• 생약학회지
• /
• 제42권2호
• /
• pp.155-160
• /
• 2011

In this study, we investigated on antioxidative activity, antibacterial activity against pathogens of acne and tyrosinase inhibitory activity in 75% EtOH extract and its fractions from bark of Prunus sargen tii. The total polyphenol and flavonoidcontent of the EtOAc fraction were found to be 378.0 mg/g and 67.5 mg/g as the highest content. In the measurement of DPPH radical scavenging ability, EtOAc and BuOH fraction were exhibited stronger scavenging ability than the other fractions and 75% EtOH extract. In antibacterial activity by disc diffusion assay against pathogen of acne, antibacterial activity of the EtOAc fraction and 75% EtOH extract was stronger than the other fractions. Especially, the EtOAc fraction was the highest effective fraction in the antibacterial activity. Moreover, tyrosinase inhibitory activity of EtOAc fraction, BuOH fraction and 75% EtOH extract was higher than the other fractions. In particular, tyrosinase inhibitory activity of the EtOAc fraction showed higher activity than ascorbic acid used as positive control.

• 한국보건간호학회지
• /
• 제14권2호
• /
• pp.304-317
• /
• 2000

The purpose of this study is to identify the effect of the voluntary program experience with curriculum on self-esteem and depression in nursing college students. The research design utilized in this study was one group pre test-post test design. The data were gathered two times with questionnaire. First data were gathered before voluntary activity. And second data were gathered after instruction and five times voluntary activity. The data were analized by frequency, paired t-test. t-test. and ANOVA using the SAS program. The results were as follows 1. After the activity the scores of self-esteem were significantly higher than before the activity. 2. After the activity the scores of depression were significantly lower than before the activity. 3. The relationship between self-esteem and depression were negatives in both before and after the activity. 4. Before voluntary activity the scores of self-esteem in the class choosing the Culture Exploration were significantly high. but after the activity the scores of self-esteem were not significantly different. 5. As opinion on attendance of lecture class of voluntary activity program. before voluntary activity the scores of depression were not significantly different. but after the activity the scores of depression were significantly different.

• 수산해양교육연구
• /
• 제21권3호
• /
• pp.347-360
• /
• 2009

The purpose of this study was to investigate the characteristics of a teacher's questions in each context of musical activity by analyzing those questions in kindergarten music activity qualitatively. The participant in research was a teacher in charge of the class of children aged 7. Analysis was carried out on the data collected for 3 months through participation observance note, recording material, interview material and weekly plan. As a result, the teacher's questions were leaned upon the convergent questions to confirm the reflection of information and the understanding of function in singing and performing activity. On the other hand, the teacher's questions were leaned upon the diffusive questions for the thought of integration of information in composition activity and upon the diffusive questions for representing feeling or image with no relation to musical meaning in appreciation activity. However, the teacher's questions for control were conducted together with one of the questions, the question mentioned ahead. The tacit control question in singing and performing activity and the explicit control question in composition activity were conducted together, and in appreciation activity, the simple questions to lead the class, though not with the purpose to control attention, was conducted together.

• 대한화장품학회지
• /
• 제26권1호
• /
• pp.199-212
• /
• 2000

A series of novel ascorbic acid derivatives, polyethoxylated ascorbic acid (PEAA) were synthesized by coupling ascorbic acid with polyethylene glycol (PEG) of two molecular weights (MW: 350 and 550) at the C-2 or C-3 hydroxyl group (2PEAA350, 3PEAA350, 2PEAA550, 3PEAA550) to increase the stability and retain the activity, as a skin whitening agent. Their stability, scavenging activity against free radical, inhibitory activity against tyroxinase and inhibitory activity of melanin synthesis in Bl6 melanoma cell of PEAAs were evaluated in viかo and compared with those of ascorbic acid and 3-O-ethyl ascorbic acid (3OEAA), a Con stable vitamin C derivative. Among PEAAs, 2PEAA350 and 2PEAA550 tad high scaveniging activity against See radical, inhibitory activity against tyrosinase and inhibitory activity if melanogenesis but low stability, 3PEAA350 had high stability and moderate scavenging activity against free radical, infibitory activity against tyrosinase and inhibitory activity of melanogenesis. The stability, scavenging activity against free radical and inhibitory activity of melanogenesis of 3PEAA350 were higher than those of 30EAA. The most stable 3PEAA350 among PEAAs was nontoxic in various toxicological tests. These results suggest that PEAA would be a good whitening agent far enhancing stability and bioavailability.

• Child Health Nursing Research
• /
• 제25권3호
• /
• pp.262-272
• /
• 2019

Purpose: We aimed to identify factors influencing physical activity in adolescents with complex congenital heart disease. Methods: We recruited 92 adolescents with complex congenital heart disease from a tertiary medical center in Seoul, measured their levels of physical activity, and identified factors that influenced their physical activity levels using the Global Physical Activity Questionnaire, the New York Heart Association classification, congenital heart disease complexity, the Self-Efficacy Scale, and the Parental Bonding Instrument scale. Stepwise multiple linear regression was used to determine factors influencing physical activity. Results: Total physical activity was higher in males than in females (t=4.46, p<.001). Adolescents who participated in school physical education classes engaged in more physical activity than those who did not (t=6.77, p<.001). Higher self-efficacy (${\beta}=.41$, p<.001), male gender (${\beta}=.37$, p<.001) and participation in school physical education classes (${\beta}=.19$, p=.042) were associated with a higher likelihood of engagement in physical activity. Conclusion: It is necessary to develop nursing interventions that enhance self-efficacy in order to promote physical activity in adolescents with complex congenital heart disease. Physical activity should also be promoted in an individualized manner, taking into account gender, disease severity, and parental attitude.

• 한국조리학회지
• /
• 제23권1호
• /
• pp.95-102
• /
• 2017

This study was activity of ${\alpha}-amylase$, glucoamylase of liquid Nuruk prepared using liquid Nuruk (NK) and Aspergillus kawachii (AK), Aspergillus niger (AN), Aspergillus oryzae (AO), Monascus kaoliang (MK). To investigate the relationship between the enzymatic activity and the total sugar content of liquid Nuruk depending on the types of Nuruk molds and the degree of rice-polishing. The activity of ${\alpha}-amylase$ depending on the types of Nuruk molds was shown to be 8.82, 8.72 units/mL in AN and AK treatments in brown rice liquid Nuruk at 24 hours after incubation, as the degree of rice-polishing increased, the activity of ${\alpha}-amylase$ was significantly lower (p<0.05). When brown rice was incubated in AN, it showed 8.83 units/mL at 48 hours after incubation, which was the highest activity, but there was no significantly difference (p<0.05), as the degree of rice- polishing was higher, the activity of ${\alpha}-amylase$ was lower. The activity of glucoamylase depending on the degree of rice-polishing showed 3,013 units/mL in AO treatment in brown rice liquid Nuruk at 24 hours after incubation, and the enzymatic activity was significantly higher (p<0.05). As the degree of rice-polishing increased, the activity of glucoamylase decreased, so liquid brown rice Nuruk showed the highest enzymatic activity, liquid white rice Nuruk was the lowest enzymatic activity. The highest enzymatic activity appeared in liquid Nuruk with brown rice at 48 hours after incubation. The activity of ${\alpha}-amylase$, glucoamylase showed higher enzymatic productivity as the degree of rice-polishing was lower, and there was an inverse correlation with the total sugar content.

• 한국작물학회지
• /
• 제60권3호
• /
• pp.366-373
• /
• 2015

This study was conducted to clarify the antimicrobial effect, antioxidant and tyrosinase inhibitory activities of the biological composition having the Phytolacca americana, and to enhance the natural materials utilization of foods and cosmetics. The antimicrobial activities of the different parts of P. americana were evaluated using the agar diffusion test. The antimicrobial activity of P. americana was relatively high in Malassezia furfur known as a skin fungi and Vibrio parahaemolyticus compared to Escherichia coli and Staphy-lococcus epidermidis. However, the antimicrobial activity in Vibrio parahaemolyticus did not show at all parts of P. americana. Both the DPPH radical scavenging activity and ABTS radical scavenging activity have been increased with the higher concentration of methanol extract. In particular, leaf extract of P. americana exhibited the highest activity both ABTS radical scavenging activity and DPPH radical scavenging activity. The nitrite scavenging activity was decreased when the pH was changed from pH 1.2 to pH 6.0. The highest nitrite scavenging activity was exhibited from the methanol extract of fruit, followed by root, stem, and leaf at pH 1.2. However, the nitrite scavenging activity at pH of 6.0 was not almost detected. All plant parts of P. americana showed tyrosinase inhibitory activity. The highest activity was found in the stem, and followed by root, leaf, and fruit in order. These tyrosinase inhibitory activity was progressively increased in a concentration-dependent manner. In this experiment on the methanol extracts of different organ from P. americana, we confirmed that the extract of P. americana showed potent tyrosinase inhibitory activity. Taken together, we conjectured that the P. americana had the potent biological activities, therefore this plant having various functional components could be a good material for development into source of natural food additives and cosmetics.

• 가정과삶의질연구
• /
• 제27권5호
• /
• pp.77-90
• /
• 2009

The purpose of this study was to investigate the residents' use and occupancy-behavior in the activity areas of the senior nursing facilities, and to provide basic information to establish the appropriate physical elements for planning the activity areas. For the study, the observations in five facilities were conducted for one day, from 10 a.m. to 4 p.m by four researchers. The results of the study are summarized as follows: First, most of the using behaviors in the activity areas were the doing nothing or sleeping. The meals and program services were provided in only one activity area of the floor and it showed that the unit care system was perfunctorily conducted at those facilities. In the representative activity area, its openness was the main physical element influencing the spatial using frequency, while the accessibility and the openness in the sub-activity area were most important. The seating arrangements having comers were helpful for residents' interactions. Second, while facility programs and meals were provided in the specific activity area, there was no residents' occupancy in other activity areas at the same time. There were interactions including residents' conversations and watching/observations in non-designated activity areas such as the nursing stations and near corridors. But the residents' interactions and self-regulations were blocked by absence of territoriality, monotonous spatial compositions and furniture arrangements, insecurity of residents' privacy, wide or narrow areas, and isolated spatial type. Based on the results at the above, basic guidelines for planning the activity areas of senior nursing facilities can be proposed as follows: First, the isolated type and the sight interception should be avoided in representative activity areas. It should be partitioned with couple of areas through the appropriate furniture arrangements, and be prepared semi-private spaces in non-designated areas such as nursing station for the interactions among the residents and the staff. Second, in activity areas for small group, the isolated type is not also good for the residents' accessibility. The residents' privacy should be confirmed through the various spatial compositions, and enough areas need to be sure for the diverse furniture arrangements.

• 한국조경학회지
• /
• 제29권3호
• /
• pp.29-37
• /
• 2001

The purpose of this study is to determine the suitable illuminance model of an urban neighborhood park. To this end, 1 dependent variable and 11 independent variables were set, and multiple regression analysis was applied to find correlation between variables and the model. The results of this study are as follows; 1) Among 11 independent variables abstracted to study suitable illuminance model of an urban neighborhood park, as a result of analysis on correlation between suitable illuminance of a dependent variable and activity space by using dummy variables, activity type and illuminance by spaces the suitable illuminance required for an urban neighborhood park was much influenced by activity type, and the fact that the activity was not limited to a specific space. 2) As a result of multiple regression analysis, independent variables influencing the suitable illuminance model of an urban neighborhood park were activity space, illuminance by spaces, seated activity, standing activity, and sporting activity. And, for relative contribution of independent variables to suitable illuminance, activity with sporting showed an importance 22 times higher than seated activity. When the central square(Sp_1) of activity spaces was set to reference category using dummy variables, it showed a contribution 52 times higher than sorting space(Sp_7) and the central square as the most important variable. 3) It was analyzed that suitable illuminance of an urban neighborhood part was most influenced by sporting activity but the relative contribution of a sporting space where activity with sporting occurs was least in view of the function of the space. Very high illuminance is required to accept high activity, and if illuminance at a certain space becomes too high, it may influence the illuminance of other spaces, and may consequently have a negative effect on the suitable illuminance of an urban neighborhood park. The results of this study were mainly for teenagers and young adults in their twenties, so further concrete studies which consider gender and a wider age range are needed in the future. Based on subsequent study, suitable illuminance by spaces shall be suggested.

• Journal of Microbiology
• /
• 제41권4호
• /
• pp.289-294
• /
• 2003

Gelatinase is a proteolytic enzyme that hydrolyzes gelatin. Gelatinolytic activity was detected from culture broths of Streptomyces griseus IFO13350 and HH1 by paper disc assays on 0.5% agar plates containing 1% gelatin. The concentrated extracellular protein from the S. griseus was analyzed by SDS polyacrylamide gel, and two proteins, with molecular weights of 30 and 28 kDa, respectively, were identified to have gelatinase activity by gelatin zymography. The protein with a molecular weight of 28 kDa was confirmed to be S. griseus trypsin (SGT). The effects of metal ions and metal chelators on the protease activity of the SGT were studied. Of the metal ions tested, only manganese was found to enhance the protease activity, 2.6 times, however, $Co^{2+},\;Cu^{2+},\;and\;Zn^{2+}$, and metal chelators, such as EDTA and EGTA, inhibited the SGT activity. When the protease activity of the SGT was measured at various pHs, in the presence of 5 mM $MnCl_2$, its highest activity was at pH 11.0, whereas only 60% of the maximum activity was observed between pHs 4.0 and pH 6.0, and almost 80% activity between pHs 7.0 to pH 10.0. The protease activity was measured at various temperatures in the presence of 5 mM $MnCl_2$. The SGT was found to be stable up to $60^{\circ}C$ for 30 min, while only 16% of the enzyme activity remained at $60^{\circ}C$, and at $80^{\circ}C$ almost all the activity was lost. The optimal temperature for the protease activity was $50^{\circ}C$.