• Nutrition Research and Practice
• /
• 제17권6호
• /
• pp.1056-1069
• /
• 2023

BACKGROUND/OBJECTIVES: Grifola frondosa, commonly referred to as the maitake mushroom, has been studied extensively to explore its potential health benefits. However, its anti-inflammatory effects in skin disorders have not been sufficiently elucidated. This study aimed to elucidate the anti-inflammatory role of the ethanol extract of G. frondosa in atopic dermatitis (AD) using in vivo and in vitro models. MATERIALS/METHODS: We investigated its impact on skin and spleen inflammatory responses in Dermatophagoides farinae extract (DFE)/1-chloro-2,4 dinitrochlorobenzene (DNCB)-induced AD-like skin lesions in a mouse model. Additionally, we determined the immunosuppressive response and mechanism of G. frondosa by inducing atopic-like immune reactions in keratinocytes through tumor necrosis factor (TNF)-α/interferon (IFN)-γ stimulation. RESULTS: Our study revealed that G. frondosa ameliorates clinical symptoms in an AD-like mouse model. These effects contributed to the suppression of Th1, Th2, Th17, and Th22 immune responses in the skin and spleen, leading to protection against cutaneous inflammation. Furthermore, G. frondosa inhibited the production of antibodies immunoglobulin (Ig)E and IgG2a in the serum of AD mice. Importantly, the inhibitory effect of G. frondosa on inflammatory cytokines in TNF-α/IFN-γ-stimulated AD-like keratinocytes was associated with the suppression of MAPK (Mitogen Activated Protein Kinase) pathway activation. CONCLUSIONS: Collectively, these findings highlight the potential of G. frondosa as a novel therapeutic agent for AD treatment and prevention.

• 정보보호학회논문지
• /
• 제30권5호
• /
• pp.871-879
• /
• 2020

Perceptual Ad-Blocking은 인공지능 기반의 광고 이미지 분류 모델을 이용하여 온라인 광고를 탐지하는 새로운 광고 차단 기법이다. 이러한 Perceptual Ad-Blocking은 최근 이미지 분류 모델이 이미지를 틀리게 분류하게 끔 이미지에 노이즈를 추가하는 적대적 예제(adversarial example)를 이용한 적대적 공격(adversarialbattack)에 취약하다는 연구 결과가 제시된 바 있다. 본 논문에서는 다양한 적대적 예제를 통해 기존 Perceptual Ad-Blocking 기법의 취약점을 증명하고, MNIST, CIFAR-10 등의 데이터 셋에서 성공적인 방어를 수행한 Defense-GAN과 MagNet이 광고 이미지에도 효과적으로 작용함을 보인다. 이를 통해 Defense-GAN과 MagNet 기법을 이용해 적대적 공격에 견고한 새로운 광고 이미지 분류 모델을 제시한다. 기존 다양한 적대적 공격 기법을 이용한 실험 결과에 따르면, 본 논문에서 제안하는 기법은 적대적 공격에 견고한 이미지 분류 기술을 통해 공격 이전의 이미지 분류 모델의 정확도와 성능을 확보할 수 있으며, 더 나아가 방어 기법의 세부사항을 아는 공격자의 화이트박스 공격(White-box attack)에도 일정 수준 방어가 가능함을 보였다.

• The Journal of Advanced Prosthodontics
• /
• 제15권6호
• /
• pp.290-301
• /
• 2023

PURPOSE. The study aimed to determine the influence of implant angulation on the trueness of multi-unit implant impressions taken through different techniques and strategies. MATERIALS AND METHODS. As reference models, three partially edentulous mandibular models (Model 1: No angulation; Model 2: No angulation for #33, 15-degree distal angulation for #35 and #37; Model 3: No angulation for #33, 25-degree distal angulation for #35 and #37) were created by modifying the angulations of implant analogues. Using a lab scanner, these reference models were scanned. The obtained data were preserved and utilized as virtual references. Three intraoral scanning (IOS) strategies: IOS-Omnicam, ISO-Quadrant, and IOS-Consecutive, as well as two traaditional techniques: splinted open tray (OT) and closed tray (CT), were used to create impressions from each reference model. The best-fit alignment approach was used to sequentially superimpose the reference and test scan data. Computations and statistical analysis of angular (AD), linear (LD), and 3D deviations (RMS) were performed. RESULTS. Model type, impression technique, as well as interaction factor, all demonstrated a significant influence on AD and LD values for all implant locations (P < .05). The Model 1 and SOT techniques displayed the lowest mean AD and LD values across all implant locations. When considering interaction factors, CT-Model 3 and SOT-Model 1 exhibited the highest and lowest mean AD and LD values, respectively. Model type, impression technique, and interaction factor all revealed significant effects on RMS values (P ≤ .001). CT-Model 3 and SOT-Model 1 presented the highest and lowest mean RMS values, respectively. CONCLUSION. Splinted-OT and IOS-Omnicam are recommended for multi-unit implant impressions to enhance trueness, potentially benefiting subsequent manufacturing stages.

• 대한의생명과학회지
• /
• 제23권4호
• /
• pp.303-309
• /
• 2017

${\beta}$-sitosterol, one of phytosterols, exhibited numerous pharmacological effect including anti-inflammatory, anti-cancer and immune-modulating properties. This study attempted to determine the pharmacological effects of ${\beta}$-sitosterol on atopic dermatitis (AD). We investigated to ascertain the pharmacological effects of ${\beta}$-sitosterol on 2, 4-dinitrochlrobenzene (DNCB)-induced AD symptom and histamine-induced scratching behaviors in mice. Additionally, we evaluated the effects of ${\beta}$-sitosterol on the interleukin (IL)-6 levels in HaCaT cells and skin tissue of AD. The findings of this study demonstrated that ${\beta}$-sitosterol reduced AD clinical symptoms such as eczematous, erythema and dryness and serum histamine and IgE levels in DNCB-induced AD model and histamine-induced scratching behaviors in mice. Additionally, ${\beta}$-sitosterol inhibited the IL-6 expression in AD-like skin lesion and HaCaT cells. Collectively, these findings provide that ${\beta}$-sitosterol could be a therapeutic agent for skin inflammation including AD.

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2003년도 통신소사이어티 추계학술대회논문집
• /
• pp.269-272
• /
• 2003

Ad hoc 네트워크에 대한 상업적인 관심이 지속적으로 증가함에 따라, 최근 Ad hoc 네트워크에 QoS (Quality of Service)를 지원하기 위한 많은 연구가 이루어지고 있다. 지금까지의 연구는 대부분 QoS 기반 라우팅 프로토콜과 자원 예약 (resource reservation) 그리고 QoS MAC (Medium Access Control) 프로토콜에 관한 것이었다. Ad hoc 네트워크에 QoS를 제공하기 위해서는 위의 요소들이 상호 협력하여 동작하고 Ad hoc 네트워크의 특성이 충분히 고려된 005 모델에 관한 연구가 절실히 필요하다. 본 논문에서는 Ad hoc 네트워크에서 종단 사용자간 (end-to-end) 005를 제공하기 위한 서비스 모델의 설계와 구현방법에 대해 제안하였다.

• 한방안이비인후피부과학회지
• /
• 제16권1호
• /
• pp.33-41
• /
• 2003

Infantile atopic dermatitis(AD) may be developed by food allergens due to immature intestinal epithelium and its mechanism, which may have no clear-cut evidence, is thought to be IgE mediated immediate and late phase hypersensitivity. It is not easy to diagnose AD caused by food allergens exactly so it is likely to be underestimated more than it be. But we must consider it as a major factor of not only infantile AD but childhood and adult AD. We can see similar theory at previous Oriental medicine. Allergens can be transmitted to a fetus through the placenta and infantile AD is inflammatory condition by food allergens and immature function of intestines. So we must consider those factors at infantile AD treatment. We expect a new model of infantile AD treatment combining the conventional therapy with the diet therapy based on the Oriental medical theory.

• Biomolecules & Therapeutics
• /
• 제28권6호
• /
• pp.542-548
• /
• 2020

Naturally derived diosmetin and its glycoside diosmin are known to be effective in treating inflammatory disease. This study was performed to determine whether diosmin and diosmetin have the effect of improving atopic dermatitis in a 2,4-dinitrochlorobenzen (DNCB)-induced atopic dermatitis (AD) model. DNCB was used to establish AD model in hairless mice. Skin moisture, serum immunoglobulin E (IgE), interleukin 4 (IL-4), and histological analysis were performed to measure the effectiveness of diosmin and diosmetine to improve AD. IL-4 levels were also measured in RBL-2H3 cells. Administration of diosmetin or diosmin orally inhibited the progress of DNCB-induced AD-like lesions in murine models by inhibiting transdermal water loss (TEWL) and increasing skin hydration. Diosmetin or diosmin treatment also reduced IgE and IL-4 levels in AD-induced hairless mouse serum samples. However, in the in vitro assay, only diosmetin, not diosmin, reduced the expression level of IL-4 mRNA in RBL-2H3 cells. Diosmin and diosmetine alleviated the altered epidermal thickness and immune cell infiltration in AD. Diosmin is considered effective in the cure of AD and skin inflammatory diseases by being converted into diosmetin in the body by pharmacokinetic metabolism. Thus, oral administration of diosmetin and diosmin might be a useful agent for the treatment of AD and cutaneous inflammatory diseases.

• Journal of Microbiology and Biotechnology
• /
• 제18권8호
• /
• pp.1393-1400
• /
• 2008

Recent study has demonstrated an increasing prevalence of food allergy in Korean children. Specific probiotic bacteria may promote potentially anti-allergenic processes through induction of Th1-type immunity and enhance the regulatory lymphocyte. This study investigated whether orally administrated probiotics could suppress allergic responses in an ovalbumin (OVA)-induced allergy mouse model. Thus, female C3H/HeJ mice were orally sensitized with OVA and cholera toxin for 4 weeks. Lactobacillus acidophilus AD031, Bifidobacterium lactis AD011, and L. acidophilus AD031 plus B. lactis AD011 were fed to mice from 2 weeks before the sensitization. The OVA-induced mice that were not treated with probiotics had significantly increased serum levels of OVA-specific IgE and IgG1, and OVA-specific IgA in feces. However, the mice treated with probiotics suppressed production of the OVA-specific IgE, IgG1, and IgA. The level of IL-4 was significantly lower, and the levels of INF-$\gamma$ and IL-10 were significantly higher in the mice treated with probiotics than that in the non-treated mice. The groups treated with probiotics had decreased levels of degranulated mast cells, eosinophil granules, and tail scabs. These results indicate that L. acidophilus AD031 and B. lactis AD011 might be useful for the prevention of allergy.

• 약학회지
• /
• 제56권2호
• /
• pp.92-98
• /
• 2012

This study was conducted to determine if YJ-1, a novel herbal complex from a mixture of six oriental herbs (Hydnocarpi Semen, Sesami Semen, Dictamni Radicis Cortex, Momordicae Semen, Xanthii Fructus, and Sophorae Radix), has therapeutic properties for the treatment of atopic dermatitis (AD). Using AD like symptom-induced NC/Nga mice by 1-chloro 2,4-dinitrobenzene (DNCB), the effectiveness of YJ-1 on AD was evaluated. Elidel cream$^{(R)}$ (1% pimecrolimus) was used as a control. Dermal application of YJ-1 reduced major clinical signs of AD such as erythema, pruritus, lichenification, edema/escoriations, and dryness. Interestingly, YJ-1 more improved AD-related symptoms including decrease of spleen weight, IL-4, and IgE level in the serum as well as reduction of scratching counts and clinical skin severity in the NC/Nga AD mouse model. Especially, treatment of YJ-1 at 20% in NC/Nga mice more effected than Elidel cream. These results suggest that the ointment of YJ-1 may enhance the process of AD healing by alleviating allergic reaction and has potential for therapeutic reagent for the treatment of AD.

• International Neurourology Journal
• /
• 제22권4호
• /
• pp.237-245
• /
• 2018

Purpose: Presenilins are functionally important components of ${\gamma}$-secretase, which cleaves a number of transmembrane proteins. Manipulations of PSEN1 and PSEN2 have been separately studied in Alzheimer disease (AD) and cancer because both involve substrates of ${\gamma}$-secretase. However, numerous clinical studies have reported an inverse correlation between AD and cancer. Interestingly, AD is a neurodegenerative disorder, whereas cancer is characterized by the proliferation of malignant cells. However, this inverse correlation in the PSEN double-knockout (PSEN dKO) mouse model of AD has been not elucidated, although doing so would shed light onto the relationship between AD and cancer. Methods: To investigate the inverse relationship of AD and cancer under conditions of PSEN loss, we used the hippocampus of 7-month-old and 18-month-old PSEN dKO mice for a microRNA (miRNA) microarray analysis, and explored the tumorsuppressive or oncogenic role of differentially-expressed miRNAs. Results: The total number of miRNAs that showed changes in expression level was greater at 18 months of age than at 7 months. Most of the putative target genes of the differentially-expressed miRNAs involved Cancer pathways. Conclusions: Based on literature reviews, many of the miRNAs involved in Cancer pathways were found to be known tumorsuppressive miRNAs, and their target genes were known or putative oncogenes. In conclusion, the expression levels of known tumor-suppressive miRNAs increased at 7 and 18 months, in the PSEN dKO mouse model of AD, supporting the negative correlation between AD and cancer.