• Journal of Plant Biotechnology
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• 제32권3호
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• pp.187-193
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• 2005

톨 페스큐의 최적 조직배양 조건을 확립하기 위하여 성숙종자로부터 최적 배발생 캘러스유도조건 및 효율적인 식물체 재분화 체계를 확립하였다. 배발생 캘러스는 6 mg/L 2,4-D와 0.1 mg/L BA가 첨가된 MS배지에서 가장 높은 빈도로 유도되었으며, 식물체 재분화율도 증가시키는 것으로 나타났다. 식물체 재분화는 배발생 캘러스를 1 mg/L 2,4-D와 3 mg/L BA가 첨가된 N6배지에서 배양했을 때 50% 이상의 재분화율을 나타내었다. 기본배지의 종류에 따른 배양효율의 차이는 캘러스유도에는 MS배지가, 식물체 재분화에는 N6배지가 효과적이었다. 품종간 배발생 캘러스의 형성율과 식물체 재분화율은 Kentucky-31 품종이 각각 58.3%와 52%로서 가장 높은 효율을 나타내어 품종 간에 큰 차이를 나타내었다. 탄소원으로는 sucrose를 첨가해주었을 때 식물체 재분화율이 55%로 증가되었다. 본 연구를 통하여 확립된 단기간 고효율 재분화 시스템은 분자육종을 통한 신품종 톨 페스큐의 개발에 유용하게 응용되어질 수 있을 것이다.

• 상하수도학회지
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• 제36권2호
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• pp.121-134
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• 2022

In this study, the removal efficiency of PFCs(perfluorinated compounds) in the GAC(granule activated carbon) process based on the superheated steam automatic regeneration system was investigated in laboratory scale and pilot-scale reactor. Among PFCs, PFHxS(perfluorohexyl sulfonate) was most effectively removed. The removal efficiency of PFCs was found to be closely related to the EBCT, and the removal efficiencies of PFOA(perfluorooctanoic acid), PFOS(perfluorooctyl sulfonate), and PFHxS were 43.7, 75, and 100%, respectively, under the condition of EBCT of 6 min. Afterward, PFOA, PFOS, and PFHxS exhibited the earlier breakthrough time in the order. After that, GAC was regenerated, and the removal efficiency of the PFCs before and after regeneration was compared. As a result, it was shown that the PFCs removal efficiency in the regenerated GAC process were higher, and that of PFOA was improved to 75%. The findings of this study indicate the feasibility of the superheated steam automatic regeneration system for the stable removal of the PFCs, and it was verified that this technology can be applied stably enough even in field conditions.

• Journal of Plant Biotechnology
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• 제40권4호
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• pp.224-230
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• 2013

본 연구 결과 질산은과 구연산 및 비타민 C의 처리는 레드플레임의 갈변화 방지에는 효과적이나, 레드플레임 인편의 재분화 및 생장에 있어 비타민 C와 질산은의 처리는 효과적 이지 못하였다. 반면 구연산의 경우 레드플레임의 재분화 및 생장에 도움을 주는 것으로 나타났으나 $150mg{\cdot}l^{-1}$이상의 고농도에서는 생육이 저해됨을 보여 $100mg{\cdot}l^{-1}$ 처리가 나리의 재분화 및 생장에 가장 효율적임을 확인하였다. 또한 절편체의 갈변화를 감소시키는 부분에서는 비타민 C $150mg{\cdot}l^{-1}$ 처리가 1.5%의 가장 낮은 갈변율을 나타내어 갈변화 현상 감소에 가장 적합한 처리구로 판단되었다. 본 실험결과는 향후 나리기내 식물체생산에 문제점으로 발생하는 갈변화 현상을 감소시키고 인편 재분화 및 생장을 촉진시켜 우량 나리 기내 묘의 대량증식체계 확립을 통한 우량종구묘 생산체계 확립에 기여하여 농가 소득 향상에 도움이 될 것으로 판단된다.

• Plant Biotechnology Reports
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• 제3권4호
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• pp.341-345
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• 2009

This study describes culture conditions for a plant regeneration system via a combined pathway of somatic embryogenesis and organogenesis in root explant cultures of the commercial rose cultivar 'Charming'. Root explants formed white calluses at a frequency of 30% after 6 weeks of culture on Schenk and Hildebrandt (SH) medium supplemented with $11mg\;1^{-1}$ 2,4-dichlorophenoxyacetic acid. After 6 weeks of transfer to SH medium without growth regulators, initial white calluses gave rise to globular somatic embryos at a frequency of 2.8%, which were subsequently dedifferentiated to embryonic tissues. Somatic embryos or embryonic tissues initially derived from root explants did not undergo development beyond cotyledonary stage. To produce adventitious shoots, embryonic tissues were sliced and cultured on SH medium with $0.5mg\;1^{-1}$ 6-benzyladenine. After 4 weeks of culture, 28% of embryonic tissue explants formed adventitious shoots. Regenerated shoots were rooted on half strength SH medium with $0.1mg\;1^{-1}$ ${\alpha}-naphthalaneacetic$ acid and subsequently grown to maturity. Root-derived embryonic tissues were proliferated by subculture, while retaining the capacity for shoot production for a few years.

• Journal of Ginseng Research
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• 제23권3호
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• pp.148-163
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• 1999

The efficacy of three auxins, viz. 2,4-0, NAA and dicamba, were compared for the induction of somatic embryogenesis in American ginseng (Panax quinquefolium L.). Somatic embryos (SEs) formed on ginseng cotyledonary, zygotic embryo and shoot explants after 8 weeks of induction by the auxin stimuli. Significantly more somatic embryos were induced by culture of any of the ginseng explants on media supplemented with $5{\mu}M$ 2,4-0 than any other auxin treatment. Shoots derived from somatic embryos had the greatest regenerative potential and zygotic embryos the least. Explants generated from green (unstratified) seeds gave similar or higher frequency of embryogenesis as the explants derived from stratified seeds. Histological and SEM studies confirmed that the regenerimts were somatic embryos. Somatic embryos germinated and developed into normal plants in $3\~6$ months. About $10\%$ of plantlets from second generation SEs formed flowers within 10 weeks, particularly on media supplemented with $GA_3$ The development of a regeneration system for ginseng through somatic embryogenesis is a necessary first step for mass propagation and genetic improvement of American ginseng.

• 한국응용과학기술학회지
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• 제37권2호
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• pp.173-182
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• 2020

본 연구에서는 주류공장 내 고농도 CO₂ 제거 및 포집을 위해 L-alanine 흡수제를 적용하여 CO₂ 흡수, 탈리 효율을 평가 후 재생 성능을 확인하였다. 탈리공정의 효율적인 처리를 평가하기 위해 실험실 규모에서 Hot plate, Steam 두가지 탈리 방법을 비교하였으며 Hot plate는 약 10%, Steam의 경우 약 60%의 재생효율을 확인하였다. 따라서 주류공장 내 100㎥/min 실증화 규모에 Steam-Tower 탈리공정을 적용하여 최적조건을 평가한 결과 탈리 유량 4L/min 이하, Steam 온도 160℃ 이상, 탈리효율 85.5%로 확인할 수 있었다.

• 한국환경과학회지
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• 제18권6호
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• pp.645-654
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• 2009

Lab-scale Electrodialysis(ED) system with different membranes combined with before or after pyroma process were carried out to remove nitrate from two pickling acid wastewater containing high concentrations of $NO_3\;^-$(${\approx}$150,000 mg/L) and F($({\approx}$ 160,000 mg/L) and some heavy metals(Fe, Ti, and Cr). The ED system before Pyroma process(Sample A) was not successful in $NO_3\;^-$ removal due to cation membrane fouling by the heavy metals, whereas, in the ED system after Pyroma process(Sample B), about 98% of nitrate was removed because of relatively low $NO_3\;^-$ concentration (about 30,000 mg/L) and no heavy metals. Mono-selective membranes(CIMS/ACS) in ED system have no selectivity for nitrate compared to divalent-selective membranes(CMX/AMX). The operation time for nitrate removal time decreased with increasing the applied voltage from 10V to 15V with no difference in the nitrate removal rate between both voltages. Nitrate adsorption of a strong-base anion exchange resin of $Cl\;^-$ type was also conducted. The Freundlich model($R^2$ > 0.996) was fitted better than Langmuir mode($R^2$ > 0.984) to the adsorption data. The maximum adsorption capacity ($Q^0$) was 492 mg/g for Sample A and 111 mg/g for Sample B due to the difference in initial nitrate concentrations between the two wastewater samples. In the regeneration of ion exchange resins, the nitrate removal rate in the pickling acid wastewater decreased as the adsorption step was repeated because certain amount of adsorbed $NO_3\;^-$ remained in the resins in spite of several desorption steps for regeneration. In conclusion, the optimum system configuration to treat pickling acid wastewater from stainless-steel industry is the multi-processes of the Pyroma-Electrodialysis-Ion exchange.

• Plant Resources
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• 제8권3호
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• pp.286-292
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• 2005

Shoot induction system was developed in the recalcitrant plant species, Brassica rapa ssp. rapifera by using optimum selection of profit organ, phytohormone combination, seedling age and kind of culture container. Out of in vitro cultured leaf segment, petiole, hypocotyl, and cotyledon with petiole, only cotyledon with petiole derived from 4 day-old seedlings induced multiple shoot. The optimum combination of auxin and cytokinin for the multiple shoot induction was MS medium containing 5mg/L BA and 0.5mg/L NAA. The major factors for multiple shoot propagation were part of plant organ, age of seedling, and ratio of auxin and cytokinin. In addition, shoot regeneration was promoted in the 100ml Erlenmeyer flask compared with the $90mm{\times}20mm$ Petri-dish. The induced shoots formed roots easy on MS medium containing 0.1mg/L IBA and the whole plants were successfully cultivated in soil.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제35권3호
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• pp.137-145
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• 2009

As an efficient controlled release system for rhBMP-2, a functional nanoparticle-hydrogel complex, incorporated with matrix metalloproteinase(MMP) sensitive peptide cross-linker, was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase(ALP) activity and mineral contents analysis, based on the rat calvarial critical size defect(8mm in diameter) model. Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded MMP sensitive hyaluronic acid(HA) based hydrogel-Nanoparticles(NP) complex, as compared to only MMP HA, the MMP HA-NP without rhBMP-2, or even with the rhBMP-2. These improvements included the formation pattern of bone and functional marrow, the degree of calcium quantification, and the ALP activity. These results indicate that the MMP sensitive HA with nano-particle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced rhBMP-2 scaffold.

• Journal of Microbiology and Biotechnology
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• 제23권2호
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• pp.218-224
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• 2013

The aldo-keto reductases catalyze reduction reactions using various aliphatic and aromatic aldehydes/ketones. Most reductases require NADPH exclusively as their cofactors. However, NADPH is much more expensive and unstable than NADH. In this study, we attempted to change the five amino acid residues that interact with the 2'-phosphate group of the adenosine ribose of NADPH. These residues were selected based on a docking model of the YOL151W reductase and were substituted with other amino acids to develop NADH-utilizing enzymes. Ten mutants were constructed by site-directed mutagenesis and expressed in Escherichia coli. Among them, four mutants showed higher reductase activities than wild-type when using the NADH cofactor. Analysis of the kinetic parameters for the wild type and mutants indicated that the $k_{cat}/K_{m}$ value of the Asn9Glu mutant toward NADH increased 3-fold. A docking model was used to show that the carboxyl group of Glu 9 of the mutant formed an additional hydrogen bond with the 2'-hydroxyl group of adenosine ribose. The Asn9Glu mutant was able to produce (R)-ethyl-4-chloro-3-hydroxyl butanoate rapidly when using the NADH regeneration system.