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http://dx.doi.org/10.6116/kjh.2019.34.3.31.

Single Oral Dose Toxicity Test of Standardized Cornus officinalis and Psoralea corylifolia L. Extracts in Sprague-Dawley Rat  

Jin, Bo-Ram (Department of Pharmacology, College of Korean Medicine, Sangji University)
Kang, Hyun-Ae (Department of Pharmacology, College of Korean Medicine, Sangji University)
Cominguez, Divina C. (Department of Pharmacology, College of Korean Medicine, Sangji University)
Kim, Myoung-Seok (Central Research Institue of Hawon Pharmaceutical)
Lee, Kwang-Ho (Central Research Institue of Hawon Pharmaceutical)
Yoon, Il-Joo (Central Research Institue of Hawon Pharmaceutical)
An, Hyo-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University)
Publication Information
The Korea Journal of Herbology / v.34, no.3, 2019 , pp. 31-36 More about this Journal
Abstract
Objectives : In this animal study, we performed the single oral dose toxicity test of Standardized Cornus officinalis Sieb. et Zucc. and Psoralea corylifolia L. 30% ethanol extract (SCP) in Sprague-Dawley (SD) rats owing to aims for verifying approximate lethal dose (ALD). Methods : According to OECD guidelines for the testing of chemicals section 4 health effects test No. 420 acute oral toxicity study - fixed dose procedure (17 December 2001), single oral dose toxicity test was performed. Animals were divided into two groups: Group 1, vehicle-treated rats (Control); Group 2, SCP 5000 mg/kg treated rats. SCP is composed of two medicinal herbs: Cornus officinalis Sieb. et Zucc. (650 g) and Psoralea corylifolia L. (350 g) in 30% ethanol. SCP was once orally administered to female and male SD rats at dose levels of 5000 mg/kg. Animals were monitored on the mortality, clinical signs, body weight changes and necropsy findings for 14 days. Results : After single oral treatment of SCP, we could not find any mortality up to 5000 mg/kg. Compared with the control group, there were also no significant differences in clinical sign, weight change, weight gain and gross abnormalities in SCP 5000 mg/kg-treated group. Conclusions : Taken together, these results suggest that the ALD of SCP in both female and male SD rats were considered as over 5000 mg/kg. Results from this study provide scientific evidence for the safety of SCP.
Keywords
Cornus officinalis; Psoralea corylifolia L.; single oral dose toxicity; SD rats; Single Oral Dose Toxicity Test;
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