• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2008년도 International Meeting of the Microbiological Society of Korea
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• pp.171-171
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• 2008

We monitored the occurrence of human enteric viruses in urban rivers by cell culture-PCR and RT-nested PCR. Water samples were collected monthly or semimonthly between May 2002 and March 2003 in four urban tributaries. Enteric viruses were detected by RT-nested PCR and cell culture-PCR based on a combination of Buffalo Green monkey kidney (BGMK) and A549 cell lines, followed by phylogenetic analysis of amplicons. By RT-nested PCR analysis, 45 (77.6%), 32 (55.2%), 32 (55.2%), 26 (44.8%), 12 (20.7%), 2 (3.4%), 4 (6.9%), and 4 (6.9%) of 58 samples showed positive results with adenoviruses, enteroviruses, noroviruses (NV) genogroup I (GI) and II (GII), reoviruses, hepatitis A viruses, rotaviruses and sapoviruses, respectively. Adenoviruses were most often detected and only eight (13.8%) samples were negative for adenoviruses and positive for other enteric viruses in the studied sites. Thirty-one (77.5%) of the 40 samples were positive for infectious adenoviruses and/or enteroviruses based on cell culture-PCR, and the frequency of positive samples grown on A549 and BGMK (65.0%) was higher than that grown on BGMK alone (47.5%). The occurrence of each enteric virus, except reoviruses and hepatitis A viruses was not statistically correlated with the water temperature and levels of fecal coliforms according to Binary logistic regression model. By sequence analysis, most strains of adenoviruses and enteroviruses detected in this study are similar to the causative agent of viral diseases in Korea and most NV GI- and GII-grouped strains were closely related to the reference strains from China and Japan, and GII/4-related strains had similar sequences to strains recognized as a worldwide epidemic outbreak. Our results suggested that monitoring human enteric viruses is necessary to improve microbial quality and cell culture-PCR using the combination of A549 and BGMK cells and the adenovirus detection by PCR could be useful for monitoring viral contamination in the aquatic environment.

• 대한약침학회지
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• 제15권2호
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• pp.7-10
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• 2012

Objectives: The root of Lithospermum erythrorhizon Sieb. et Zucc. (Lithospermi Radix, LR) is a kind of heat clearing and blood cooling medicinal herbs. It can clear away heat and cool the blood, reduce toxins and disperse maculae. LR has long been used as efficacious therapy for inflammation, burns, frostbite and skin diseases such as eczema and psoriasis. Methods: In the present study, we investigate anti-allergic and anti-inflammatory effects of LR by using the 1-fluoro-2, 4- dinitrofluorobenzene (DNFB)-induced contact dermatitis mouse model. Results: Topical application of 10 mg/mL of LR effectively inhibited skin lesions induced by repeated paintings with DNFB. Topical application of LR also inhibited hyperplasia, edema, spongiosis and infiltrations of mononuclear cells. In addition, production levels of total immunoglobulin and IgG1 in serum were decreased by using LR in vivo. Conclusions: These data suggest that LR acts as an antiinflammatory agent, improving skin lesions in CD mice.

• 인터넷정보학회논문지
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• 제14권3호
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• pp.67-79
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• 2013

무선 네트워크에서 효율적인 이동성관리는 모바일 사용자의 지원에 상당히 중요하다. 몇몇의 이동성관리 기법들은 MN(Mobile Node)의 시그널링 트래픽을 줄여주는 목적으로 제안되었다. 그 중에서 PMIPv6(Proxy Mobile IPv6)는 호스트 기반의 이동성관리 프로토콜과 유사하지만 MN에게 이동성에 대한 어떠한 처리도 요구하지 않는다. MAG(Mobile Access Gateway)와 LMA(Local Mobility Anchor)같은 새로운 모바일 에이전트를 도입하여 MN에게 IP 이동성을 제공한다. 본 논문에서, MN 이동성의 종류에 따라 호출 당 평균 시그널링 지연과 평균 대역폭을 평가하는 분석 모델을 제안한다. 수학적 분석의 결과로 MF-PMIP(Multicasting-based FastPMIP)는 거의 모든 성능평가 매개변수에 대하여 D-PMIP에 비해 우수한 성능을 보여준다.

• Molecules and Cells
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• 제22권1호
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• pp.104-112
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• 2006

We previously reported that DA-9601, ethanol herbal extract of Artemisia asiatica, inhibited histamine and leukotriene releases in guinea pig lung mast cells activated with specific antigen/antibody reaction. This study aimed to evaluate the inhibitory effect of DA-9601 on the OVA-induced airway inflammation in allergic asthma mouse model. BALB/c mice were sensitized and challenged with OVA. DA-9601 was administered orally 1 h before every local OVA-challenge. OVA-specific serum IgE was measured by ELISA, recruitment of inflammatory cells in BAL fluids and lung tissues by Diff-Quik and H&E staining, respectively, the expressions of CD40, CD40L and VCAM-1 by immunohistochemistry, goblet cell hyperplasia by PAS staining, activities of MMPs by gelatin zymography, expressions of mRNA and proteins of cytokines by RT-PCR and ELISA, activities of MAP kinases by western blot, and activity of NF-${\kappa}B$ by EMSA. DA-9601 reduced IgE level, recruitment of inflammatory cells into the BAL fluid and lung tissues, expressions of CD40, CD40L and VCAM-1 molecules, goblet cell hyperplasia, MMPs activity, expressions of mRNA and productions of various cytokines, activities of MAP kinases and NK-${\kappa}B$ increased from OVA-challenged mice. These data suggest that DA-9601 may be developed as a clinical therapeutic agent in allergic diseases due to suppressing the airway allergic inflammation via regulation of various cellular molecules expressed by MAP kinases/NF-${\kappa}B$ pathway.

• Preventive Nutrition and Food Science
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• 제18권2호
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• pp.104-110
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• 2013

Presence of Helicobacter pylori is associated with an increased risk of developing upper gastrointestinal tract diseases. Antibiotic therapy and a combination of two or three drugs have been widely used to eradicate H. pylori infections. Due to antibiotic resistant drugs, new drug resources are needed such as plants which contain antibacterial compounds. The aim of this study was to investigate the ability of GutGard$^{TM}$ to inhibit H. pylori growth both in Mongolian gerbils and C57BL/6 mouse models. Male Mongolian gerbils were infected with the bacteria by intragastric inoculation ($2{\times}10^9$ CFU/gerbil) 3 times over 5 days and then orally treated once daily 6 times/week for 8 weeks with 15, 30 and 60 mg/kg GutGard$^{TM}$. After the final administration, biopsy samples of the gastric mucosa were assayed for bacterial identification via urease, catalase and ELISA assays as well as immunohistochemistry (IHC). In the Mongolian gerbil model, IHC and ELISA assays revealed that GutGard$^{TM}$ inhibited H. pylori colonization in gastric mucosa in a dose dependent manner. The anti-H. pylori effects of GutGard$^{TM}$ in H. pylori-infected C57BL/6 mice were also examined. We found that treatment with 25 mg/kg GutGard$^{TM}$ significantly reduced H. pylori colonization in mice gastric mucosa. Our results suggest that GutGard$^{TM}$ may be useful as an agent to prevent H. pylori infection.

• Human Ecology Research
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• 제53권1호
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• pp.93-106
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• 2015

The purpose of this study is to understand what we have learnt thus far on the sustainable consumption of natural resources by household in Korea. We propose a two-wing model designating households as the main agent of sustainable living where both the production of human resources and the consumption of natural resources take place. In the current study, we focused on the sustainability of natural resource consumption. Undertaking an empirical study, we selected 10 major journals that represent each area of human ecological research and then, identified 120 relevant studies regarding sustainability published since 2000. First, we classified 381 keywords into three categories: production, consumption, and social environment, and the identified studies were then classified in one of the seven categories created by combinations of the abovementioned three categories: production (P), consumption (C), social environment (S), production-consumption (PC), production-social environment (PS), consumption-social environment (CS), and production-consumption-social environment (PCS). The results showed that the sustainability of natural resources has been mainly investigated in the fields of clothing & textiles and food & nutrition. Further, consumer science research has been conducted in the area of consumption-social environment and consumption-production. The current study indicates that the field of human ecology has created and accumulated knowledge to support sustainable living. In the future, we need to conduct more integrated research across disciplines and methods and promote research interest in the underdeveloped area of sustainability research in human ecology.

• 한국통신학회논문지
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• 제36권2A호
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• pp.149-159
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• 2011

최근 모바일 컴퓨팅 환경은 사회 전반으로 보편화가 진행되고 있다. 이러한 경향은 모바일 플랫폼 상에서 무선 네트워트를 통한 유선 네트워크 상의 데이터베이스 시스템의 접근 필요성을 부각시킨다. 그러나 모바일 컴퓨팅 환경은 전통적인 컴퓨팅 환경과는 본질적으로 다른 특성으로 인하여 데이터베이스 접근 방식을 그대로 적용하기 어렵다. 이 논문은 새로운 에이전트 기반 모바일 데이터베이스 접근 모델을 제시하고, 데이터 비축을 위한 캐시 교체와 일관성 유지 과정에서 대상 데이터를 선정하는 두 가지 데이터 중요도 함수를 제안한다. 이 함수들은 데이터의 접근 기간, 접근 빈도, 접근 경향, 갱신 빈도, 갱신 경향, 데이터 크기 분포 등을 종합적으로 반영한다. 모의실험에 의하면, 제안하는 함수를 사용한 정책은 LRU 정책, LIX 정책, SAIU 정책에 비하여 접근 지연 시간 감소, 캐시 바이트 적중률 향상, 캐시 바이트 오염률 감소 측면에서 경쟁력이 있음을 보인다.

• Bulletin of the Korean Chemical Society
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• 제31권11호
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• pp.3333-3340
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• 2010

A three dimensional pharmacophore model was generated for the molecules which are responsible for anti-inflammatory activities targeting Interleukin-2 inducible tyrosine kinase (Itk). 16 structurally diverse molecules were selected as training set to generate the hypotheses using Discovery Studio v2.1. The best hypothesis, Hypo1, comprises two hydrogen bond acceptor (HBA), one hydrophobic aromatic (HA), one ring aromatic (RA) and shows high cost difference (63.71), high correlation coefficient (0.97) as well as low RMS deviation (0.81). Hypo1 has been further validated toward a test set, decoy set and Fischer's randomization method. Furthermore, Hypo1 was used to screen NCI and Maybridge databases. Finally, 2 hit molecules were identified as potential leads against Itk, which may be useful for future drug development.

• 디지털콘텐츠학회 논문지
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• 제11권1호
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• pp.47-55
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• 2010

문화콘텐츠 산업은 지식기반 핵심산업으로, 정보통신기술에 인간의 창의성, 상상력, 감성, 스타일을 투입하여 문화콘텐츠기반 경제사회로 전환하고 있다. 문화산업은 문화전쟁이라는 커다란 변화속에 콘텐츠파워(contents power)로 급속히 변화하고 있으며, 이러한 변화의 추세속에서 우리 문화콘텐츠는 아시아를 넘어 각국으로 진출을 시도하고 있다. 그러나 현재 제한된 현지시장정보와 지역특성을 반영한 문화전반에 대한 이해 부족으로 많은 어려움을 겪고 있다. 이에 문화콘텐츠 글로벌 네트워크 구축을 통하여 문화콘텐츠의 지속적인 수출확대와 신규시장개척의 교두보 역할을 수행할 수 있는 문화콘텐츠 지역전문가 양성이 요청된다. 본 논문에서는 진출 대상 국가의 문화산업 리딩그룹을 국내에 초청하여 우리나라 콘텐츠를 경험케 함으로써 자국 내에서 친 한류 지속화 기반 및 수출확대를 지원하는 초청연수 및 한국기업의 해당국 파견연수, 한국내 체류하는 유학생 연수에 대한 교육지원시스템, ETS-CS(Educational Training System for Contents Specialist)을 설계하고 이를 현장에서 적용한 사례를 분석하여 향후 더 나은 시스템으로 활용도를 높이고자 한다.

• Restorative Dentistry and Endodontics
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• 제17권2호
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• pp.355-364
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• 1992

This is a study on the effect of the dentinal pretreatment method to the bond strength between dentin and glass ionomer cement. In this study, 196 human molar teeth with sound crown were used. The dentin surfaces of these teeth were exposed with wet trimmer and polished with # 800 Emory paper and teeth were divided into 7 groups according to the pretreatment agent and method. Each group has 4 subroups of the kinds of glass ionomers. The shear bond strength were measured by Instron Universal Testing machine model 1122. The data of the evaluations were then subjected to statistical analysis using one way ANOVA and the result were as follows : 1. In Durelon liquid 20 sec scrubbing & Vitrebond filling subgroup, shear bond strength was highest with measurements of 72.41(kg/$cm^2$) and in no pretreatment & Shofu lining cement filling subgroup, lowest with measurements of 4.77(kg/$cm^2$). 2. In no pretreatment group, statistical significant differences were found between the subgroups of G-C lining cement and Shofu lining cement. 3. In Ketac conditioner 20 sec scrubbing group, Vitrebond were bonded stronger than others, and in Ketac conditioner 10 sec passive contact group, it has the significant difference with other glass ionomers except G-C lining cement. 4. The subgroup og G-C dentin conditioner 20 sec scrubbing & G-C lining cement filling was bonded to dentin stronger than the subgroup of no pretreatment & Shofu lining cement. In G-C dentin conditioner groups, both of 10 sec passive contact and 20 sec scrubbing, Vitrebond has highest bond strength among the subgroups. 5. The subgroup of Durelon liquid 10 sec passive contact & G-C lining cement filling was bonded to dentin stronger than the subgroup of no pretreatment & Shofu lining cement. Also in both Durelon liquid groups, Vitrebond were bonded to dentin with the highest strength among the subgroups.