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http://dx.doi.org/10.1007/s10059-009-0136-6

Candidacidal Effects of Rev (11-20) Derived from HIV-1 Rev Protein  

Lee, Juneyoung (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
Lee, Dong Hwan (Christian Academy in Japan)
Lee, Dong Gun (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
Publication Information
Molecules and Cells / v.28, no.4, 2009 , pp. 403-406 More about this Journal
Abstract
Rev is an essential regulatory protein for HIV-1 replication. Rev (11-20) is known as the significant region regarding the function of a nuclear entry inhibitory signal (NIS) of Rev. In this study, anticandidal effects and mechanism of action of Rev (11-20) were investigated. The result exhibited that Rev (11-20) contained candidacidal activities. To understand target site(s) of Rev (11-20), the intracellular localization of the peptide was investigated. The result showed that Rev (11-20) rapidly accumulated in the fungal cell surface. The cell wall regeneration test also indicated that Rev (11-20) exerted its anticandidal activity to fungal plasma membrane rather than cell wall. The fluorescent study using 1,6-diphenyl-1,3,5-hexatriene (DPH) further confirmed the membrane-disruption mechanism(s) of Rev (11-20). The present study suggests that Rev (11-20) possesses significant potential regarding therapeutic agents for treating fungal diseases caused by Candida species in humans.
Keywords
antifungal peptide; Candida spp.; membrane-disruption mechanism; Rev (11-20);
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