• Journal of Microbiology and Biotechnology
• /
• 제18권10호
• /
• pp.1672-1677
• /
• 2008

IscR (iron-sulfur cluster regulator) has been reported to be a repressor of the iscRSUA operon, and in vitro transcription reactions have revealed that IscR has a repressive effect on the iscR promoter in the case of [$Fe_{2}S_{2}$] cluster loading. In the present study, the iscR gene from A. ferrooxidans ATCC 23270 was cloned and successfully expressed in Escherichia coli, and then purified by one-step affinity chromatography to homogeneity. The molecular mass of the IscR was 18 kDa by SDS-PAGE. The optical and EPR spectra results for the recombinant IscR confirmed that an iron-sulfur cluster was correctly inserted into the active site of the protein. However, no [$Fe_{2}S_{2}$] cluster was assembled in apoIscR with ferrous iron and sulfide in vitro. Therefore, the [$Fe_{2}S_{2}$] cluster assembly in IscR in vivo would appear to require scaffold proteins and follow the Isc "AUS" pathway.

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2005년도 추계종합학술대회
• /
• pp.1039-1042
• /
• 2005

Recently, a considerable number of studies have been made on the $RFID^{[1-6]}$ systems. RFID is a technique of identifying an object using radio frequency transmission. The technology can be used to identify, track, sort or detect a wide variety of objects. The RFID system is composed of two main elements: a reader and a tag. Tags can either be active (powered by battery) or passive (powered by the reader field). The passive tags communicate back to the reader with a technique called 'backscatter'. RFID technology can be applied to the supply chain, security, logistics industry and etc. Especially, UHF RFID is worth noticing because of its relatively long identification range and commercial UHF RFID systems are under development. In this paper, we designed and implemented a UHF RFID reader firmware for ISO/IEC 18000-6 Type B specification.

• Journal of Applied Biological Chemistry
• /
• 제51권3호
• /
• pp.95-101
• /
• 2008

The gene encoding a putative esterase of Xanthomonas oryzae pv. oryzae was cloned using PCR technique. The gene was expressed with His6 tag in E. coli. One-step purification of the recombinant esterase with Ni-NTA resin resulted in one band by SDS-PAGE analysis. The purified enzyme showed a molecular weight of 30 kDa, as expected, therefore the enzyme was a mononer. The enzyme was the most active toward p-nitrophenyl (p-NP) acetate and p-NP-butyrate to a lesser extent. However, the enzyme could not hydrolyze p-NP-myristate, palmitate, and stearate. Therefore, the enzyme is considered as an esterase, very different from lipase. The purified esterase had optimal pH at around 8.0 and was stable in a broad range of pH values. The optimal temperature ranged from 30 to $40^{\circ}C$, and the residual activity observed after heat treatment at $55^{\circ}C$ for 20 min was 72 % of the initial activity. The activity was inhibited by the presence of copper and cobalt ions.

• BMB Reports
• /
• 제35권5호
• /
• pp.508-512
• /
• 2002

Phospholipase C-gamma-2 ($PLC{\gamma}2$) activation is a key signaling event for many cell functions. In order to delineate the pathways that lead to $PLC{\gamma}2$ activation, we devised a quick method for obtaining sufficient $PLC{\gamma}2$. We obtained the full-length cDNA for human $PLC{\gamma}2$ and expressed it in E. coli using the expression vector pT5T. To enhance the protein expression, tandem AGG-AGG arginine codons at the amino acid positions 1204-1205 were replaced by CGG-CGG arginine codons. The protein expression was detected in a Western blot analysis by both anti-$PLC{\gamma}2$ antibodies and the antibodies that are raised against the tripeptide epitope (Glu-Glu-Phe) tag that are genetically-engineered to its carboxyl terminal. Crude lysates that were prepared from bacteria that express $PLC{\gamma}2$ were found to catalyze the hydrolysis of phosphatidylinositol 4,5 bisphosphate. Similar to previous reports on $PLC{\gamma}2$ that is isolated from mammalian tissue, the recombinant enzyme was $Ca^{2+}$ dependent with optimal activity at 1-10 uM $Ca^{2+}$.

• Journal of Microbiology and Biotechnology
• /
• 제20권4호
• /
• pp.727-731
• /
• 2010

The gene APE0743 encoding the superoxide dismutase (ApSOD) of a hyperthermophilic archaeon Aeropyrum pernix K1 was cloned and overexpressed as a GST fusion protein at a high level in Escherichia coli. The expressed protein was simply purified by the process of glutathione affinity chromatography and thrombin treatment. The ApSOD was a homodimer of 25 kDa subunits and a cambialistic SOD, which was active with either Fe(II) or Mn(II) as a cofactor. The ApSOD was highly stable against high temperature. This thermostable ApSOD is expected to be applicable as a useful biocatalyst for medicine and bioindustrial processes.

• Journal of Microbiology and Biotechnology
• /
• 제24권2호
• /
• pp.236-244
• /
• 2014

The gene encoding ${\alpha}-\small{L}$-arabinofuranosidase (AFase) from Paenibacillus sp. DG-22 was cloned, sequenced, and expressed in Escherichia coli. The AFase gene (abfA) comprises a 1,509 bp open reading frame encoding 502 amino acids with a molecular mass of 56,520 daltons. The deduced amino acid sequence of the gene shows that AbfA is an enzyme consisting of only a catalytic domain, and that the enzyme has significant similarity to AFases classified into the family 51 of the glycosyl hydrolases. abfA was subcloned into the pQE60 expression vector to fuse it with a six-histidine tag and the recombinant AFase (rAbfA) was purified to homogeneity. The specific activity of the recombinant enzyme was 96.7 U/mg protein. Determination of the apparent molecular mass by gel-filtration chromatography indicated that AbfA has a tetrameric structure. The optimal pH and temperature of the enzyme were 6.0 and $60^{\circ}C$, respectively. The enzyme activity was completely inhibited by 1 mM $HgCl_2$. rAbfA was active only towards p-nitrophephenyl ${\alpha}-\small{L}$-arabinofuranoside and exhibited $K_m$ and $V_{max}$ values of 3.5 mM and 306.1 U/mg, respectively. rAbfA showed a synergistic effect in combination with endoxylanase on the degradation of oat spelt xylan and wheat arabinoxylan.

• 한국통신학회논문지
• /
• 제31권1A호
• /
• pp.58-64
• /
• 2006

900 MHz 대역의 ISO-18000-6B형 표준의 수동형 RFID 리더용 트랜시버를 개발하였다. 송신부의 ASK 변조회로는 GaAs SPST 스위치를 이용하여 고속 저전력 변조 회로로 구성하였으며, 수신부에서는 이중 평형 믹서와 비교기를 이용하여 복조회로를 구성하였다. LO 신호에 대한 우수 고조파 성분들을 억압하고 수신기의 선형성을 향상시키기 위하여 연산 증폭기를 이용한 복조회로와 전압 플로워 및 비교기를 사용하여 회로의 복잡성을 개선하였다. 개발된 트랜시버는 $900{\sim}916\;MHz$ 대역에서 6 dBi의 상용 안테나를 사용하여 5 m의 인식 거리를 얻었다.

• 한국축산식품학회지
• /
• 제37권6호
• /
• pp.813-822
• /
• 2017

The aim of this study was to prepare diacylglycerol (DAG) by enzymatic glycerolysis of lard. The effects of reaction parameters such as lipase type, reaction temperature, enzyme amount, substrate molar ratio (lard/glycerol), reaction time, and magnetic stirring speed were investigated. Lipozyme RMIM was found to be a more active biocatalyst than Novozym 435, and the optimal reaction conditions were 14:100 (W/W) of enzyme to lard substrate ratio, 1:1 of lard to glycerol molar ratio, and 500 rpm magnetic stirring speed. The reaction mixture was first incubated at $65^{\circ}C$ for 2 h and then transferred to $45^{\circ}C$ for 8 h. At the optimum reaction conditions, the conversion rate of triacylglycerol (TAG) and the content of DAG in the reaction mixture reached 76.26% and 61.76%, respectively, and the DAG content in purified glycerolized lard was 82.03% by molecular distillation. The distribution of fatty acids and Fourier transform infrared spectra in glycerolized lard samples were similar to those in lard samples. The results revealed that enzymatic glycerolysis and molecular distillation can be used to prepare more highly purified DAG from lard.

• 한국IT서비스학회지
• /
• 제11권sup호
• /
• pp.61-74
• /
• 2012

As the retail industry has been challenged by stiff competition, the retailer becomes more interested in better understanding consumers' in-store behavior to gain and sustain competitive advantage. Consumers' shopping paths provide valuable clues to understanding customers' in-store behavior, which has been a long standing research issue in business. This study is to explore the shopping path patterns in a grocery using RFID technology and clustering method. To this end, we designed the RFID systems, affixing active RFID tags to the bottom of grocery carts. The tag emit signal that is received by receptors installed at various location throughout the store. The RFID systems provide the time and location of the cart while consumers shop around the store. The point of sale data are matched with the cart movement records to provide a complete picture of each shopping path. To find the distinctive patterns of consumers' shopping paths, we proposed the distance-index matrix using dijkstra method and normalization method to conduct the clustering in order to handle the problem in measuring the similarity among shopping paths, which is raised by the spatial nature of consumer movement in a grocery. After analyzing the RFID data obtained in one of the groceries in a major Korean retailer, we could successfully identify several distinctive patterns of shopping paths, which prove to provide the valuable implications for store management.

• 한국정보과학회:학술대회논문집
• /
• 한국정보과학회 2006년도 가을 학술발표논문집 Vol.33 No.2 (D)
• /
• pp.122-127
• /
• 2006

RFID/USN 은 유비쿼터스 사회의 중요한 기반 인프라로서 고려되고 있다. 또한 다양한 연구를 통해 실용적인 접목이 시도 되고 있다. 특히 물류 시스템에서 물류의 입출 재고와 상태 관리를 위한 연구가 진행 중에 있으며 이는 물품 보관 단위인 파렛트(Pallet) 에 RFID 태그를 부착하여 입/출고시의 물품을 식별하고, Zigbee 무선 통신기능을 가진 센서 모듈에 의해 물품의 환경 상태 정보를 파악하는 형태로 이루어진다. 본 논문은 RFID/ USN 기반의 창고 상태 관리 시스템에서 상태정보 습득 과정의 보안적용을 위한 시스템을 구성을 주된 목적으로 한다. 부가적으로 상태정보 습득에 사용되는 이동형 단말장치의 기능에 대해서 제안한다. 창고 내에 배치되는 RFID 와 센서노드간의 적용 관계 또한 정의한다. 결과적으로 본 논문은 의사판단의 주체인 현장 관리자에 적절한 제어 정보의 제공과 센서의 동작을 제어하기 위한 RFID tag 보안 접근, 그리고 센서 노드 자체의 생존성 이라는 세 가지 관점의 절충점을 찾기 위한 시도로서 설계하였다.