Protein Cyclization Enhanced Thermostability and Exopeptidase-Resistance of Green Fluorescent Protein
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Zhao, Zhonglin
(Biotechnology Research Institute, Chinese Academy of Agricultural Sciences)
Ma, Xin (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences) Li, Liang (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences) Zhang, Wei (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences) Ping, Shuzhen (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences) Xu, Ming-Qun (New England Biolabs, Inc.) Lin, Min (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences) |
1 | Cormack, B. P., R. H. Valdivia, and S. Falkow. 1996. FACSoptimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38. DOI ScienceOn |
2 | Evans, T. C. Jr., D. Martin, R. Kolly, D. Panne, L. Sun, I. Ghosh, et al. 2000. Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803. J. Biol. Chem. 275: 9091-9094. DOI |
3 | Grantcharova, V. P., D. S. Riddle, and D. Baker. 2000. Longrange order in the src SH3 folding transition state. Proc. Natl. Acad. Sci. U.S.A. 97: 7084-7089. DOI ScienceOn |
4 | Iwai, H., A. Lingel, and A. Pluckthun. 2001. Cyclic green fluorescent protein produced in vivo using an artificially split PI-PfuI intein from Pyrococcus furiosus. J. Biol. Chem. 276: 16548-16554. DOI |
5 | Iwai, H. and A. Pluckthun. 1999. Circular beta-lactamase: Stability enhancement by cyclizing the backbone. FEBS Lett. 459: 166-172. DOI ScienceOn |
6 | Jeffries, C. M., S. C. Graham, P. H. Stokes, C. A. Collyer, J. M. Guss, and J. M. Matthews. 2006. Stabilization of a binary protein complex by intein-mediated cyclization. Protein Sci. 15: 2612-2618. DOI ScienceOn |
7 | Jermutus, L., M. Tessier, L. Pasamontes, A. P. van Loon, and M. Lehmann. 2001. Structure-based chimeric enzymes as an alternative to directed enzyme evolution: Phytase as a test case. J. Biotechnol. 85: 15-24. DOI ScienceOn |
8 | Otzen, D. E. and A. R. Fersht. 1998. Folding of circular and permuted chymotrypsin inhibitor 2: Retention of the folding nucleus. Biochemistry 37: 8139-8146. DOI ScienceOn |
9 | Thornton, J. M. and B. L. Sibanda. 1983. Amino and carboxyterminal regions in globular proteins. J. Mol. Biol. 167: 443-460. DOI |
10 | Trabi, M. and D. J. Craik. 2002. Circular proteins - no end in sight. Trends Biochem. Sci. 27: 132-138. DOI |
11 | Xu, M. Q. and T. C. Jr. Evans. 2001. Intein-mediated ligation and cyclization of expressed proteins. Methods 24: 257-277. DOI ScienceOn |
12 | Hofmann, A., H. Iwai, S. Hess, A. Pluckthun, and A. Wlodawer. 2002. Structure of cyclized green fluorescent protein. Acta Crystallogr. D Biol. Crystallogr. 58: 1400-1406. DOI ScienceOn |
13 | Wu, H., Z. Hu, and X. Q. Liu. 1998. Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. Proc. Natl. Acad. Sci. U.S.A. 95: 9226-9231. DOI ScienceOn |
14 | Andersen, J. B., C. Sternberg, L. K. Poulsen, S. P. Bjorn, M. Givskov, and S. Molin. 1998. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64: 2240-2246. |
15 | Evans, T. C. Jr., J. Benner, and M. Q. Xu. 1999. The cyclization and polymerization of bacterially expressed proteins using modified self-splicing inteins. J. Biol. Chem. 274: 18359-18363. DOI |
16 | Grantcharova, V. P. and D. Baker. 2001. Circularization changes the folding transition state of the src SH3 domain. J. Mol. Biol. 306: 555-563. DOI ScienceOn |
17 | Rodriguez, E., Z. A. Wood, P. A. Karplus, and X. G. Lei. 2000. Site-directed mutagenesis improves catalytic efficiency and thermostability of Escherichia coli pH 2.5 acid phosphatase/phytase expressed in Pichia pastoris. Arch. Biochem. Biophys. 382: 105-112. DOI ScienceOn |
18 | Scott, C. P., E. Abel-Santos, M. Wall, D. C. Wahnon, and S. J. Benkovic. 1999. Production of cyclic peptides and proteins in vivo. Proc. Natl. Acad. Sci. U.S.A. 96: 13638-13643. DOI ScienceOn |
19 | Hruby, V. J. 1982. Conformational restrictions of biologically active peptides via amino acid side chain groups. Life Sci. 31: 189-199. DOI ScienceOn |
20 | Williams, N. K., E. Liepinsh, S. J. Watt, P. Prosselkov, J. M. Matthews, P. Attard, J. L. Beck, N. E. Dixon, and G. Otting. 2005. Stabilization of native protein fold by intein-mediated covalent cyclization. J. Mol. Biol. 346: 1095-1108. DOI ScienceOn |
21 | Zhao, Z., W. Lu, B. Dun, D. Jin, S. Ping, W. Zhang, M. Chen, M. Q. Xu, and M. Lin. 2008. Purification of green fluorescent protein using a two-intein system. Appl. Microbiol. Biotechnol. 77: 1175-1180. DOI ScienceOn |
22 | Lehmann, M., C. Loch, A. Middendorf, D. Studer, S. F. Lassen, L. Pasamontes, A. P. van Loon, and M. Wyss. 2002. The consensus concept for thermostability engineering of proteins: Further proof of concept. Protein Eng. 15: 403-411. DOI |
23 | Kanno, A., Y. Yamanaka, H. Hirano, Y. Umezawa, and T. Ozawa. 2007. Cyclic luciferase for real-time sensing of caspase 3 activities in living mammals. Angew. Chem. Int. Ed. Engl. 46: 7595-7599. DOI ScienceOn |
24 | Williams, N. K., P. Prosselkov, E. Liepinsh, I. Line, A. Sharipo, D. R. Littler, P. M. Curmi, G. Otting, and N. E. Dixon. 2002. In vivo protein cyclization promoted by a circularly permuted Synechocystis sp. PCC6803 DnaB mini-intein. J. Biol. Chem. 277: 7790-7798. DOI |
25 | Lehmann, M., L. Pasamontes, S. F. Lassen, and M. Wyss. 2000. The consensus concept for thermostability engineering of proteins. Biochim. Biophys. Acta 1543: 408-415. DOI ScienceOn |
26 | Lehmann, M., R. Lopez-Ulibarri, C. Loch, C. Viarouge, M. Wyss, and A. P. van Loon. 2000. Exchanging the active site between phytases for altering the functional properties of the enzyme. Protein Sci. 9: 1866-1872. DOI ScienceOn |
27 | Siebold, C. and B. Erni. 2002. Intein-mediated cyclization of a soluble and a membrane protein in vivo: Function and stability. Biophys. Chem. 96: 163-171. DOI ScienceOn |
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