Browse > Article
http://dx.doi.org/10.14776/piv.2021.28.e12

Diagnostic Performance of Blood Culture Bottles for Abscess Culture in Patient with Abdominal Soft Tissue Abscess  

Jo, Kyo Jin (Department of Pediatrics, Pusan National University Children's Hospital)
Chang, Chulhun L. (Department of Laboratory Medicine, Pusan National University Yangsan Hospital)
Hwang, Jae-Yeon (Department of Radiology, Pusan National University Yangsan Hospital)
Park, Su Eun (Department of Pediatrics, Pusan National University Children's Hospital)
Publication Information
Pediatric Infection and Vaccine / v.28, no.2, 2021 , pp. 118-123 More about this Journal
Abstract
Culture tests are very important in choosing the appropriate antibiotics for bacterial infections. In some cases, bacteria that could not be identified in standard culture bottles could be detected using blood culture bottles. A previously healthy 13-year-old boy visited our emergency room. He experienced pain, redness, and hardness of periumbilical skin and a fever for five days. There was no history of abdominal surgery and penetrating trauma. Computed tomography showed abscess with cellulitis at the periumbilical soft tissue with no congenital anomaly. Ultrasonography-guided aspiration was performed, and about 8.5 mL of the purulent abscess was aspirated. The abscess was cultured using blood culture bottle. The pus grew Actinomyces radingae and Clostridium ramosum. When performing the pus culture, using blood culture bottles can be more effective and rapid than the standard culture method for the detection of bacterial pathogens.
Keywords
Actinomyces radingae; Clostridium ramosum; Culture technique; Diagnosis;
Citations & Related Records
연도 인용수 순위
  • Reference
1 American Academy of Pediatrics. Actinomycosis. In: Kimberlin DW, Brady MT, Jackson MA, Long SS, editors. Red book: 2018 report of the committee on infectious diseases. 31st ed. Itasca: American Academy of Pediatrics, 2018:205-6.
2 Sabbe LJ, Van De Merwe D, Schouls L, Bergmans A, Vaneechoutte M, Vandamme P. Clinical spectrum of infections due to the newly described Actinomyces species A. turicensis, A. radingae, and A. europaeus. J Clin Microbiol 1999;37:8-13.   DOI
3 Alexander CJ, Citron DM, Brazier JS, Goldstein EJ. Identification and antimicrobial resistance patterns of clinical isolates of Clostridium clostridioforme, Clostridium innocuum, and Clostridium ramosum compared with those of clinical isolates of Clostridium perfringens. J Clin Microbiol 1995;33:3209-15.   DOI
4 Li C, Ojeda-Thies C, Trampuz A. Culture of periprosthetic tissue in blood culture bottles for diagnosing periprosthetic joint infection. BMC Musculoskelet Disord 2019;20:299.   DOI
5 Kehrmann J, Chapot V, Buer J, Rating P, Bornfeld N, Steinmann J. Diagnostic performance of blood culture bottles for vitreous culture compared to conventional microbiological cultures in patients with suspected endophthalmitis. Eur J Clin Microbiol Infect Dis 2018;37:889-95.   DOI
6 Ng LS, Sim JH, Eng LC, Menon S, Tan TY. Comparison of phenotypic methods and matrix-assisted laser desorption ionisation time-of-flight mass spectrometry for the identification of aero-tolerant Actinomyces spp. isolated from soft-tissue infections. Eur J Clin Microbiol Infect Dis 2012;31:1749-52.   DOI
7 Heo EY, Shin S, Chung HS, Jeong YJ, Oh SH, Kim DK. The effect of using blood culture bottle of bronchoalveolar larvage fluid in pneumonia. BMC Infect Dis 2016;16:247.   DOI
8 Charoentunyarak S, Kananuraks S, Chindaprasirt J, Limpawattana P, Sawanyawisuth K. Blood culture bottle and standard culture bottle methods for detection of bacterial pathogens in parapneumonic pleural effusion. Jundishapur J Microbiol 2015;8:e24893.   DOI
9 Kononen E, Wade WG. Actinomyces and related organisms in human infections. Clin Microbiol Rev 2015;28:419-42.   DOI
10 Vandamme P, Falsen E, Vancanneyt M, Van Esbroeck M, Van de Merwe D, Bergmans A, et al. Characterization of Actinomyces turicensis and Actinomyces radingae strains from human clinical samples. Int J Syst Bacteriol 1998;48:503-10.   DOI
11 Senda S, Fujiyama Y, Ushijima T, Hodohara K, Bamba T, Hosoda S, et al. Clostridium ramosum, an IgA protease-producing species and its ecology in the human intestinal tract. Microbiol Immunol 1985;29:1019-28.   DOI
12 Legaria MC, Garcia SD, Tudanca V, Barberis C, Cipolla L, Cornet L, et al. Clostridium ramosum rapidly identified by MALDI-TOF MS. A rare gram-variable agent of bacteraemia. Access Microbiol 2020;2:acmi000137.
13 Brook I. Clostridial infection in children. J Med Microbiol 1995;42:78-82.   DOI