Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Efficient Callus Culture and Plant Regeneration from Mature Seed of Tall Fescue (Festuca arundinacea Schreb.)

톨 페스큐의 성숙종자로부터 효율적인 캘러스 배양 및 식물체 재분화

  • Kim Do-Hyun (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee Dong-Gi (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee Sang-Hoon (Division of Applied Life Science, Gyeongsang National University) ;
  • Woo Hyun-Sook (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee Ki-Won (Division of Applied Life Science, Gyeongsang National University) ;
  • Choi Myung-Suk (Division of Forest Science, Gyeongsang National University) ;
  • Lee Byung-Hyun (Division of Applied Life Science, Gyeongsang National University)
  • 김도현 (경상대학교 응용생명과학부) ;
  • 이동기 (경상대학교 응용생명과학부) ;
  • 이상훈 (경상대학교 응용생명과학부) ;
  • 우현숙 (경상대학교 응용생명과학부) ;
  • 이기원 (경상대학교 응용생명과학부) ;
  • 최명석 (경상대학교 산림자원과학부) ;
  • 이병현 (경상대학교 응용생명과학부)
  • Published : 2005.09.01
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Abstract

In an effort to optimize tissue culture conditions for genetic transformation of tall fescue (Festuca arundinacea Schreb.), an efficient plant regeneration system from seed-derived calli was established. MS medium containing 6 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.1 mg/L benzyladenine (BA) were optimal for embryogenic callus formation from mature seed and had a strong effect on successive plant regeneration. The plant regeneration frequency above 50% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. 'Kentucky-31' showed to have high frequencies of embryogenic callus induction and plant regeneration up to 58.3 and 50%, respectively. Addition of sucrose to the regeneration medium as a carbon source increased regeneration frequency up to 55%. A short tissue culture period and high-frequency regeneration system established in this study will be useful for molecular breeding of tall fescue through genetic transformation.

톨 페스큐의 최적 조직배양 조건을 확립하기 위하여 성숙종자로부터 최적 배발생 캘러스유도조건 및 효율적인 식물체 재분화 체계를 확립하였다. 배발생 캘러스는 6 mg/L 2,4-D와 0.1 mg/L BA가 첨가된 MS배지에서 가장 높은 빈도로 유도되었으며, 식물체 재분화율도 증가시키는 것으로 나타났다. 식물체 재분화는 배발생 캘러스를 1 mg/L 2,4-D와 3 mg/L BA가 첨가된 N6배지에서 배양했을 때 50% 이상의 재분화율을 나타내었다. 기본배지의 종류에 따른 배양효율의 차이는 캘러스유도에는 MS배지가, 식물체 재분화에는 N6배지가 효과적이었다. 품종간 배발생 캘러스의 형성율과 식물체 재분화율은 Kentucky-31 품종이 각각 58.3%와 52%로서 가장 높은 효율을 나타내어 품종 간에 큰 차이를 나타내었다. 탄소원으로는 sucrose를 첨가해주었을 때 식물체 재분화율이 55%로 증가되었다. 본 연구를 통하여 확립된 단기간 고효율 재분화 시스템은 분자육종을 통한 신품종 톨 페스큐의 개발에 유용하게 응용되어질 수 있을 것이다.

Keywords

References

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