• Biomolecules & Therapeutics
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• v.25 no.5
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• pp.511-518
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• 2017

Ultraviolet (UV) irradiation is a relevant environment factor to induce cellular senescence and photoaging. Both autophagy- and silent information regulator T1 (SIRT1)-dependent pathways are critical cellular processes of not only maintaining normal cellular functions, but also protecting cellular senescence in skin exposed to UV irradiation. In the present studies, we investigated whether modulation of autophagy induction using a novel synthetic SIRT1 activator, heptasodium hexacarboxymethyl dipeptide-12 (named as Aquatide), suppresses the UVB irradiation-induced skin aging. Treatment with Aquatide directly activates SIRT1 and stimulates autophagy induction in cultured human dermal fibroblasts. Next, we found that Aquatide-mediated activation of SIRT1 increases autophagy induction via deacetylation of forkhead box class O (FOXO) 1. Finally, UVB irradiation-induced cellular senescence measured by $SA-{\beta}-gal$ staining was significantly decreased in cells treated with Aquatide in parallel to occurring SIRT1 activation-dependent autophagy. Together, Aquatide modulates autophagy through SIRT1 activation, contributing to suppression of skin aging caused by UV irradiation.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.11 no.1
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• pp.85-91
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• 2001

Background : It goes without saying that cumulative trauma disorders is spreading to various types of occupation in many advanced countries including America, and it forms considerable rate of total occupational disorders already. and as the result of it, the seriousness of worker health problem and economic loss owing to production loss, recuperation expense, etc. are on the increasing trend through whole society. In case of Korea, the related measures as well as accurate fact-finding survey data on cumulative trauma disorders aren't prepared in detail, so it implies forward problem would be serious. Purpose : The purpose of this study is to draw the risk factor of cumulative trauma disorders in production factory, to forecast the rate of occurrence of cumulative trauma disorders on the basis of subjective symptoms, and to present forward realistic and effective prevention measures by showing the risk of cumulative trauma disorders, objecting the production factory of a domestic riding automobile assembly shop, by estimating in the aspect of human-engineering through the analysis of risk factor being a cause of cumulative trauma disorders. Methods : For this study, I investigated work type and on-duty hours, breathing time, and subjective symptoms of cumulative trauma disorders through questionnaire, objecting the workers in press. car body, coating, and outfit factory. Results : As a result of research, 81.2% of workers were in the physical burden due to present working, and the highest prevalence by part of body is an waist. The higher a rule score, The higher the number of workers complaind for working intensity, and the higher age work duration, tool used time, the higher prevalence of subjective symptoms by part of body. The number of workers complaind subjective symptom for treatment is the highest in drugstore.

• The Journal of Internal Korean Medicine
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• v.39 no.3
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• pp.372-388
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• 2018

Objective: This study evaluates the effectiveness and safety of moxibustion for benign prostatic hyperplasia (BPH). Methods: Using the keywords "benign prostatic hyperplasia", "benign prostatic hypertrophy", "benign prostatic enlargement", "prostatic hyperplasia", and "moxibustion", we searched papers in numerous databases, including National Discovery for Science Leaders (NDSL), Korean Traditional Knowledge Portal (KTKP), Oriental Medicine Advanced Searching Integrated System (OASIS), Research Information Sharing Service (RISS), PubMed, Embase, and CENTRAL. The search range included randomized controlled trials (RCTs). Papers not matched with inclusion criteria were excluded. The methodological quality of each RCT was assessed using the Cochrane risk-of-bias tool. Where appropriate, meta-analyses were performed. Results: Initially, 77 studies were found. Of these, 11 duplicate studies were removed and 27 were excluded following title and abstract screening. After the remaining 39 papers were scanned, 13 RCTs were selected and analyzed. Among these 13 RCTs, five compared moxibustion therapy and oral medication, seven compared moxibustion plus acupuncture therapy and oral medication, and one compared moxibustion plus acupuncture therapy and sham-moxibustion. The meta-analysis showed positive results for the use of moxibustion therapy in terms of International Prostate Symptom Score (IPSS), Quality Of Life (QOL), Maximum Flow Rate (Qmax), Prostate Volume (PV), and the efficacy rate. The meta-analysis showed positive results for the use of moxibustion plus acupuncture therapy in terms of IPSS, QOL, and the efficacy rate. Conclusions: This meta-analysis of clinical trials suggests that moxibustion is effective intreating BPH patients. The results of this study could be applied to clinical treatment of BPH. However, additional large-scale clinical researches should be conducted.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.540-544
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• 2010

The nursery plant quality and flower bud induction of new strawberry cultivars, 'Maehyang' and 'Seolhyang' in forcing culture were evaluated in the highland and lowland region. In order to produce daughter plants, the new cultivars were grown in the open field located at both highland (Daekwallyung, above 800 m sea level) and lowland (Gangneung, above 20 m sea level) region, respectively. The average air temperature at highland during nursery plant propagation period was $5.3^{\circ}C$ lower than those at lowland. The number of daughter plants produced at lowland was 2 times as high as those of highland, presumably due to the higher air temperature. Anthracnose incidence rates of runner plants produced at lowland were 4 to 7% as high as those in highland. Mean temperature in the highland ($23.5^{\circ}C$) during flower bud differentiation treatment was $5.1^{\circ}C$ lower than that in the lowland ($28.6^{\circ}C$). Seedlings produced in highland showed higher C/N ratio and lower T/R ratio compared to those produced in lowland. The average flower bud formation date of the daughter plants grown in highland were advanced by 30 days compared to the date in lowland. Accordingly, highland was supposed to be appropriate region for raising seedlings in forcing culture of the new strawberry cultivars of 'Maehyang' and 'Seolhyang'.

• Journal of Chest Surgery
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• v.39 no.8 s.265
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• pp.633-636
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• 2006

The partial endocardial cushion defect including ostium primum atrial septal defect and anterior mitral leaflet cleft, presents less significant clinical symptoms than complete endocardial cushion defect. But, as mitral insufficiency develops, cardiomegaly, congestive heart failure, pulmonary arterial hypypertension appear. So, partial endocardial cushion defect has poor prognosis and is rarely seen in elderly patients. A 67 years old woman admitted at our hospital for operative treatment with partial endocardial cushion defect. She had increased pulmonary pressure of 45/22 mmHg, mean 32 mmHg. She had repair of ostium primum defect with patch, and the mitral valve was treated with valve replacement. Because advanced atrioventricular block developed postoperatively, she received permanent pacemaker.

• Microbiology and Biotechnology Letters
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• v.37 no.1
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• pp.17-23
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• 2009

The gene encoding Thermus thermophilus HJ6 DNA polymerase (Tod) was cloned and sequenced. The open reading frame (ORF) of the Tod gene was composed of 2,505 nucleotides and encoded a protein (843 amino acids) with a predicted molecular weight of 93,795 Da. The deduced amino acid sequence of Tod showed 98% and 86% identities to the Thermus thermophilus HB8 DNA pol and Thermus aquaticus DNA pol, respectively, The Tod gene was expressed under the control of the bacteriophage $\lambda$ promoters PR and PL on the expression vector pJLA503 in Escherichia coli strain BL21 (DE3) codon plus. The expressed enzyme was purified by heat treatment, $HiTrap^{TM}$ Q column, and $HiPrep^{TM}$ Sephacryl S-200 HR 26/60 column chromatographies. The optimal temperature and pH for DNA polymerase activity were found to be $75{\sim}80^{\circ}C$ and 9.0, respectively. The optimal concentrations of $Mg^{2+}$ and $Mn^{2+}$ were 2.5 mM and 1 mM, respectively. The enzyme activity was activated by divalent cations, and was inhibited by monovalent cations. The result of the PCR experiment with Tod DNA polymerase indicates that this enzyme might be useful in DNA amplification and PCR-based applications.

• Microbiology and Biotechnology Letters
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• v.47 no.4
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• pp.630-638
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• 2019

Nitrous oxide (N₂O) is a greenhouse gas with a global warming potential 310 times higher than that of carbon dioxide. In this study, an N₂O-reducing consortium was obtained by enrichment culture using advanced treatment sludge as the inoculum. The dominant bacteria in the consortium were Sulfurovum (17.95%), Geobacter (14.63%), Rectinema (11.45%), and Chlorobium (8.24%). The consortium displayed optimal N₂O reducing activity when acetate was supplied as the carbon source at a carbon/nitrogen ratio (mol·mol^-1) of 6.3. The N₂O reduction rate increased with increasing N₂O concentration at less than 3,000 ppm. Kinetic analysis revealed that the maximum N₂O reduction rate of the consortium was 163.9 ㎍-N·g-VSS^-1·h^-1. Genes present in the consortium included nosZ (reduction of nitrous oxide to N₂), narG (reduction of nitrate to nitrite), nirK (reduction of nitrite to nitric oxide), and norB (reduction of nitric oxide to nitrous oxide). These results indicate that the N₂O-reducing consortium is a promising bioresource that can be used in denitrification and N₂O mitigation.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.4 no.1
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• pp.77-85
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• 2006

A demonstration facility for an advanced spent fuel conditioning process (ACP) is under construction at KAERI. In this hotcell facility, the rear door is frequently used since all process equipment and materials are taken in and out only through the rear door. Therefore , both the structural safety and stability of the door are essentially required for the safety of ACP facility. In this paper, the finite element analysis has been performed to investigate the structural safety under the impact condition between the rear door and the door frame. Also the possibility of the rear door being tumbled over by the impact force or the inertia force under a sudden stop conditon has been evaluated. The analysis results demonstrate that the structural safety and stability of the rear door are sufficiently assured for both the impact and the accidential stop conditions.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.4 no.1
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• pp.17-24
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• 2006

A demonstration facility for advanced spent fuel conditioning process (ACP) is under construction in KAERI. In this hot cell facility, all process equipments and materials are taken in and out only through the rear door. The working table in front of the process rear door is specially designed to be openable for the efficient use of the space. This paper presents the structural safety analysis of the openable working table, for the normal operational load condition and accidential drop condition of heavy object. Both cases are investigated through static and dynamic finite element analyses. The analysis results show that structural safety of the working table is sufficiently assured and the working table is not collapsed even when an object of 500 kg is dropped from the height of 50 cm.

• Journal of Dairy Science and Biotechnology
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• v.25 no.1
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• pp.15-20
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• 2007

Effects of gamma irradiation on chemical, microbiological, and immunological changes of Queso Blanco cheese were investigated. Although Queso Blanco cheese was made by heat pasteurization at 85$^{\circ}$C and addition of acid without lactic starter culture, total bacterial counts and lactic acid bacterial counts of control cheese were 7.65${\pm}$0.04 and 7.64${\pm}$0.02 log CFU/mL, respectively. It was thought that this microbial growth was due to the incomplete inactivation of raw milk by the heat treatment, resulting into growth during the pressing and the drying process. It demonstrated the possibility that if heat- and acid-resistant hazard microbes are present in raw milk, they can grow during the processes. Lactic acid bacterial counts of the irradiated cheese were 5.45${\pm}$0.02 log CFU/mL at 1kGy, 2.12${\pm}$0.12 log CFU/mL at 2kGy, and not detected at 3kGy or higher doses. The reduction of antigenicity by gamma irradiation was not found. It might be caused by the fact that most whey proteins of milk, a major antigen in milk, were already denaturated by heat process and removed during the draining.