• Mycobiology
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• v.46 no.1
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• pp.72-78
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• 2018

The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker "SCL-18" consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.

• Korean Journal of Agricultural Science
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• v.45 no.4
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• pp.889-903
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• 2018

The purposes of this study were to classify consumer types and to analyze purchase behavior depending on food safety attitudes related to the Fukushima nuclear accident. Based on the consumer survey data, factor analysis and cluster analysis were done to classify consumer types. Applying factor analysis, six factors with a total variance of 73.4% were extracted from 22 basic variables. The 6 factors were termed 'anxiety from ingesting agricultural and fishery products after the nuclear accident', 'importance of food safety when purchasing food', 'reduced rate of purchases of agricultural and fishery products after the accident', 'importance of food prices', 'socio-demographic characteristics' and 'family composition'. Using cluster analysis, consumers were classified into 5 types using the factor scores of 6 factors. The main results are as follows. For every consumer type, the anxiety about imported agricultural and fishery products was higher than that for domestic products after the nuclear accident. The food safety attitudes of consumers were mainly formed by the socio-demographic characteristics. Consumer types with high-education and high-income responded flexibly to the food safety concerning the accident; especially, the consumer type with a high-income and kids under 19 responded very reasonably to the accident. However, consumer types with a low-income and aged over 50 or less than 30 years were the least responsive to the accident, mainly due to financial restrictions. Thus, we suggest some food safety strategies and policy implications based on consumer type for food safety after the accident.

• Reproductive and Developmental Biology
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• v.38 no.2
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• pp.79-84
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• 2014

MicroRNAs (miRNAs) are approximately 22 nucleotides of small noncoding RNAs that control gene expression at the posttranscriptional level through translational inhibition and destabilization of their target mRNAs. The miRNAs are phylogenetically conserved and have been shown to be instrumental in a wide variety of key biological processes including cell cycle regulation, apoptosis, metabolism, imprinting, and differentiation. Recently, a paper has shown that expression of the miRNA-302/367 cluster expressed abundantly in mouse and human embryonic stem cells (ESCs) can directly reprogram mouse and human somatic cells to induced pluripotent stem cells (iPSCs) efficiently in the absence of any of the four factors, Oct4, Sox2, c-Myc, and Klf4. To apply this efficient method to porcine, we analyzed porcine genomic sequence containing predicted porcine miRNA-302/367 cluster through ENSEMBL database, generated a non-replicative episomal vector system including miRNA-302/367 cluster originated from porcine embryonic fibroblasts (PEF), and tried to make porcine iPSCs by transfection of the miRNA-302/367 cluster. Colonies expressing EGFP and forming compact shape were found, but they were not established as iPSC lines. Our data in this study show that pig miRNA-302/367 cluster could not satisfy requirement of PEF reprogramming conditions for pluripotency. To make pig iPSC lines by miRNA, further studies on the role of miRNAs in pluripotency and new trials of transfection with conventional reprogramming factors are needed.

• Journal of the Korean association of regional geographers
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• v.11 no.2
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• pp.233-246
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• 2005

This paper aims to explore the innovation milieu of red-pepper agro-industry in Bonghwa County, in the northern part of Gyeongbuk Province, and suggest policy recommendations for bringing up local agro-industry from a cluster perspective. It turns out that the capabilities of Bonghwa red-pepper agro-industry to innovate are remarkably weak let alone soft infrastructures such as learning networks between innovation agents and social capital. In this sense Bonghwa red-pepper agro-industry can be viewed as an agricultural area simply specialized in production sector rather than a kind of agro-industry cluster. For moving up Bonghwa red pepper industry into an innovative cluster, the paper suggests that the policy makers need to focus on cultivating local learning communities, building a region-wide incorporated brand, promoting the local agricultural technology centre as a network hub of cluster and fostering the functional links between universities and local innovation agents, covering farmers, agricultural organizations, agro-food producers and the local government.

• Journal of Agricultural Extension & Community Development
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• v.31 no.1
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• pp.1-11
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• 2024

This study segmented the market of senior rural tourists and analyzed their characteristics. A two-stage cluster analysis was used to analyze the types of senior rural tourists, and the difference in general characteristics and behavioral intention by segmented market was identified. In this study, data from the fact-finding survey on rural tourism 2022 were used, and 487 cases of senior with rural tourism experience in the past year were used for analysis. A total of three types of senior rural tourists were derived based on the motivation for rural tourism, annual expenditure for rural tourism, experience living in rural areas, and relationship with rural area. The segmentation market of senior rural tourists was found to be 'rural attachment type', 'rural curiosity type', and 'rural indifference type'. The results of this study suggest that the general characteristics and behavioral intentions of senior rural tourists differ according to the segment. The results of this study will be useful information in establishing effective marketing strategies targeting senior rural tourists.

• Journal of Korean Society of Rural Planning
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• v.23 no.3
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• pp.147-161
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• 2017

Climate changes from global warming and reduction in agricultural land result in volatility of prices of agricultural products, causing a imbalance of food market in Korea. It is necessary to develop a transnational food industry cooperation system among Korea, China and Russia that directly or indirectly affect food industry in terms of the whole industrial network. This study analyzes the value chain and linkage in the agriculture, forestry and fisheries industries in three nations. The unit structure and the industrial patterns of three nations were derived using the World Input-output Table (WIOT) from 2004 to 2014 every five years. This paper is expected to contribute to develop food security cooperation in the northern part of the Korean peninsula and to promote the mutual growth of food industry through industry linkage and cooperation.

• Korean Journal of Human Ecology
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• v.20 no.3
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• pp.677-689
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• 2011

The principal objective of this study was to evaluate the consumption behavior and perceptions of environmentally-friendly agricultural products (EAPs) according to the lifestyles of housewives in the Jeonbuk area, Korea. Self-administered questionnaires were collected from 267 housewives. Frequency analysis, chi-square, one-way ANOVA, factor analysis, and cluster analysis were used to analyze the data. Three clusters were obtained from the cluster analysis of LOHAS and wellbeing-related lifestyle: Cluster 1 "LOHAS-pursuit group", Cluster 2 "wellbeing-progress group", Cluster 3 "Utility-pursuit group". Of the housewives who were of LOHAS-pursuit group, about 50% were over 40 years old and had a professional job with a high household income. They had a high level of understanding about EAPs and purchased the highest percentage of EAPs among the groups. The housewives who were of the wellbeing-progress group, over 83% were between the ages of 30 and 40. Their consumption behaviors were very similar with that of the LOHAS-pursuit group, but the household income was lower. Of the housewives who were of the utility-pursuit group, about 63% under 30 years old. Their household income and level of understanding about EAPs were the lowest among the groups. They less interest in EAPs in comparison with other groups. For housewives' to choose EAPs properly, information and consumer education on these products, according to their lifestyles is necessary.

• Journal of Korean Society on Water Environment
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• v.37 no.6
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• pp.520-530
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• 2021

Multivariate statistical analysis and an environmental hydrological model were applied for investigating the causes of water pollution and providing best management practices for water quality improvement in urban and agricultural watersheds. Principal component analysis (PCA) and cluster analysis (CA) for water quality time series data show that chemical oxygen demand (COD), total organic carbon (TOC), suspended solids (SS) and total phosphorus (T-P) are classified as non-point source pollutants that are highly correlated with river discharge. Total nitrogen (T-N), which has no correlation with river discharge and inverse relationship with water temperature, behaves like a point source with slow and consistent release. Biochemical oxygen demand (BOD) shows intermediate characteristics between point and non-point source pollutants. The results of the PCA and CA for the spatial water quality data indicate that the cluster 1 of the watersheds was characterized as upstream watersheds with good water quality and high proportion of forest. The cluster 3 shows however indicates the most polluted watersheds with substantial discharge of BOD and nutrients from urban sewage, agricultural and industrial activities. The cluster 2 shows intermediate characteristics between the clusters 1 and 3. The results of hydrological simulation program-Fortran (HSPF) model simulation indicated that the seasonal patterns of BOD, T-N and T-P are affected substantially by agricultural and livestock farming activities, untreated wastewater, and environmental flow. The spatial analysis on the model results indicates that the highly-populated watersheds are the prior contributors to the water quality degradation of the river.

• The Korean Journal of Mycology
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• v.42 no.2
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• pp.159-164
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• 2014

For development of a method for differentiation of Pleurotus eryngii cultivars, simple sequence repeats (SSR) from whole genomic DNA sequence analysis was used for genotyping and two multiplex-SSR primer sets were developed. These SSR primer sets were employed to distinguish 12 cultivars and strains. Five polymorphic markers were selected based on the genotyping results. PCR using each primer produced one to four distinct bands ranging in size from 200 to 300 bp. Polymorphism information content (PIC) values of the five markers were in the range of 0.6627 to 0.6848 with an average of 0.6775. Unweighted pairgroup method with arithmetic mean clustering analysis based on genetic distances using five SSR markers classified 12 cultivars into two clusters. Cluster I and II were comprised of four and eight cultivars, respectively. Two multiplex sets, Multi-1 (SSR312 and SSR366) and Multi-2 (SSR178 and SSR277) completely discriminated 12 cultivars and strains with 21 alleles and a PIC value of 0.9090. These results might be useful in providing an efficient method for the identification of P. eryngii cultivars with separate PCR reactions.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.10a
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• pp.118-124
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• 2000

Zn-resistant Methylosobacterium strains were isolated from several non-polluted soil samples collected in all over Japan. Zn-resistant Methylosobacterium strains were predominantly detected in all soil samples and they were also characterized as a UV-tolerant bacteria. The MIC test revealed that the isolates have high zinc resistance in comparison with that of reference Methylosobacterium strains obtained from culture collections. The 16S rDNA-based phylogenetic analysis showed that all strains were divided into two clusters designated as cluster A and cluster B in the present study. All isolates were distributed only in the cluster A. The phylogenetic clustering also well coincided with the differences in the pattern of carbon source utilization.