• 한국의학물리학회지:의학물리
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• 제12권2호
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• pp.155-160
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• 2001

3차원적인 타겟 모양을 축소 제작하여 액체 차폐물 속에 넣고 이를 방사선의 경로상에 장착하여 갠트리가 회전하더라도 제작된 타겟의 원래의 방향이 변하지 않도록 중력방향에 따라 회전하게 함으로써 액체 차페물(수은)의 두께차에 의해 방사선의 강도를 변화시킴은 물론 다엽콜리메터를 쓰지 않고도 갠트리 방향의 변화에 따른 방사선조사야의 모양이 자동적으로 이루어지도록 하는 방법을 개발하였다. 갠트리 0$^{\circ}$, 45$^{\circ}$, 90$^{\circ}$ 에서 5cm의 깊이에 필름을 두고 강도변화를 측정한 결과 갠트리가 회전함에 따라 실제 타겟의 3차원적 모양에 따른 강도 변화가 이루어지고 있음을 볼 수 있었다. 이는 외부에서 어떤 영향을 가하지 않고 갠트리 방향만 회전함에 따른 결과로 아크릴 삽입체의 방향이나 위치가 항상 일정하게 유지되고 있고, 그에 따라 방사선의 강도변화가 자동적으로 수행되고 있음을 보여주었다. 직경 25cm 아크릴 팬텀 내에 필름을 넣고 0$^{\circ}$ 에서부터 45$^{\circ}$ 간격으로 등선량으로 8문 조사를 한 결과 타겟의 isocenter에 선량을 100%로 할 경우 80% 영역이 타겟을 적절히 감싸고 있음을 볼 수 있었다. 차후 최적화된 선량게획 시스템을 이용할 경우 지금의 선량분포 보다 타겟모양에 더욱 정확한 분포를 얻을 수 있을 것으로 생각된다.

• 환경정책연구
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• 제11권4호
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• pp.3-37
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• 2012

우리나라의 국토 및 환경 분야를 다루는 다양한 국가계획에서 생물다양성 보전 및 보호지역 주요 이슈가 어떻게 다뤄지고 있는지를 고찰하고 시사점을 도출하고자 하였다. 생물다양성협약의 "생물다양성 2011~2020 전략목표"와 "보호지역 결정문" 내용을 토대로 주요 이슈를 선정한 후, 주요 이슈별로 우리나라 국토 및 환경 관련 주요 중장기 국가계획 7개의 내용을 정리 분석하였다. 생물다양성 2011~2020 전략목표 5와 관련하여 녹지총량제, 습지총량제 등 거의 모든 국가계획에서 다양한 제도 도입과 활용을 다루고 있으며, 보호지역 확대와 관련된 전략목표 11(2020년까지 육상 육수 생태계 17%, 연안 해양 생태계 10%)의 경우 우리나라는 국토면적 대비 보호지역 15%(2015년), 연안 해양보호구역 13%(2015년, 2020년)로 목표를 제시하고 있다. 보호지역 결정문의 주요 권고사항인 국가별 보호지역 실행프로그램(PoWPA)의 장기 이행계획 수립 개정에 대한 내용은 언급되지 않고 있으나, 관심이 필요한 10가지 보호지역 이슈에 대해서는 각종 국가계획에서 다양하게 다뤄지고 있는 것으로 나타났다. 하지만, 그 내용의 충분성 및 국가 계획별 상호 연계성 등에 있어서는 개선의 여지가 많음을 보여주고 있었다. 특히 "보호지역 관리효과성평가"의 경우 당사국에 2015년까지 국가 보호지역의 60%까지 수행하고 그 결과를 보고하도록 권고하고 있으나, 각종 국가계획에서 이에 대한 직접적 기술은 없는 것으로 나타났다. 고찰내용을 토대로 한 제안사항은 (1) "국토종합계획"에서 자연환경 보전이 국가경쟁력의 주요 요소임을 인지하고 생물다양성 및 보호지역 이슈에 대한 내용 강화, (2) 관련 국가계획들간의 상호 연계성 강화를 통한 효과적인 국가 보호지역 목표 성취를 위해 국가 환경 분야의 최상위 국가계획에서 범부처 차원의 "국가 보호지역시스템 계획" 수립, (3) 국가차원의 "보호지역실행프로그램 중장기 이행계획" 수립, (4) 각종 국가계획에 "생물다양성 전략목표"와 관련된 성과목표 설정과 보호지역결정문에서 권고하고 있는 주요 이슈들 중 관리효과성평가 등 미흡한 분야에 대한 내용 강화이다.

• Asian-Australasian Journal of Animal Sciences
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• 제29권5호
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• pp.731-738
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• 2016

Glucagon-like peptide-2 (GLP-2) is important for intestinal barrier function and regulation of tight junction (TJ) proteins, but the intracellular mechanisms of action remain undefined. The purpose of this research was to determine the protective effect of GLP-2 mediated TJ and transepithelial electrical resistance (TER) in lipopolysaccharide (LPS) stressed IPEC-J2 cells and to test the hypothesis that GLP-2 regulate TJ and TER through the phosphatidylinositol 3-kinase (PI3K)-protein kinase B (Akt)-mammalian target of rapamycin (mTOR) signaling pathway in IPEC-J2 cells. Wortmannin and LY294002 are specific inhibitors of PI3K. The results showed that $100{\mu}g/mL$ LPS stress decreased TER and TJ proteins occludin, claudin-1 and zonula occludens protein 1 (ZO-1) mRNA, proteins expressions (p<0.01) respectively. GLP-2 (100 nmol/L) promote TER and TJ proteins occludin, claudin-1, and zo-1 mRNA, proteins expressions in LPS stressed and normal IPEC-J2 cells (p<0.01) respectively. In normal cells, both wortmannin and LY294002, PI3K inhibitors, prevented the mRNA and protein expressions of Akt and mTOR increase induced by GLP-2 (p<0.01) following with the significant decreasing of occludin, claudin-1, ZO-1 mRNA and proteins expressions and TER (p<0.01). In conclusion, these results indicated that GLP-2 can promote TJ's expression and TER in LPS stressed and normal IPEC-J2 cells and GLP-2 could regulate TJ and TER through the PI3K/Akt/mTOR pathway.

• Molecules and Cells
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• 제37권8호
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• pp.620-627
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• 2014

We have previously shown that microRNAs (miRNAs) miR-760, miR-186, miR-337-3p, and miR-216b stimulate premature senescence through protein kinase CK2 (CK2) downregulation in human colon cancer cells. Here, we examined whether these four miRNAs are involved in the replicative senescence of human lung fibroblast IMR-90 cells. miR-760 and miR-186 were significantly upregulated in replicatively senescent IMR-90 cells, and their joint action with both miR-337-3p and miR-216b was necessary for efficient downregulation of the ${\alpha}$ subunit of CK2 ($CK2{\alpha}$) in IMR-90 cells. A mutation in any of the four miRNA-binding sequences within the $CK2{\alpha}3^{\prime}$-untranslated region (UTR) indicated that all four miRNAs should simultaneously bind to the target sites for $CK2{\alpha}$ downregulation. The four miRNAs increased senescence-associated ${\beta}$-galactosidase (SA-${\beta}$-gal) staining, p53 and $p21^{Cip1/WAF1}$ expression, and reactive oxygen species (ROS) production in proliferating IMR-90 cells. $CK2{\alpha}$ overexpression almost abolished this event. Taken together, the present results suggest that the upregulation of miR-760 and miR-186 is associated with replicative senescence in human lung fibroblast cells, and their cooperative action with miR-337-3p and miR-216b may induce replicative senescence through $CK2{\alpha}$ downregulation-dependent ROS generation.

• 동의생리병리학회지
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• 제34권3호
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• pp.136-141
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• 2020

The purpose of this study was to investigate the action mechanism of the roots of Adenophora triphylla var. japonica extract (ATE) in 3T3-L1 adipocytes. Cell toxicity test by MTT assay and lipid accumulation was performed to evaluate the inhibitory effect on the differentiation of adipocyte from preadipocytes induced by MDI differentiation medium, while adipogenesis related proteins expression level were evaluated by western blotting. As a result, ATE inhibited MDI-induced adipocyte differentiation in 3T3-L1 cells dose-dependently without cytotoxicity. Our results showed that ATE inhibited the phosphorylation of IRS1, thereby decreasing the expression of PI3K110α and reducing the phosphorylation of AKT and mTOR, resulting in attenuated protein expression of C/EBPα, PPARγ, ap2 and FAS in 3T3-L1 cells. These results suggest anti-adipogenic functions for ATE, and identified IRS1 as a novel target for ATE in adipogenesis.

• 국제학술발표논문집
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• The 8th International Conference on Construction Engineering and Project Management
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• pp.313-322
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• 2020

The recognition of the risk hazards is a vital step to effectively prevent accidents on a construction site. The advanced development in computer vision systems and the availability of the large visual database related to construction site made it possible to take quick action in the event of human error and disaster situations that may occur during management supervision. Therefore, it is necessary to analyze the risk factors that need to be managed at the construction site and review appropriate and effective technical methods for each risk factor. This research focuses on analyzing Occupational Safety and Health Agency (OSHA) related to risk zone identification rules that can be adopted by the image recognition technology and classify their risk factors depending on the effective technical method. Therefore, this research developed a pattern-oriented classification of OSHA rules that can employ a large scale of safety hazard recognition. This research uses joint reasoning of risk zone Identification and numeric input by utilizing a stereo camera integrated with an image detection algorithm such as (YOLOv3) and Pyramid Stereo Matching Network (PSMNet). The research result identifies risk zones and raises alarm if a target object enters this zone. It also determines numerical information of a target, which recognizes the length, spacing, and angle of the target. Applying image detection joint logic algorithms might leverage the speed and accuracy of hazard detection due to merging more than one factor to prevent accidents in the job site.

• International Journal of Quality Innovation
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• 제8권3호
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• pp.173-187
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• 2007

Many 6-sigma innovation companies are obtaining ISO9000 series or ISO/TS16949 certifications. However, not many of them have considered the integrated management of ISO/TS16949 at the time of 6-sigma introduction. ISO/TS16949 focuses on the process of an overall company. In particular, APQP (Advanced Product Quality Planning) requires that from the beginning all the planning should have a clear quality planning business process. Each company can decide the best course of action to suit its individual needs. Lean DFSS in 6-sigma offers the concrete development steps of the product development process. And if analyses of Input, Output, Target, Process, KSF, KPI, and FMEA is done in each process and clearly defined in APQP, mutual organic and effective systems can be initially achieved.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.245.3-246
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• 2003

S-Adenosylhomocysteine hydrolase (SAH) catalyzes the hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine and has been an attractive target for the development of broad spectrum antiviral agents. Based on the potent inhibitory activity of neplanocin A against SAH, we have reported the synthesis and novel mechanism of action of fluoro-neplanocin A. (omitted)

• 한국수산과학회지
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• 제28권3호
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• pp.309-315
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• 1995

This paper describes a monitoring system for fish farm formation effect based on personal computer by using an image processing technique. This method is based on image processing technique incorporating concept of window and threshold processing to track the target object and to distinguish it from background. The image processing program runs in the veal time so that all program modules are able to process multi-task. The effectiveness is evaluated through the comparative study on the motion of lantern net for the scallop culturing by wave action in an experimental wave tank.

• Molecules and Cells
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• 제28권4호
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• pp.403-406
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• 2009

Rev is an essential regulatory protein for HIV-1 replication. Rev (11-20) is known as the significant region regarding the function of a nuclear entry inhibitory signal (NIS) of Rev. In this study, anticandidal effects and mechanism of action of Rev (11-20) were investigated. The result exhibited that Rev (11-20) contained candidacidal activities. To understand target site(s) of Rev (11-20), the intracellular localization of the peptide was investigated. The result showed that Rev (11-20) rapidly accumulated in the fungal cell surface. The cell wall regeneration test also indicated that Rev (11-20) exerted its anticandidal activity to fungal plasma membrane rather than cell wall. The fluorescent study using 1,6-diphenyl-1,3,5-hexatriene (DPH) further confirmed the membrane-disruption mechanism(s) of Rev (11-20). The present study suggests that Rev (11-20) possesses significant potential regarding therapeutic agents for treating fungal diseases caused by Candida species in humans.