• BMB Reports
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• v.29 no.4
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• pp.386-392
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• 1996

A Saccharomyces cerevisiae Protein Tyrosine Phosphatase, PTP1, was expressed from an Escherichia coli expression system and milligram quantities of active PTP1 were purified chromatographically. The substrate specificity of the recombinant PTP1 was probed using synthetic phosphotyrosine-containing peptides corresponding to the regulatory phosphorylation sites of the yeast MAP kinase homologues $Fus3_{176-186}$, $Kss1_{179-189}$, and $Hog1_{170-180}$. Peptide sequences derived from the MAP kinase homologues were chosen arbitrarily as starting points for sequence variation studies even though they are not likely to be candidates for physiological substrates of PTP1. Phosphotyrosyl-$Hog1_{170-180}$ peptide showed a $K_M$ value of 877 ${\mu}M$ and phosphorylated $Kss1_{179-189}$ and $Fus3_{176-186}$ peptides showed lower $K_M$ values of 74 ${\mu}M$ and 51 ${\mu}M$ each. To study the effect of sequence variations of the peptide, amino acids of the undecapeptide $Hog1_{170-180}$ (DPQMTGpYVSTR) were sequentially substituted by an alanine residue. More extensive variations of each amino acid revealed positional importance of each amino acid residue. Based on these results, we derived a peptide sequence (DADEpYDA) that is recognized by PTP1 with an affinity ($K_M$ is 4 ${\mu}M$) significantly higher than that of the peptides derived from the phosphorylation sites of Fus3, Kss1, and Hog1.

• Journal of KIISE:Software and Applications
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• v.34 no.10
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• pp.937-944
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• 2007

In this paper, we propose multiple human tracking with an moving stereo camera. The tracking process is based on mean shift algorithm which is using color information of the target. Color based tracking approach is invariant to translation and rotation of the target but, it has several problems. Because of mean shift uses color distribution, it is sensitive to color distribution of background and targets. In order to solve this problem, we combine color and depth information of target. Also, we build human body part model to handle occlusions and we have created adaptive box scale. As a result, the proposed method is simple and efficient to track multiple humans in real time.

• The Bulletin of The Korean Astronomical Society
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• v.36 no.2
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• pp.85.2-85.2
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• 2011

A new infrared spectro-polarimeter was installed in 2008 onto the Solar Flare Telescope of NAOJ in the Mitaka headquarters. The Solar Flare Telescope had been operated previously as a filter-based magnetograph and obtained vector magnetograms of active regions with the Fe I 630.3nm line during 1992 - 2005. The aim of this new instrument is to measure the distribution of magnetic helicity over the whole Sun and for an extended period with high magnetic sensitivity in the infrared wavelengths. This spectro-polarimter is able to obtain polarizations in both photospheric and chromospheric layers. In order to take full Stokes profiles, we observe Fe I 1564.8 nm and He I 1083.0 nm lines (with the neighboring photospheric Si line) for the photospheric and chromospheric magnetic field vectors, respectively. The infrared detector of this instrument is a $640{\times}512$-pixel InGaAs camera produced by a Belgian company Xenics. The frame rate of the camera is 90 frames/sec. The 640-pixel row of this camera is set along the spectrograph slit of the polarimeter. Since the slit only covers the solar hemisphere, a full disk map is obtained by raster scanning the solar disk twice. A magnetic map is made of about $1200{\times}1200$ pixels with a pixel size of 1.8 arcsec. It generally takes 1.5 hours to scan the whole Sun. Although some issues on the instrument calibration still remain, a few maps of the whole Sun at the two wavelengths are now taken daily. In this presentation, we will introduce the instrument and present some observational results.

• The Journal of the Korea Contents Association
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• v.21 no.10
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• pp.184-193
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• 2021

Interactive documentary refers to a new style of documentary that is created and accepted through active interaction. It is attracting attention as a platform that forms a public sphere and mediates audiences to participate in social change. However, the possibilities was not systematically explored, and there was insufficient consideration on how to realize them. In this paper, discussions on the political aesthetics of Walter Benjamin are examined, and the media characteristics of interactive documentary are analyzed through text mining. Then, by connecting the two to each other, we draw a map of the political and aesthetic possibilities, and based on the map, we analyze the actual works. This study has the value of establishing a theoretical framework for the possibilities of interactive documentaries. In the follow-up study, we will consider the practical strategy of interactive documentary as a transmedia activism and develop a practical analysis and planning methodology.

• Journal of The Geomorphological Association of Korea
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• v.24 no.1
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• pp.77-91
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• 2017

Many debris landforms in the mountains of Korea have formed in the periglacial environment during the last glacial stage when the generation of sediments was active. Because these landforms are generally located on steep slopes and mostly covered by vegetation, however, it is difficult to observe and access them through field investigation. A scientific method is required to reduce the survey range before performing field investigation and to save time and cost. For this purpose, the use of remote sensing and GIS technologies is essential. This study has extracted the potential area of debris landform formation using a fuzzy set model as a mathematical data integration method. The first step was to obtain information about the location of debris landforms and their related factors. This information was verified through field observation and then used to build a database. In the second step, we conducted the fuzzy set modeling to generate a map, which classified the study area based on the possibility of debris formation. We then applied a cross-validation technique in order to evaluate the map. For a quantitative analysis, the calculated potential rate of debris formation was evaluated by plotting SRC(Success Rate Curve) and calculating AUC(Area Under the Curve). The prediction accuracy of the model was found to be 83.1%. We posit that the model is accurate and reliable enough to contribute to efficient field investigation and debris landform management.

• Journal of the Korea Safety Management & Science
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• v.22 no.4
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• pp.9-15
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• 2020

As of 2018, total yield of lettuce and strawberry amounted to 93,543 tons (representing 1.0 percent) and 183,639 tons (2.0 percent), respectively, among total yields worth 9,185,889 tons in South Korea. Lettuce is affected by a combination of numerous elements such as varieties, cultivation methods and pests during each growth phase (Lee et al., 1999). It is mainly cultivated in spring and fall. Especially due to respiration rate after harvest leading to reduced quality and poor storage, maintaining annual supply is unavailable (Jang et al., 2018). With the distribution of new varieties, forcing culture and indoor insulated facilities for plant cultivation during winter, strawberries are produced every year except for late summer and early fall. Due to active respiration, transpiration, soft flesh and high water content, the fruit is vulnerable to go bad and got rotten compared to other fruits. Furthermore, it is difficult to maintain freshness due to the possibility of softening, discoloration and fungi (Lee et al., 2012). In this regard, developing improved storage and package techniques is needed to ensure maintaining quality and safety even just two to three days after harvest. In order to ensure improved quality and safety of strawberries and lettuce after harvest, the present study applied a modified atmosphere packaging (MAP) technology (Mostofi et. al., 2008). Going forward, it compared the quality and safety of the two products while being stored in a way that put them in an MAP-applied container and a plastic container at room temperature and 4 degree Celsius.

• The Journal of Engineering Geology
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• v.31 no.4
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• pp.549-559
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• 2021

The Arctic Circle's huge amounts of fossil fuels and mineral resources are being developed and subjected to active construction projects. Global efforts are continuing to actively respond to climate change, but the dependence on fossil fuels remains high. This study reports a preliminary survey conducted in Alberta, Canada, where oil sand resources are actively developed. A land cover map was prepared using satellite imagery to reduce the cost and time of surveying a wide area. Results likely useful to resource development projects such as ground surface temperature and snow cover distribution were derived by using the obtained image classification results. It is expected that the results of the present research and analysis will be used to establish strategies for the successful promotion and operation of projects to develop resources in the Arctic.

• Structural Engineering and Mechanics
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• v.78 no.3
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• pp.351-368
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• 2021

This paper, by applying a reliability-based framework, develops seismic vulnerability macrozonation maps for Tehran, the capital and one of the most earthquake-vulnerable city of Iran. Seismic performance assessment of 3-, 4- and 5-story steel moment resisting frames (SMRFs), designed according to ASCE/SEI 41-17 and Iranian Code of Practice for Seismic Resistant Design of Buildings (2800 Standard), is investigated in terms of overall maximum inter-story drift ratio (MIDR) and unit repair cost ratio which is hereafter known as "damage ratio". To this end, Tehran city is first meshed into a network of 66 points to numerically locate low- to mid-rise SMRFs. Active faults around Tehran are next modeled explicitly. Two different combination of faults, based on available seismological data, are then developed to explore the impact of choosing a proper seismic scenario. In addition, soil effect is exclusively addressed. After building analytical models, reliability methods in combination with structure-specific probabilistic models are applied to predict demand and damage ratio of structures in a cost-effective paradigm. Due to capability of proposed methodology incorporating both aleatory and epistemic uncertainties explicitly, this framework which is centered on the regional demand and damage ratio estimation via structure-specific characteristics can efficiently pave the way for decision makers to find the most vulnerable area in a regional scale. This technical basis can also be adapted to any other structures which the demand and/or damage ratio prediction models are developed.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.23 no.2
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• pp.1-6
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• 2023

The purpose of this study is to research and develop so that users who are gradually progressing in the popularization of smartphones and the calculation of agricultural quality can use more active and flexible applications than existing application fields. People use event management applications to remember what they need to do, and maps applications to get to their appointments on time. You will need to build a glue-delivered application that leverages the Maps API to be able to recommend the glove's path for events so that the user can use the application temporarily. By comparing and analyzing currently used calendar, map, and schedule applications, several Open Maps APIs were compared to supplement the weaknesses and develop applications that converge the strengths. The results of application development by applying the optimal algorithm for recommending traffic routes according to time and place for the schedule registered by the user are described.

• Herbal Formula Science
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• v.31 no.4
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• pp.245-252
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• 2023

Objectives : The purpose of this study was to explore the compounds, targets and related diseases of garlic by the approaches of network pharmacology and bioinformatics in traditional chinese medicine. Methods : We investigated components and their target molecules of garlic using SymMap and TCMSP and they were compared with analysis platform. Results : 56 potential compounds were identified in garlic, 26 of which contained target information, and it was found that these 26 compounds and 154 targets interact with each other through a combination of 243 compounds. In addition, Apigenin was linked to the most targeted gene (78) in 26 compounds, followed by Kaempferol (61 genes), Nicotic Acid (14 genes), Geraniol (11 genes), Eee (10 genes), and Sobrol A (9 genes). Among 56 potential compounds, three compounds (Kaempferol, Dipterocarpol, and N-Methyl cytisine) corresponded to the active compound by screening criterion Absorption, Distribution, Metabolism, Excretion (ADME). In addition, 12 compounds in 56 potential compounds were associated with gastrointestinal (GI) motility disorder. Among them, Kaempferol was a compound that met the ADME parameters and the rest were potential compounds that did not meet. Also, Kaempferol was closely related to GI motility disorder, indicating that this Kaempferol could be a candidate for potential medical efficacy. Conclusions : It shows the relationship between the compound of garlic, an herbal supplement, and the biological process associated with GI motility disorder. These results are thought to help develop strategies for treating GI motility disorders.