Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Genetic Diversity and Relationship Analysis of Taraxacum officinale Weber and Taraxacum coreanum Nakai Accessions Based on Inter-Simple Sequence Repeats (ISSR) Markers

ISSR 표지에 의한 서양민들레와 흰민들레 수집종의 유전적 다양성 및 유연관계 분석

  • Ryu, Jai-Hyunk (Department of Bioresources Science, Sunchon National University) ;
  • Bae, Chang-Hyu (Department of Bioresources Science, Sunchon National University)
  • 류재혁 (순천대학교 생명산업과학대학 웰빙자원학과) ;
  • 배창휴 (순천대학교 생명산업과학대학 웰빙자원학과)
  • Received : 2011.05.16
  • Accepted : 2011.06.14
  • Published : 2011.06.30
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Abstract

The genetic diversity and the genetic relationship among 30 genetic resources of T. officinale and T. coreanum collected from 20 regions in Korea were evaluated by using ISSR markers. Out of 127 loci detected overall, 122 were identified to be polymorphic with a rate of 96.0% at the 30 individuals. The intraspecific polymorphism between T. officinale and T. coreanum was 92.6% and 88.2%, respectively. The genetic similarity matrix (GSM) revealed a wide range of variablility among the 30 accessions, spanning from 0.179 to 922. According to the clustering analysis, different species T. officinale and T. coreanum, were divided into independent groups and all of the accessions could be classified into 7 categories. Especially, all of the mountain collected accessions belonged to independent groups. The study findings indicate that T. officinale and T. coreanum accessions have a high genetic diversity and accordingly carry a germ-plasm qualifying as good genetic resources for breeding.

Keywords

References

  1. Ahn YH and Chung KH. (2003). Analysis of genetic variance among Taraxacum officinale growing in each populated areas using RAPD. Korean Journal of Environment and Ecology. 17:27-31.
  2. Ahn YH, Park DS and Chung KH. (2003). Analysis of genetic relationship among native Taraxacum and naturalized Taraxacum species using RAPD. Korean Journal of Environment and Ecology. 17:169-176
  3. Baarlen P van, de Jong JH and van Dijk PJ. (2002). Comparative cyto-embryological investigations of sexual and apomictic dandelions (Taraxacum) and their apomictic hybrids. Sex Plant Reproduction. 15:31-38. https://doi.org/10.1007/s00497-002-0132-x
  4. Battjes J, Menken SBJ and den Nijs HCM. (1992). Clonal diversity in some microspecies of Taraxacum sect. Palustria (Lindb. fil.) Dahlst. from Czechoslowakia. Botanische Jahrbucher fur Systematik, Pflanzengeschichte and Pflanzengeographie. 114:315-328.
  5. Bartish IV, Hylmo B and Nybom H. (2001). RAPD analysis of interspecific relationships in presumably apomictic Cotoneaster species. Euphytica. 120:273-280. https://doi.org/10.1023/A:1017585600386
  6. Christoph R. (2004). Molecular differentiation between coexisting species of Taraxacum sect. Erythrosperma (Asteraceae) from populations in south-east and west Germany. Botanical Journal of the Linnean Society. 142:109-117.
  7. Esselman E, Jiangquiang JL, Crawford DJ, Winduss JL and Wolfe AD. (1999). Clonal diversity in the rare Calamagrosis porteri spp. insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and their simple sequence repeat (ISSR) markers. Molecular Ecology. 8:443-451. https://doi.org/10.1046/j.1365-294X.1999.00585.x
  8. Fang DQ and Roose ML. (1997). Identification of closely related citrus cultivars with inter-simple sequence markers. Theoretical and Applied Genetics. 95:408-417. https://doi.org/10.1007/s001220050577
  9. Fang G, Hammar S and Grumet R. (1992). A quick inexpensive method of removing ploysaccharides from plant genomic DNA. Biotechniques. 13:52-55.
  10. Godwin ID, Aiken EAB and Smith LW. (1997). Application of inter simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis. 18:1524-1528. https://doi.org/10.1002/elps.1150180906
  11. Hulst RGM van D, Mes THM, Den Nijs JCM and Bachmann K. (2000). Amplified fragment length polymorphism (AFLP) markers reveal that population structure of triploid dandelions (Taraxacum officinale) exhibits both clonality and recombination. Molecular Ecology. 9:1-8. https://doi.org/10.1046/j.1365-294x.2000.00704.x
  12. Iruela M, Rubio J, Cubero JI, Gil J and Mill T. (2002). Phylogenetic analysis in the genus Cicer and cultivated chickpea using RAPD and ISSR markers. Theoretical and Applied Genetics. 104:643-651. https://doi.org/10.1007/s001220100751
  13. Jeon SH, Son D, Ryu YS, Kim SH, Chung JI, Kim MC and Shim SI. (2010). Effect of presowing seed treatments on germination and seedling emergence in Taraxacum platycarpum. Korean Journal of Medicinal Crop Science. 18:9-14.
  14. Kang HS and Choi YM. (1998). Seasonal variation of reproductive characters in two introduced species of Taraxacum. Korean Journal of Ecology and Field Biology. 21:475-486.
  15. Kashin AS, Anfalov VE and Demochko YA. (2005). Studying allozyme variation in sexual and apomictic Taraxacum and Pilosella (Asteraceae) populations. Russian Journal of Genetics. 41:144-154. https://doi.org/10.1007/s11177-005-0038-4
  16. Keum YS. (1992). Taxonomic study of the genus Taraxacum Wiggers in Korea. M.A. thesis, Kyungpook National University. p. 1-4.
  17. Lee CB. (1993). Illustrated flora of Korea. Hyangmoonsa press. Seoul, Korea. p. 783-784
  18. Lee HH and Lee SY. (2008). Cytotoxic and antioxidant effects of Taraxacum coreanum Nakai. and T. officinale WEB. extracts. Korean Journal of Medicinal Crop Science. 16:79-85.
  19. Lee KH, Yang JY, Pak JH, Morita T and Ito M. (2004). Relationship of diploid East Aisan Taraxacum Wiggers using the capitulum morphological character. Korean Journal of Plant Taxonomy. 34:153-166. https://doi.org/10.11110/kjpt.2004.34.2.153
  20. Litt, M and Luty JA. (1989). A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. American Journal of Human Genetics. 44:388-396.
  21. Russel TR, Fuller JD, Macaulay M, Hatz BG, Jahoor A, Powell WP and Waugh R. (1997). Direct comparison of level of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theoretical and Applied Genetics. 95:714-722. https://doi.org/10.1007/s001220050617
  22. Ryu JH, Choi GL, Lyu JI, Lee SC, Chun JU, Shin DY and Bae CH. (2010). Genetic relationship analysis of genus Nelumbo accessions based on inter-simple sequence repeats (ISSR). Korean Journal of Medicinal Crop Science. 18:86-92.
  23. Wu W, Zheng YL, Chen L, Wei YM, Yang RW and Yan ZH. (2005). Evaluation of genetic relationships in the genus Houttuynia Thunb. in China based on RAPD and ISSR markers. Biochemical Systematics and Ecology. 33:1141-1157 https://doi.org/10.1016/j.bse.2005.02.010
  24. Yang HS. (1995). Studies of environmental responses in interspecific competition of ecotypes of Taraxacum officinale. Korean Journal of Environmental Biology. 13:107-120.
  25. Yuan CI, Lin LC, Lin CC, Kuo LC and Chiang MY. (2009). Allele-specific PCR and ISSR marker used in rapid detection of adulteration species of Taraxacum mongolicum. Crop, Environment & Bioinformatics. 6:183-191.
  26. Zietkiewicz E, Rafalski A and Labuda D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics. 20:176-183. https://doi.org/10.1006/geno.1994.1151

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