• Journal of Acupuncture Research
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• v.32 no.4
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• pp.109-118
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• 2015

Objectives : The purpose of this study was to investigate the anti-oxidative effect of Suhwagije-decoction (SGT) pharmacopuncture solution on the liver and spleen cells of aged rats. Methods : The liver and spleen cells of 10, 52, and 72 week old Sprague Dawley (SD) rats were divided into three groups: distilled water (DW), Vit.C, and SGT groups, which were treated with DW, Vit.C and SGT pharmacopuncture solution respectively. The levels of superoxide dismutase (SOD), glutathione (GSH) and nitric oxide (NO) were then measured. Results : 1. Treatment of SGT pharmacopuncture solution significantly decreased NO concentration in spleen cells of 52 week old rats as compared with that of other groups. 2. Treatment of SGT pharmacopuncture solution significantly increased SOD activity in spleen cells of 72 week old rats as compared with that of other groups. Conclusions : Suhwagije-decoction (SGT) pharmacopuncture solution may have an anti-oxidative effect in the spleen cells of aged rats.

• Journal of Physiology & Pathology in Korean Medicine
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• v.30 no.3
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• pp.157-163
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• 2016

The purpose of this study is to investigate the anti-oxidative effect of Yongyukjowi-tang (YJT) distillate on liver and spleen cells of aged rats. This experiment was performed using liver and spleen cells of 10w, 52w, 72w old SD rats. The cells were divided into three groups; DW, Vit.C, and YJT groups, which were treated with distilled water, vitamin C, and YJT distillate respectively, and the levels of Superoxide Dismutase (SOD) and malondialdehyde (MDA) were measured. SOD level in liver and spleen cells of 10w old rats was significantly increased in YJT group compared to DW group. MDA level in liver cells of 72w old rats was significantly decreased in YJT group compared to DW group. Yongyukjowi-tang distillate showed an anti-oxidative effect on liver and spleen cells of aged rats.

• Journal of Sasang Constitutional Medicine
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• v.23 no.3
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• pp.374-390
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• 2011

1. Objectives: The purpose of this study is to investigate the effect of Suhwagije-tang(SGT) distillate on the immune activity of spleen cells of aged SD rats. 2. Methods: We used 10, 50, 72 weeks old SD rats in this study. Spleen cells from SD rats were stimulated with ConA and treated with 1% Vitamin C(Vit.C) or Suhwagijetang distillate(SGT). After 24 hours, the concentrations of IL-2, IL-4, IL-10, IFN-${\gamma}$ in the cell culture supernatant were measured by ELISA. 3. Results and Conclusions 1) At all concentration of SGT distillate, survival rates of liver cells were higher than the control group. In addition, 50% SGT distillate group's cell survival rates were significantly higher than other groups. 2) In 10 weeks SD rats(SGT group), the concentration of IL-2 significantly decreased in comparison with ConA group, Vit.C group. In 52 weeks SD rats(SGT group), the concentration of IL-2 significantly decreased in comparison with ConA group. 3) In 10, 52 weeks SD rats(SGT group), the concentration of IL-4 significantly decreased in comparison with ConA group. 4) In 10 weeks SD rats(SGT group), the concentration of IL-10 significantly decreased in comparison with ConA group. And in 72 weeks SD rats(SGT group), the concentration of IL-10 significantly increased in comparison with Vit.C group. 5) In 52, 72 weeks SD rats(SGT group), the concentration of IFN-${\gamma}$ significantly decreased in comparison with 10 weeks SD rats(SGT group). These results suggest that Suhwagije-tang(SGT) distillate has the effect of increasing the immune activity of spleen cells of aged SD rats.

• The Korean Journal of Food And Nutrition
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• v.9 no.1
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• pp.1-11
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• 1996

Male rats of 2 months-old(young), 6 months-old(adult) and 30 months-old(old)were fed 8 or 16 weeks to investigate the e(feats of dietary fat level on the lipid metabolism in the different stages of aging. The response of 30 month old rats to dietary fat level differed from either 2 or 6-months old rats. In 30-months old rats, dietary fat level had a little effect on weight gains, lipid content of serum as compared with other two age groups. Young rats showed higher body weight gain, F.S.R, and PER compared with aged rat. The younger and the more high fat fed the rats were, the more body weight gains, FER and PER showed. Weight of liver and kidney was increased in aged rats but ratio per unit weight of liver and kidney was not affected by age. Serum lipid content was higher in aged rat compared with the young and liver lipid content was higher in high fat diet fed rats. But TG of liver and serum was not changed with aging process. Intake, excretion and absorption of lipid was not changed by age of animals. but absorption was decreased as experimental period was increased.

• Journal of Sasang Constitutional Medicine
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• v.19 no.3
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• pp.227-241
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• 2007

1. Objectives The purpose of this study is to investigate the anti-oxidative and immune regulatory effect of oral administration of Chungsimyeonja-tang decoction in aged rats. 2. Methods The rats in the normal group were 14 weeks old, and those in the control and Chungsimyeonja-tang groups were 40 weeks old. The rats in Chungsimyeonja-tang group were orally treated with chungsimyeonjatang decoction daily for 2 months. To observe the anti-oxidative effect and immune regulatory effect of Chungsimyeonja-tang, we performed blood chemistry analysis, histological analysis. 3. Results and Conclusions 1) The number of WBC in blood was significantly increased and the number of PLT in blood was significantly decreased by oral administration of Chungsimyeonja-tang decoction. 2) The levels of albumin, LDL cholesterol, ALP and GOT in serum were significantly decreased, and the level of glucose in serum was significantly increased by oral administration of Chungsimyeonja-tang decoction. 3) The concentrations of glutathione and catalase were significantly increased and the concentrations of NO and MDA were significantly decreased in the liver of early aged rats by oral administration of Chungsimyeonja-tang decoction. 4) The concentrations of SOD and catalase were significantly increased and the concentration of MDA was significantly decreased in the lung of aged rats by oral administration of Chungsimyeonja-tang decoction. 5) The levels of IL-4 and IL-6 were significantly decreased while the levels of IFN-${\gamma}$ were significantly increased in the spleen of early aged rats by oral administration of Chungsimyeonja-tang decoction.

• The korean journal of orthodontics
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• v.21 no.3
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• pp.521-541
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• 1991

The purpose of this study was to investigate the growth changes of the mandible and associated structure in response to postural hyperpropulsion and changes after removal of postural hyperpropulsor. The experimental animals were four-week-old Sprague-Dawley males rats. The animals were worn the postural hyperpropulsor diurnally for 10 hours per day. The animals were sacrified after 1-week, 2-week, 4-week postural hyperpropulsion and 4-week postural hyperpropulsion 4-week removal period. The growth changes of rat mandible and associated structures following postural hyperpropulsion on the growing rat mandible were observed biometrically, radiographically and histologically. The finding were as follows. 1. The angle between the chief axis of the bone trabeculae in the condyle and the mandibular plane of rats observed for 4 weeks after worn the hyperpropulsor for 4 weeks was directed posteriorly as compared with that of control rats. 2. The ratio of mandibular length to maxillary length of experimental rats was higher than that of control rats. 3. The tooth axis of mandibular first molar of rats worn the postural hyperpropulsor for 4 weeks was mesially inclined as compared with control rats. 4. Histologically, the cartilage layer at the superior region of the condyle of rats worn the postural hyperpropulsor for 2 weeks appeared thicker than that of same aged normal rats, and generalized increase of the cartilage layer was shown on the condyle of rats worn the postural hyperpropulsor for 4 weeks. 5. There was no significant histologic difference between rats observed for 4 weeks after worn the postural hyperpropulsor for 4 weeks (8 week experimental rats) and same aged normal rats. 6. The newly formed bone at anterior region of articular fossa of rats worn the postural hyperpropulsor for 2 weeks and 4 weeks was thicker than that of same aged normal rats.

• The Journal of Korean Medicine
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• v.37 no.1
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• pp.90-100
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• 2016

Objectives: The purpose of this study was to investigate the effect of Jeokbaekhaogwanjung-tang (JGT) distillate on the immune activity of spleen cells of aged rats. Methods: Spleen cells of 10w, 52w 72w old SD rats were cultured and treated with JGT distillate and the levels of IL-2, IL-4, IL-10 and IFN-${\gamma}$ were measured. Results: 1. The levels of IL-2 and IFN-${\gamma}$ in the spleen cells of 52w old rats were significantly decreased by JGT treatment. 2. The level of IFN-${\gamma}$ in the spleen cells of 72w old rats were significantly decreased by JGT treatment. Conclusion: These results suggest that Jeokbaekhaogwanjung-tang distillate has an immune regulative effect by way of suppressing the change of immune activity caused by aging in rats.

• The Korea Journal of Herbology
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• v.31 no.4
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• pp.35-41
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• 2016

Objective : The purpose of this study is to investigate the anti-oxidative effect of Jeokbaekhaogwanjoong-tang (JGT) distillate in spleen cells of aged rats.Methods : This experiment was performed using spleen cells of 10w, 52w, 72w old sprague dawley (SD) rats. The spleen cells of 10, 52, 72 weeks old rats were divided into three groups; DW group, Vit. C group, and JGT group. The cells of DW group were treated with distilled water. The cells of Vit.C group were treated with Vitamin C. And the cells of JGT group were treated with JGT distillate. After the treatment of DW, Vit.C, and JGT, the levels of superoxide dismutase (SOD), glutathione (GSH), nitric oxide (NO) and lipid peroxidation (MDA) were measured.Results : In the spleen cells of 72 weeks old rats, SOD activity was significantly increased in the JGT group compared to those in the DW group and the Vit.C group. In the spleen cells of 52 weeks old rats, GSH activity was significantly increased in the JGT group compared to the Vit.C group, and NO concentration was significantly decreased in the JGT group compared to the DW group and the Vit.C group. MDA concentration was not changed significantly in any cell groups by the treatment of JGT distillate.Conclusions : Jeokbaekhaogwanjoong-tang (JGT) distillate may have an anti-oxidative effects in the spleen cells of aged rats. Further investigation is needed for the anti-aging effect of JGT.

• Biomedical Science Letters
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• v.13 no.1
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• pp.55-60
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• 2007

Computerized image processing and analysis system was used for quantitative assessment of skin tissue components in color histological sections. The purpose of this study was to determine that the changes in the collagen fiber density and elastic fiber density in dermis in the rat skin as aging. And also to determine that the correlation between fibroblast density and collagen fiber density and elastic fiber density in the aged rat skin. Ten weeks old ($130{\sim}150g$) eight and fifty-four weeks old ($300{\sim}350g$) eight female Sprague-Dawley rats were used. The full-thickness skin biopsy specimens were prepared serial sections and stained with hematoxylin and eosin, Masson's trichrome and Verhoeff-van Gieson. The collagen fiber and the elastic fiber were identified using the image analysis processing system and then calculated the collagen fiber density rate and the elastic fiber density rate in the dermis. It also identified fibroblast and calculated fibroblast density in the dermis. By using a Student's t-test, a decrease in the collagen fiber density rate (t=-4.650, P<0.001) and the elastic fiber density rate (t=-6.494, P<0.001) of dermis can be observed in aged rats as compared with the young rats. A Student's t-test showed a significantly less fibroblast density in the aged rats than the young rats (t=-4.497, P<0.001). There were significantly positive correlation between the fibroblast density and the collagen fiber density rate (r=.69, P<0.001) and the elastic fiber density rate (r=.91, P<0.001). These results indicate that the aging may decrease the collagen fiber density and elastic fiber density due to reduced the proliferative and synthetic activity of fibroblast in the dermis.

• The Journal of Korean Medicine
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• v.38 no.2
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• pp.31-40
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• 2017

Objectives: We investigated the effect of acupuncture on memory function in relation with neurogenesis in old rats. Methods: In this study, a step-down avoidance task for short-term memory and Y-maze task for spatial memory capability were conducted. Western blot analysis for brain-derived neurotorphic factor (BDNF) and tyrosine kinase B (TrkB), and immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU) were performed. Results: Short-term memory and spatial memories were decreased in the old-aged rats. Expressions of BDNF and TrkB in the hippocampus were significantly decreased in the old-aged rats. Neurogenesis in the hippocampal dentate gyrus was also decreased in the old-aged rats. However, acupuncture treatment alleviated impairment of short-term and spatial memories induced by ageing. Acupuncture also increased the expressions of BDNF and TrkB and enhanced neurogenesis in the hippocampus. The present study showed that acupuncture alleviated ageing-induced short-term and spatial memory loss by increasing of BDNF and neurogenesis. Acupuncture at ST41-acupoint showed most potent effect than at ST36-acupoint or non-acupoint. Conclusions: Acupuncture might be used as the effective therapeutic modality to ameliorate the age-related decrease of brain functions.