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http://dx.doi.org/10.5187/JAST.2004.46.1.001

Detection of Novel Genetic Variations of the MG1R * 3 Allele in Pig(Sus scrofa)  

Cho, I.C. (National Jeju Agricultural Experiment Station, R.D.A)
Jeong, Y.H. (National Jeju Agricultural Experiment Station, R.D.A)
Jung, J.K. (National Jeju Agricultural Experiment Station, R.D.A)
Seong, P.N. (National Jeju Agricultural Experiment Station, R.D.A)
Oh, W.Y. (National Jeju Agricultural Experiment Station, R.D.A)
Ko, M.S. (National Jeju Agricultural Experiment Station, R.D.A)
Kim, B.W. (Division of Applied Life Science, Gyeongsang National University)
Lee, J.G. (Division of Applied Life Science, Gyeongsang National University)
Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University)
Publication Information
Journal of Animal Science and Technology / v.46, no.1, 2004 , pp. 1-6 More about this Journal
Abstract
This study was conducted to investigate novel genetic variations of MCIR^*3 allele. In general, white spotting or white belt on a black backgroud in pigs is determined by the E$^p$ allele at the MCIR/Extention locus. E$^p$ shares a frameshift mutation with the E$^{D2}$ allele for dominant black color. An oligonucleotide primer set was designed to amplify complete coding sequence of the porcine MCIR gene. The MCIR coding sequences obtained from five breeds those were Landrace(white). Yorkshire(white), Hampshire(belt), Berkshire(spot) and Jeju native black pigs(black), were used for this study. A multiple sequence alignment of the MCIR coding region using Clustal W was performed. The total length of the MCIR coding sequence ranged from 963 to 966 base pairs(bp) among the selected breeds. The sequence analysis of the complete coding region of MCIR was revealed that Hampshire and Jeju native black pig have 3 cytosines deletion and Birkshire has 2 cytosines deletion at codon 23(nt68) in Extention loci. Besides the finding, there were three different missense mutations and a frameshift mutation in the MCIR coding region.
Keywords
MCIR; Extention; Novel genetic variation; Frameshift mutation; Missense mutation);
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