International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Construction of the Silkworm, Bombyx mori, with a Green Fluorescence by Autographa californica Nuclear Polyhedrosis Virus

  • Jin, Byung-Rae (Department of Sericulture and Entomology, The National Institute of Agricultural Science and Technology) ;
  • Yun, Eun-Young (Department of Sericulture and Entomology, The National Institute of Agricultural Science and Technology) ;
  • Kang, Seok-Woo (Department of Sericulture and Entomology, The National Institute of Agricultural Science and Technology) ;
  • Yoon, Hyung-Joo (Department of Sericulture and Entomology, The National Institute of Agricultural Science and Technology) ;
  • Kim, Keun-Young (Department of Sericulture and Entomology, The National Institute of Agricultural Science and Technology) ;
  • Kim, Ho-Rak (Department of Sericulture and Entomology, The National Institute of Agricultural Science and Technology) ;
  • Je, Yeon-Ho (College of Agriculture and Life Sciences, Seoul National University) ;
  • Kang, Seok-Kwon (College of Agriculture and Life Sciences, Seoul National University)
  • Published : 2000.12.01
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Abstract

We have constructed a recombinant baculovirus, Autographa californica nuclear polyhedrosis virus (AcNPV), containing green fluorescent protein (GFP) gene from the jellyfish, Aequorea victoria, and transferred it into the domestic silkworm Bombyx mori larvae for the production of visible transgenic silkworm of living organism. When one day-old fifth instar female larvae were injected with the recombinant AcNPV of 1x10$^{5}$ plaque forming units, the bright glow of GFP was detected in the recombinant AcNPV-infected larvae and in the newly hatched larvae of the next generation. Our findings demonstrate that the viral replication was detected in the silkworm treated with the recombinant ACNPV and the gfp gene was expressed under the transcriptional control of the polyhedrin gene promoter, Furthermore, the gfp gene was transmitted to the next generation, suggesting that this system can be applied for the development of transgenic silkworms.

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