm) at start of lactation, were allocated to accept 31 MJ (restricted group, R-group) or 53 MJ (control group, C-group) DE/d treatment, respectively. The experimental results indicated that the low energy intake resulted in excessive losses of BW and BFT during lactation in R-group sows, which may be related to decrease of serum 15-deoxy-${\Delta}^{12,14}$-prostaglandin $J_2$ (15d-$PGJ_2$), a ligand of PPAR${\gamma}$ The obvious peak and the frequency of LH, FSH and estradiol ($E_2$) were only observed in C-group sows. Except for $E_2$ at d 1 and 2, serum FSH, LH and $E_2$ concentrations in R-group were lower than those in C-group sows after weaning. However, the serum progesterone ($P_4$) level in R-group sows was always more than that in C-group. The expression abundances of PPAR${\gamma}$and GnRH receptor (GnRH-R) in pituitary, FSH receptor (FSH-R), LH receptor (LH-R), estrogen receptor (ES-R) and aromatase in ovary of anestrous sows were lower than those of estrous sows. Neither the BFT nor the BW was associated with the mRNA abundance of PPAR${\gamma}$in hypothalamus during lactation. Expressions of PPAR${\gamma}$in pituitary and ovary were affected evidently by the BFT changes and only by the loss of BW of sows during and after lactation. Furthermore, PPAR${\gamma}$mRNA level in ovary was significantly related to the expression abundances of GnRH-R, FSH-R, ES-R and aromatase, and GnRH-R was obviously associated with PPAR${\gamma}$expression in pituitary. However, PPAR${\gamma}$expression in hypothalamus likely has no effects on these genes expression and no obvious difference for all sows. Not serum $E_2$ or $P_4$ alone but the ratios of $E_2$ to $P_4$ and 15d-$PGJ_2$ to $P_4$, and serum FSH and LH were evidently related to PPAR${\gamma}$expression in pituitary and ovary. It is concluded that PPAR${\gamma}$is associated with body conditions, reproduction hormones and their receptor expression, which affected the functions of pituitary and ovary and ultimately the estrus after weaning of primiparous sows.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 춘계학술발표회 요지
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• pp.272-273
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• 2005