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BONE REGENERATION WITH MMP SENSITIVE HYALURONIC ACID-BASED HYDROGEL, rhBMP-2 AND NANOPARTICLES IN RAT CALVARIAL CRITICAL SIZE DEFECT(CSD) MODEL  

Nam, Jeong-Hun (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University)
Park, Jong-Chul (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University)
Yu, Sang-Bae (Dental Research Institute, School of Dentistry, Seoul National University)
Chung, Yong-Il (Department of Materials Science and Engineering, Gwangju Institute of Science and Technology)
Tae, Gi-Yoong (Department of Materials Science and Engineering, Gwangju Institute of Science and Technology)
Kim, Jung-Ju (Department of Biomedical Engineering, Biomedical Engineering, Brain Korea 21 2nd Project for Biomedical Science)
Park, Yong-Doo (Department of Biomedical Engineering, Biomedical Engineering, Brain Korea 21 2nd Project for Biomedical Science)
Jahng, Jeong-Won (Dental Research Institute, School of Dentistry, Seoul National University)
Lee, Jong-Ho (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University)
Publication Information
Journal of the Korean Association of Oral and Maxillofacial Surgeons / v.35, no.3, 2009 , pp. 137-145 More about this Journal
Abstract
As an efficient controlled release system for rhBMP-2, a functional nanoparticle-hydrogel complex, incorporated with matrix metalloproteinase(MMP) sensitive peptide cross-linker, was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase(ALP) activity and mineral contents analysis, based on the rat calvarial critical size defect(8mm in diameter) model. Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded MMP sensitive hyaluronic acid(HA) based hydrogel-Nanoparticles(NP) complex, as compared to only MMP HA, the MMP HA-NP without rhBMP-2, or even with the rhBMP-2. These improvements included the formation pattern of bone and functional marrow, the degree of calcium quantification, and the ALP activity. These results indicate that the MMP sensitive HA with nano-particle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced rhBMP-2 scaffold.
Keywords
matrix metalloproteinase(MMP); hyaluronic acid; controlled release; rhBMP-2; nanoparticle; MMP sensitive hydrogel;
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