Journal of Microbiology and Biotechnology

  • 제20권4호
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  • Pages.727-731
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  • 2010
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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An Efficient Method for the Expression and Reconstitution of Thermostable Mn/Fe Superoxide Dismutase from Aeropyrum pernix K1

  • Lee, Hee-Jin (Department of Chemistry, College of Natural Sciences, Chung-Ang University) ;
  • Kwon, Hye-Won (Department of Chemistry, College of Natural Sciences, Chung-Ang University) ;
  • Koh, Jong-Uk (Department of Chemistry, College of Natural Sciences, Chung-Ang University) ;
  • Lee, Dong-Kuk (Department of Fine Chemistry, Seoul National University of Technology) ;
  • Moon, Ja-Young (Department of Biochemistry, College of Natural Sciences, Changwon National University) ;
  • Kong, Kwang-Hoon (Department of Chemistry, College of Natural Sciences, Chung-Ang University)
  • 투고 : 2009.11.04
  • 심사 : 2009.12.21
  • 발행 : 2010.04.28
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초록

The gene APE0743 encoding the superoxide dismutase (ApSOD) of a hyperthermophilic archaeon Aeropyrum pernix K1 was cloned and overexpressed as a GST fusion protein at a high level in Escherichia coli. The expressed protein was simply purified by the process of glutathione affinity chromatography and thrombin treatment. The ApSOD was a homodimer of 25 kDa subunits and a cambialistic SOD, which was active with either Fe(II) or Mn(II) as a cofactor. The ApSOD was highly stable against high temperature. This thermostable ApSOD is expected to be applicable as a useful biocatalyst for medicine and bioindustrial processes.

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참고문헌

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피인용 문헌

  1. SORGOdb: Superoxide Reductase Gene Ontology curated DataBase vol.11, pp.None, 2010, https://doi.org/10.1186/1471-2180-11-105
  2. Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability vol.278, pp.4, 2010, https://doi.org/10.1111/j.1742-4658.2010.07977.x
  3. Characterization of a Thermophilic l-Rhamnose Isomerase from Caldicellulosiruptor saccharolyticus ATCC 43494 vol.59, pp.16, 2010, https://doi.org/10.1021/jf201428b
  4. Iron-dependent superoxide dismutase from novel thermoacidophilic crenarchaeon Acidilobus saccharovorans: from gene to active enzyme vol.77, pp.12, 2012, https://doi.org/10.1134/s0006297912120048
  5. Cloning and characterization of a new manganese superoxide dismutase from deep-sea thermophile Geobacillus sp. EPT3 vol.30, pp.4, 2014, https://doi.org/10.1007/s11274-013-1536-5
  6. Cloning and characterization of a differentially expressed mitochondrial manganese superoxide dismutase gene from Pleurotus ostreatus vol.65, pp.3, 2010, https://doi.org/10.1007/s13213-014-0999-4
  7. Cloning and characterization of iron‐superoxide dismutase in Antarctic yeast strain Rhodotorula mucilaginosa AN5 vol.57, pp.8, 2010, https://doi.org/10.1002/jobm.201700165