• 한국수산과학회지
• /
• 제56권6호
• /
• pp.833-840
• /
• 2023

In this study, the antioxidant and anti-skin aging properties of the Korean marine algae Ishige foliacea were investigated. Solvent extracts from I. foliacea were prepared with 70% ethanol, 80% methanol, and water. The extraction yields of various solvent extracts ranged from 9.55% to 35.12%. In terms of antioxidant activity, the ethanol extract showed the highest ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging activity, nitrite oxide scavenging activity, reducing power, and FRAP (ferric reducing antioxidant power). Regarding anti-skin aging activity, evaluation of the skin whitening and anti-wrinkle activities revealed that the methanol, water, and ethanol extracts possessed the highest tyrosinase (IC₅₀=0.98 mg/mL), elastase (IC₅₀=0.15 mg/mL), and collagenase (IC₅₀=0.06 mg/mL) inhibitory activities, respectively. These results suggest that I. foliacea holds potential as an antioxidant and anti-skin aging substance in food and cosmetic materials.

• 대한화장품학회지
• /
• 제42권3호
• /
• pp.303-309
• /
• 2016

연령이 증가함에 따라 피부 내부에서는 콜라겐의 합성 능력이 떨어지고 탄력섬유의 손상 및 matrix metalloproteinase (MMPs) 발현이 증가한다. 본 연구의 목적은 눈가에 주름이 형성되기 전에 연령이 증가할수록 피부 내부에서 피부 노화의 징후가 관찰된다는 것을 확인하고, 피부 내부에서 먼저 발생하는 노화의 징후가 칠엽담 지페노사이드 분획물 함유 크림(칠엽담 지페노사이드 크림)의 효능으로 인해 개선되는 것을 확인하는데 있다. 이에 본 연구에서는 육안으로 눈가에 주름이 관찰되지 않는 21 ~ 34세의 한국 여성 52명을 대상으로 임상 시험을 수행하였다. 이들에게서 연령이 증가함에 따라 피부 외부의 노화 징후가 관찰되지는 않지만 피부 내부에서 노화의 징후가 시작되는 시점을 진피치밀도 측정을 통해 확인하였다. 그리고 육안으로 주름이 관찰되지는 않았지만, 진피치밀도가 감소하는 연령대에서 칠엽담 지페노사이드 크림을 4주 및 8주 사용한 후에 진피치밀도 값이 유의하게 증가함을 확인하였다. 따라서 칠엽담 지페노사이드 크림이 피부 내부에서 먼저 진행되는 노화를 효과적으로 개선하는데 도움을 주며, 이 결과를 토대로 칠엽담 지페노사이드 분획물이 향후 주름 개선기능성 화장품 개발에 활용하기 적합하다고 판단된다.

• Journal of Applied Biological Chemistry
• /
• 제59권3호
• /
• pp.239-242
• /
• 2016

A novel Nicotinoyl fused peptide, Nicotinoyl-LVH, was synthesized by solid phase peptide synthesis method, purified, and tested in cultured skin cells. Nicotinoyl-LVH enhanced the expression level of collagen mRNA and its fragments in fibroblasts. These data show that this novel Nicotinoyl peptide is a promising biomaterial in the anti-aging functional cosmetic market.

• Environmental Analysis Health and Toxicology
• /
• 제23권2호
• /
• pp.129-138
• /
• 2008

The purpose of this study was to investigate the effects of frankincense oil in skin aging animal model. Skin aging was induced by both the irradiation of UVB and the application of squalene monohydroperoxide (Sq-OOH) to the back of experimental animals for 4 weeks. And at the same time experimental materials were applied topically. Six to seven weeks female SHR-1 hairless mice were divided into five groups including normal (N: saline), control (C: UVB+Sq-OOH+saline), vehicle control (VC: UVB+Sq-OOH+jojoba oil), positive control (PC: UVB+Sq-OOH+0.01% retinoic acid) and experimental (E: UVB+Sq-OOH+3% Frankincense oil) groups, five animals each group. Lipid lamella and lipid content in stratum corneum of the E group were almost intact with a regular arrangement which were similar to the N group. Collagen fibers in dermis of the E group were almost intact with a regular arrangement which were similar to the N group. Relatively much less number of mast cells and inflammatory cells were found in the E group compared to the C group. The activities of XO, SOD and CAT were no significant difference between the E and N groups. In conclusion, the application of frankincense oil to the skin aging animal model reduced both the generation of free radicals and the damage of skin tissues. Therefore, frankincense oil can be used practically for the prevention or improvement of skin aging in terms of health promotion and beauty for the people.

• Genomics & Informatics
• /
• 제3권2호
• /
• pp.66-72
• /
• 2005

The epidermis is a physiological barrier to protect organisms against environment. During the aging process, skin tissues undergo various changes including morphological and functional changes. The transcriptional regulation of genes is part of cellular reaction of aging process. In order to examine the changes of gene expression during the aging process, we used the primary cell culture system of human keratinocytes. Since UV radiation is the most important environmental skin aggressor, causing skin cancer and other problems including premature skin aging, we examined the changes of gene expression in human keratinocytes after UV irradiation using oligonucleotide microarray containing over 10,000 genes. We also compared the gene expression patterns of the senescent and UV treated cells. Expression of the variety of genes related to transcription factors, cell cycle regulation, immune response was altered in human keratinocytes. Some of down-regulated genes are represented in both senescent and UV treated cells. The results may provide a new view of gene expression following UVB exposure and aging process in human keratinocytes.

• KSBB Journal
• /
• 제31권3호
• /
• pp.178-185
• /
• 2016

Aging of the face is mainly related to the features that are sagging or loss of elasticity of the skin by reducing the volume around the eyes or cheek. Intrinsic aging can be seen to cause thinner dermis, reduction of extracellular matrix and subcutaneous fat. This study was carried out to investigate the skin volume augmentation and anti-wrinkle effects of Tribulus terrestris fruit extract. Skin anti-aging effect of Tribulus terrestris fruit extract was evaluated by using lipid accumulation, expressin of type I procollagen and elastin in preadipocytes and human dermal fibroblasts. Tribulus terrestris fruit extract augmented preadipocytes differentiation about 56% at 100 µg/mL. The type I procollagen and elastin were increased about 35% and 25% by treatment 20% Tribulus terrestris fruit extract, respectively. The clinical study also showed that skin sagging, skin elasticity, and dermal density improved without adverse effect following 4 week application of cream containing 2% Tribulus terrestris fruit extract. We suggest that Tribulus terrestris fruit extract can have the good possibility as skin volume augmenting, skin elasticity and wrinkle improving agent.

• 대한화장품학회지
• /
• 제30권3호
• /
• pp.385-388
• /
• 2004

최근에 천연성분의 화장품 응용에 대한 관심이 높아지고 식물에 함유하고 있는 피토에스트로겐(Phytoestrogen)의 항노화 물질 연구가 중요해지고 있다. 태국 식물 Pueraria mirifica 추출물을 $4\%$ 함유한 크림제제(PM 크림)를 추출물 성분이 제외된 플라시보 크림과 비교하여 총 30명 여성을 대상으로 16주간 임상시험을 수행하였다. 피부 모사판을 제작하여 주름 측정을 하였고, 피부 탄력과 보습을 기기 평가하였다. 특히 피부 탄력도는 placebo 크림 사용군 대비 추출물 함유 크림 사용군이 통계적으로 유의하게 탄력도가 증가하였다. 주름이나 보습평가는 특이할 만한 결과를 나타내지는 못했다. Pueraria mirifiaca는 피부 탄력 개선을 주는 항노화 화장품 개발에 응용될 수 있을 것이라 기대된다.

• 대한화장품학회지
• /
• 제41권2호
• /
• pp.135-141
• /
• 2015

세포 노쇠화(cell senescence)는 나이 듦에 따른 내인성 노화 및 질병들에서 나타날 수 있는 세포의 노화인자 발현, 세포분열 정지 등의 현상으로 일컬어진다. 피부세포의 경우, 노화 및 외부요인으로 인한 세포 노쇠화가 일어나 세포분열의 정지 및 기능 이상이 관찰되며 이는 피부노화를 가속화시키는 요인이 된다. 본 연구에서는, cordycepin을 이용하여 노화된 피부세포의 세포 노쇠화 억제 및 기능 향상을 유도하여 피부노화 개선의 가능성을 제시하였다. 사람에서 유래한 섬유아세포를 이용하여 세포의 ${\beta}$-galactosidase 활성 세포염색 결과, 많은 계대의 세포에서 발현이 높게 나타남을 알 수 있었다. 항산화 및 항염 효과가 알려진 cordycepin을 많은 계대의 세포에 처리하였을 때 ${\beta}$-galactosidase 활성이 확연히 떨어짐을 확인하였고 무혈청 배지 조건에서 많은 계대 세포의 증식 및 생존율을 높이는 결과를 보였으며 세포 노쇠화와 많은 연관성이 대두되고 있는 미토콘드리아의 기능관련 실험을 진행한 결과, 높은 ROS억제능이 나타났다. 본 연구를 통하여 노화된 사람 피부 섬유아세포에서의 cordycepin의 세포 노화 개선능을 알 수 있었으며, 피부 항노화소재로서의 가능성을 확인하였다.

• 대한본초학회지
• /
• 제22권4호
• /
• pp.219-225
• /
• 2007

Objectives : We investigated the anti-aging effects on skin with Saccharomyces fermented modified Kyungohkgo extract (SFKE). Methods : Fermented modified Kyungohkgo extracts by 14 different kinds of microorganism were prepared and most effective Saccharomyces fermented modified Kyungohkgo extract among these was selected for anti-aging agent. We measured various effects related to skin such as scavenging activity against free radical, cell proliferation, collagen synthesis and toxicity of SFKE were evaluated and compared with modified Kyungohkgo extract (KE). Results : Free-radical scavenging activity and cell proliferating ratio of SFKE were 2 and 8 times higher than those of KE. The SFKE could significantly increase the collagen synthetic ratio compared with KE treated group. SFKE showed no toxicity at all tested concentrations. Conclusions : The results of our study propose that SFKE have good anti-aging effects on skin.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제45권1호
• /
• pp.1-11
• /
• 2022

Silkworm pupae, a by-product of the silk industry are known to be valuable resource of nutrients for humans as well as animals besides encompassing diverse bioactive constituents. However, there is a paucity of knowledge on their role in amelioration of oxidative stress and anti-skin aging properties. In the present study, we evaluated the inhibitory effect of aqueous as well as ethanolic (30% and 70%) extracts from distinct stages of male and female silkworm pupae belonging to two silkworm varieties on skin aging-related enzymes. The activities of collagenase, elastase and tyrosinase were effectively inhibited by 70% ethanolic silkworm pupal extracts (SPE), followed by 30% with aqueous extracts exhibiting meager inhibitory potential. SPE were also investigated for their antioxidant activity in oxidative-stressed murine fibroblasts (L929). The intracellular ROS and lipid peroxidation induced by tert-butyl hydroperoxide (t-BHP) in fibroblasts was better attenuated by pre treatment with ethanolic (30%) and aqueous extracts, respectively. The safety of the extracts was determined by studying their effect on fibroblast cell viability and it was found that none of the extracts were cytotoxic. Our findings indicate the potential utility of SPE as anti-aging components in cosmeceuticals.