• Natural Product Sciences
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• v.23 no.1
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• pp.29-34
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• 2017

In this study, we investigated whether adenosine, adenine, uridine and homogentisic acid derived from Pinellia ternata affect the secretion, production and gene expression of MUC5AC mucin from airway epithelial cells. Confluent NCI-H292 cells were pretreated with adenosine, adenine, uridine or homogentisic acid for 30 min and then stimulated with PMA (phorbol 12-myristate 13-acetate) for 24 h. The MUC5AC mucin gene expression, mucin protein production and secretion were measured by RT-PCR and ELISA, respectively. The results were as follows: (1) Adenine and homogentisic acid decreased PMA-induced MUC5AC mucin gene expression, although adenosine and uridine did not affect the mucin gene expression; (2) Adenosine, adenine, uridine and homogentisic acid inhibited PMA-induced MUC5AC mucin production; (3) Homogentisic acid inhibited the secretion of MUC5AC mucin from NCI-H292 cells. These results suggest that, among the four compounds examined, homogentisic acid showed the regulatory effect on the steps of gene expression, production and secretion of mucin, by directly acting on airway epithelial cells.

• Proceedings of the KAIS Fall Conference
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• 2011.12a
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• pp.357-360
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• 2011

A new copper adenine MOF (Bio-MOF) was synthesized by hydrothermal procedure and explored for its low temperature $CO_2$ adsorption. In this adenine a DNA nucleotide was used as a ligand for Cu in DMF solution at $130^{\circ}C$. The synthesized Bio MOF was characterized by XRD, SEM, EDS, TG and BE Tresults. The material possesses high surface area (716.08 $m^2g^{-1}$) with mono dispersed particles of about 2.126 nm. The maximum $CO_2$ adsorption capacity is 5wt% at $50^{\circ}C$, which is regenerable at $100^{\circ}C$ which is very low when compared to other metal organic frame work studied. This study proves that the synthesized material is also be a choice materials for low temperature $CO_2$adsorption.

• Molecules and Cells
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• v.42 no.6
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• pp.495-500
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• 2019

Brain cytoplasmic 200 RNA (BC200 RNA), originally identified as a neuron-specific non-coding RNA, is also observed in various cancer cells that originate from non-neural cells. Studies have revealed diverse functions of BC200 RNA in cancer cells. Accordingly, we hypothesized that BC200 RNA might be modified in cancer cells to generate cancerous BC200 RNA responsible for its cancer-specific functions. Here, we report that BC200 RNA sequences are highly heterogeneous in cancer cells by virtue of multiple adenine nucleotide insertions in the internal A-rich region. The insertion of adenine nucleotides enhances BC200 RNA-mediated translation inhibition, possibly by increasing the binding affinity of BC200 RNA for eIF4A (eukaryotic translation initiation factor 4A).

• Journal of Plant Biotechnology
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• v.2 no.1
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• pp.21-24
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• 2000

An efficient method of shoot regeneration of peanut is described. In vitro shoot organogenesis from the callus of cotyledon explants of Arachis hypogaea L. was stimulated by addition of Adenine sulphate (Ads) along with 6 - benzylaminopurine (BAP) and - napthalene acetic acid (NAA). Ads (13 ${\mu}{\textrm}{m}$) had a stimulatory effect on shoot bud differentiation when combined with BAP (13 ${\mu}{\textrm}{m}$) and NAA (2 ${\mu}{\textrm}{m}$). Shoot organogenesis was markedly higher (92%) from callus induced on Ads, BAP and NAA combined media than from those formed by the individual supplementation of Ads or BAP with NAA. The shoots elongated on the media with GA$_3$ (1 ${\mu}{\textrm}{m}$). Elongated plantlets rooted with MS media containing IBA (9 ${\mu}{\textrm}{m}$).

• Bulletin of the Korean Chemical Society
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• v.31 no.8
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• pp.2180-2184
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• 2010

We synthesized and tested the anti-HIV activity of the SATE prodrug of a 2'-methyl 5'-norcarbocyclic adenine analogue. The introduction of a methyl group in the 2'-position was performed by the addition of a carbonyl using isopropenyl magnesium bromide. The adenine base was efficiently coupled using the Mitsunobu reaction. The chemical stability study of the bis(SATE) derivative 18 was measured at neutral (pH 7.2) and slightly acid (milli-Q water, pH 5.5) pH, and compounds 16 and 18 were evaluated as potential anti-HIV-1 agents.

• YAKHAK HOEJI
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• v.28 no.2
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• pp.101-127
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• 1984

The study of interaction between riboflavin derivatives and biologically active substances was reviewed. With combination of spectroscopic methods such as NMR, UV, Fluorescence and IR, informations about interaction mechanism including hydrogen bond formation, conformation of association complex, and association constant were obtained. 1. Riboflavin associated with adenine but not with other bases found in the nucleic acids. -CONHCO- group was included in the formation of hydrogen bond with adenine. 2. Riboflavin interacted with alcohol to make a 1 : 1 association complex through the 3N-imino and 2C-carbonyl group of the isoalloxazine ring and the hydroxyl group of the alcohols. 3. Riboflavin associated with salicylates to produce the cyclic hydrogen-bonded dimer. The strongest complex was formed with salicylic acid, a weaker one with aspirin, and an even weaker one with salicylamide. 4. Other bio-active substances, orotic acid and inhibitors such as phenol, trichloroacetic acid and indol also formed hydrogen bond with riboflavin. 5. Reduced riboflavin showed strong self-association to produce the cyclic hydrogen-bonded complex and it associated with adenine and with cytosine to form 1 : 3 complex.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.274.1-274.1
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• 2003

To elucidate the effect of bioconversioned ginseng(Sun ginseng) and its butanol fraction on adenine-induced renal failure. rats were fed ad libitum on diet containing 0.75％ adenine for 20 days to induce renal failure, and bioconversioned ginseng was orally administrated during the feeding period. On days 10 and 20, BUN, Creatinine, Ca and P contents were analyzed in serum and urine, and on days 20, BUN, Creatining, Ca and P contents were analyzed in serum and urine, and on days 20, blood pressure, hear pulse and relative kidney weight were measured. (omitted)

• Korean Journal of Exercise Nutrition
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• v.16 no.1
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• pp.35-41
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• 2012

We investigated the role of fatty acid availability on skeletal muscle AMPK activity and adenine nucleotide content. To investigate the chronic effects of elevated fatty acid in vivo Sprague-Dawley rats were fed a chow diet (15% fat) or a diet high in saturated (SAFA, 52% fat) or polyunsaturated (PUFA, 52% fat) fat for eight weeks. High fat diets increased (P < 0.05) plasma FFA levels by 25%. AMPK activity was increased in SAFA and PUFA rats and occurred in the absence of changes in ATP, AMP, phosphocreatine and glycogen content. These results suggest that increasing fatty acid availability increases AMPK activity independent of changes in the cellular energy charge, and implicate the regulation of AMPK by a covalent mechanism. These data also support the contention that increasing fatty acid availability can increase subsequent fatty acid oxidation by an AMPK-mediated process.

• Korean Journal of Microbiology
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• v.25 no.3
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• pp.221-228
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• 1987

The extracellular adenine deaminase from Nocardioides sp. J-275L was purified by the following techniques: ammonium sulfate fractionation, DEAE-Cellulose, DEAE-Sephadex A-50 column chromatography, and Sephacryl S-200 superfine gel filtration. The enzyme was partially purified about 3889.5-fold with about 5.2% yield by these procedures. The molecular weight of the enzyme was 39,000 by a calibrated Sephacryl S-200 superfine column chromatography. The enzyme was stable at pH 7.5 and up to $40^{\circ}C$. Glycerol was effective on the stabilization of the enzyme during storage. The optimum pH and temperature of the enzyme were around pH 7.5 and $40^{\circ}C$, respectively. The apparent Michaelis constant Km of the enzyme for adenine was $7.4\times 10^{-5}$M. The purine analogues, 6-chloropurine, 2,6-diaminopurine, 6-bromopurine, 4-aminopyrazolo [3.4-d]pyrimidine, and 8-azaadenine were substrates for the enzyme. 6-Dimethylaminopurine was a competitive inhibitor of the enzyme. The enzyme was inhibited by 1mM of $Cu^{2+}, Fe^{3+}, Pb^{2+}, Hg^{2+}$, and $Ag^{+}$, and 1mM of $\alpha$,$\alpha$'-dipyridyl, pentachlorophenol, and pCMB.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.184-184
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• 1994

개와 흰쥐의 장간막동맥 절편의 긴장도와 $^{86}$Rb$^{+}$ 유출에 대한 cromakalim의 효과를 관찰하고 이를 ATP를 고갈시킨 조직에서 얻어지는 결과와 비교하였다. Cromakalim은 개와 흰쥐의 장간막 동맥을 이완시켰으며, 이러한 이완은 glibenclamide에 의하여 상경적으로 억제되었다. Glibenclamide는 phenylephriue에 의한 개와 흰쥐의 장간막 동맥 수축을 증가시킨다든지 혹은 cromakalim에 의한 억제성 반응을 역전시켰다. 개와 흰쥐 장간막 등맥의 $^{55}$Rb$^{+}$ 유출 실험에서 tromakalim (10 $\mu$M) 투여시 ATP를 고갈시킨 흰쥐의 장간막 동맥에서 현저히 증가하였다. HPLC를 이용하여 adenine nucleotide를 분석하였다. Adenine nucleotide의 HPLC 분석시에 혈관의 내피세포를 제거하였고 0.2 M ammonium phosphate (pH 5.5)를 용출용매로 이용하여 분석하였다.