• Korean Journal of Medicinal Crop Science
• /
• v.13 no.5
• /
• pp.249-253
• /
• 2005

This study was carried out to develop convenient and reproducible methods for identifying the genetic relationship among germplasms of Panax species based on molecular genetics. Using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses, genetic polymorphism of the Panax species was investigated with following cultivars and accessions, such as Chunpoong, Yunpoong, Kopoong, Sunpoong, and Kumpoong in domestic cultivars, Hwangsuk, Jakyung and Suckju in domestic accessions, and Panax quinquefolius L. and Panax japonicus C.A. Meyer in foreign introduced accessions, respectively. Specific DNA fragments ranging from 200 to 3,000 base pairs in size could be obtained with various ISSR and RAPD primers under the optimized PCR conditions. The dissimilarity coefficients among the genetic polymorphisms of ginseng cultivars and accessions were calculated from 0.26 to 0.90 in RAPD and from 0.12 to 0.89 in ISSR analysis, respectively. Eleven plant samples were grouped siblings together with cultivars and parents based on cluster analysis of genetic distance depending on genetic property such as origin of the species. In results, both RAPD and ISSR analyses were useful for identifying the genetic relationship among cultivars and accessions of Panax species at DNA level.

• Animal cells and systems
• /
• v.11 no.2
• /
• pp.147-153
• /
• 2007

DNA extracted from ancient bones of Cervidae animals was examined to identify the species and to determine the phylogenetic relationships to those from extant cervids. Abundant ancient bones were excavated from Kumsung archaeological site in Jeju Island, Korea, and were identified as Cervidae animals based on morphological features of their antlers and lower mandibles. Their mitochondrial DNA (mtDNA) control region (CR) was partially sequenced and subsequently compared with those previously reported in database. The results confirmed that the ancient sequences are lineage of Cervidae. On the phylogenetic trees constructed using the sequence diversity of the CR sequences of family Cervidae, the ancient DNA sequences were found on distinct clusters. The ancient sequences were located in the subfamily Capreolinae cluster, and six ancient sequences were closely related to those of extant Korean roe deer in Jeju Island and Korean Peninsula. Consequently, the results of this study suggest that the roe deer inhabited Jeju Island in ancient times. However, there is no evidence for the existence of subfamily Cervinae, including Sika deer, while it has been described in several historical records. The results suggest that this finding could contribute to understanding of the origin and phylogenetic relationships of extant and ancient roe deer on Jeju Island.

• Advances in Traditional Medicine
• /
• v.7 no.1
• /
• pp.26-33
• /
• 2007

Genetic polymorphism and molecular authentication were investigated with the commercial medicinal herb, Peony (Paeonia spp.), using random amplified polymorphic DNA (RAPD) markers. To identify the polymorphism of the RAPD patterns among plant origins, 20 different random primers were applied to the genomic DNA extracted from Paeonia spp. plants such as Paeonia (P.) lactiflora, P. officinale and P. japonica. Ten primers out of 20 primers could be used to discriminate the plant species in the same genus and 72 out of 81 scored DNA fragments (88.9%) generated with these primers were polymorphic. Especially, four primers, such as OPA1, OPA3, OP9, and OPA13, were useful to discriminate the plant origins among the species of Peony. In the results of cluster analysis using RAPD data obtained from the 10 primers, Peony (Paeonia spp.) plants used in this study were grouped into the two distinctive clusters, genetically. Herb medicine, especially P. lactiflora, were easily identified, when species-specific primers were applied to the investigation for discriminating herb medicine currently traded in domestic herb market, Kyungdongmart. Consequently, RAPD analysis was useful method to discriminate plant origins and the commercial medicinal herbs, Paeonia spp..

• Journal of the Korean Home Economics Association
• /
• v.45 no.7
• /
• pp.105-117
• /
• 2007

The purposes of this study were 1) to classify adult consumers according to their desired clothing benefits on natural-dyed clothes, 2) to identify the differences of life-style among the classified groups, and 3) to determine the interest in Korean traditional culture, interest in dyeing, and demographics among the groups. The survey subjects were 283 adults. The data were analyzed utilizing factor analysis, cluster analysis, $x^2$-test, one-way ANOVA, Duncan test and multiple regression analysis. Based on the 6 dimensions of clothing benefits that were identified by factor analysis, adult consumers were classified into three clusters: eco-friendly oriented group showing the highest scores in environment conservation and comfort in clothing benefits, individuality-oriented group showing the highest scores in individuality and quality, and indifference group showing lower scores in all of the desired clothing benefits. The eco-friendly group showed the highest scores in attitude toward Korean traditional culture, and tended to have the most interest in natural and traditional methods of dyeing. The group members appeared to have passive life-styles, be older and married, and have a higher income than the other groups. The more positive attitude they had in Korean traditional culture, the higher was the purchase intention shown in the eco-friendly group. The individuality-oriented group showed active life-styles. This group tended to be younger than the other group. The more affirmative attitude in Korean traditional culture and the greater interest in the natural dyeing, the higher was the consumers' purchase intention of natural dyeing apparels. The indifference group tended to be younger, unmarried, and have a lower income. This group was not interested in natural dyeing apparels. The study findings demonstrated that consumers with different clothing benefits sought have different buying patterns on natural dyeing apparels. Finally the marketing implications are discussed.

• Journal of Korean Society of Rural Planning
• /
• v.19 no.3
• /
• pp.195-203
• /
• 2013

This study aimed to plan a distinctive apple theme park, thereby specializing the nationwide brand of Cheongsong apple. Detailed objectives included: to establish the best possible environments in Korea to taste and appreciate apple and enjoy the Cheongsong Apple Festival; to identify and foster natural, cultural and social resources in the clean environment of Cheongsong; to clusterize research and production infrastructures for strengthening local competitiveness; and to develop a hub for the vitalization of the region where visitors and locals can mutually prosper. The study was multi-phased. The first stage included basic surveys such as local status and environment analysis and similar case studies, and the second stage was to review the appropriateness of theme selection, develop basic principles and strategies for development goals and review and incorporate project details. And the third stage aimed to develop a comprehensive plan from spatial plans and program plans and suggest plans to vitalize the operation of the park. The dimension of the subject site was $180,150m^2$, which was divided into four areas, in consideration of the land use and the environmental characteristics of the resources, for developing a land use scheme. The four areas were named: the apple-theme cultual area; the agricultural culture experience area; the plaza for exchange and harmony; and the plaza for natural observation. This study has significance in that it can serve as a case to develop farm theme parks, and as a case of appropriate development of programs to identify amenity resources with a focus on the existing resources and in consideration of local characteristics.

• Journal of agricultural medicine and community health
• /
• v.18 no.2
• /
• pp.121-129
• /
• 1993

This study was conducted to investigate the incidence and related factors of farming tool-machinery injuries developed in the Chonnam rural area in 1992. A total of 9,068 persons (4,571men and 4,497women)were selected in a stratified cluster sampling manner, and interviewed individually to complete a structured questionnaire covering farmer characteristics, duration of farm working, annual income, kind of farming tools and machinery, type of injuries and medical care. The results were as follows : 1. The incidence rate of farming tool-machinery injuries was 63 per 1,000 persons over all, 73 in males and 54 in females. The age standardized incidence rate was 69 per 1,000 persons over all, 83 in males and 65 in females. 2. The associated factors with farming tool-machinery injuries in univariate analysis were sex, age, duration of farming and monthly income. The occurrence of injuries was higher in men than in woman, and the higher in high-income group than in the low-income group. 3. More men than woman were involved in trunk injuries and treated at the large scale medical care units such as hospitals, and they paid much higher medical fees. 4. The complaint rates for each item of farmers' syndrome in the injured group were higher than those of the non injured group in 'shoulder stiffness', 'sleeplessness', 'dizziness', and 'gastric fullness'. 5. When multiple logistic regression was applied to farming tool-machinery injuries, the significant variables were farmer's syndrome, age, and duration of farming in both males and females.

• Microbiology and Biotechnology Letters
• /
• v.43 no.1
• /
• pp.22-30
• /
• 2015

CSF is a major concern for the swine industry, representing currently the most epizootically dangerous disease to the species. Numerous CSFV isolates with various degrees of virulence have already been isolated worldwide, ranging from low virulent strains that do not result in any apparent clinical signs to highly virulent strains that cause a severe per acute hemorrhagic fever with very high mortality. The molecular epidemiology of CSFVs has proven to be an essential tool for effective disease control and the development of safe and effective vaccines. Therefore, this study cloned and sequenced local CSFV isolates, and conducted a phylogenetic analysis based on the E2 glycoprotein encoding sequences.The RNA was extracted from PK15 cell culture passaged CSFV isolates, the cDNA prepared, and the complete E2 gene amplified with a product size of 1186 bp. The gelpurified PCR product was cloned into a pGEMT easy vector and the positive clone commercially sequenced. Aligning the nucleotide (1119 bp) and amino acid (373) sequences with 29 reference strains revealed nucleotide and amino acid sequence identities of 82.60-97.80% and 88.70-98.70%, respectively, indicating a higher mutation rate of the field CSFV strains. The phylogenetic analysis based on the complete E2 amino acid sequences also revealed a reliable differentiation of all the analyzed strains into specific genetic groups and subgroups, plus the local isolate (CSFV-E2) was found to cluster with the CSFV subgroup 2.2. Thus, the full-length E2 cds proved to be most suitable for a reliable and statistically significant phylogenetic analysis of CSFV isolates.

• Korean Journal of Organic Agriculture
• /
• v.18 no.1
• /
• pp.83-92
• /
• 2010

Organic farming is a type of agricultural practices based on naturally occurring processes excluding or strictly limiting the use of synthetic fertilizers, pesticides, and other chemicals. This study was conducted to investigate the influences of agro-based materials, effective microorganisms (EM), liquid silicate (LS), and organic liquid fertilizer (OLF) for water dropwort (Oenanthe stolonifera DC.) cultivation. Soil pH, soil organic matter, and plant available phosphorous decreased with LS application. Exchangeable Ca and Mg decreased with EM application, and electrical conductivity and exchangeable Ca and K decreased with OLF application. Most of essential nutrient contents in water dropwort were reduced with the treatments of LS, EM, and OLF as compared with those in control plot, except nitrogen and phosphorus. However, diseases and insect pests were almost not observed in the water dropwort in the agro-based material application plots, except cluster caterpillar (Spodoptera litura). Productivity of water dropwort tended to be reduced: its higher productivity in the OLF and EM+LS plots and lower in the LS and control plots.

• The Korean Journal of Mycology
• /
• v.40 no.1
• /
• pp.11-18
• /
• 2012

This study was carried out to identify the genetic diversity of Penicillium isolates that were isolated from pears with postharvest decay in storage. URP-PCR was used to detect DNA diversity of 84 Penicillium isolates. Based on URP-PCR profiles, 18 Penicillium isolates were selected and their PCR polymorphic bands were produced by additional primers URP1F, URP2R, URP2F, and URP4R. UPGMA cluster analysis using the polymorphic bands showed four clustered groups and futhermore cultural and morphological features characterized the 18 Penicillium isolates. Group 1 was dominant, which occupies 70% in the four clustered groups and identified as P. expansum based on ITS sequence and morphological features.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.17 no.4
• /
• pp.264-272
• /
• 2016

In previous research, we analyzed traffic accident characteristics in the Chungcheong region through factor analysis, cluster analysis, and a questionnaire using traffic accident analysis system data to enhance Korea's traffic safety. Based on the analysis results, we investigated the design and application of traffic safety technology in non-urban areas in this study. Three technologies are proposed to improve traffic safety facilities for the region: a recognition light at pedestrian crossing works, a recognition light on the road for the underprivileged in traffic works, and a safety LED sign for operation of agricultural machine works. Each technology complements the light pollution problem about snow removal and road safety when applied to existing facilities in the non-urban areas. Solar-based indigenous technology is expected to contribute to road safety in rural areas.