• Journal of Nutrition and Health
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• v.29 no.8
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• pp.849-860
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• 1996

The supply of different fatty acids during the development period has significant effects. This study examined the effects of dietary $\omega$3 and $\omega$6 fatty acid compositions on phospholipids (PLs) of RBC and rat brain subcellular fractions (synaptosome, microsome, mitochondria), and on learning ability of the 2nd generation rat. Rats were fed experimental diets 3-4 wks prior to the conception. Early in the lactation period, the feeding mothers were exchanged. Diets consisted of 10% fat(by weight), which was either safflower oil('S') poor in $\omega$3 fatty acids or computer-searched mixed oil('M') with P/M/S ratio, 1/1.4/1 and $\omega$6/$\omega$3 ratio, 6.1/1. The 'S' and 'M' rats were subdivided further into SS, SM, MS & MM rats according to their lactation stauts. At 3 (weaning) & 9 wks of age, the percentage of total $\omega$3 fatty acids to their lactation status. At 3 (weaning) & 9 wks of age, the percentage of total $\omega$3 fatty acids and the ratios of $\omega$3/$\omega$6 fatty acids in PLs of RBC and brain subcellular fractions in SM and MM groups fed milk from the mixed oil-fed mothers for 2 wks tended to be higher than those in SS and MS groups respectively. In contrast, the concentrations of $\omega$6 fatty acids, especially 22:5$\omega$6 in all fractions, were significantly lower in the SM & MM groups compared to those of the SS & MS groups respectively. In contrast, the concentration of $\omega$6 fatty acids, especially 22:5$\omega$6 in all fractions, were significantly lower in the SM & MM groups compared to those of the SS & MS groups, The values for the DHA$\omega$3/22:5$\omega$6 ratios after the lactation period were markedly higher in the groups (SM & MM) which were reared by mixed oil(MO) fed mothers. In carring out Y-water maze at 9th wk of age, the SM(4.2$\pm$0.5) & MM (5.3$\pm$0.5) groups made significantly less errors compared to the SS(6.2$\pm$0.6, p<0.05 compared with SM) & MM (7.2$\pm$0.5, p<0.05 compared with MM) groups which were lactated by the safflower oilfed mothers. Therefore, by feeding a balanced fatty acid diet from the lactation period up to 9 wks of age as compared with the groups fed $\omega$3 fatty acid-deficient diet regardless of mother's diet given before parturition. The levels of DHA(synaptosome) and 22:5$\omega$3 (mitochondria) were positively correlated not only with these values in RBC but also with visual discriminating ability. The levels of DHA and 22:5$\omega$3 in RBC can, therfore, reflect visual discriminatng ability in the rat.

• Korean Journal of Veterinary Service
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• v.31 no.3
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• pp.375-384
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• 2008

This study was performed to establish the discrimination of the corpus albicans that were formed after normal parturition and abortion in Holstein, The corpus albicans were obtained from 504 cows and 506 heifers slaughtered in the Agricultural Cooperative's Livestock Products Joint Market of Seoul City from March 2007 to October 2007. The difference in a gross examination (morphology, fibrogenesis, deposit state, color, and size) among the fixtures that exist in their ovaries was investigated. All of the cows had corpus albicans in their ovaries: 423 cows (83.9%) had less than three corpus albicans, 81 (16.1%) had more than four. In the heifers, however, 463 (91.05%) had no corpus albicans, 39 (7.7%) had one, and four (0.8%) had more than two. In the types of corpus albicans 73.2% of the cows had oval-type, 14.4% had circular-shaped, 12.4% had irregular-type. In the aborted heifers, however, oval-type appeared in 88.6% and circular-shaped appeared in 11.4%, and no irregular-type was found. There was no significant difference in the fibrogenesis and size of corpus albicans between cows and aborted heifers. However, slight difference in the color of the corpus albicans was observed between two types. In conclusion, it is not easy to discriminate by gross examination of ovary between cows and aborted heifers in the slaughter house.

• Journal of Aquaculture
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• v.19 no.2
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• pp.125-132
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• 2006

Black rockfish larvae and juveniles were reared for 95 days after parturition (DAP) in order to determine four enzyme activities (trypsin, pepsine-like enzyme, lipase, amylase) during ontogeny. Larvae were fed rotifers Brachionus plicatilis from 1 to 25 DAP, Artemia nauplii from 10 to 78 DAP and then gradually changed to pelleted feed from 30 DAP. Temperature was kept between $13.5{\sim}14.9^{\circ}C$. Trypsin and lipase activities were found in 2 DAP larvae ($7.0{\pm}1.5$ unit and $4.5{\pm}1.4$ unit, $mean{\pm}SD$, respectively). The evolution of both enzymes activities showed a profile marked by drastic increases between postflexion and juvenile stage. There is an increment on specific trypsin activity at 10 DAP, corresponding with the beginning of Artemia feeding. Pepsin-like enzyme activity was found at 11 DAP and increased drastically from 56 DAP, cor-responding with the initiation of juvenile stage. Amylase activity was also found at 11 DAP and maintained at a low level up to 38 DAP followed by a drastic increase from 39 DAP to 50 DAP. Considering our results of both trypsin and pepsin-like enzyme activities, it might be concluded that higher somatic growth of Sebastes inermis could be possible with the initiation juvenile of stage and the early juvenile stage is a suit-able period for feeding an artificial diet for fish.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.9
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• pp.1430-1443
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• 2020

Objective: Twenty-four pregnant Nellore primiparous grazing cows were used to evaluate the effects of energy-protein supplementation and supplementation frequency during pre (105 d before calving) and postpartum (105 d after calving) on performance and metabolic characteristics. Methods: Experimental treatments consisted of a control (no supplementation), daily supplementation (1.5 kg/d of concentrate/animal) and infrequent supplementation (4.5 kg of concentrate/animal every three days). During the pre and postpartum periods, concentrations of blood metabolites and animal performance were evaluated. Ureagenesis and energy metabolism markers were evaluated at prepartum period. Results: Supplementation frequency did not alter (p>0.10) body weight (BW), average daily gain (ADG), and carcass traits during pre and postpartum. The BW (p = 0.079), adjusted BW at day of parturition (p = 0.078), and ADG (p = 0.074) were greater for supplemented cows during the prepartum. The body condition score (BCS; p = 0.251), and carcass traits (p>0.10) were not affected by supplementation during prepartum. On postpartum, supplementation did not affect animal performance and carcass traits (p>0.10). The dry mater intake was not affected (p>0.10) by supplementation and supplementation frequency throughout the experimental period. Daily supplemented animals had greater (p<0.001) glucose levels than animals supplemented every three days. Supplementation and supplementation frequency did not alter (p>0.10) the levels of blood metabolites, neither the abundance of ureagenesis nor energy metabolism markers. Conclusion: In summary, our data show that the reduction of supplementation frequency does not cause negative impacts on performance and metabolic characteristics of primiparous grazing cows during the prepartum.

• Journal of Embryo Transfer
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• v.23 no.1
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• pp.5-11
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• 2008

Serial ultrasonographic examinations were daily performed from 15 days after ovulation until parturition to determine the time of first detection and ultrasonographic appearance of the fetal and extra-fetal structures in pregnant 10 Maltese, 10 Yorkshire Terrier, 15 Shih-tzu, and 10 Miniature Schnauzer bitches, respectively. Gestational age was timed from the day of ovulation (day 0), which was estimated to occur when plasma progesterone concentration was first increased above 4.0ng/ml. The gestational length was $63.4{\sim}63.6$ (range: $61{\sim}65$) days and the geatational length was no statistically significant difference among bitches (p>0.05). The initial detection of the extra-fetal structures were; gestational sac at days $18.9{\sim}19.5\;(17{\sim}22)$, zonary placenta at days $24.6{\sim}25.5\;(23{\sim}28)$, yolk sac membrane at days $24.6{\sim}25.5\;(23{\sim}27)$, yolk sac tubular shape at days $26.1{\sim}26.3\;(24{\sim}28)$, and amniotic membrane at days $26.1{\sim}28.2\;(24{\sim}31)$, respectively. The time of the first detection of the extra-fetal structures were no statistically significant difference among bitches (p>0.05). The initial detection of the fetal structures were; embryo initial detection at days $22.5{\sim}22.9\;(21{\sim}24)$, heartbeat at days $23.2{\sim}23.8\;(21{\sim}25)$, embryo bipolar shape $27.6{\sim}28.9\;(26{\sim}30)$, fetal movement at days $31.9{\sim}32.8\;(27{\sim}34)$, limb buds at days $29.1{\sim}30.7\;(27{\sim}33)$, stomach at days $31.1{\sim}33.1\;(29{\sim}34)$, urinary bladder at days $32.4{\sim}33.2\;(29{\sim}35)$, skeleton at days $34.7{\sim}35.9\;(34{\sim}39)$, and kidney at days $42.1{\sim}44.7\;(41{\sim}48)$, respectively. The the time of the first detection of the fetal structures were no statistically significant difference among bitches (p>0.05). These results indicate the evaluation of the time of first detection and ultrasonographic characteristics of the gestational structures might be useful for pregnancy diagnosis, estimating fetal age, embryonic resorption, fetal monster, abnormal fetal growth and fetal viability, respectively.

• Journal of Veterinary Clinics
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• v.22 no.3
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• pp.253-258
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• 2005

The aim of this study was to estimate the time of ovulation and mating derived by plasma progesterone and estradiol-$17{\beta}$ concentrations. The 11 pregnant Shih-tzu bitches were investigated the plasma progesterone and estradiol-$17{\beta}$ concentrations from proestrus to parturition. Gestation length in the 11 pregnant bitches was $61.9{\pm}1.1\;(mean{\pm}SD)$ days when Day 0 was timed from the day that plasma progesterone concentration was first increased above 4.0 ng/ml. The litter size was $3.8{\pm}0.3$ pups. Plasma progesterone concentrations were increased at the first day of vulvar bleeding and showed at Day 0 with $5.2{\pm}0.3$ ng/ml. It was gradually increased to reach a peak at Day 15 with $42.6{\pm}3.7ng/ml$, thereafter it was gradually decreased to below Day 62. Plasma estradiol-$17{\beta}$ concentrations were increased above 1.0 pg/ml at the first day of vulvar bleeding and showed a peak at Day -2 with $33.5{\pm}8.0$ pg/ml, thereafter it was gradually decreased. When Day 0 was timed from the day that plasma progesterone concentration was first increased above 4.0 ng/ml, plasma estradiol-$17{\beta}$ concentration reached a peak at Day -2. In conclusion, these results indicated that ovulation was estimated to occur the day when plasma progesterone concentration was first increased above 4.0 ng/ml after the first day of bleeding. It was estimated that mating time was the day when plasma progesterone concentration was between $3.0{\~}8.0$ ng/ml.

• Journal of Veterinary Clinics
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• v.14 no.2
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• pp.301-307
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• 1997

These studies were performed to provide some basic informations for developing an automatic system in dairy farming cattle in order that the farmers may easily and automatically detect the maintenance of pregnancy and the fact of abortion of the pregnant cows and also to find out the diseased animals with fever. As a method of automatical detection of the maintenance of pregnancy or the fact of abortion, weighing the pregnant cows was conducted from one month-pregnancy to the term using a digital balance. From the first to the 3rd month of pregnancy the body weight of dairy cows was slowly increased (less than 2% per month), then, relatively high increase (3.4% -4.3% per month) from the fourth to the seventh month followed by decrease (3.3%) in the 8th month and very low increase (0.8-0.9%) from the 9th month to the term were shown, resulting in increase of 128.8 kg (25.05%) of body weight to be compared with the first weight. More than 107, increase of body weight to be compared with the first month-weight was denoted from the 61th month of pregnancy and more than 20% increase from the 7th or the 8th month of pregnancy as wells consequently it was presumed that detection of the maintenance of pregnancy is possible from the 4th or the 5th month of pregnancy. It was possible to diagnose a cow aborted at the 6th month by continual weighing the cow from the 1st month of pregnancy. The calved cows showed considerably higher decrease of body weight even in the third week after parturition (p<0.01)to be compared with the body weight near to the term (81.8-102.0 kg, 14-16% decrease). During the same period of 8months, the pregnant cows gained 127.4 kg (24.78% increase), whereas the non-pregnant cows gained 33.0 kg (0.71% increase) to be compared with the first weight showing considerably higher increase of body weight gain in the pregnant cows than the non-pregnant cows (p<0.01). The statistics of body temperatures of dairy cattle were collected from three clinics including the Teaching Hospital of Chonbuk University and the diseases were classified simply by the major symptoms manifested, denoting the highest temperature in respiratory disease ($39.8{\circ}C$) and the lowest in alimentary disease ($39.6{\circ} C$). These informations of body temperatures were expected to be of value for early and automatical detection of the diseased animals with fever when automatic machinery would be established. The results of periodic weighing the body weight of pregnant cows while milking were also expected to be of great use for the farmers to detect the maintenance of pregnancy and the fact of abortion when the automatic system is established in the near future.

• Reproductive and Developmental Biology
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• v.37 no.4
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• pp.263-267
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• 2013

Pig producers have been shown keen interest of the number of spermatozoa in a semen dose since pig artificial insemination introduce. However, determining the minimal number of spermatozoa need per AI without detrimental effect on overall reproductive performances is not an easy question to answer. To increase the efficiency of semen utilization in pig AI, optimum number of spermatozoa per dose needed to determine. The objective of this study was to determine the reproductive performance and factors that affect on-farm application of low-dose semen insemination in sows. Data were collected from Darby Genetics AI studs from 4th of June to 7th of July, 2012 (n=401). The numbers of parturition were 84, 234 and 83 in sows inseminated with doses of $1.5{\times}10^9$, $2.0{\times}10^9$ and $2.5{\times}10^9$ spermatozoa in 100ml extender, respectively. There were no significant differences on reproductive performances such as gestation period, total born, total born alive, stillbirth and mummy in sows inseminated with different semen doses. The average number of born alive was 10.5, 11.0 and 10.4 from sows inseminated with $1.5{\times}10^9$, $2.0{\times}10^9$ and $2.5{\times}10^9$ sperms, respectively. Also, number of spermatozoa per dose did not affect litter size (p>0.10). There were no significant differences of maternal genetic line difference on gestation period, total number born, number born alive, born dead and mummy. The estimated correlation coefficients of the different semen doses with total number born, number born alive, born dead and mummy were r=-0.00, -0.01, 0.02 and 0.02, respectively. Taken together, the result of this study suggested that when semen was appropriately inseminated after induced ovulation, insemination with low-dose ($1.5{\sim}2.0{\times}10^9$) semen dose not adversely affect sow's fertility.

• Journal of The Korean Society of Grassland and Forage Science
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• v.44 no.2
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• pp.83-91
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• 2024

We compared the reproductive performance, non-productive performance, and milk composition performance of two groups of lactating cows that were either rice straw or tall fescue. We also compared and analyzed these parameters after the first parturition during the breeding period. The calving interval, artificial insemination, and duration from calving to conception were longer in the rice straw group than in the tall fescue group. The 305-day and peak milk yields were significantly higher in the tall fescue group than in the rice straw group (p<0.01). Milk fat content was significantly higher in the tall fescue group than in the rice straw group (p<0.001), but milk protein content was the same between the feeding groups. The difference in raw milk sales income between rice straw and tall fescue groups tended to increase as the number of lactations increased, but there was no effect of reduced feed costs. Analysis of the milk production and raw milk sales income of the surveyed farms revealed that the average milk production per head was significantly (p<0.05) higher in the tall fescue group than in the rice straw group, and raw milk sales income showed a similar tendency. Overall, these results provide important details to be considered when selecting feed as a way to reduce milk production and heifer raising costs. However, there remains a need for future follow-up studies exploring the relationship between feed choice and the management of heifers.

• Korean Journal of Agricultural Science
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• v.19 no.1
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• pp.51-64
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• 1992

This experiment was carried out to elucidate the effects of the thyroid function on lactation in female rats. One hundred and five female rats, whose body weight was approximately 250g with normal parturition, were divided into 3.5 THY, 35 PTU, and 35 CON. The $30{\mu}g$ L-thyroxine per rat was administered subcutaneously for the THY group with 3-days intervals arid 0.03% propylthiouracil solution was drunk for the PTU group. After the treatments body weight, thyroid weights and prolactin levels in serum, and histological changes in thyroid and mammary gland were investigated for 3 weeks with 3-days interval. The results obtained were as follows 1. The body weights of PTU group were lower than those of CON arid THY groups. The changes in body weights were significant between 3 and 6 days and between 15 and 18 days. 2. Differences in thyroid weight among the groups were significant after 9 days, The thyroid weights of PTU group were much higher than those of CON and THY group. 3. The follicular epithelia of PTU group after 6 days showed cuboidal phenomena which were accompanied by hypertrophy and hyperplasia, and this phenomena continued until post weaning period. Those of THY group after 9 days showed a squamous degeneration together with pyknosis. 4. The prolactin concentrations of THY group were higher than the other groups after 12 days. and those of CON group were higher than the others after 18 days. However, those of PTU group were lower all through the period. 5. The secretary epithelial cells of THY and CON groups became cuboidal after 12 days, but after 15 days the differentiation of mammary tissue was progressing in THY group faster than CON group. The degeneration of mammary tissue were observed in PTU group as time lapses, so after 15 days the exfoliation of secretory epithelium and atrophy of alveolus were recognized. 6. The body weights of offspring for all experimental groups were increasing as time lapses, but the values for PTU group were markedly lower than the others.