• Title/Summary/Keyword: Adipate

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Utilization of cyclohexanol and characterization of Acinetobacter calcoaceticus C-15 (Acinetobacter calcoaceticus C-15에 의한 Cyclohexanol의 이용 및 그 특성)

  • Kim, Kyung Ae;Park, Jong Sung;Rhee, In Koo
    • Microbiology and Biotechnology Letters
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    • v.13 no.1
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    • pp.71-77
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    • 1985
  • A bacterium which grows on cyclohexanol as sole carbon and energy source was isolated from sludge of industrial areas in Taegu and identified as Acinetobacter calcoaceticus C-15. The growth medium for the optimal culture condition was composed of 0.2% cyclohexanol, 0.11% $NH_4Cl$, 0.05% $KH_2PO_4$, 0.2% $K_2HPO_4$, 0.02% $MgSO_4{\cdot}7H_2O$, and 0.05% yeast extracts. The optimal pH value and temperature for the growth were 7.2 and $33^{\circ}C$, respectively. Specific growth rate of A. calcoaceticus C-15 at $33^{\circ}C$ on the cyclohexanol and cyclohexanone was $0.27hr^{-1}$ and $0.15hr^{-1}$, respectively. Growth yield for cyclohexanol was 1.0. The bacteria utilized ethanol, 1-butanol, 1-pentanol, and cyclohexanol as a carbon source but not methanol, 1-hexanol, m-cresol, glycerol, and cyclohexane. The bacteria grew on benzoate, adipate, acetate, and citrate, but did not on salicylate, phthalate, p-hydroxybenzoate, and gluconate. A calcoaceticus C-15 did not utilize all kind of sugars other than xylose. Cell-free extracts contained $NAD^+$-linked cyclohexanol dehydrogenase which catalized the oxidation of cyclohexanol to cyclohexanone.

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Isolation and Characteristics of ${\varepsilon}$-Caprolactam Utilizing Bacteria (${\varepsilon}$-Caprolactam 이용성(利用性) 세균(細菌)의 분리(分離) 및 그 성질(性質))

  • Choi, Sun Taek;Rhee, In Koo
    • Current Research on Agriculture and Life Sciences
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    • v.3
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    • pp.21-27
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    • 1985
  • A bacterium which utilizes ${\varepsilon}$-caprolactam as a sole source of carbon and nitrogen was isolated from sludge of Shinchun river in Taegu and identified as Arthrobacter globiformis N-2-l. The growth medium for the optimum culture condition was composed of 0.4% ${\varepsilon}$-caprolactam, 0.02% $K_2HPO_4$, 0.05% $KH_2PO_4$, 0.02% $MgSO_4{\cdot}7H_2O$, 0.01% $FeCl_3{\cdot}6H_2O$ and 0.05% yeast extract. The optimum pH and temperature for growth were 7.0 and $30^{\circ}C$ respectively. The bacterial growth on the ${\varepsilon}$-caprolactam medium did not require any other organic nitrogen source such as yeast extract, although it was remarkably stimulated by the yeast extract. The bacteria utilized wide range of sugars and organic acids such as ${\alpha}$-ketoglutarate, adipate and P-hydroxybenzoate. The bacteria could use all kind of amino acids, ${\varepsilon}$-Caprolactam in the medium was consumed completely in the timecourse culture at $30^{\circ}C$ for 60 hr on the shaker by the bacteria. Decomposition product of ${\varepsilon}$-caprolactam by Arthrobacter globiformis N-2-1 was ${\varepsilon}$-aminocaproic acid.

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Preparation and Physical Properties of Eco-Friendly Biodegradable PLA/PBAT/HCO Blended Films (친환경 생분해성 PLA/PBAT/HCO 블랜드 필름 제조 및 물리적 특성)

  • Lee, Seung-Min;Kim, Han-Seong;Yun, Yeon-Hum;Hyung, Tae-Gyung;Yoon, Soon-Do
    • Applied Chemistry for Engineering
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    • v.31 no.4
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    • pp.416-422
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    • 2020
  • In this study, eco-friendly biodegradable materials were prepared using poly(lactic acid) (PLA), poly(butylene adipate-co-terephthalate) (PBAT), and hydrogenated castor oil power (HCO) as an additive. The prepared PLA/PBAT/HCO blended films were characterized by the scanning electron microscope (SEM) and fourier-transform infrared spectroscopy (FT-IR). The results of SEM analysis indicated that PLA/PBAT (8 : 2) blended films added HCO showed no rough area, crack, or large agglomeration when compared with those adding various additives (12-hydroxy stearic acid (12HSA) and cellulose). The FT-IR results indicated the presence of specific peak of HCO in the PLA/PBAT blended films, and its peak intensity increased with increasing HCO content (0~5.0 wt%). Tensile strength, elongation at break, and water barrier and thermal properties of the prepared PLA/PBAT/HCO blended films were also investigated, indicating that the physical and thermal properties was improved more than three times by the addition of HCO. The biodegradability test in soil revealed that the prepared biodegradable materials were degraded by about 6.0~20% after 90 days.

Optimization of mixing ratio in preparation of gluten-free rice udon through response surface methodology (반응 표면 분석법을 이용한 글루텐 프리 쌀 우동 제조 최적화)

  • Park, Se-Jin;Eun, Jong-Bang
    • Korean Journal of Food Science and Technology
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    • v.53 no.6
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    • pp.739-748
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    • 2021
  • This study focuses on the use of rice in the production of gluten-free rice udon (GFU) through an optimized mixing ratio, using the Box-Behnken response surface methodology (RSM). Different additional levels of rice flour (A, 40-60 g), acetylated distarch adipate (B, 10-20 g), and trehalose (C, 0-3 g) were used as variables, while water absorption level, volume, cooking loss, solid yield, lightness, texture properties, proximate compositions of GFU and turbidity of cooking water were set as responses in the RSM design model. The optimum mixing ratio for the preparation of gluten-free rice udon was obtained for 60.00 g of rice flour, 18.81 g of acetylated distarch adipate without the addition of trehalose. The response values of the optimized samples were water absorption (60.94%), volume (34.94%), turbidity of the cooking water (0.37), cooking loss (4.77%), solid yield (1.55 g), lightness value (70.04), hardness (2.53 N), springiness (0.18), gumminess (10.45 N), chewiness (1.83 N), and cohesiveness (2.89). This study has shown that rice flour can replace wheat flour to manufacture udon at an optimized mixing ratio successfully derived by statistical estimation method.

Thermal Properties of Corn-Starch Filled Biodegradable Polymer Bio-Composites (옥수수 전분을 충전제로 첨가한 생분해성 고분자 복합재료의 열적성질)

  • Kim, Hee-Soo;Yang, Han-Seung;Kim, Hyun-Joong;Lee, Young-Kyu;Park, Hee-Jun
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.5
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    • pp.29-38
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    • 2004
  • In this study, we investigated the thermal properties of corn-starch filled polybutylene succinate-adipate (PBS-AD) bio-composites. Thermal analysis (TA) is used to describe the analytical method for measuring the chemical property and weight loss of composite materials as a function of temperature. The thermal stability of corn-starch was lower than that of pure PBS-AD. As corn-starch loading increased, the thermal stability and degradation temperature of the bio-composites decreased and the ash content increased. It can be seen that the degree of compatibility and interfacial adhesion of the bio-composites decreased because of the increasing mixing ratio of the corn-starch. As the content of corn-starch increased, there was no significant change in the glass transition temperature (Tg) and the melting temperature (Tm) for the bio-composites. The storage modulus (E') and loss modulus (E") of the corn-starch flour filled PBS-AD bio-composites were higher than those of PBS-AD, because of the incorporation of corn-starch increased the stiffness of the bio-composites. At higher temperatures, the decreased storage modulus (E') of bio-composites was due to the increased polymer chain mobility of the matrix polymer. From these results, we can expect that corn-starch has potential as a reinforcing filler for bio-composites. Furthermore, we recommend using a coupling agent to improve the interfacial adhesion between corn-starch and biodegradable polymer.

Effect of Chemical Foaming Process on the Cellular Structure Development and Correlation with the Mechanical and Physical Property of PBAT (화학적 발포 공정이 PBAT 발포 셀 구조 발달에 미치는 영향과 기계적, 물리적 특성과의 상관관계 연구)

  • Yeong ho Ji;Tae Hyeong Park;Ji Eun Choo;Sung Wook Hwang
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.30 no.1
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    • pp.63-72
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    • 2024
  • Poly (butylene adipate-co-terephthalate) (PBAT) is one of the representative biodegradable polymers with high ductility and processability to replace petroleum-based polymers. Many investigations have been conducted to broaden the applications of PBAT in a variety of industries, including the food packaging, agricultural mulching film, and logistics and distribution fields. Foaming process is widely known technique to generate the cell structure within the polymer matrix, offering the insulation and light weight properties. However, there was no commercially feasible foam product based on biodegradable polymers, especially PBAT, and maintaining a proper melt viscosity of the polymer would be a key parameter for the foaming process. In this study, chemical foaming agent and cross-linking agent were introduced to PBAT, and a compression molding process was applied to prepare a foam sheet. The correlation between cell morphological structures and mechanical and physical properties was evaluated. It was found that PBAT with foam structures effectively reduced the density and thermal conductivity, allowing them to be suitable for applications such as insulation and lightweight packaging or cushion materials.

Development of the submerged heat treatment machine for PBSAT(polybutylene succinate adipate-co-terephthalate) monofilament nets and its efficiency (수중 침지식 생분해성 PBSAT 그물 열처리기 개발과 성능 분석)

  • Park, Seongwook;Kim, Seonghun;Lim, Jihyun;Choi, Haesun
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.51 no.1
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    • pp.94-101
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    • 2015
  • The heat treatment machine based on immersion was developed to reduce temperature difference during netting process and appraised it performance compared current heat treatment machine using high pressure. It was also reviewed the optimum heat treatment procedures for PBSAT monofilament net in accordance with the immersion time and temperature. The procedure was based on physical measurement such as breaking load, elongation and angle of the mesh for PBSAT monofilament. The water temperature gap of the treatment machine based on immersion was less than $1^{\circ}C$. and the energy consumption was also increased in high temperature condition. It was identified that the optimum temperature was $75^{\circ}C$ and its optimum processing time was between 15 minutes and 20 minutes to get qualified physical properties.

Study on Phase Separation of Carbon Dioxide-reducible Polymer Blends (이산화탄소 저감형 고분자 블렌드의 상 분리 특성연구)

  • Cho, Yong-Kwang;Kim, Yeong-Woo;Lee, Hak Yong;Park, Sang-Bo;Park, Chan-Young;Lee, Won-Ki
    • Journal of Environmental Science International
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    • v.24 no.1
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    • pp.9-15
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    • 2015
  • Sustainable and eco-friendly polymers, natural polymers, bio-based polymers, and degradable polyesters, are of growing interest because of environmental concerns associated with waste plastics and emissions of carbon dioxide from preparation of petroleum-based polymers. Degradable polymers, poly(butylene adipate-co-terephthalate) (PBAT), poly(propylene carbonate) (PPC), and poly(L-lactic acid) (PLLA), are related to reduction of carbon dioxide in processing. To improve a weak mechanical property of a degradable polymer, a blending method is widely used. This study was forced on the component separation of degradable polymer blends for effective recycling. The melt-mixed blend films in a specific solvent were separated by two layers. Each layer was analysed by FT-IR, DSC, and contact angle measurements. The results showed that each component in the PPC/PLLA and PPC/PBAT blends was successfully separated by a solvent.

Metabolic profiling study of ketoprofen-induced toxicity using 1H NMR spectroscopy coupled with multivariate analysis

  • Jung, Jee-Youn;Hwang, Geum-Sook
    • Journal of the Korean Magnetic Resonance Society
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    • v.15 no.1
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    • pp.54-68
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    • 2011
  • $^1H$ nuclear magnetic resonance (NMR) spectroscopy of biological samples has been proven to be an effective and nondestructive approach to probe drug toxicity within an organism. In this study, ketoprofen toxicity was investigated using $^1H$-NMR spectroscopy coupled with multivariate statistical analysis. Histopathologic test of ketoprofen-induced acute gastrointestinal damage in rats demonstrated a significant dose-dependent effect. Furthermore, principal component analysis (PCA) derived from $^1H$-NMR spectra of urinary samples showed clear separation between the vehicle-treated control and ketoprofen-treated groups. Moreover, PCA derived from endogenous metabolite concentrations through targeted profiling revealed a dose-dependent metabolic shift between the vehicle-treated control, low-dose ketoprofen-treated (10 mg/kg body weight), and high-dose ketoprofen-treated (50 mg/kg) groups coinciding with their gastric damage scores after ketoprofen administration. The resultant metabolic profiles demonstrated that the ketoprofen-induced gastric damage exhibited energy metabolism perturbations that increased urinary levels of citrate, cis-aconitate, succinate, and phosphocreatine. In addition, ketoprofen administration induced an enhancement of xenobiotic activity in fatty oxidation, which caused increase levels of N-isovalerylglycine, adipate, phenylacetylglycine, dimethylamine, betaine, hippurate, 3-indoxylsulfate, N,N-dimethylglycine, trimethyl-N-oxide, and glycine. These findings demonstrate that $^1H$-NMR-based urinary metabolic profiling can be used for noninvasive and rapid way to diagnose adverse drug effects and is suitable for explaining the possible biological pathways perturbed by nonsteroidal anti-inflammatory drug toxicity.

The Effect of Modified Starch(Acetylated Distarch Adipate) on the Quality Characteristics of Jeungpyun (변성전분을 첨가한 증편의 특성)

  • Lee, Kun-Jong;Choi, Bong-Soon;Kim, Hye-Young
    • The Korean Journal of Community Living Science
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    • v.23 no.3
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    • pp.233-243
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    • 2012
  • This study was performed to determine the quality characteristics of Jeungpyun with added modified starch. The addition of modified starch did not change the pH level of the samples, but it did increase the volume. The b-value of Jeungpyun with 3% modified starch was significantly lower than that of the control group. The addition of 3% modified starch also significantly increased the hardness, adhesiveness, gumminess and chewiness of the experimental group over the control group. In the test of sensory characteristics, the cell size of Jeungpyun with 2 and 3% modified starch was larger than that of the control group. Cell uniformity of Jeungpyun with 2% modified starch was lower than that of the control group. The hardness of Jeungpyun with modified starch was significantly higher than that of the control group. In the test of taste preference, Jeungpyun with modified starch beat the control group substantially. The quality of Jeungpyun with 1 and 3% modified starch showed a higher overall score than that of the control group.