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Isolation and Structure Identification of Antibacterial Substances from the Rhizome of Zingiber mioga Roscoe  

Kim, Seong-Cheol (National Jeju Agricultural Experiment Station, R.D.A.)
Song, Eun-Young (National Jeju Agricultural Experiment Station, R.D.A.)
Kim, Kong-Ho (National Jeju Agricultural Experiment Station, R.D.A.)
Kwon, Hyeog-Mo (National Jeju Agricultural Experiment Station, R.D.A.)
Kang, Sang-Heon (National Jeju Agricultural Experiment Station, R.D.A.)
Park, Ki-Hun (Division of Applied Life Science, Gyeongsang National University)
Jung, Yong-Hwan (National Jeju Agricultural Experoment Station, R.D.A.)
Jang, Ki-Chang (National Jeju Agricultural Experiment Station, R.D.A.)
Publication Information
Applied Biological Chemistry / v.46, no.3, 2003 , pp. 246-250 More about this Journal
Abstract
In order to isolate antibacterial substances from the rhizome of Zingiber mioga Roscoe, the ethanol extracts was fractionated according to the activity against Bacillus subtilis, B. cereus and Staphylococcus aureus. Three antibacterial substances were isolated and purified by column chromatography and recrystallization. Compounds I and III showed activity against all the tested bacterias and compound II exhibited the activity against B. subtilis and B. cereus S. aureus. Compound I was examined antimicrobial activity against B. subtilis, B. cereus and S. aureus by optical density using Bioscreen C. Compound I showed strong growth inhibition at 10 ppm on B. subtilis and B. cereus for 72 hrs, and at 25 ppm on S. aureus. On the basis of spectrometric studies including $1^H-NMR$, ${13}^C-NMR$, DEPT, IH-lH COSY, HMQC, HMBC and IR, compounds I, II and III were identified as $(E)-8{\beta}(17)-epoxylabd-12-ene-15,16-dial\;(C_{20}H_{30}O_3,\;MW=318)$, galanolactone $(C_{20}H_{30}O_3\;MW=318)$ and galanal A $(C_{20}H_{30}O_3,\;MW=318)$, respectively. These results are the first reports on the isolation of $(E)-8{\beta}(17)-epoxylabd-12-ene-15,16-dial, galanolactone and galanal A from the rhizome of Zingiber mioga.
Keywords
Zingiber mioga Roscoe; antibacterial activity; labdane-type diterpene; $(E)-8{\beta}(17)-epoxylabd-12-ene-15,16-dial$; galanolactone; galanal A;
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