• 청정기술
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• 제9권1호
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• pp.9-21
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• 2003

CFC-113, 1,1,1-TCE는 오존파괴물질로 선진국에서는 이미 사용이 전면 규제되고 있고 MC, TCE는 유해성 또는 발암성물질로 판명되어 일부사업장에서 제한적으로 사용되고 있다. 그러므로 세정성이 좋고 환경/안전성이 우수한 대체세정제를 개발하여 사용하거나 기존 개발된 세정제 중에서 우수한 세정제를 선정하여 사용하는 것이 필요한 실정이다. 대체세정제로는 수계세정제가 환경성과 경제적면에서 유망한 것으로 평가받고 있어 많은 사업장에서 사용될 전망이다. 본 연구에서는 환경친화적인 수계세정제를 개발하기 위하여 수계세정제의 주요성분인 여러 형태의 계면활성제를 선정하여 이들의 물성, 거품성, 오염물 종류에 따른 세정성, 유수분리성을 평가하여 비교하였다.

• 식물병연구
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• 제26권1호
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• pp.48-52
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• 2020

2019년 7월 안동지역 노지 재배지에서 복숭아에 과실 썩음병 피해가 발생하였다. 피해 과실에서는 병이 진전됨에 따라, 썩음증상과 함께, 흰색과 자주색을 띄는 균사 및 포자가 관찰되었다. 병원균을 순수 분리한 뒤, 건전한 복숭아 과일에 접종하였을 때 동일한 과실 썩음 증상을 유도하였다. 분리된 병원균의 internal transcribed spacer (ITS), translation elongation factor 1-alpha (TEF) 그리고 β-tubulin (β-TUB) sequence 분석을 통해 Fusarium avenaceum으로 동정되었다. 따라서, 이 증상을 Fusarium avenaceum에 의한 "복숭아 과실 썩음병"으로 명명하고자 한다. 분리된 균주는 농촌진흥청 국립농업과학원 미생물 은행[Korean Agricultural Culture Collection(KACC)]에 기탁되었다(KACC accession number 48936).

• Biomolecules & Therapeutics
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• 제18권1호
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• pp.106-110
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• 2010

The pharmacokinetics and bioavailability of ambroxol, an expectoration improver and mucolytic agent, were studied to determine the feasibility of enhanced transdermal delivery of ambroxol from the ethylene-vinyl acetate (EVA) matrix system containing polyoxyethylene-2-oleyl ether as an enhancer in rats. The ambroxol-010 matrix system (15 mg/kg) was applied to abdominal skin of rats. Blood samples were collected via the femoral artery for 28 hrs and the plasma concentrations of ambroxol were determined by HPLC. Pharmacokinetic parameters were calculated using Lagran method computer program. The area under the curve (AUC) was significantly higher in the enhancer group ($1,678{\pm}1,413.3\;ng/ml{\cdot}hr$) than that in the control group $1,112{\pm}279\;ng/ml{\cdot}hr$), that is treated transdermally without enhancer, showing about 151% increased bioavailability (p<0.05). The average $C_{max}$ was increased in the enhancer group ($86.0{\pm}21.5\;ng$/ml) compared with the control group ($59.0{\pm}14.8\;ng$/ml). The absolute bioavailability was 13.9% in the transdermal control group, 21.1% in the transdermal enhancer group and 18.1% in the oral administration group compared with the IV group. The $T_{max}$, $K_a$, MRT and $t_{1/2}$ of ambroxol in transdermal enhancer group were increased significantly (p<0.01) compared to those of oral administration. As the ambroxol-EVA matrix containing polyoxyethylene-2-oleyl ether and tributyl citrate was administered to rats via the transdermal routes, the relative bioavailability increased about 1.51-fold compared to the control group, showing a relatively constant, sustained blood concentration. The results of this study show that ambroxol-EVA matrix could be developed as a transdermal delivery system providing sustained plasma concentration.

• Journal of the Korean Wood Science and Technology
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• 제42권5호
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• pp.543-554
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• 2014

Natural plant extract has been the subject of intense research aiming in elucidating the underlying mechanisms of their chemopreventive effects upon various forms of human cancers. The objective of our study was to evaluate the natural antioxidants and anticancer agent potential of Phyllostachys. The chemical composition of steam extract from Phyllostachys was carried out using GC-MS. The steam extract of Phyllostachys was dominated by monoterpenes (62.96% - 71.36%) and sesquiterpenes (23.58% - 33.13%) as the main compounds. The antioxidant activities of the steam extract was determined using a DPPH scavenging and hydrogen peroxide scavenging activity test systems. Furthermore, the amounts of total phenolics in steam extract were determined spectrometrically The steam extract of P. pubescens and P. bambusoides were presented the high activity (69.4% and 64.0%, respectively.). The steam extract from Pyllostachys species showed a hydrogen peroxide scavenging activity of approximately 50.4% - 54.6% when compared to that of the standard gallic acid. The anticancer activities of steam extract were determined using a MTT assay. Assessment of the cytotoxic effect of the steam extract on PC-3 cells showed that the P. bambusoides (20.85%) and P. pubescens (20.41%) were superior in induced cytotoxicity compared with the steam extract of P. nigra var. henonis (1.15%). Findings from this study indicated that steam extract of P. bambusoides and P. pubescens possessed potential as medicinal drug especially in prostate cancer treatment.

• 한국임상약학회지
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• 제28권1호
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• pp.24-29
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• 2018

Background: Cyclosporine is an immunosuppressive agent used to treat and prevent graft versus host reaction (GVHR)-a complication associated with stem cell transplantation. This study aimed to develop a population pharmacokinetic model of cyclosporine and investigate factors affecting cyclosporine clearance in pediatric hematopoietic stem cell transplant patients. Methods: A total of 650 cyclosporine concentrations recorded in 65 patients who underwent hematopoietic stem cell transplantation were used. Data including age, sex, weight, height, body surface area (BSA), type of disease, chemotherapy before stem cell transplantation, type of donor, serum creatinine levels, total bilirubin concentration, hematocrit value, and type of concomitant anti-fungal agents and methylprednisolone used were retrospectively collected. Data related to cyclosporine dosage, administration time, and blood concentration were also collected. All data were analyzed using the non-linear mixed effect model; a two-compartment model with first-order elimination was used. Results: The population pharmacokinetic model of cyclosporine using the NONMEM program was as follows: $CL(L/h)=5.9{\times}(BSA/1.2)^{0.9}$, V2 (L) = 54.5, Q (L/h) = 3.5, V3 (L) = 1080.0, $k_a(h^{-1})=0.000377$. BSA was selected as a covariate of cyclosporine clearance, which increased with an increase in BSA. Conclusion: A population pharmacokinetic model for Korean pediatric hematopoietic stem cell transplant patients was developed, and the important factor affecting cyclosporine clearance was found to be BSA. The model might contribute to the development of the most appropriate dosing regimen for cyclosporine. Further studies on population pharmacokinetics should be carried out, prospectively targeting pediatric patients.

• Journal of Microbiology and Biotechnology
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• 제28권10호
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• pp.1645-1653
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• 2018

The genus Acer contains several species with various bioactivities including antioxidant, antitumor and anti-inflammatory properties. However, Acer okamotoanum Nakai, one species within this genus has not been fully studied yet. Therefore, in this study, we investigated the anti-adipogenic activities of leaf extract from A. okamotoanum Nakai (LEAO) on 3T3-L1 preadipocytes. Adipogenesis is one of the cell differentiation processes, which converts preadipocytes into mature adipocytes. Nowadays, inhibition of adipogenesis is considered as an effective strategy in the field of anti-obesity research. In this study, we observed that LEAO decreased the accumulation of lipid droplets during adipogenesis and down-regulated the expression of key adipogenic transcription factors such as peroxisome proliferator-activated receptor ${\gamma}$ (PPAR ${\gamma}$) and CCAAT/enhancer binding protein ${\alpha}$ (C/EBP ${\alpha}$). In addition, LEAO inactivated PI3K/Akt signaling and its downstream factors that promote adipogenesis by inducing the expression of PPAR ${\gamma}$. LEAO also activated ${\beta}$-catenin signaling, which prevents the adipogenic program by suppressing the expression of PPAR ${\gamma}$. Therefore, we found that treatment with LEAO is effective for attenuating adipogenesis in 3T3-L1 cells. Consequently, these findings suggest that LEAO has the potential to be used as a therapeutic agent for preventing obesity.

• The Plant Pathology Journal
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• 제30권3호
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• pp.288-298
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• 2014

We examined the efficacy of a bacterium for biocontrol of the root-knot nematode (RKN) Meloidogyne hapla in carrot (Daucus carota subsp. sativus) and tomato (Solanum lycopersicum). Among 542 bacterial isolates from various soils and plants, the highest nematode mortality was observed for treatments with isolate C1-7, which was identified as Bacillus cereus based on cultural and morphological characteristics, the Biolog program, and 16S rRNA sequencing analyses. The population density and the nematicidal activity of B. cereus C1-7 remained high until the end of culture in brain heart infusion broth, suggesting that it may have sustainable biocontrol potential. In pot experiments, the biocontrol efficacy of B. cereus C1-7 was high, showing complete inhibition of root gall or egg mass formation by RKN in carrot and tomato plants, and subsequently reducing RKN damage and suppressing nematode population growth, respectively. Light microscopy of RKN-infected carrot root tissues treated with C1-7 showed reduced formation of gall cells and fully developed giant cells, while extensive gall cells and fully mature giant cells with prominent cell wall ingrowths formed in the untreated control plants infected with RKNs. These histopathological characteristics may be the result of residual or systemic biocontrol activity of the bacterium, which may coincide with the biocontrol efficacies of nematodes in pots. These results suggest that B. cereus C1-7 can be used as a biocontrol agent for M. hapla.

• 한국콘텐츠학회논문지
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• 제13권10호
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• pp.189-199
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• 2013

이 연구는 무용사회화 주관자가 무용전공생의 무용참가 및 무용성취에 미치는 영향을 규명하는데 목적이 있다. 이 연구는 2013년 현재 4년제 대학 무용학과에 재학 중인 무용전공생들을 모집단으로 선정한 다음, 유의표집법(Purposive sampling)을 이용하여 265명을 표집 하였다. 타당도 및 신뢰도는 요인분석 및 신뢰도분석을 통해 검증하였으며, 신뢰도는 계수는 .779이상으로 나타났다. 자료처리는 SPSSWIN 18.0 프로그램을 활용하여 요인분석, 신뢰도분석, 회귀분석 등이 활용되었다. 결론은 다음과 같다. 첫째, 무용사회화 주관자인 부친, 모친, 형제자매, 친구, 학교교사는 무용전공생의 무용참가에 영향을 미친다. 둘째, 무용사회화 주관자인 부친, 모친, 형제자매, 친구, 학교교사는 무용성취에 영향을 미친다. 셋째, 무용전공생의 무용참가는 무용성취에 영향을 미친다.

• 한국추진공학회지
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• 제22권3호
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• pp.90-96
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• 2018

고체 점화제 $BKNO_3$는 항공우주 및 유도탄 등의 점화제로 널리 적용되는데 본 연구에서는 $BKNO_3$의 제조공정과 특성평가를 수행하였다. 이 고체 점화제는 금속분말과 산화제 그리고 유기 고분자물질(결합재)로 구성된다. 증발법(evaporation process)을 사용하여 원료들을 균질하게 혼합하였다. CEA 프로그램을 사용하여 $BKNO_3$의 물질특성 및 열적 반응 해석을 통한 최적 조성비를 설계하였으며, 입도, 형상, 감도, 열량 분석 등의 특성 평가를 수행하여 결과를 분석하였다.

• 산업경영시스템학회지
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• 제41권1호
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• pp.118-127
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• 2018

Recently, service quality must reflect several demands of customers who show rapid and various changes so as to be compared with the past. So, objective and rapid measuring methods for service quality are necessary. For them, first of all, service company must calculate their standard of service quality accurately by measuring service quality exactly. Kano classified the degree of influence that is the degree of correspondence of the quality attributes of products and services to the subjective satisfaction of customers. As a result, the types of qualities are classified as attractive, must be, one dimensional, and indifference attributes. They have been widely used quality attributes in various industrial fields up to now. However, Kano model has a limit that it ignores the characters of the next frequent numbers even though there are not much gap comparing to the most frequent number in the questionnaire answers. The limit is attributed to the character of Kano model that the most frequent number is accepted as the only quality character. Timko calculated the customer satisfaction coefficient by using Kano's method and studied the differences in quality character by classifying the quality characteristics in a graphical way through the relationship between the satisfaction and the dissatisfaction coefficient. In this study, we used the quality level determination method of the 7-point Likert scale, which takes the weight into account, to complement the deficiencies of the existing Kano model. We also developed and applied a Potential Satisfaction Level (P) and Potential Customer Demand Improvement (PCDI) Index to present a new approach to the determination of service quality attributes. To measure the level of potential service satisfaction and to understand the degree of improvement, we collected specimens of 51 participants who has been trained in the National Strategy Business Training Program, which has been managed by government agent, and analyzed the results.