• 한국임상수의학회지
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• 제29권4호
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• pp.352-355
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• 2012

Hypospadias is a rare congenital malformation of the urethra reported in cattle. The urethral lumen of male indigenous Korean calf is open along the ventral aspect of the penis in the perineal region. Renal abscess and renal stone formation causing urinary tact infection has not been reported in hypospadia calves. The objective of this study was investigation for renal abscess and renal stone formation through autopsy. Histopathological examination and laboratory tests were performed. At autopsy, the pustules were formed on the right renal cortex, and the renal medulla abscess were formed on right and left part of the renal pelvis. Histopathological finding, this case was diagnosed as severe acute suppurative and necrotizing pyelonephritis, and severe chronic interstitial nephritis with fibrosis and moderate multifocal acute cystitis with edema. Milky exudate of the kidney has been identified as Actinomyces meyeri using the VITEK-2 system for identification of bacteria, and the stone has been identified as carbonate apatite using FT-IR system for quantification analysis. This case report describe the hypospadias complicated with urinary tract infection due to carbonate apatite stones and Actinomyces meyeri.

• Tuberculosis and Respiratory Diseases
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• 제45권4호
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• pp.870-875
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• 1998

방선균과 아스페르질루스종 각각은 비교적 흔한 질환이나 폐의 동일 병변에서 방선균과 아스페르질루스가 혼합 감염된 예는 보고 된 바가 없는데 최근 객혈로 내원한 62세 여자 환자의 고립성 폐결절에서 방선균파 아스페르질루스의 혼합 감염을 확인하였기에 보고 하는 바이다.

• Pediatric Infection and Vaccine
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• 제28권2호
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• pp.118-123
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• 2021

세균 감염에서 적절한 항생제를 선택하려면 배양검사가 매우 중요하다. 저자들은 복부 연조직 감염의 농양을 혈액배양병을 사용하여 시행한 배양검사에서 Actinomyces radingae와 Clostridium ramosum이 배양된 증례를 경험하여 보고하고자 한다. 이전에 건강하였던 13세 남자 환자가 배꼽주변에 발생한 통증, 발적 및 발열을 주소로 응급실에 내원하였다. 환아는 복부 수술 및 외상의 병력은 없었다. 컴퓨터 단층 촬영에서 배꼽주위에 농양을 동반한 피부 연조직염이 확인되었고 선천성 기형은 없었다. 초음파 유도 흡인을 하여 8.5 mL의 화농성 농양이 흡인되었고, 농양은 혈액배양병을 이용하여 배양하였다. 농양 배양검사에서 A. radingae와 C. ramosum이 확인되었다. 감염증의 원인이 드문 세균일 가능성이 있는 경우 농양 배양을 할 때 일반적인 농양배양의 방법 보다는 혈액배양병을 사용하는 것이 원인균이 분리될 가능성을 높이고 더 빨리 확인할 수 있을 것으로 사료된다.

• 대한치과교정학회지
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• 제30권3호
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• pp.335-342
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• 2000

본 연구의 목적은 chlorhexidine varnish를 고정식 교정장치 장착 환자의 치면에 도포한 후 나타나는 치태내 균주의 변화 양상을 장기간 관찰하는 것이다. 서울대학교병원 치과진료부 교정과에서 고정치 교정장치로 치료하기로 하였던 환자 100명중에서 32주까지 장기간 추적 (follow-up) 되었던 21명을 최종 연구대상으로 선정하였다. 실험군 (12명) 은 chlorhexidine varnish를 1주일 간격으로 4회 도포한 후 고정식 교정장치를 부착하였고, 대조군 (9명) chlorhexidine varnish 처치를 하지 않고 고정식 교정장치를 부착하였다. 그리고 실험군에서는 20주 째에 chlorhexidine varnish를 1회 처치하였다. 두 군에서 고정식 교정장치 부착 직전과 부착 후 4, 8, 20, 32주의 치태내 균주의 변화 양상을 간접면역 형광 현미경법으로 분석하고 SAS 통계프로그램을 사용하여 다음과 같은 결과를 얻었다. 1. Streptococcus mutans는 실험군에서 전 실험기간동안 통계학적으로 유의성 있게 억제되 었고 (p<0.01), 대조군에서 전 실험기간동안 통계학적으로 유의성 있게 증가하였다 (p<0.05). 2. Streptococcus sanguis, Streptococcus mitis, Actinomyces viscosus, Actinomyces naeslundii는 실험군과 대조군에서 통계학적으로 유의성 있는 변화를 보이지 않았다. 따라서 고정식 교정장치를 사용할 환자에서 chlorhexidine varnish를 처치할 경우 치아 우식의 주 원인균인 Streptococcus mutant를 장기간 선택적으로 억제할 수 있을 것으로 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권1호
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• pp.61-65
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• 2005

The objective of this work was to investigate the nutritional requirements for the growth of Actinomyces sp. 9RCC5 isolated from the rumen of a native goat in Korea. The growth of strain 9RCC5 on the basal medium or the medium minus certain ingredients from the basal medium demonstrated that strain 9RCC5 showed absolute requirement of vitamin B complex mixture, while hemin and volatile fatty acids (VFA) were stimulatory to growth to some extent. The 9RCC5 strain grew well with casein hydrolysate as the sole added nitrogen source. However, neither a complex of 18 amino acids nor ammonium sulfate effectively replaced casein hydrolysate. Vitamins such as riboflavin and pantothenate were essential for growth, while thiamin and biotin were stimulatory. With regard to VFA, the growth was stimulated by acetic acid but inhibited by valeric acid. Relatively large quantities of $Na^+$, $K^+$ and $Ca^{2+}$ were absolutely required for growth. Supplementation of clarified rumen fluid to the basal medium in a range of 0-10% (vol/vol) resulted in an increased rate of growth as well as an increased extent of growth.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제30권3호
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• pp.296-301
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• 2008

Actinomyces is a part of the normal oral flora, but under certain circumstances it may become pathogenic. Actinomycosis is a chronic granulomatous infective disease caused by microaerophilic Gram-positive bacteria of the genus actinomyces. It can involve almost any system, but principally affects the head and neck. Because the lesions in the submandibular region and the angle of the jaw give the face a swollen, indurated appearance, actinomycosis of mandible can be easily misdiagnosed in its acute or early state of infection. In these cases the disease usually presented as a swelling suggestive of an abscess or mimicking a neoplasm. The yield from standard cultures was poor and repeated sampling and anaerobic culture may be needed to obtain a positive culture. So actinomycosis should always be considered in a differential diagnosis of all infections of the cervicofacial area. Diagnosis of actinomycosis is made based on the histopathology, the clinical presentation and past dental history. We experienced a case of actinomycosis in the masseter muscle and present the case with review of literature.

• 대한세포병리학회지
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• 제16권1호
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• pp.57-60
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• 2005

A patient with actinomyces infection of the submandibular soft tissue was diagnosed by fine needle aspiration cytology (FNAC). A 38-year-old woman presented with a right submandibular mass which slowly grew in size over one month. Clinically and radiologically, the lesion was considered as tuberculous lymphadenitis or cellulitis. The polymerase chain reaction for tuberculosis was done by aspirated specimen but the result was negative. The smears of aspiration cytology showed characteristic colonies (sulfur granules) of actinomyces in inflammatory background. After antibiotic therapy for eight months, the patient has been well, showing no detectable mass. This patient was simply and rapidly diagnosed by FNAC and can avoid unnecessary surgical biopsy.

• International Journal of Oral Biology
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• 제41권4호
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• pp.209-215
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• 2016

Chlorhexidine has long been used in mouth washes for the control of dental caries, gingivitis and dental plaque. Minimal inhibitory concentration (MIC) is the lowest concentration of an antimicrobial substance to inhibit the growth of bacteria. Concentrations lower than the MIC are called sub minimal inhibitory concentrations (sub-MICs). Many studies have reported that sub-MICs of antimicrobial substances can affect the virulence of bacteria. The aim of this study was to investigate the effect of sub-MIC chlorhexidine on biofilm formation and coaggregation of oral early colonizers, such as Streptococcus gordonii, Actinomyces naeslundii and Actinomyces odontolyticus. The biofilm formation of S. gordonii, A. naeslundii and A. odontolyticus was not affected by sub-MIC chlorhexidine. However, the biofilm formation of S. mutans increased after incubation with sub-MIC chlorhexidine. In addition, cell surface hydrophobicity of S. mutans treated with sub-MIC of chlorhexidine, decreased when compared with the group not treated with chlorhexidine. However, significant differences were seen with other bacteria. Coaggregation of A. naeslundii with A. odontolyticus reduced by sub-MIC chlorhexidine, whereas the coaggreagation of A. naeslundii with S. gordonii remained unaffected. These results indicate that sub-MIC chlorhexidine could influence the binding properties, such as biofilm formation, hydrophobicity and coaggregation, in early colonizing streptococci and actinomycetes.

• 한국치위생학회지
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• 제15권4호
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• pp.735-741
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• 2015

Objectives: Lentinus edodes is an edible mushroom with a variety of beneficial effects such as antitumor, anti-inflammatory, antioxidant, and immune-modulatory activity. This study was carried out to evaluate the antimicrobial activities of Lentinus edodes extracts against oral-related bacteria. Methods: The antimicrobial activities of this extracts were investigated against S. anginosus, S. sobrinus, S. aureus, S. mutans, S. ratti, S. sanguinis, A. viscosus, A. naeslundii, and A. actinomycetemcomitans by the disc diffusion method, minimum inhibitory concentration (MIC), and growth inhibition. Results: Ethanol extracts had no antimicrobial activities, but acetone extracts showed antimicrobial activities against A. viscosus and A. actinomycetemcomitans and ethyl acetate extracts had effects against S. aureus, S. sanguinis, A. viscosus, and A. actinomycetemcomitans. Conclusions: The inhibitory effect of Lentinus edodes extracts was investigated on the growth of A. viscosus. Ethyl acetate and acetone extracts showed 90% and 77% inhibitory effect, respectively, against A. viscosus for 24 hrs. Ethyl acetate extracts had MIC of 25.0 mg/ml and acetone extracts showed MIC of >25.0 mg/ml.

• 한국식품조리과학회지
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• 제20권4호
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• pp.418-422
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• 2004

We investigated microbial populations of an upland and cultivated Codonopsis lanceolata. The microbial populations from both types of soils were also investigated. There were more than 10 microorganisms existed in upland than cultivated one. The total viable cell counts of C. lanceolata from upland and cultivated one, especially in the upper zone, were 9.7x10$\^$6/ CFU/g and 4.2${\times}$10$\^$6/ CFU/g, respectively. As a results, upper parts of C. lanceolata in upland were considered to harbour approximately more than 2.3 fold higher microorganisms than in cultivated one. However, the total viable cell counts between the two soil habitat, that is, 1.2${\times}$10$\^$7/ CFU/g from upland and 1.0x10$\^$7/ CFU/g from cultivated, were not significantly different. We also examined the unique flavor producing microorganisms in the soil extract broth including 25% C. lanceolata extract. One microorganism was detected in upper pars of C. lanceolata and upland soil. No. 6, microorganism causing the characteristic flavor of C. lanceolata was continued as Actinomyces by microscopy.