• The Korea Journal of Herbology
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• v.34 no.3
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• pp.31-36
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• 2019

Objectives : In this animal study, we performed the single oral dose toxicity test of Standardized Cornus officinalis Sieb. et Zucc. and Psoralea corylifolia L. 30% ethanol extract (SCP) in Sprague-Dawley (SD) rats owing to aims for verifying approximate lethal dose (ALD). Methods : According to OECD guidelines for the testing of chemicals section 4 health effects test No. 420 acute oral toxicity study - fixed dose procedure (17 December 2001), single oral dose toxicity test was performed. Animals were divided into two groups: Group 1, vehicle-treated rats (Control); Group 2, SCP 5000 mg/kg treated rats. SCP is composed of two medicinal herbs: Cornus officinalis Sieb. et Zucc. (650 g) and Psoralea corylifolia L. (350 g) in 30% ethanol. SCP was once orally administered to female and male SD rats at dose levels of 5000 mg/kg. Animals were monitored on the mortality, clinical signs, body weight changes and necropsy findings for 14 days. Results : After single oral treatment of SCP, we could not find any mortality up to 5000 mg/kg. Compared with the control group, there were also no significant differences in clinical sign, weight change, weight gain and gross abnormalities in SCP 5000 mg/kg-treated group. Conclusions : Taken together, these results suggest that the ALD of SCP in both female and male SD rats were considered as over 5000 mg/kg. Results from this study provide scientific evidence for the safety of SCP.

• The Korean Journal of Pesticide Science
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• v.12 no.3
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• pp.229-235
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• 2008

Lack of honey bee toxicity data for most pesticide products used for strawberry restricts to predict the adverse effects to foraging honey bee after treatment of pesticide in plastic house. This study was conducted to evaluate the actual risk of worker honey bees (Apis mellifera L.) through acute contact toxicity test, acute oral toxicity test and toxicity of residues on foliage test with 21 pesticide products. The mortality of honeybee sprayed with 6 pesticides including dichlofluanid WP showed significantly different from control at recommended application rate in acute contact toxicity test at 24 hours after treatment. Fenpropathrin EC and milbemectin EC treatment groups showed more than 25% mortalities at recommended application rate in acute oral toxicity test. In toxicity of residues on foliage test, only fenpropathrin EC treatment group showed more than 25% mortalities at 10 days after treatment at recommended application rate. It was concluded that the most toxic route to exposure for honey bee is direct contact exposure to sprayed pesticides. Safety interval for honey bee was established by concerning the results of these tests.

• The Korea Journal of Herbology
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• v.22 no.2
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• pp.13-16
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• 2007

Objectives : Palmul-tang(Bawu-tang) has been traditionally prescribed a medicine as a restorative. Methods : In this study, we investigated the acute toxicity about water-extracted PalMul-tang(Bawu-tang). Twenty-five mice completed 14 days of oral Palmul-tang(Bawu-tang) at the respective doses of 0(control group), 2560, 3200, 4000 and 5000mg/kg. Results : We observed survival rates, general toxicity, change of body weight, and autopsy. Conclusions : To be confirmed the data for the toxicity and safety problems of oriental medicine prescription. Compared with the control group, we could not find any toxic alteration in all treated groups (2560, 3200, 4000 and 5000mg/kg). In conclusion, LD50 of Palmul-tang(Bawu-tang) was over 5000mg/kg and it is very safe to ICR mice.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.5
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• pp.1192-1195
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• 2008

Sipjeondaebo-tang has been traditionally prescribed a medicine as a restorative. In this study, we investigated the acute toxicity about water-extracted Sipjeondaebo-tang. Thirty rats completed 14 days of oral Sipjeondaebo-tang at the respective doses of 0(control group), 2000 and 5000 mg/kg. We observed survival rates, general toxicity, change of body weight and autopsy. To be confirmed the data for the toxicity and safety problems of oriental medicine prescription. Compared with the control group, we could not find any toxic alteration in all treated groups (2000 and 5000 mg/kg). LD50 of Sipjeondaebo-tang was over 5000 mg/kg and it is very safe to SD rats.

• Toxicological Research
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• v.14 no.2
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• pp.227-235
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• 1998

KH-502 (Flupyrazofos), a new organophosphorus insecticide synthesized by Korea Re-search Institute of Chemical Technology, was found to be effective against diamond-back moth(Plutella xylostella). This study was carried out to determine the acute toxicity of KH-502 in Sprague-Dawley rats and ICR mice. The test article was orally or dermally administered to the animals. Death, tremors, salivation, lacrimation, abnormal gait and corneal opacity were observed. Decrease in body weight gain was observed in all treatment groups. At necropsy, dark red coloration of lung, enlargement of adrenal glands and atrophy of spleen were observed. The oral $LD_{50}$ value was 372 mg/kg in male rats, 605 mg/kg in female rats, 186 mg/kg in male mice, and 115 mg/kg in female mice. And the dermal $LD_{50}$ was 4086 mg/kg in male and 3881 mg/kg in female rats.

• Journal of Food Hygiene and Safety
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• v.9 no.3
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• pp.111-116
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• 1994

The acute toxicity of orally administered Yan-Sheng health liquid (YSHL), water extracts from twelve Chinese drugs (Cervi Cornu, Lonicerae Flos, Foeniculi Fructus, Glycyrrhizae Radix, Liriopis Tuber, Raphani Semen, Bombyx, Ginseng Radix alba, Cinnamomi loureirii Cortex, Epimedii Herba, Zingiberis Rhizoma, Lycii Fructus) was evaluated in male and female Sprague-Dawley rats and ICR mice. Rats and mice aging 5 weeks were gavaged with 0, 2.0, 3.0, 4.4, 6.7, 10.0, 66.7, or 100.0 ml/kg of YSHL. No animal died by oral treatment and no toxic symptom was observed in the treated animals during 5 days. The body weight of the treated animals was not significantly different from the controls. The results of macroscopic examination on the organs of the treated animals revealed no abnormal findings. Therefore, it was concluded that YSHL was practically non-toxic when it was orally administered to rats and mice, and its LD50 was suspected to be greater than 100 ml/kg in rats and mice.

• Korean Journal of Pharmacognosy
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• v.24 no.3
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• pp.258-262
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• 1993

The plant of Pedicularis resupinata var. oppositifolia(Scrophulariaceae) is described to be used for treatment of rheumatoid arthritis, malignant abscess (tumor), urolith and diuretics in oriental medicinal books. This study is concerned with some general pharmacological activities. It was observed that the methanol extract of the aerial part of this plant showed some effects on acute toxicity, carrageenin induced edema in rat paw, bile juice secretion and antibacterial effect. From this result it was obtained that the methanol extract revealed the antiinflammatory activity and bile juice secretion increasing effect. Thus, it was fractionated and buthanol fraction has obvious inhibition effect on edema and bile juice secretion increasing effect. The total extract showed low acute toxicity i.e., the minimum lethal dose was more than 8000mg/kg in oral administration in mice.

• The Korea Journal of Herbology
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• v.26 no.2
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• pp.39-43
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• 2011

Objectives : This study was conducted to evaluate the acute toxicity and safety of Ssanghwa-tang (Shuanhetang in Chinese, Sou-wa-to in Japanese) in Crl : CD Sprague-Dawley (SD) rat though the current regulatory guideline. Methods : In this study, 10 rats of each sex were randomly assigned to two groups of 5 rats each and were administrated singly by gavage at dose levels of 0 and 2000 mg/kg/day of ssanghwa-tang water extract (SHT). After single administration of SHT, mortalities, clinical signs, body weight changes, gross findings were observed for the 15-day period. Results : Acute toxicity tests revealed that a single oral administration of SHT at dose levels of 2000 mg/kg did not affect clinical signs, body weight, and gross findings, evaluating the safety of SHT. The SHT treatment did not result in any toxicologically significant changes in mortality, clinical signs, body weight changes. Conclusions : These results showed that the single oral administration of SHT did not cause any toxic effect at the dose levels of 2000 mg/kg/day in rats. In conclusion, the median lethal dose (LD50) of SHT was considered to be over 2000 mg/kg/day body for both sexes.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.1
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• pp.29-34
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• 2015

The objective of this study was to obtain data on the safety-in-use of nano calcium as a dietary supplement by assessing its acute and subacute oral toxicities in female and male Sprague-Dawley rats. A single oral dose of 5,000 mg/kg of nano calcium did not result in mortality or significant changes in the general behavior and gross appearance of the internal organs of rats. For subacute toxicity study, nano calcium was administered orally at a dose of 1,000 mg/kg daily for 14 days. There were no significant differences in organ weights between control and treated groups of both sexes. Hematological analysis and blood chemistry revealed no toxic effects of nano calcium. Pathologically, neither gross abnormalities nor histopathological changes were observed. These results show that nano calcium possesses very low toxicity as indicated in a rat model.

• Korean Journal of Pharmacognosy
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• v.42 no.3
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• pp.265-270
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• 2011

Acute toxicity of a combined preparation of the standardized extracts Scutellaria baicalensis GEORGI and Salvia miltiorrhiza BUNGE in a ratio of 3:1 was examined in male and female ICR mice. Mice were treated with the test substance intragastrically at a dose of 0 mg/kg, 5 mg/kg, 50 mg/kg, 500 mg/kg or 2,000 mg/kg and observed for two weeks. No death or abnormal clinical sign was shown during the observation period. Also there were no difference in net body weight gain, organ weight, and gross pathological findings at the terminal sacrifice. The results suggested that acute oral toxicity of a combined preparation of the standardized extracts is very low at the conditions employed in this study.