• 방사선산업학회지
• /
• 제4권4호
• /
• pp.297-306
• /
• 2010

Due to the increasing incidence of diabetes, obesity and hypertensive, stevia has been placed great attentions as the sweetener to substitute sucrose in the world. Stevia was introduced to Korea in 1970's, but it has not been an attractive crop in that time. However, recently it has more attention for the natural food sweet additives. Because stevia have many problems for cultivation especially cultivar, seed germination, fertility, uniformity and glycoside quality, the sport mutation was attempted to in vitro plants for the improvement of some characteristics. The young in vitro plants was nursed on MS medium supplemented with $1mg\;l^{-1}\;GA_3$. Shoots of 10 cm height were irradiated with 0~200 Gy of gamma ray and the every node was separated and inoculated on MS basic medium. The lethality, number and length of shoot, numbers of node and branch were investigated for the evaluation of radiosensitivity. The optimum dose of gamma ray seemed to be around 80 Gy for the sport mutation induction in stevia. The lower node was more sensitive than higher node to radiation.

• 한국학교수학회논문집
• /
• 제25권4호
• /
• pp.333-352
• /
• 2022

본고는 초등수학을 기반으로 첨단기술및 ICT교구를활용한 초등융합교육자료의 개발에 대한 사례 연구이다. 본 연구에서는 초등현장 교육전문가 6인이 준비-개발-개선의 PDI 모형에 따라 융합교육 자료를 개발하였고, 개발된 자료는 수학, 과학, 미술, 공학 과목의 교과교육전문가 4인에 의해서 수정·보완하였다. 개발된 융합교육 자료는 초등 3~4학년군에서 활용 가능한 『그래프! 과거, 현재, 미래를 이어주는 다리』 의 1종이고, 5~6학년군은『알쏭달쏭, 같은 듯 다른 너!』 , 『함께 만드는 가상현실 입체 공간』의 2종이며, 초등 3~4학년군과 5~6학년군에서 학년군별로 재구성해 사용 가능한 『그리고 만드는 재미있는 도형 나라』 , 『거북선 지붕을 빈틈없이 덮어라!』 의 2종이었다. 본 개발 연구의 결과를 기반으로 한 제언은 다음과 같다. 초등교육 현장에서 원활한 융합교육을 수행할 수 있도록 다양한 융합교육 자료가 개발 공유되어야 하며, 나아가 앞으로 융합교육의 확산과 정착을 위해서는 현장 초등 교사나 예비 초등 교사들의 융합교육 역량의 함양 및 신장이 절대적인 선결 조건일 것이다.

• International Journal of Advanced Culture Technology
• /
• 제11권1호
• /
• pp.221-231
• /
• 2023

Nowadays, more and more countries are paying attention to basic teaching, so it can be found that they are carrying out a new round of basic teaching reform. The fundamental purpose is to adapt to the economic, cultural and technological development of the 21st century, so that their nationals will not be eliminated in the competition of the world environment, the spiritual civilization and material life of the nationals can be further improved, and they can have an advantage in the competition and catch up with the advanced level of the world. The cultivation of preschoolers' mental health determines the future development of a person. Through the long-term practice of art teaching at the Longyuan Branch of the District Third Kindergarten of Guangxi, This paper summarizes some effective teaching methods to cultivate children's connection ability and sense of security through aesthetic education.Firstly, preschoolers' contact ability was studied, and the aspects such as the definition of contact ability and the reasons for the formation of contact ability were explored. Secondly, the development of preschoolers' sense of security, what to avoid in order to develop a good sense of security and the effects of security on children were investigated. Finally, several specific teaching cases were written based on these theories. Through these cases, aesthetic education was utilized to promote preschoolers' contact ability and develop a sense of security. The study mainly draws the following conclusions that if children want to have a successful career, a happy family in the future, it is crucial to have the ability of connect and security in childhood. Aesthetic education can promote the formation of children's ability of connection and security.Finally, I write specific teaching cases based on the above theories and practice, through which these cases use aesthetic education to promote the formation of children's contact ability and sense of security.

• 한국자원식물학회지
• /
• 제27권3호
• /
• pp.242-248
• /
• 2014

This study was conducted to establish an efficient transformation system by using somatic embryogenesis in an important Korean native conifer, Korean fir (Abies koreana). Embryogenic masses were induced from mature zygotic embryos of the Korean fir on Schenk and Hildebrandt medium, which was supplemented with thidiazuron. For genetic transformation, the embryogenic masses were co-cultivated with a disarmed Agrobacterium tumefaciens strain C58/pMP90 containing the plasmid vector pBIV10 or LBA4404 containing the plasmid vector MP90. Both vectors contain the kanamycin resistance and beta-glucuronidase (GUS) reporter genes. A total of 48 lines of embryogenic masses were selected on mLV medium containing $50{\mu}g/mL$ of kanamycin after 4 weeks of culture, following 3 days of co-cultivation with A. tumefaciens strain C58/pMP90 carrying pBIV10 (none of the lines was cultivated with strain LBA4404 carrying MP90). Quantitative real-time PCR was performed, and high levels of GUS transcripts were observed in the 48 putative transgenic lines; however, the control (non-transgenic line) showed negative results. Results of histochemical staining showed that the expression of the GUS reporter gene was observed in somatic embryos that developed from the embryogenic masses of all 48 lines. Stably transformed cultures were successfully produced by co-cultivation with A. tumefaciens strain C58/pMP90 carrying pBIV10 in Korean fir. Here, we have reported an Agrobacterium-mediated gene transfer protocol via somatic embryogenesis that may be helpful in developing breeding and conservation strategies for the Korean fir.

• 생명과학회지
• /
• 제30권9호
• /
• pp.798-803
• /
• 2020

1960년대 후반 인디카 품종과 자포니카 품종의 교잡에 의하여 새로운 통일형 벼 품종을 육성했고 그 재배기술을 확립했으며, 현재까지 이 재배기술을 이용하여 소비자들의 기호에 맞게 그리고 극한환경에 대응할 수 있는 다양한 품종들이 만들어졌다. 서로 다른 작물끼리 교배하여 얻은 F₁을 가지고 반복적인 세대 진전을 통하여 도입을 원하는 우량한 유전자원의 형질이 더 이상 분리되지 않을 때 교배시킨 형질이 고정되었다고 볼 수 있으며 비로소 신품종이 될 수 있다. 본 연구에서는 가뭄에 저항성을 띠는 HV23 계통을 모품종인 Ilmi와 교배한 집단과 벼멸구에 내성을 갖는 Drimi2ho와 교배한 집단의 F₄, F₅, 그리고 F₆의 농업형질을 조사하여 고정 여부를 확인했다. HV23와 Drimi2ho/HV23 계통의 F₅, F₆를 비교하였을 때 그리고 Ilmi/HV23 계통의 F₄, F₅를 비교하였을 때 모두 유의하지 않은 정도의 형질 변이가 나타났기 때문에 농업형질이 고정되었다고 판단할 수 있었다. 일반적인 계통 육종법은 품종개량에 10년 이상의 시간이 드는 반면 본 연구에서는 4-5년 만에 형질이 고정되었음을 확인하였다.

• 식물조직배양학회지
• /
• 제27권4호
• /
• pp.309-315
• /
• 2000

Panax ginseng is an important medicinal plant that has been used worldwide for geriatric, tonic, stomachic, and aphrodisiac treatments. Ginsenosides contained in the ginseng root are the main substances having active functions for human body. The price of ginseng is very expensive due to a complex process of cultivation, and the yield of ginseng is limited, which cannot meet the demand of the increasing market. Researchers have applied plant biotechnology to solve the problems but there are still things to be determined towards ginsenoside production by large-scale adventitious root culture. In this experiment, 5 to 20 liter bioreactors were employed to determine optimal conditions for adventitious root culture and ginsenoside production of Panax gineng. Callus was induced from the ginseng root on MS agar medium containing 1.0 mg. $L^{-1}$ 2,4-D and 0.1 mg. $L^{-1}$ kinetin. Then the callus was cultured on MS agar medium supplemented with 2.0 mg. $L^{-1}$ IBA, 0.1 mg. $L^{-1}$ kinetin, and 30 g. $L^{-1}$ to induce adventitious roots. The maximum root growth and ginsenoside production were obtained in 1/2 MS medium. 2.0 mg. $L^{-1}$ naphthalene acetic acid resulted in greater root growth than 2.0 mg $L^{-1}$ indole-3-butyric acid. Ginsenoside content increased with 2.0 mg. $L^{-1}$ benzyl adenin or kinetin. High concentrations of benzyl adenin (above 3.0 mg. $L^{-1}$ ) decreased the adventitious root growth and ginsenoside productivity. N $H_{4}$$^{+}$ inhibited the ginsenoside accumulation, while high concentrations of $K^{+}$, $Mg_{2}$$^{+}$, and $Ca_{2}$$^{+}$ increased it. N $H_{4}$$^{+}$ at 0.5 and 1.0 times of the normal amount in 3/4 SH medium resulted in the greatest biomass increase, but the highest ginsenoside productivity was obtained when N $O_{3}$$^{-}$ was used as the sole nitrogen source in the medium. Most microelements at high concentrations in the medium inhibited the root growth, but high concentrations of MnS $O_4$enhanced the root growth. Root dry weight increased with increasing sucrose concentrations up to 50 g. $L^{-1}$ , but decreased from 70 g $L^{-1}$ Ginsenoside productivity was maximized at the range of 20 to 30 g. $L^{-1}$ sucrose. In the experiment on bioreactor types, cone and balloon types were determined to be favorable for both adventitious root growth and ginsenoside production. Jasmonic acid was effective for increasing ginsenoside contents and Rb group ginsenosides mainly increased. These results could be employed in commercial scale bioreactor cultures of Panax ginseng.x ginseng.

• Journal of Plant Biotechnology
• /
• 제4권3호
• /
• pp.103-107
• /
• 2002

The Good Agriculture Practice (GAP) program, being established in China, is an optimal way for the sustainable utilization of the medicinal plant and animal resources. Most frequently used Chinese materia medica will be mainly produced from the GAP bases in the future. To assure the successful operation of GAP program, standard operating procedure (SOP) should be implemented for specific plants or animals. Both GAP and SOP include the requirements in many aspects from the ecological environment of cultivation place, germplasm and varieties, seedling and transplant, fertilization, irrigation, and field care, to harvest and process, package, transport and storage. As a complex system, GAP demands strong commitment from the pharmaceutical industry, local administrative involvement, long term R&D support, and years of time of development before a satisfactory result can be achieved.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제6권1호
• /
• pp.18-24
• /
• 2001

The advantage of using Streptomyces griseus HUT 6037 in the production of chitinase or chitosanase is that the organism is capable of hydrolyzing amorphous or crystalline chitin and chitosan according to the type of the substrate used. We investigated the effects of the enzyme induction time and chitin sources, CM-chitosan and deacetylated chitosan (degree of deacetylation 75-99%), on production of chitosanase. We found that this strain accumulated chitosanase when cells were grown in the culture medium containing chitosanaceous substrates instead of chitinaceous substrates. The highest chitosanase activity was obtained at 4 dyas of cultivation with 99% deacetylated chitosan. The specific activities of chitinase and chitosanase were 0.91 and 1.33 U/mg protein at 3 and 5 days, respectively. From the study of the enzymatic digestibility of various degrees of deacetylated chitosan, it was found that (GlcN)$_3$, (GlcN)$_4$and (GlcN)(sub)5 were produced during the enzymatic hydrolysis reaction. The results of this study suggested that the sugar composition of (GlcN)$_3$was homogeneous and those of (GlcN)$_4$and (GlcN)(sub)5 were heterogeneous.

• 공학교육연구
• /
• 제9권1호
• /
• pp.61-74
• /
• 2006

본 연구에서는 체계적인 공학 설계 교육을 위한 입문 과정으로 개발된 '입문공학설계' 교과목의 내용과 운영 방식을 논의하였다. 이 교과목의 목적은 후속 설계 과목 학습에 필요한 기본 설계 소양을 갖추게 하고 공학에 대한 긍정적인 호기심을 자극하기 위한 것이다. 이를 위하여 본 수업은 학생들의 능동적인 참여로 이루어지는 수업을 기본 교수 방법으로 하여 창의적 사고 능력과 팀웍기술, 의사소통기술 배양을 위한 각종 설계 및 실습 과제를 개발하였다.

• Journal of Microbiology and Biotechnology
• /
• 제13권4호
• /
• pp.578-583
• /
• 2003

The desulfurization genes (dszABC) were cloned from Gordonia nitida. Nucleotide sequences similarity between the dszABC genes of G. nitida and those of Rhodococcus rhodochrous IGTS8 was 89%. The similarities of deduced amino acids between the two were 86% for DszA, 86% for DszB, and 90% for DszC. The G. nitida dszABC genes were expressed in several different Escherichia coli strains under an inducible trc promoter. Cultivation of these metabolically engineered E. coli strains in the presence of 0.2 mM dibenzothiophene (DBT) allowed the conversion of DBT to 2-hydroxybiphenyl (2-HBP), which is the final metabolite of the sulfur-specific desulfurization pathway. The maximum conversion of DBT to 2-HBP was 16% in 60 h. Recombinant E. coli was applied for the deep desulfurization of diesel oil supplemented into the medium at 5% (v/v). Sulfur content in diesel oil was decreased from 250 mg sulfur/1 to 212.5 mg sulfur/1, resulting in the removal of 15% of sulfur in diesel oil in 60 h.