• 한국자원식물학회지
• /
• 제19권2호
• /
• pp.199-203
• /
• 2006

콩나물의 상품성은 형태에 따라 크게 변한다. 본 연구는 상면살수 방식에서 재배통의 흔들음 정도가 콩나물의 생장과 형태에 미치는 영향을 조사하고자 재배 초기 3일간의 흔들음 횟수(0, 3, 5회/일)와 기간(5회/일로 1, 2, 3일)을 달리하여 시험을 수행하였던 바 그 결과를 요약하면 다음과 같다. 1. 개체당 형성된 세근수는 흔들음 횟수와 기간이 증가할수록 적어지는 경향을 보였다. 2. 콩나물 전체길이는 흔들음 횟수와 기간간에 차이가 없었다. 그러나 흔들음 횟수와 기간이 많고 길수록 하배축은 짧아졌던 반면, 뿌리는 길어지는 것으로 조사되었다. 3. 자엽 바로 아래의 hook 직경은 흔들음 횟수가 증가할수록 커졌으나, 하배축 중간부분의 직경은 흔들음 횟수와 기간간에 차이가 없는 것으로 나타났다. 4. 개체당 각부위 및 전체 생체중과 건물중은 흔들음 횟수와 기간간에 차이가 없었다. 따라서 상면살수 방식에서 재배통의 흔들음은 콩나물의 생장보다는 형태에 주로 영향을 미치는 것으로 나타났다.

• KSBB Journal
• /
• 제26권4호
• /
• pp.357-364
• /
• 2011

This study was carried out to investigate the effects of agitation, aeration and scale-up on the mycelial growth, exo-polysaccharide (EPS) production, and mycelial morphology in the liquid culture of Ganoderma lucidum. A correlation between roughness and operating variables was also studied to scale-up the liquid culture of G. lucidum in a jar fermenter. When the agitation speed or aeration rate increased, the morphological form was changed from rough pellet to smooth pellet form. Increase of the agitation and aeration reduced the mycelial roughness. On the other hand, in the case of pellet size, it was not affected by aeration. The higher EPS production was obtained at approximately 17% of roughness and mycelial pellet size of 3~5 mm. The morphology at each fermenter was closely correlated with kLa value, and it was found that similarity of morphology would be used as a criteria of scale-up for liquid culture of G. lucidum.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제9권2호
• /
• pp.173-181
• /
• 2004

To delay the onset of apoptosis in the culture, transformed Tn 5B1-4 cells harboring anti-apoptotic genes, bcl-2 and baculovirus p35, have been established and analyzed for their anti-apoptotic ability in suspension culture using spinner flasks. In the suspension culture at agitation speeds of 100 rpm and 200 rpm, the cell growth of cell clone expressing Bcl-2 protein was much higher than other two clones and the maximum cell density of the clone was 6.0 ${\times}$ 10$^{6}$ cells/ml and 6.2 ${\times}$ 10$^{6}$ cells/ml at day three of the incubation. On the other hand, the cell growth of cell clone expressing baculovirus protein P35 was much higher than other two clones in suspension culture at agitation speed of 300 rpm and the maximum cell density of the clone was 6.1 ${\times}$ 10$^{6}$ cells/ml at day three of the incubation. Based on the pattern of genomic DNA laddering and the microscopic observation of apoptotic bodies, the more apoptotic bodies are induced in Tn 5B1-4 control cell clone at higher agitation speed. This result shows that the shear stress can be a main factor in inducing apoptosis in spinner flask culture. At low agitation speed, cell clone expressing Bcl-2 was more effective in delaying the onset of apoptosis than the cell clone expressing P35. On the other hand, at high agitation speed, cell clones expressing baculovirus P35 was more effective in delaying the onset of apoptosis than the cell clone expressing Bcl-2. Therefore, anti-apoptotic genes, bcl-2 and baculovirus p35, can playa distinct role depending on agitation speed in the suspension culture.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제10권6호
• /
• pp.571-575
• /
• 2005

A simple, but effective on-line method for estimating the mycelial cell mass concentration from agitation speed data, a most readily-available process variable, has been developed for DO-stat cultures of Agaricus blazei. The dynamic change of dissolved oxygen concentration (DOC) in the initial transient period and the change in yield were considered in the development of the estimation algorithm or estimator. Parameters in the estimation algorithm were calculated from the agitation speed data at 20% of DOC. The proposed estimator could accurately predict the cell mass concentration regardless of DOC levels in the tested range of $10{\sim}40%$, showing a good extrapolation capability.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2001년도 추계학술발표대회
• /
• pp.382-385
• /
• 2001

The effect of agitation speed on the production of mycelial growth and exo-biopolymer in bioreaetor culture was investigated. The maximum production of mycelia and exo-biopolymer was obtained at 350 rpm, where the maximum concentrations of mycelial growth and exo-biopolymer were 62 g/L and 23 g/L, respectively. The effect of agitation speed on the hyphal length and the number of tips was examined. The hyphal length and the number of tips of Cordyceps sinensis 16 in bioreaetor culture at 350 rpm were increased up to 660 ${\mu}m$ and 14/ml, respectively.

• 산업기술연구
• /
• 제24권B호
• /
• pp.171-176
• /
• 2004

The effects of agitation and aeration for exopolysaccharide(EPS) production through batch cultivation of an Enterobacter sp. isolated from the composter were investigated. During the EPS fermentation under conditions of constant agitation speed from 200 to 900 rpm and constant aeration rate of 0.5-2.5 vvm, the low yields of EPS(4.8-5.2g/L) was observed as the viscosity increase of culture broth. With the stepwise increases in agitation speed and aeration rate, the EPS production and the viscosity of EPS were increased 1.3~1.4 times and 2.3~3.6 times higher than those of the fixed conditions, respectively. Therefore, these stepwise increases were considered as the key operating parameters for enhancing EPS production. The max. EPS(6.8g/L) and viscosity(14,000cp) were obtained when the agitation speed was increased from 300 to 900 rpm for 54hrs at 1.5 vvm.

• Journal of Plant Biotechnology
• /
• 제38권1호
• /
• pp.30-41
• /
• 2011

본 연구에서는 고추의 나출 소포자 배양 시 배양 중 새배지의 첨가와 진탕이 배의 생산에 미치는 영향을 조사하였다. 액체-2층 배양에서 초기 액체배양 시에 새 배지를 첨가하면 배의 발생과 발달 모두 크게 증가하였다. 가장 효과적인 첨가 시기는 초기액체 배양을 시작한 5일 후 이었다. 한편 액체-2층 배양에서 후기 2층배양 시의 새 배지 첨가는 초기 액체배양 시 첨가 때에 비해 그 효과가 적었다. 액체-2층 배양에서 후기 2층배양 시의 1주간 진탕은 정상 자엽배 생산에 효과적이었다. 액체배양시에도 배양 1주 후의 1주간 진탕은 배의 발달에 효과적 이었다. 그러나 액체-2층배양 시와 액체배양 시 모두 진탕기간이 2 ~ 3 주간으로 길어질 때에는 배의 발달이 비정상적이었다. 본 실험 결과 얻어진 정상 자엽배들은 재분화 배지에 이식 시 용이하게 유식물체로 발달하였다. 재분화 식물체들 중에는 반수체와 배가반수체가 혼재하였으며, 이들 간에는 공변세포 내 엽록체 수의 차이가 뚜렷하였다. 이와 같은 결과들은 고추에서 다수의 정상자엽배를 생산할 수 있는 소포자 배양 시스템을 확립하는 데 중요한 기초자료가 될 것이다.

• Journal of Microbiology and Biotechnology
• /
• 제10권5호
• /
• pp.628-631
• /
• 2000

The timing of poly(3-Hydroxybutyrate) (PHB) biosynthesis was controlled by varying the agitation speed of a stirred tank fermentor during the pH-stat fed-batch culture of recombinant Escherichia coli strain GCSC 6576 harboring pSYL107. Using a concentrated whey solution containing ca. 200 g/l lactose as the nutrient feed, the PHB content was only 57% after 35h due to volumetric limitation of the fermentor. However, by limiting the oxygen by maintaining the agitation speed at 300 rpm, the final PHB content increased to 70% after 70h with a cell concentration of 15 g/l. When the agitation speed was increased up to 500 rpm, a cell concentration of 31 g/l with 80% PHB was obtained after 52h. A further increase in the maximum agitation speed increased the cell concentration, PHB concentration, and PHB productivity, however, the PHB content decreased to 56-58%.

• 한국버섯학회지
• /
• 제19권2호
• /
• pp.81-87
• /
• 2021

Roridin E, produced by Podostroma cornu-damae, is a mycotoxin with anticancer activity. To increase the content of roridin E, submerged culture conditions were optimized using response surface methodology. Three factors, namely, medium initial pH, incubation time and agitation speed were optimized using a Box-Behnken design. The optimum submerged culture conditions to increase the content of roridin E included a medium with an initial pH of 4.0, an incubation time of 12.90 days, and an agitation speed of 63.03 rpm. The roridin E content in the submerged culture, under the aforementioned conditions, was 40.26 mg/L. The findings of this study can help lower the current price of roridin E and promote its related research.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
• /
• pp.350-351
• /
• 2000

We isolated cellulose-producing bacteria from persimmon vinegar(Korea traditional fermentation food). Some of these strains were selected for cellulose production in agitation culture. On the other hand, it was also found that strains suitable for static culture production were not necessarily suitable for agitation culture. Therefore we estimated the cellulose production of these isolates in static culture. To determine nutritional requirement for the production of bacterial cellulose, several nutrients as carbon source, nitrogen and mineral salt were tested.