• Korean Journal of Medicinal Crop Science
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• v.27 no.2
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• pp.96-107
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• 2019

Background: The aim of this study was to compare the fatty acid composition, antioxidant activity, and the content of two major phenolic acids, caffeic and rosmarinic acids, of 45 Perilla accessions collected from Russia. Methods and Results: A total of 45 accessions of the genus Perilla were used in this study. The antioxidant activities of these accessions were analyzed using a spectrophotometer, and their caffeic and rosmarinic acid contents were determined using a reversed-phase ultraperformance liquid chromatography (UPLC) system. The Perilla seed oil was recovered using hexane in a soxhlet extraction method. The fatty acid compositions were analyzed using a Shimadzu QP2010 gas chromatography-mass spectoroscopy system. The results showed that accessions IT226732 and IT274300 had the highest content of caffeic and rosmarinic acid, respectively. A cluster analysis based on antioxidant assay results and concentration of phenolic acids led to the categorization of Perilla accessions into three major groups. The accessions in Group I were characterized by higher caffeic and rosmarinic acid content and antioxidant activity, compared to the accessions in the other two groups (p < 0.05). The total oil content ranged between 28.39 and 46.89%. The compositions of oleic, linoleic, and linolenic acid ranged from 11.83 to 19.55%, 11.92 to 16.71%, and 59.19 to 67.28%, respectively. Conclusions: The results of this study indicated that accessions IT274300, IT226732, IT274293, IT235818, and IT235820 could be used as a source of functional materials.

• Korean Journal of Plant Resources
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• v.34 no.5
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• pp.478-490
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• 2021

We aimed to generate basic breeding data for melon (Cucumis melo L.). A total of 219 melon accessions conserved at the National Agrobiodiversity Center (NAC) in Rural Development Administration (RDA) were used in this study, of which 72 (33%) were collected from India. The majority of accessions showed orange (42%) and white (36%) flesh color. In addition to phenotypic evaluations, the accessions were genotyped using a molecular marker for the carotenoid biosynthesis gene CmOr. DNA fragments of the expected size were amplified in 205 out of 219 accessions. Digestion of the PCR products with HinfI restriction endonuclease showed 100% concordance between phenotype and genotype in green-fleshed accessions, but 98%, 97%, and 80% concordance in orange-, white-, and creamy-fleshed accessions, respectively. Sequence analysis revealed single nucleotide changes in the three positions of SNP1, SNP2 and SNP1int in the CmOr gene among accessions. These newly found alleles suggest that there are multiple mechanisms in determining fruit flesh color in melon. Also, the phenotype data of diverse accessions obtained in this study will be a valuable source for melon breeding.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.4
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• pp.336-346
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• 2013

Amaranth (Amaranthus sp. L.) is an important group of plants that includes grain, vegetable, and ornamental types. Centers of diversity for Amaranths are Central and South America, India, and South East Asia, with secondary centers of diversity in West and East Africa. The present study was performed to determine the genetic diversity and population structure of 75 amaranth accessions: 65 from South America and 10 from South Asia as controls using 14 SSR markers. Ninety-nine alleles were detected at an average of seven alleles per SSR locus. Model-based structure analysis revealed the presence of two subpopulations and 3 admixtures, which was consistent with clustering based on the genetic distance. The average major allele frequency and polymorphic information content (PIC) were 0.42 and 0.39, respectively. According to the model-based structure analysis based on genetic distance, 75 accessions (96%) were classified into two clusters, and only three accessions (4%) were admixtures. Cluster 1 had a higher allele number and PIC values than Cluster 2. Model-based structure analysis revealed the presence of two subpopulations and three admixtures in the 75 accessions. The results of this study provide effective information for future germplasm conservation and improvement programs in Amaranthus.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.68-68
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• 2018

A total of 47 core collections of pepper (Capsicum chinense Jacq.) conserved in National Agrobiodiversity Center (NAC) were studied under field condition at Jeonju. All accessions were characterized for their 14 qualitative and 16 quantitative characters. Results revealed that both qualitative and quantitative characters exhibited wide variation among the studied germplasm. Distribution of fruit characters (fruit length, width, and fruit wall thickness) among the accessions was positively skewed. Of the 47 accessions evaluated, 38.3% accessions had conical shaped fruits and mature fruit color was predominantly red (51.1%), orange (21.3%) and yellow (14.9%). Principal component analyses revealed that (i) 56.64% of the qualitative (fruit shape, color and fruit surface) variation and (ii) 89.42% of the quantitative (plant width, height and fruit maturity days) variation were explained by the first two components. Clustering revealed two groups and dendrogram revealed morphological variation among accessions. The phenotypic diversity exists in this core collections provide valuable information to improve agronomic traits in pepper breeding program.

• Korean Journal of Medicinal Crop Science
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• v.21 no.3
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• pp.220-228
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• 2013

This study was conducted to assess the genetic diversity and population structure of 70 amaranth accessions collected from South and Southeast Asia using 14 simple sequence repeat (SSR) markers. In total, 67 alleles were detected, with an average of 4.79 per locus. Rare alleles comprised a large portion (46.3%) of the detected alleles, and 29 unique alleles associated with rice accessions were also discovered. The mean major allele frequency (MAF), genetic diversity (GD) and polymorphic information content (PIC) of the 14 SSR loci were 0.77, 0.36, and 0.34, respectively. A model-based structural analysis revealed the presence of three subpopulations. The genetic relationships revealed by the neighbor-joining tree method were fairly consistent with the structure-based membership assignments for most of the accessions. All 70 accessions showed a clear relationship to each cluster without any admixtures. We observed a relatively low extent of genetic exchange within or among amaranth species from South and Southeast Asia. The genetic diversity results could be used to identify amaranth germplasms and so facilitate their use for crop improvement.

• Journal of Crop Science and Biotechnology
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• v.10 no.1
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• pp.27-32
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• 2007

RAPD, Inter-SSR, and AFLP markers were used to assess the genetic diversity of lettuce cultivars and the phylogenetic relationships in Lactuca spp. A total of 216 polymorphic bands from seven RAPD primers, four Inter-SSR primers, and five AFLP primer combinations were used to elucidate the genetic similarity among lettuce cultivars. Forty-four lettuce accessions were subdivided into discrete branches according to plant type: crisphead, butterhead, and stem type, with some exceptions. The leafy- and cos-type accessions were intermingled in other groups with no discrete branch indicating that these are more diverse than others. Three accessions, including the Korean cultivar 'Cheongchima', the Korean local landrace 'Jinjam', and the German cultivar 'Lolla Rossa' were classified as the most diverse accessions. Twenty bands were unique in specific cultivars. Among these, three were specific in a plant type; one in Korean leafy type, one in crisphead type, and one in cos type lettuce. In the phylogenetic analysis among Lactuca species, L. saligna, L. serriola, and L. georgica clustered in a sister branch of the L. sativa complex. Two L. virosa accessions show the highest intra-specific relationships. L. perennis outlied from all the other Lactuca species at a genetic similarity of 0.53 and clustered with two Cichorium species, C. intybus and C. endivia, with genetic similarity of 0.67. The phylogenetic tree was supported by data from polymorphism of chloroplast genome which was revealed by PCR-RFLP.

• Korean Journal of Medicinal Crop Science
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• v.19 no.3
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• pp.149-156
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• 2011

The genetic diversity and the genetic relationship among 30 genetic resources of T. officinale and T. coreanum collected from 20 regions in Korea were evaluated by using ISSR markers. Out of 127 loci detected overall, 122 were identified to be polymorphic with a rate of 96.0% at the 30 individuals. The intraspecific polymorphism between T. officinale and T. coreanum was 92.6% and 88.2%, respectively. The genetic similarity matrix (GSM) revealed a wide range of variablility among the 30 accessions, spanning from 0.179 to 922. According to the clustering analysis, different species T. officinale and T. coreanum, were divided into independent groups and all of the accessions could be classified into 7 categories. Especially, all of the mountain collected accessions belonged to independent groups. The study findings indicate that T. officinale and T. coreanum accessions have a high genetic diversity and accordingly carry a germ-plasm qualifying as good genetic resources for breeding.

• International Journal of Industrial Entomology and Biomaterials
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• v.14 no.2
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• pp.87-92
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• 2007

Twenty - four elite mulberry germplasm each of indigenous and exotics were studied for their leaf yield and compared with commercial check ($V_1$ and Kosen). Accession MI-0416 and ME-0169 out yielded the check accession in leaf yield/plant. The other few mulberry germplasm were also performed at par with the checks. For quality test and bioassay were conducted with the leaves of selected mulberry germplasm. Among the selected twelve mulberry accessions used for bioassay, MI-0376 and ME-033 performed better than check ($V_1$, Kosen). Other mulberry accessions i.e., MI-0310 and MI-0437 are on par with the check as far as the bioassay is concerned. MI-0376 and ME-0033 out yielded in rearing parameters and qualified for 11 and 10 rearing and related traits. Other mulberry accessions i.e., MI-0310 and MI-0437 were also qualified for eight rearing traits along with check ($V_1$). The mulberry accessions tested after selection from the preliminary characterization seems to be better and equally good in rearing and leaf yield compared to check ($V_1$, Kosen), which provides scope for selection and further evaluation. The selected mulberry accession may be included in crop improvement programme.

• Korean Journal of Medicinal Crop Science
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• v.17 no.3
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• pp.165-172
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• 2009

The bramble cultivated widely in South Korea, which is known as Bokbunja-ddal-gi, is regarded as having originated from Korean native Rubus coreanus. This study was carried out to obtain basic phylogenetic information on Korean cultivated bramble (KCB) by comparing the internal transcribed spacer (ITS) regions with those of R. coreanus, blackberry (R. lanciniatus), black (R. occidentalis) and red (R. idaeus) raspberry. Sequences of the ITS 1 suggest that some KCB accessions share a significant similarity with both R. occidentalis and R. coreanus in the ITS 1 region. The ITS 2 sequences of the three KCB accessions clustered more closely to those of two R. occidentalis accessions than to those of R. coreanus. These results suggest that there exist variations in the sequences of ITS among KCB accessions and KCB accessions are more closely related to black raspberry than R. coreanus in the ITS regions.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.38-38
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• 2019

Among the 6500 accessions of the pepper germplasm which conserved at RDA Genebank, 319 accessions were investigated charateristics such as their growth habit, flowering and fruit shape in Capsicum frutescens. All accessions were characterized for their 14 qualitative and 16 quantitative characters. Results revealed that both qualitative and quantitative characters exhibited wide variation among the studied germplasm. The hypocotyl color, which investigated in juvenile stage, was divided in three types such as green, purple, and purple with green, and most of the accessions showed in purple. Flowering starts from 7 May, and most accessions were starting to flower from 27 May to 25 June. The fresh color of the mature pepper were red, yellow, orange, Etc. Fruit shape were mostly elongated, while exhibited ball shape and round shape were a few. The fresh weight of fruits ranged from 0.2 to 42.3, and the diameter of fruit showed from 4.2 to 45.1 mm, respectively. The phenotypic diversity exists in Capsicum frutescens. provide valuable information to improve agronomic traits in pepper breeding program.