• Mycobiology
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• 제46권1호
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• pp.72-78
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• 2018

The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker "SCL-18" consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.

• 농업과학연구
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• 제45권4호
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• pp.889-903
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• 2018

The purposes of this study were to classify consumer types and to analyze purchase behavior depending on food safety attitudes related to the Fukushima nuclear accident. Based on the consumer survey data, factor analysis and cluster analysis were done to classify consumer types. Applying factor analysis, six factors with a total variance of 73.4% were extracted from 22 basic variables. The 6 factors were termed 'anxiety from ingesting agricultural and fishery products after the nuclear accident', 'importance of food safety when purchasing food', 'reduced rate of purchases of agricultural and fishery products after the accident', 'importance of food prices', 'socio-demographic characteristics' and 'family composition'. Using cluster analysis, consumers were classified into 5 types using the factor scores of 6 factors. The main results are as follows. For every consumer type, the anxiety about imported agricultural and fishery products was higher than that for domestic products after the nuclear accident. The food safety attitudes of consumers were mainly formed by the socio-demographic characteristics. Consumer types with high-education and high-income responded flexibly to the food safety concerning the accident; especially, the consumer type with a high-income and kids under 19 responded very reasonably to the accident. However, consumer types with a low-income and aged over 50 or less than 30 years were the least responsive to the accident, mainly due to financial restrictions. Thus, we suggest some food safety strategies and policy implications based on consumer type for food safety after the accident.

• Reproductive and Developmental Biology
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• 제38권2호
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• pp.79-84
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• 2014

MicroRNAs (miRNAs) are approximately 22 nucleotides of small noncoding RNAs that control gene expression at the posttranscriptional level through translational inhibition and destabilization of their target mRNAs. The miRNAs are phylogenetically conserved and have been shown to be instrumental in a wide variety of key biological processes including cell cycle regulation, apoptosis, metabolism, imprinting, and differentiation. Recently, a paper has shown that expression of the miRNA-302/367 cluster expressed abundantly in mouse and human embryonic stem cells (ESCs) can directly reprogram mouse and human somatic cells to induced pluripotent stem cells (iPSCs) efficiently in the absence of any of the four factors, Oct4, Sox2, c-Myc, and Klf4. To apply this efficient method to porcine, we analyzed porcine genomic sequence containing predicted porcine miRNA-302/367 cluster through ENSEMBL database, generated a non-replicative episomal vector system including miRNA-302/367 cluster originated from porcine embryonic fibroblasts (PEF), and tried to make porcine iPSCs by transfection of the miRNA-302/367 cluster. Colonies expressing EGFP and forming compact shape were found, but they were not established as iPSC lines. Our data in this study show that pig miRNA-302/367 cluster could not satisfy requirement of PEF reprogramming conditions for pluripotency. To make pig iPSC lines by miRNA, further studies on the role of miRNAs in pluripotency and new trials of transfection with conventional reprogramming factors are needed.

• 한국지역지리학회지
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• 제11권2호
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• pp.233-246
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• 2005

본 연구의 목적은 우리나라의 대표적인 낙후지역이자 고추주산지의 하나인 경상북도 봉화군의 고추 농산업의 혁신환경을 분석하고, 이를 토대로 지역 고추 농산업의 육성 전락을 클러스터 관점에 기초하여 제시하는 것이다. 봉화군 고추농산업의 혁신인프라는 매우 취약하고 산학연관 네트워크 또한 활성화되어 있지 못하므로, 클러스터 발전단계 상으로 단순 고추 주산지 상태에 머무르고 있는 것으로 나타났다. 그러나 지식커뮤니티의 결성 및 지자체의 적극적인 산학연관 네트워크 육성 의지 둥에 따라 혁신환경이 점진적으로 개선되는 변화양상도 확인된다. 이를 토대로 본 연구에서는 봉화 고추 농산업을 클러스터화를 위해서는 학습 커뮤니티 조직의 활성화, 공동 브랜드 구축, 네트워크 매개기관으로서의 농업기술센터의 역할 제고, 지식창출의 중심 연계기관으로서 지역 대학과의 연계 강화 등에 초점을 두어야 함을 제안한다.

• 농촌지도와개발
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• 제31권1호
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• pp.1-11
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• 2024

This study segmented the market of senior rural tourists and analyzed their characteristics. A two-stage cluster analysis was used to analyze the types of senior rural tourists, and the difference in general characteristics and behavioral intention by segmented market was identified. In this study, data from the fact-finding survey on rural tourism 2022 were used, and 487 cases of senior with rural tourism experience in the past year were used for analysis. A total of three types of senior rural tourists were derived based on the motivation for rural tourism, annual expenditure for rural tourism, experience living in rural areas, and relationship with rural area. The segmentation market of senior rural tourists was found to be 'rural attachment type', 'rural curiosity type', and 'rural indifference type'. The results of this study suggest that the general characteristics and behavioral intentions of senior rural tourists differ according to the segment. The results of this study will be useful information in establishing effective marketing strategies targeting senior rural tourists.

• 농촌계획
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• 제23권3호
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• pp.147-161
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• 2017

Climate changes from global warming and reduction in agricultural land result in volatility of prices of agricultural products, causing a imbalance of food market in Korea. It is necessary to develop a transnational food industry cooperation system among Korea, China and Russia that directly or indirectly affect food industry in terms of the whole industrial network. This study analyzes the value chain and linkage in the agriculture, forestry and fisheries industries in three nations. The unit structure and the industrial patterns of three nations were derived using the World Input-output Table (WIOT) from 2004 to 2014 every five years. This paper is expected to contribute to develop food security cooperation in the northern part of the Korean peninsula and to promote the mutual growth of food industry through industry linkage and cooperation.

• 한국생활과학회지
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• 제20권3호
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• pp.677-689
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• 2011

The principal objective of this study was to evaluate the consumption behavior and perceptions of environmentally-friendly agricultural products (EAPs) according to the lifestyles of housewives in the Jeonbuk area, Korea. Self-administered questionnaires were collected from 267 housewives. Frequency analysis, chi-square, one-way ANOVA, factor analysis, and cluster analysis were used to analyze the data. Three clusters were obtained from the cluster analysis of LOHAS and wellbeing-related lifestyle: Cluster 1 "LOHAS-pursuit group", Cluster 2 "wellbeing-progress group", Cluster 3 "Utility-pursuit group". Of the housewives who were of LOHAS-pursuit group, about 50% were over 40 years old and had a professional job with a high household income. They had a high level of understanding about EAPs and purchased the highest percentage of EAPs among the groups. The housewives who were of the wellbeing-progress group, over 83% were between the ages of 30 and 40. Their consumption behaviors were very similar with that of the LOHAS-pursuit group, but the household income was lower. Of the housewives who were of the utility-pursuit group, about 63% under 30 years old. Their household income and level of understanding about EAPs were the lowest among the groups. They less interest in EAPs in comparison with other groups. For housewives' to choose EAPs properly, information and consumer education on these products, according to their lifestyles is necessary.

• 한국물환경학회지
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• 제37권6호
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• pp.520-530
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• 2021

Multivariate statistical analysis and an environmental hydrological model were applied for investigating the causes of water pollution and providing best management practices for water quality improvement in urban and agricultural watersheds. Principal component analysis (PCA) and cluster analysis (CA) for water quality time series data show that chemical oxygen demand (COD), total organic carbon (TOC), suspended solids (SS) and total phosphorus (T-P) are classified as non-point source pollutants that are highly correlated with river discharge. Total nitrogen (T-N), which has no correlation with river discharge and inverse relationship with water temperature, behaves like a point source with slow and consistent release. Biochemical oxygen demand (BOD) shows intermediate characteristics between point and non-point source pollutants. The results of the PCA and CA for the spatial water quality data indicate that the cluster 1 of the watersheds was characterized as upstream watersheds with good water quality and high proportion of forest. The cluster 3 shows however indicates the most polluted watersheds with substantial discharge of BOD and nutrients from urban sewage, agricultural and industrial activities. The cluster 2 shows intermediate characteristics between the clusters 1 and 3. The results of hydrological simulation program-Fortran (HSPF) model simulation indicated that the seasonal patterns of BOD, T-N and T-P are affected substantially by agricultural and livestock farming activities, untreated wastewater, and environmental flow. The spatial analysis on the model results indicates that the highly-populated watersheds are the prior contributors to the water quality degradation of the river.

• 한국균학회지
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• 제42권2호
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• pp.159-164
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• 2014

큰느타리 품종구분을 위한 마커의 개발을 위하여 큰느타리 전체 유전자 염기서열을 바탕으로 제작한 484개의 SSR마커를 사용하여 다형성 분석을 실시하였다. 그 결과 각 275개의 primer에서 다형성이 관찰되었다. 이 중 품종간에 다양한 패턴을 나타내는 5개의 마커를 최종 선발하였다. 이들 마커의 PIC 값은 0.6627에서 0.6848로 나타났고, 평균값은 0.6775였다. 이 결과를 밴드 이미지 인식 방법으로 dendrogram을 작성하였다. UPGMA 집괴분석 결과, 큰느타리 품종은 크게 Cluster 1과 Cluster 2로 구분되었다. SSR primer를 이용한 PCR 결과 나타나는 품종별 고유의 DNA 밴드를 품종특이적 마커로 개발하기 위하여, 선발된 마커중에서 SSR312과 SSR366, SSR178과 SSR 277 마커를 조합하여 초위성체 유래 다중 표지 세트를 개발하였다. Multiplex-SSR 마커의 사용을 통해 두번의 PCR 반응만으로 본 연구에서 사용된 12개의 큰느타리 품종을 구분할 수 있었다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2000년도 추계 학술대회
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• pp.118-124
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• 2000

Zn-resistant Methylosobacterium strains were isolated from several non-polluted soil samples collected in all over Japan. Zn-resistant Methylosobacterium strains were predominantly detected in all soil samples and they were also characterized as a UV-tolerant bacteria. The MIC test revealed that the isolates have high zinc resistance in comparison with that of reference Methylosobacterium strains obtained from culture collections. The 16S rDNA-based phylogenetic analysis showed that all strains were divided into two clusters designated as cluster A and cluster B in the present study. All isolates were distributed only in the cluster A. The phylogenetic clustering also well coincided with the differences in the pattern of carbon source utilization.