• Journal of Marine Bioscience and Biotechnology
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• v.5 no.4
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• pp.26-32
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• 2011

Green fluorescent protein (GFP), a very important biological agent that involves shifting the color of bioluminescence from blue to green in luminous coelenterates and to increase the quantum yield of light emission. GFP discovered in medusa, Aequorea victoria is a key factor of various biotechnological and cell biological applications. Beside these applications, GFP of A. victoria is generally stable, which does not require co-factors for activity and can be functionally expressed in different bacterial species. This property of GFPs from A. victoria permits them to be a unique tool to monitor gene expression and protein localization in different organisms. The present review brings out the past milestones and future perspectives on GFPs, with an elaborative reviewing on its applications.

• Proceedings of the Korean Society of Life Science Conference
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• 2003.05a
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• pp.38-47
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• 2003

처음으로 real time으로 유전자 발현을 visualization할 수 있는 marker를 이용할 수 있게 되었다. 이 maker가 바로 green fluorescent protein(GFP; 녹색형광단백질)이다. GFP는1962년 Shimomura 등에 의하여 해파리인 Aequorea victoria에 존재함이 밝혀졌다(1). 그러나 30년이 지난 1992년이 되어서야 Plasher등에 의하여 GFP의 cDNA가 클로닝 되었고(2) 이후 지금까지 약 10년 동안 GFP는 생명과학분야에서 가장 각광받는 유용한 단백질 중의 하나가 되어 매우 다양한 연구에 응용이 되고 있다. 이렇게 GFP가 생명과학분야에서각광을 받게 된 이유로는 GFP가 모든 외래의 세포에서 형광을 발할 수 있는 활성을 가진 형태로 발현되기 때문이다(3). Chalfie 등은 처음으로 GFP를 대장균과 Caenorhabditis elegans에서 발현시켜 유전자의 발현을 모니터함으로써 GFP를 원핵 및 진핵세포 모두에서 사용할 수 있다는 것을 보고하였다(3), 이러한 발견을 통하여 GFP는 살아있는 세포, 조직 및 생물체에 해를 주지 않고 (non-invasive) 유전자의 발현을 측정하는 marker로서 사용할 수 있게 되어 cell biology, developmental biology, neurobiology 및 cytology 등의 연구분야에 널리 이용되고 있다.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.460-466
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• 2010

A mutant of green fluorescent protein ($GFPmut3^*$) from the jellyfish Aequorea victoria was cyclized in vitro and in vivo by the use of a naturally split intein from the dnaE gene of Synechocystis species PCC6803 (Ssp). Cyclization of $GFPmut3^*$ was confirmed by amino acid sequencing and resulted in an increased electrophoretic mobility compared with the linear $GFPmut3^*$. The circular $GFPmut3^*$ was $5^{\circ}C$ more thermostable than the linear form and significantly more resistant to proteolysis of exopeptidase. The circular $GFPmut3^*$ also displayed increased relative fluorescence intensity. In addition, chemical stability of $GFPmut3^*$ against GdnHCl revealed more stability of the circular form compared with the linear form.

• International Journal of Industrial Entomology and Biomaterials
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• v.1 no.2
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• pp.149-153
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• 2000

We have constructed a recombinant baculovirus, Autographa californica nuclear polyhedrosis virus (AcNPV), containing green fluorescent protein (GFP) gene from the jellyfish, Aequorea victoria, and transferred it into the domestic silkworm Bombyx mori larvae for the production of visible transgenic silkworm of living organism. When one day-old fifth instar female larvae were injected with the recombinant AcNPV of 1x10$^{5}$ plaque forming units, the bright glow of GFP was detected in the recombinant AcNPV-infected larvae and in the newly hatched larvae of the next generation. Our findings demonstrate that the viral replication was detected in the silkworm treated with the recombinant ACNPV and the gfp gene was expressed under the transcriptional control of the polyhedrin gene promoter, Furthermore, the gfp gene was transmitted to the next generation, suggesting that this system can be applied for the development of transgenic silkworms.