• Asian-Australasian Journal of Animal Sciences
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• v.29 no.3
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• pp.343-351
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• 2016

Although the chemical, physical, and nutritional properties of bovine milk have been extensively studied, only a few studies have attempted to characterize milk-synthesizing genes using RNA-seq data. RNA-seq data was collected from 21 Holstein samples, along with group information about milk production ability; milk yield; and protein, fat, and solid contents. Meta-analysis was employed in order to generally characterize genes related to milk production. In addition, we attempted to investigate the relationship between milk related traits, parity, and lactation period. We observed that milk fat is highly correlated with lactation period; this result indicates that this effect should be considered in the model in order to accurately detect milk production related genes. By employing our developed model, 271 genes were significantly (false discovery rate [FDR] adjusted p-value<0.1) detected as milk production related differentially expressed genes. Of these genes, five (albumin, nitric oxide synthase 3, RNA-binding region (RNP1, RRM) containing 3, secreted and transmembrane 1, and serine palmitoyltransferase, small subunit B) were technically validated using quantitative real-time polymerase chain reaction (qRT-PCR) in order to check the accuracy of RNA-seq analysis. Finally, 83 gene ontology biological processes including several blood vessel and mammary gland development related terms, were significantly detected using DAVID gene-set enrichment analysis. From these results, we observed that detected milk production related genes are highly enriched in the circulation system process and mammary gland related biological functions. In addition, we observed that detected genes including caveolin 1, mammary serum amyloid A3.2, lingual antimicrobial peptide, cathelicidin 4 (CATHL4), cathelicidin 6 (CATHL6) have been reported in other species as milk production related gene. For this reason, we concluded that our detected 271 genes would be strong candidates for determining milk production.

• Journal of Sensor Science and Technology
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• v.14 no.1
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• pp.33-41
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• 2005

For more convenient electrode-electrolyte interface impedance analysis in biosensor, a stand-alone impedance measurement system is required. In our study, we developed a PC-based portable system to analyze impedance of the electrochemical cell using microprocessor. The devised system consists of signal generator, programmable amplifiers, A/D converter, low pass filter, potentiostat, I/V converter, microprocessor, and PC interface. As a microprocessor, PIC16F877 which has the processing speed of 5 MIPS was used. For data acquisition, the sampling rate at 40 k samples/sec, resolution of 12 bit is used. RS-232 with 115.2 kbps speed is used for the PC communication. The square wave was used as stimuli signal for impedance analysis and voltage-controlled current measurement method of three-electrode-method were adopted. Acquired voltage and current data are calculated to multifrequency impedance signal after Fourier transform. To evaluate the implemented system, we set up the dummy cell as equivalent circuit of which was composed of resistor, parallel circuit of capacitor and resistor connected in parallel and measured the impedance of the dummy cell; the result showed that there exist accuracy within 5 % errors and reproduction within 1 % errors compared to output of Hioki LCR tester and HP impedance analyzer as a standard product. These results imply that it is possible to analyze electrode-electrolyte interface impedance quantitatively in biosensor and to implement the more portable high speed impedance analysis system compared to existing systems.

• Journal of the Korean Association of Geographic Information Studies
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• v.3 no.2
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• pp.48-60
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• 2000

This study is aimed to clarify the distribution chracteristics of marine terraces and extract the specific surface at Gampo-eup, Gyeongju city on the map of 1:5,000 using GIS. The effects and problems occurred on the process of using GIS were investigated for the research of marine terrace. The longitudinal profile analysis was carried out along the 12 sections on the geomorphic surfaces of the study area, and actually High higher surface(HH-surface) was found over 100m a.s.l., which has not been reported till now. And the occupancy rate could be calculated by substitution on the height between 4m and 87m a.s.l. for each mean slope degree $1-5^{\circ}$ obtained from the actual measuring along four sections. Consequently the lower-I surface was highly reliable to use as the key bed for studying marine terraces. The accurate and detail analysis about the marine terraces is able to be accomplished on the basis of meaningful actual measuring, though its general possible distribution area can be extracted from GIS with the less effort. Namely the quantified results obtained from GIS could offer the basis for the objective analysis of the geomorphic surfaces. And we can look over the landscape and investigate the surfaces with reliefs effectively in relation to the real geomorphology on the study area, where in situ approach is difficult. But the digital map with a large scale should be offered first of all in order to raise the accuracy of the analysis.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.12
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• pp.1713-1720
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• 2015

The present work describes experiments undertaken to evaluate the usefulness of selected physicochemical indices of semen, cell membrane integrity and sperm chromatin structure for the assessment of boar semen sensitivity to processes connected with pre-insemination procedures. The experiments were carried out on 30 boars: including 15 regarded as providers of sensitive semen and 15 regarded as providers of semen that is little sensitive to laboratory processing. The selection of boars for both groups was based on sperm morphology analyses, assuming secondary morphological change incidence in spermatozoa as the criterion. Two ejaculates were manually collected from each boar at an interval of 3 to 4 months. The following analyses were carried out for each ejaculate: sperm motility assessment, sperm pH measurement, sperm morphology assessment, sperm chromatin structure evaluation and cell membrane integrity assessment. The analyses were performed three times. Semen storage did not cause an increase in the incidence of secondary morphological changes in the group of boars considered to provide sperm of low sensitivity. On the other hand, with continued storage there was a marked increase in the incidence of spermatozoa with secondary morphological changes in the group of boars regarded as producing more sensitive semen. Ejaculates of group I boars evaluated directly after collection had an approximately 6% smaller share of spermatozoa with undamaged cell membranes than the ejaculates of boars in group II ($p{\leq}0.05$). In the process of time the percentage of spermatozoa with undamaged cell membranes decreased. The sperm of group I boars was characterised with a lower sperm motility than the semen of group II boars. After 1 hour of storing diluted semen, the sperm motility of boars producing highly sensitive semen was already 4% lower ($p{\leq}0.05$), and after 24 hours of storage it was 6.33% lower than that of the boars that produced semen with a low sensitivity. Factors that confirm the accuracy of insemination male selection can include a low rate of sperm motility decrease during the storage of diluted semen, low and contained incidence of secondary morphological changes in spermatozoa during semen storage and a high frequency of spermatozoa with undamaged cell membranes.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.26 no.6
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• pp.397-404
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• 2014

In Korea, harbor tranquility is generally estimated by using both methods of investigating harbor calmness under abnormal wave condition and evaluating the harbor serviceability. The efficiency of the former method is questionable as the tranquility of a harbor is judged by a wave height criterion that is arbitrarily determined without rational basis. In case of the latter method, the utilization rate of a harbor is estimated by using the exceedance probability of wave height or the distribution of wave heights and periods that is obtained from longterm measured or hindcasted wave data. Use of long-term data is desirable in order to guarantee the accuracy of the exceedance probability. Meanwhile, the criterion for determining maximum allowable wave height for cargo handling works is too simple and has limitations for being used in an actual field condition. Problems of existing method for estimating harbor tranquility were verified by the wave observation data in Busan New Port. And the importance of the field observation data was emphasized. It is necessary to perform long-term wave monitoring inside and outside of major ports in Korea in order to establish more advanced standard for evaluating harbor tranquility based on such observed wave data.

• Journal of Energy Engineering
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• v.5 no.2
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• pp.160-169
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• 1996

Parametric studies are conducted for optimizing the integration design between gas turbine compressor and air separation unit (ASU) of integrated gasification combined cycle power plant. The present study adopts the ASU of double-distillation column process, from which integration conditions with compressor such as the heat exchanger condition between air and nitrogen, the amount and the pressure of extracted air are defined and mathematically formulated. The performance variations of the compressor integrated with ASU are analyzed by combining streamline curvature method and pressure loss models, and the predicted results are compared with the performance test results of actual compressors to verify the prediction accuracy. Using the present performance prediction method, the effects of pinch-point temperature difference (PTD) in the heat exchanger, the amount and the pressure of extracted air on compressor performances are quantitatively examined. As the extraction air amount or the PTD is increased, the pressure ratio and the power consumption of compressor are increased. The compressor efficiency deteriorates as the increase of the flow rate of air extracted at higher pressure level while improving at lower pressure air extraction. Furthermore, through the characteristic curve between generalized inlet condition and efficiency of compressor, optimal integration condition is presented to maximize the compressor efficiency.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.33 no.2
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• pp.83-94
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• 2015

Recently, the necessity of spatial data in unaccessible area was challenged to set up various plans and policies for preparing the unification and the cooperative projects between South-North Korea. Therefore, this paper planned to evaluate the possibility of adopting the error tolerance in Geometric correction for 1/5,000 digital topographic mapping, using the PLEIADES images and the TerraSAR GCPs (Ground Control Points). The geometric correction was performed by changing the number and placement of GCPs by GPS (Global Positioning System) surveying, as the optimal placement of 5 GCPs were selected considering the geometric stability and steady rate. The positional accuracy evaluated by the TerraSAR GCPs, which were selected by optimal placement of GCPs. The RMSE in control points were X=±0.64m, Y=±0.46m, Z=±0.28m. While the result of geometric correction for PLEIADES images confirmed that the RMSE in control points were X=±0.34m, Y=±0.27m, Z=±0.11m, the RMSE in check points were X=±0.50m, Y=±0.30m, Z=±0.66m. Through this study, we believe if spatial data can integrate with the PLEIADES images and the optimal TerraSAR GCPs, it will be able to obtain the high-precision spatial data for adopting the regulation of 1/5,000 digital topographic map, which adjusts the computation as well as the error bound.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.31 no.6_1
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• pp.483-491
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• 2013

By a development of car navigation systems and mobile or positioning technology, it increases interest in location based services, especially pedestrian navigation systems. Updating of digital maps is important because digital maps are mass data and required to short updating cycle. In this paper, we proposed change detection for different network data-sets based on areal feature matching. Prior to change detection, we defined type of updating between different network data-sets. Next, we transformed road lines into areal features(block) that are surrounded by them and calculated a shape similarity between blocks in different data-sets. Blocks that a shape similarity is more than 0.6 are selected candidate block pairs. Secondly, we detected changed-block pairs by bipartite graph clustering or properties of a concave polygon according to types of updating, and calculated Fr$\acute{e}$chet distance between segments within the block or forming it. At this time, road segments of KAIS map that Fr$\acute{e}$chet distance is more than 50 are extracted as updating road features. As a result of accuracy evaluation, a value of detection rate appears high at 0.965. We could thus identify that a proposed method is able to apply to change detection between different network data-sets.

• Journal of the Korean Society for Precision Engineering
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• v.26 no.2
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• pp.101-108
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• 2009

A fretting wear test rig for dry ambient condition, which employs a piezoelectric actuator, has been developed. It is driven and loaded in a very simple manner with acceptable experimental accuracy. By using the rig, Inconel 690 tube has been tested under the normal load of 10 and 15N with sliding amplitude of less than $100{\mu}m$ during $10^6$cycles. The wear resistance of the material has been characterized in terms of the wear coefficient based on the work rate model. SEM micrographs show the complex structures of the scars, which consist of risen peaks, plate-type thin layers and locally exposed bare surfaces. The cracks spread over the layers give clue to the fretting wear mechanism of the material.

• Journal of Korea Water Resources Association
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• v.42 no.6
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• pp.457-464
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• 2009

This paper proposes methodologies for analyzing the accuracy of the proportional hazards model in predicting consecutive break times of water mains and estimating the time interval for economical water main replacement. By using the survival functions that are based on the proportional hazards models a criterion for the prediction of the consecutive pipe breaks is determined so that the prediction errors are minimized. The criterion to predict pipe break times are determined as the survival probability of 0.70 and only the models for the third through the seventh break are analyzed to be reliable for predicting break times for the case study pipes. Subsequently, the criterion and the estimated lower and upper bound survival functions of consecutive breaks are used in predicting the lower and upper bounds of the 95% confidence interval of future break times of an example water main. Two General Pipe Break Prediction Models(GPBMs) are estimated for an example pipe using the two series of recorded and predicted lower and upper bound break times. The threshold break rate is coupled with the two GPBMs and solved for time to obtain the economical replacement time interval.