• Journal of Animal Reproduction and Biotechnology
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• v.38 no.3
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• pp.121-130
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• 2023

Background: Coating a culture plate with molecules that aid in cell adhesion is a technique widely used to produce animal cell cultures. Extracellular matrix (ECM) is known for its efficiency in promoting adhesion, survival, and proliferation of adherent cells. Gelatin, a cost-effective type of ECM, is widely used in animal cell cultures including feeder-free embryonic stem (ES) cells. However, the optimal concentration of gelatin is a point of debate among researchers, with no studies having established the optimal gelatin concentration. Methods: In this study, we coated plastic plates with gelatin in a concentration-dependent manner and assessed Young's modulus using atomic force microscopy (AFM) to investigate the microstructure of the surface of each plastic plate. The adhesion, proliferation, and differentiation of the ESCs were compared and analyzed revealing differences in surface microstructure dependent on coating concentration. Results: According to AFM analysis, there was a clear difference in the microstructure of the surface according to the presence or absence of the gelatin coating, and it was confirmed that there was no difference at a concentration of 0.5% or more. ES cell also confirmed the difference in cell adhesion, proliferation, and differentiation according to the presence or absence of gelatin coating, and also it showed no difference over the concentration of 0.5%. Conclusions: The optimum gelatin-coating for the maintenance and differentiation of ES cells is 0.5%, and the gelatin concentration-mediated microenvironment and ES cell signaling are closely correlated.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2010.05a
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• pp.717-718
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• 2010

• Transactions of the Korean Society of Mechanical Engineers B
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• v.36 no.7
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• pp.721-730
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• 2012

The present paper presents a numerical study on multiphase flows induced by wall adhesion. The continuum surface force (CSF) model with the wall adhesion boundary condition model is used for calculating the surface tension force; this model is implemented in an in-house solution code (PowerCFD). The present method (code) employs an unstructured cell-centered method based on a conservative pressure-based finite-volume method with a volume capturing method (CICSAM) in a volume of fluid (VOF) scheme for phase interface capturing. The effects of wall adhesion are then numerically simulated by using the present method for a shallow pool of water located at the bottom of a cylindrical tank with no external forces such as gravity. Two different cases are computed, one in which the water wets the wall and one in which the water does not wet the wall. It is found that the present method efficiently simulates the surface tension-dominant multiphase flows induced by wall adhesion.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.2
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• pp.150-156
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• 2006

It is one of the most effective methods for improving the performance of electric railway vehicles to make better the wheel-rail adhesion characteristics. To study adhesion characteristic is to develop the equivalent reduction machine to experiment on the adhesion system. The experiment system makes it possible to change the wheel-rail adhesion force with various adhesion parameters, and therewith to test the adhesion control system with the reduction machine in a laboratory. In this paper, for improving adhesion performance shows actually control methods.

• Journal of Institute of Control, Robotics and Systems
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• v.11 no.6
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• pp.502-509
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• 2005

In this paper, a 4-wheel vehicle model including the effects of tire slip was considered, along with variable parameter sliding control, in order to improve the performance of the vehicle longitudinal response. The variable sliding parameter is made to be proportional to the square root of the pressure derivative at the wheel, in order to compensate for large pressure changes in the brake cylinder. A typical tire force-relative slip curve for dry road conditions was used to generate an analytical tire force-relative slip function, and an antilock sliding control process based on the analytical tire force-relative slip function was used. A retrofitted brake system, with the pushrod force as the end control parameter, was employed, and an average decay function was used to suppress the simulation oscillations. The simulation results indicate that the velocity and spacing errors were slightly larger than those obtained when the wheel slip effect was not considered, that the spacing errors of the lead and follower were insensitive to the adhesion coefficient up to the critical wheel slip value, and that the limit for the antilock control under non-constant adhesion road conditions was determined by the minimum value of the equivalent adhesion coefficient.

• Laser Solutions
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• v.6 no.3
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• pp.29-35
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• 2003

In semiconductor processing, there are two types of particle contaminated onto the wafer, i.e. dry and wet state particles. In order to evaluate the cleaning performance of laser shock wave cleaning method, the removal of 1 m sized alumina particle at different particle conditions from silicon wafer has been carried out by laser-induced shock waves. It was found that the removal efficiency by laser shock cleaning was strongly dependent on the particle condition, i.e. the removal efficiency of dry alumina particle from silicon wafer was around 97％ while the efficiencies of wet alumina particle in DI water and IPA are 35％ and 55％ respectively. From the analysis of adhesion forces between the particle and the silicon substrate, the adhesion force of the wet particle where capillary force is dominant is much larger than that of the dry particle where Van der Waals force is dominant. As a result, it is seen that the particle in wet condition is much more difficult to remove from silicon wafer than the particle in dry condition by using physical cleaning method such as laser shock cleaning.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 2002.05a
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• pp.207-214
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• 2002

Nano adhesion between SPM (scanning probe microscope) tips and $DDPO_{4}$ (dodecylphosphoric acid ester) and $ODPO_{4}$ (octadecylphosphoric acid ester) SAM (self-assembled monolayer) was experimentally studied. Tests were performed to measure the nano adhesion and friction in both AFM(atomic force microscope) and LFM(lateral force microscope) modes with the applied normal load. $DDPO_{4}$ and $ODPO_{4}$ SAM were formed on TiMe and TiOx surfaces. TiMe and TiOx were coated on the Si wafer by ion sputtering. Adhesion and friction of $DDPO_{4}$ and $ODPO_{4}$ SAM surfaces were compared with those of OTS (octadecyltrichlorosilane) SAM and DLC surfaces. $DDPO_{4}$ and $ODPO_{4}$ SAM converted the TiMe and TiOx surfaces to be hydrophobic. When the surface was hydrophobic, the adhesion and friction forces were found lower than those of bare surfaces. Work of adhesion was also discussed to explain how the surface was converted into hydrophobic. Results also showed that tribological characteristics of $DDPO_{4}$ and $ODPO_{4}$ had good properties in the adhesion, friction, wetting angle and work of adhesion. $DDPO_{4}$ and $ODPO_{4}$ SAM could be one of the candidates for the bio-MEMS elements.

• Proceedings of the KIEE Conference
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• 2000.07b
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• pp.1436-1438
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• 2000

It is one of the most effective methods for improving the performance of electric railway vehicles to make better the wheel-rail adhesion characteristics. To study adhesion characteristic is to develop the equivalent reduction machine to experiment on the adhesion system. The experiment system makes it possible to change the wheel-rail adhesion force with various adhesion parameters, and therewith to test the adhesion control system with the reduction machine in a laboratory. In this paper, for improving adhesion performance shows actually control methods.

• Journal of Adhesion and Interface
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• v.3 no.1
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• pp.43-51
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• 2002

Advanced adhesive technologies and demanding applications of adhesive joints can no longer be developed successfully by the traditional "trial and error" approach. Appropriate technical solutions require reference to a reliable basis of well-established scientific knowledge about the elementary mechanisms of adhesion (i.e. the 'fundamental adhesion') as they are responsible for the capability of the compound w transmit mechanical force between the adhesive and the substrate surface (i.e. the 'practical adhesion'). Adhesion mechanisms also influence the formation of polymer structure in the adhesive and the resulting macromolecular dynamics in the interphase that is formed in the adhesive near to the substrate. These manifold molecular factors rule the macroscopic behaviour of an adhesive bond line in terms of mechanical and other physical properties as well as in terms of durability. This paper reviews the level of refinement that understanding of fundamental adhesion has achieved up to now.

• Proceedings of the KSR Conference
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• 2006.11b
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• pp.659-664
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• 2006

It is one of the most effective method for improving the performance of electric railway vehicles to make better the wheel-rail adhesion characteristics. To study adhesion characteristic is to develop the equivalent reduction machine to experiment on the adhesion system. The experiment system makes it possible to change the wheel-rail adhesion force with various adhesion parameters, and therewith to test the adhesion control system with the reduction machine in a laboratory. In this paper for improving adhesion performance show actually control methods.