• Title/Summary/Keyword: Accelerated Life Test

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Rapid Cell Death Phenotype of Streptococcus mutans under Prolonged Growth Conditions (장시간 생장 조건에서 Streptococcus mutans의 급격한 세포사 표현형 분석)

  • Kim, Jeong Nam
    • Journal of Life Science
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    • v.31 no.12
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    • pp.1072-1078
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    • 2021
  • The oral pathogen Streptococcus mutans is considered a major causative agent of dental caries in humans. The use of dental hygiene products, including toothpaste and mouthwash, is used for caries control. However, food intake can lead to the recurrence of oral microorganisms. This study aimed to explore why this bacterium dies so quickly during prolonged incubation and to assess whether this growth characteristic is closely associated with the secretion of metabolic products. Notably, the number of live S. mutans cells rapidly declined after 24 hr during the entire period tested, whereas the number of Escherichia coli cells, an indicator strain, remained steady over the same period. To test whether the S. mutans supernatants contained possible signals that accelerated the death of neighbor cells, we obtained the individual supernatants at the above time points. Following pH neutralization, the cells in which the supernatant was supplemented with glucose grew well. However, pH adjustment alone could not fully recover cell growth in conditions in which the supernatant was supplemented, with or without glucose. These phenotypes of S. mutans may be associated with signaling, not only resulting from nutrient depletion. The findings on the survival phenotype of S. mutans provide new insights into cell-cell communication in the biology of this bacterium.

Summative Usability Assessment of Software for Ventilator Central Monitoring System (인공호흡기 중앙감시시스템 소프트웨어의 사용적합성 총괄평가)

  • Ji-Yong Chung;You Rim Kim;Wonseuk Jang
    • Journal of Biomedical Engineering Research
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    • v.44 no.6
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    • pp.363-376
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    • 2023
  • According to the COVID-19, development of various medical software based on IoT(Internet of Things) was accelerated. Especially, interest in a central software system that can remotely monitor and control ventilators is increasing to solve problems related to the continuous increase in severe COVID-19 patients. Since medical device software is closely related to human life, this study aims to develop central monitoring system that can remotely monitor and control multiple ventilators in compliance with medical device software development standards and to verify performance of system. In addition, to ensure the safety and reliability of this central monitoring system, this study also specifies risk management requirements that can identify hazardous situations and evaluate potential hazards and confirms the implementation of cybersecurity to protect against potential cyber threats, which can have serious consequences for patient safety. As a result, we obtained medical device software manufacturing certificates from MFDS(Ministry of Food and Drug Safety) through technical documents about performance verification, risk management and cybersecurity application.The purpose of this study is to conduct a usability assessment to ensure that ergonomic design has been applied so that the ventilator central monitoring system can improve user satisfaction, efficiency, and safety. The rapid spread of COVID-19, which began in 2019, caused significant damage global medical system. In this situation, the need for a system to monitor multiple patients with ventilators was highlighted as a solution for various problems. Since medical device software is closely related to human life, ensuring their safety and satisfaction is important before their actual deployment in the field. In this study, a total of 21 participants consisting of respiratory staffs conducted usability test according to the use scenarios in the simulated use environment. Nine use scenarios were conducted to derive an average task success rate and opinions on user interface were collected through five-point Likert scale satisfaction evaluation and questionnaire. Participants conducted a total of nine use scenario tasks with an average success rate of 93% and five-point Likert scale satisfaction survey showed a high satisfaction result of 4.7 points on average. Users evaluated that the device would be useful for effectively managing multiple patients with ventilators. However, improvements are required for interfaces associated with task that do not exceed the threshold for task success rate. In addition, even medical devices with sufficient safety and efficiency cannot guarantee absolute safety, so it is suggested to continuously evaluate user feedback even after introducing them to the actual site.

A Study on Utilizing Instrumented Indentation Technique for Evaluating In-field Integrity of Nuclear Structures (원전 구조물의 가동 중 건전성 평가를 위한 연속압입시험법의 활용에 관한 연구)

  • Song, Won-Seok;Kim, Seung-Gyu;Ahn, Hee-Jun;Kim, Kwang-Ho;Kwon, Dongil
    • Journal of the Korean Society for Nondestructive Testing
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    • v.33 no.2
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    • pp.165-172
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    • 2013
  • Power generating unit structures are designed and built to meet standard to secure its safety for expected life time. As the structures have been exposed to combined environment, degradation of structure material is accelerated and it can cause unexpected damage; evaluating precise mechanical properties of weak site like welded area is an essential research area as it is directly connected to safety issues. Existing measuring technique like tensile test requires specific size in testing specimen yet it is destructive method which is hard to apply on running structures. To overcome above mentioned limitation, IIT is getting limelight as it is non-destructive and simple method. In this study, latest technique is introduced to evaluate tensile property and residual stress by analyzing stress field occurs under the indenter while IIT is performed. Test on welded area, the weak site of nuclear structures have been practiced and confirmed that IIT can be usefully applied to evaluate integrity in industry.

Manufacturing Multi-degradable Food Packaging Films and Their Degradibility (복합분해성 플라스틱 식품포장 필름의 제조 및 분해성)

  • Chung, Myong-Soo;Lee, Wang-Hyun;You, Young-Sun;Kim, Hye-Young;Park, Ki-Moon
    • Korean Journal of Food Science and Technology
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    • v.35 no.5
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    • pp.877-883
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    • 2003
  • Multi-degradable master hatch (M/B) was prepared and 0.05 mm polyethylene (PP) food packaging films containing 0, 10, and 20% M/B were manufactured by inflation film processing. The films were exposed to UV radiation, fungi, and heat in order to observe their photolysis, biodegradability, and thermal degradability, respectively. While pure PP film maintained more than 70% of its original elongation after 8 weeks of UV radiation, an almost perfect loss in the elongation of PP film containing 20% M/B was observed. Significant decreases in elongation of PP films by heat treatment $(68{\pm}2^{\circ}C)$ were also found in samples containing the multi-degradable M/B. By observing changes in film surface after the inoculation of fungi using scanning electron microscopy (SEM), the biodegradability of plastic film could be accelerated with the addition of multi-degradable M/B. The results of the mulching test in yard showed that adding multi-degradable M/B can effectively degrade plastic films in natural environmental conditions without interrupting the growth of plants.

Reproductive Cycle of Natural Population and Artificial Control of Gonadal Development of Ruditapes philippinarum by the Conditions of Water Temperature-Feeding and Starvation (자연산 바지락, Ruditapes philippinarum의 생식주기와 수온-먹이섭이 및 절식조건에 의한 생식소발달의 인위적 제어)

  • Chung, Ee-Yung;Lee, Jung-Sik;Lee, Chang-Hoon;Hur, Sung-Bum
    • The Korean Journal of Malacology
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    • v.18 no.2
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    • pp.83-91
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    • 2002
  • Reproductive cycle of natural population and artificial control experiments of gonadal development by the conditions of water temperatures-feeding and starvation of Ruditapes philippinarum were investigated by histological observations. The reproductive cycle of natural population in females and males can be categorized into five successive stages; early active (February to March), late active (April to May), ripe (April to August), partially spawned (May to October), and spent-inactive stage (August to March). In the artificial control experiments, gonadal development of this species was inhibited by the low water temperature (10$^{\circ}C$). In the experimental group which was exposed to artificial high water temperatures of 19$^{\circ}C$ and 22$^{\circ}C$, gonadal development was accelerated by the higher water temperatures and was faster (about one month) than that in natural populations. In the high water temperatures-feeding experimental group, the gonadal developmental phase was faster in the small-size group than that in the large-size group, and was faster in lower water temperature (10$^{\circ}C$)(p=0.01). The gonad developmental phases in the high water temperature (22-28$^{\circ}C$)-starvation experimental group showed faster (paired sample t-test, p=0.004) than those in the high water temperature-feeding group in females and males. In the high water temperature-feeding experimental group of female and male gonadal developments of small sized group were more sensitive than those in large sized group after 42 days cultivation, However, the gonadal development of male was more sensitive to the lower water temperature than female. On the whole, sexual maturation in the high water temperature experimental group was faster than those in the low water temperature group, and showed a significant difference (paired sample t-test, p=0.001) between female and male. In the starvation experimental group after 42 days, gonadal developments in the high water temperature-large male group showed faster than those in the high water temperature-large female group. However, in small size, gonad developmental phases showed the same pattern between feeding and starvation experimental groups. During the main spawning season, in the high water temperature-starvation experimental groups in females and males, their gonadal development showed faster than that in higher water temperature-feeding experimental group regardless of their sexes and individual sizes and showed a significant difference (paired sample t-test, p=0.004).

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A Study on the Degradation Properties of DGEBA/TETA Epoxy System for Restoration of Ceramics by Temperature (도자기 복원용 DGEBA/TETA Epoxy계 수지의 온도에 의한 열화 특성 연구)

  • Nam, Byeong Jik;Jang, Sung Yoon
    • Journal of Conservation Science
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    • v.31 no.4
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    • pp.373-386
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    • 2015
  • This study identified degradation properties by temperature stress with Araldite$^{(R)}$ AY103-1/HY956 used for ceramics. Tensile and compressive strength of durability increased for 6,480 hours at temperature of $34{\sim}45^{\circ}C$. In stability of external stress and temperature, compressive strength is superior to tensile strength, it requires conservation plans considering strength properties and stress of restoration materials. The tensile shear strength of adhesion properties decreased for 4,320 hours at temperature of $40{\sim}60^{\circ}C$. In ceramics with porosity, environments under isothermal-isohumidity are important because interfacial properties of adherend are concerned with performance variation. Glossiness decreased for 6,480 hours at temperature of $34{\sim}45^{\circ}C$ and color difference increased. Gloss stability was superior and color stability was weak, which requires improvement of optical properties. In artifacts on display in museums, there is concern about temperature rise on restoration materials by lighting therefore, it needs to minimize change in physical properties by exposure environments.

Studies on the Adaptability of Home Made Retort Pouch for Food Packaging (국산(國産) Retort Pouch의 식품포장적성(食品包裝適性)에 관한 조사 연구)

  • Park, Moo-Hyun;Chung, Dong-Hyou;Kim, June-Pyung;Shin, Dong-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.13 no.1
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    • pp.15-21
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    • 1984
  • In order to ascertain the practical possibility of home made retort pouches for production of retort pouch foods in factory, two kinds of retortable pouches, i. e. 3 ply film (polyester film/Al. foil/casting polypropylene) and 4 ply film(polyester film/Al. foil/nylon/casting poll·propylene) were developed in Korea, and then, their physical properties, such as physical strength, heat resistance during thermal processing and preservability of foods and sanitary safety, were tested and compared with the foreign made pouches. The results obtained were summarized as follows. a. The physical strength of home made retort pouches in processing model foods(soybean oil 5 %, acetic acid 2%, starch 5 % and water to 100 %) was equal to that of foreign made pouches at accelerated examination condition($38^{\circ}C$, 98 % RH). But the lamination strength of home made products was a little bit superior to that of foreign made products. b. Home made retort pouches(SAMA-4 ply and SAMA-3 ply pouch) packed with rice or curry was possible to bear the thermal processing at $135^{\circ}C$ and shelf-life was one year or more at ambient temperature($20^{\circ}C$). c. Sanitary safety test showed that these home made retort pouches were acceptable comparing to the regulation recommended by the Ministry of Health and Social Affairs.

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Adhesion and Lifetime Extension Properties of Electrical Conductive Paint Stored under of Nitrogen Atmosphere (질소환경에서 보관된 전기전도성 페인트의 접착 및 수명연장 특성)

  • Shin, Pyeong-Su;Kim, Jong-Hyun;Baek, Yeong-Min;Park, Ha-Seung;Park, Joung-Man
    • Journal of Adhesion and Interface
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    • v.20 no.1
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    • pp.9-14
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    • 2019
  • The change of three different reagents for electrical conductive paint using aircraft coating with elapsing time of exposure to different condition was investigated. Three different reagents were poured into the vial bottles, stored in air condition and room temperature and observed with elapsing days. In addition, adhesion property of paint was tried using cross cut tape test after storage of $N_2$ atmosphere. The weight of each different reagent was measured along with elapsing time. To confirm the change of chemical component with exposure of air atmosphere, FT-IR was performed. The weight of part A and Part B decreased slightly whereas the weight of part C decreased rapidly and the precipitation was remained. The part B was cured after exposure of $N_2$ atmosphere and the 2250 cm-1 from FT-IR peak decreased slowly at the same time. It was considered that the water contained in air accelerated the reaction of -NCO functional groups and it caused the curing whereas $N_2$ atmosphere not contained water and it resulted in the retardancy of curing.

Corrosion Rate of Structural Pipes for Greenhouse (온실 구조용 파이프의 부식속도 검토)

  • Yun, Sung-Wook;Choi, Man Kwon;Lee, Si Young;Moon, Sung Dong;Yoon, Yong Cheol
    • Journal of Bio-Environment Control
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    • v.24 no.4
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    • pp.333-340
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    • 2015
  • Because soils in reclaimed lands nearby coastal areas have much higher salinity and moisture content than soils in inland area, parts of greenhouses embedded in such soils are exposed to highly corrosive environments. Owing to the accelerated corrosion of galvanized steel pipes for substrucrture and structure of greenhouses in saline environments, repair and reinforcement technologies and efficient maintenance and management for the construction materials in such facilities are required. In this study, we measured the corrosion rates of the parts used for greenhouse construction that are exposed to the saline environment to obtain a basic database for the establishment of maintenance and reinforcement standards for greenhouse construction in reclaimed lands with soils with high salinity. All the test pipes were exposed to soil and water environments with 0, 0.1, 0.3, and 0.5% salinity during the observation period of 480 days. At the end of the observation period, salinity-dependent differences of corrosion rate between black-surface corrosion and relatively regular corrosion were clearly manifested in a visual assessment. For the soils in rice paddies, the corrosion growth rate increased with salinity (0.008, 0.027, 0.036, and $0.043mm{\cdot}yr^{-1}$ at 0, 0.1, 0.3, and 0.5% salinity, respectively). The results for the soils in agricultural fields are 0.0002, 0.039, 0.040, and $0.039mm{\cdot}yr^{-1}$ at 0, 0.1, 0.3, and 0.5% salinity, respectively. The higher corrosion rate of rice-paddy soil was associated with the relatively high proportion of fine particles in it, reflecting the general tendency of soils with evenly distributed fine particles. Hence, it was concluded that thorough measures should be taken to counteract pipe corrosion, given that besides high salinity, the soils in reclaimed lands are expected to have a higher proportion of fine particles than those in inland rice paddies and agricultural fields.

Studies on the Quality Characteristics and Shelf-life of Maejackwa Containing Nuts and Seeds Prepared by Baking Method (굽는 방법으로 제조한 견과종실류 첨가 매작과의 품질특성 및 저장성에 관한 연구)

  • Chung, Hai-Jung;Kim, Sung-Joon;Baek, Jung-Eun;Sung, Dae-Kyung;Song, Hwa-Youn;Lee, Jin-Young;Kim, Gyu-Heun
    • Korean journal of food and cookery science
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    • v.24 no.6
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    • pp.811-817
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    • 2008
  • The principal objective of this study was to evaluate the effects of baking method on the quality and shelf-life of Maejakgwa containing nuts and seeds. Four different types of nuts and seeds (almond, cashewnut, coconut, and sunflower seed) were added to flour, and baking method was applied to prepare the Maejakgwa, physicochemical properties of which were then evaluated. The proximate composition analysis demonstrated that crude protein, crude fat, and ash contents were higher in the Maejakgwa containing nuts and seeds than in the control group. The lightness (L) value of the control Maejakgwa was lower than that observed in the groups containing nuts and seeds. Maejakgwa made with cashewnut, coconut, and sunflower seed evidenced higher yellowness (b) values than were recorded in the control and coconut groups. The incorporation of cashewnut, coconut and sunflower seed into the Maejakgwa lowered the hardness values (p<0.05). Our consumer test demonstrated that Maejakgwa prepared with almond was the most highly preferred, and the control group was the least favored. After 30 days of storage at $60^{\circ}C$, the peroxide value of the Maejakgwa containing sunflower seed was substantially higher than in any other groups, thereby suggesting that the addition of sunflower seeds resulted in accelerated lipid oxidation. No noticeable changes in peroxide values were observed in the groups prepared with coconut and cashewnut during storage time. The acid value increased with increasing storage time, and the addition of sunflower seed evidenced the highest values as compared to other groups.